A nanofluidic device for ultrasensitive and label-free detection of tetracycline in association with γ-cyclodextrin and GO

2021 ◽  
Vol 13 (15) ◽  
pp. 1832-1838
Author(s):  
Jiaxi Cheng ◽  
Fenghua Jiang ◽  
Siqi Zhang
Keyword(s):  
Detection Limit ◽  
Water Samples ◽  
Recovery Rate ◽  
Label Free ◽  
Detection Range ◽  
Low Detection Limit ◽  
Sensing Platform ◽  
Label Free Detection ◽  
Nanofluidic Device

We develop a label-free, sensitive and selective nanochannel sensing platform for detection of TC. The nanosensor provided a low detection limit, a wide detection range and excellent recovery rate in different water samples.

Deep Learning-enabled Holographic Imaging Flow-Cytometry for Label-Free Detection of Giardia Lamblia in Water Samples

2021 ◽  
Author(s):  
Zoltán Göröcs ◽  
David Baum ◽  
Fang Song ◽  
Kevin de Haan ◽  
Hatice Ceylan Koydemir ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Deep Learning ◽  
Water Samples ◽  
Giardia Lamblia ◽  
Label Free ◽  
Holographic Imaging ◽  
Imaging Flow Cytometry ◽  
Label Free Detection

scholarly journals An Aptamer-Based Capacitive Sensing Platform for Specific Detection of Lung Carcinoma Cells in the Microfluidic Chip

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 98 ◽  
Author(s):  
Ngoc-Viet Nguyen ◽  
Chun-Hao Yang ◽  
Chung-Jung Liu ◽  
Chao-Hung Kuo ◽  
Deng-Chyang Wu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Carcinoma ◽  
Early Stage ◽  
A549 Cells ◽  
Microfluidic Channel ◽  
Electrical Impedance Spectroscopy ◽  
Label Free ◽  
Early Stage Lung Cancer ◽  
Sensing Platform ◽  
Label Free Detection

Improvement of methods for reliable and early diagnosis of the cellular diseases is necessary. A biological selectivity probe, such as an aptamer, is one of the candidate recognition layers that can be used to detect important biomolecules. Lung cancer is currently a typical cause of cancer-related deaths. In this work, an electrical sensing platform is built based on amine-terminated aptamer modified-gold electrodes for the specific, label-free detection of a human lung carcinoma cell line (A549). The microdevice, that includes a coplanar electrodes configuration and a simple microfluidic channel on a glass substrate, is fabricated using standard photolithography and cast molding techniques. A procedure of self-assembly onto the gold surface is proposed. Optical microscope observations and electrical impedance spectroscopy measurements confirm that the fabricated microchip can specifically and effectively identify A549 cells. In the experiments, the capacitance element that is dominant in the change of the impedance is calculated at the appropriate frequency for evaluation of the sensitivity of the biosensor. Therefore, a simple, inexpensive, biocompatible, and selective biosensor that has the potential to detect early-stage lung cancer would be developed.

scholarly journals Comparison of DNAzyme activity for the development of an immobilized heme sensor

MRS Advances ◽  
2018 ◽  
Vol 3 (26) ◽  
pp. 1491-1496
Author(s):  
Natalie Hughes ◽  
Nancy Nguyen ◽  
Deanna-Kaye Daley ◽  
Justin Grennell ◽  
Amira Gee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Label Free ◽  
Detection Range ◽  
Chemical Complexity ◽  
Detection Strategy ◽  
Detection Approach ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Care Systems

ABSTRACTPoint-of-care systems require highly sensitive, quantitative and selective detection platforms for the real-time multiplexed monitoring of target analytes. To ensure facile development of a sensor, it is preferable for the detection assay to have minimal chemical complexity, contain no wash steps and provide a wide and easily adaptable detection range for multiple targets. Current studies involve label-free detection strategy for relevant clinical molecules such as heme using G-quadruplex based self-assembly. We have explored the measurement of binding and kinetic parameters of various G-quadruplex/heme complexes which are able to self-associate to form a DNAzyme with peroxidase mimicking capabilities and are critical to nucleic acid research. The detection strategy includes immobilizing the G-quadruplex sequences within a polymer matrix to provide a self-assembly based detection approach for heme that could be translated towards other clinically relevant targets.

Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of DNA

The Analyst ◽  
2016 ◽  
Vol 141 (15) ◽  
pp. 4719-4724 ◽  
Author(s):  
Yaqing Chang ◽  
Zhe Zhang ◽  
Huiqing Liu ◽  
Nan Wang ◽  
Jilin Tang
Keyword(s):  
Fluorescence Quenching ◽  
Label Free ◽  
Quenching Mechanism ◽  
Fluorescence Sensing ◽  
Single Stranded Dna ◽  
Cobalt Oxyhydroxide ◽  
Sensing Platform ◽  
Label Free Detection ◽  
Fluorescence Quenching Mechanism ◽  
Coooh Nanoflakes

In this study, we investigated the interaction of cobalt oxyhydroxide (CoOOH) nanoflakes with DNA and their fluorescence quenching mechanism of a FAM-labeled single-stranded DNA (ssDNA) probe.

A Comparative Study on the Analysis Methods for Chlorophyll-a

2013 ◽  
Vol 726-731 ◽  
pp. 1411-1415 ◽  
Author(s):  
Fei Peng ◽  
Shi Bo Liu ◽  
Hai Yan Xu ◽  
Zhi Qun Li
Keyword(s):  
Detection Limit ◽  
Chlorophyll A ◽  
Water Samples ◽  
Chlorophyll A Fluorescence ◽  
Standard Procedure ◽  
Fluorescence Method ◽  
Monitoring Method ◽  
Detection Range ◽  
Analysis Methods

This paper investigated three chlorophyll-a analysis methods in water sample. The UV-VIS spectrophotometry method followed SL88-2012 standard procedure, the results showed the detection limit of chlorophyll-a was 0.22 μg/L, with the lower detection limit of 1.0 μg/L. The detection range of the vivo chlorophyll-a fluorescence method was 0.1-300μg/L, the ratios of different algae matched that prepared in water samples. The detection limits and quantification limits for HPLC by using UVD and FLD were respectively 0.013 and 0.004 μg/L, 0.040 and 0.013μg/L. FLD was more sensitive than UVD for chlorophyll-a analysis, and the recovery of standard addition was in the range of 90.2% to 103.9% with UVD and FLD. Given the different pretreatment procedures of the sample, equipment conditions, correction and precision, vivo chlorophyll-a fluorescence method was the best on-site monitoring and the emergency monitoring method. Spectrophotometer was suitable for routine laboratory determination of chlorophyll-a, especially for bulk water samples. HPLC method had high precision and sensitivity, so this method applied to the determination of exact requirements, microanalysis and pigment separation.

Label-Free Aptamer Fluorescence Determination of Trace Pb2+ Using AuPd Nanoalloy Probe as Catalyst

2013 ◽  
Vol 631-632 ◽  
pp. 18-21 ◽  
Author(s):  
Zhi Liang Jiang ◽  
Mei Ling Tang ◽  
Qing Ye Liu ◽  
Ai Hui Liang
Keyword(s):  
Detection Limit ◽  
Fluorescence Quenching ◽  
Fluorescence Intensity ◽  
Water Samples ◽  
Catalytic Effect ◽  
Rhodamine 6G ◽  
Label Free ◽  
Double Stranded Dna ◽  
Fluorescence Determination

In the condition of 1.24 mmol/L EDTANa2, 16.7 mmol/L NaCl and 0.17 mmol/L Tris, the substrate chain of double-stranded DNA (dsDNA) could be cracked by Pb2+ to release single-stranded DNA (ssDNA) that adsorb onto AuPd nanoparticle (AuPdNP) and form stable AuPdNP-ssDNA, but the dsDNA can not protect AuPdNP that were aggregated to big AuPdNP aggregations (AuPdNPA) under the action of NaCl. The AuPdNP-ssDNA and AuPdNPA could be separated by centrifugation. With the concentration of Pb2+ increased, the released ssDNA increased, the AuPdNP-ssDNA in centrifugation solution increased and the catalytic effect enhanced on the fluorescence quenching reaction of Rhodamine 6G (Rh6G) and NaH2PO2, which led the fluorescence intensity at 552nm to decrease. The decreased fluorescence intensity (ΔF552nm) was linear to the concentration of Pb2+ in the range of 0.33-8.00 nmol/L, a detection limit of 0.21 nmol/L. The proposed method was applied to detect Pb2+ in water samples, with satisfactory results.

scholarly journals Nanostructured Aptamer-Functionalized Black Phosphorus Sensing Platform for Label-Free Detection of Myoglobin, a Cardiovascular Disease Biomarker

2016 ◽  
Vol 8 (35) ◽  
pp. 22860-22868 ◽  
Author(s):  
Vinod Kumar ◽  
Jack R. Brent ◽  
Munish Shorie ◽  
Harmanjit Kaur ◽  
Gaganpreet Chadha ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Black Phosphorus ◽  
Label Free ◽  
Disease Biomarker ◽  
Sensing Platform ◽  
Label Free Detection

scholarly journals Combination of Capped Gold Nanoslit Array and Electrochemistry for Sensitive Aqueous Mercuric Ions Detection

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 88
Author(s):  
Cheng-Chuan Chen ◽  
Shu-Cheng Lo ◽  
Pei-Kuen Wei
Keyword(s):  
Metal Ion ◽  
Water Solution ◽  
Resonant Peak ◽  
Label Free ◽  
Glycerol Water ◽  
Detection Range ◽  
Label Free Detection ◽  
Intensity Changes ◽  
Mercuric Ions ◽  
Nanoslit Arrays

Label-free surface plasmon resonance (SPR) detection of mercuric ions in various aqueous solutions, using capped gold nanoslit arrays combined with electrochemical (EC) sensing technique, is demonstrated. The nanoslit arrays are fabricated on flexible cyclo-olefin polymer substrates by a nanoimprinting lithography method. The EC and SPR signals for the investigation of current responses and transmission SPR spectra are simultaneously measured during metal ions electrodeposition. Glycerol–water solution is studied to evaluate the resonant peak wavelength sensitivity (480.3 nm RIU−1) with a FOM of 40.0 RIU−1 and the obtained intensity sensitivity is 1819.9%. The ferrocyanide/ferricyanide redox couple performs the diffusion controlled electrochemical processes (R2 = 0.99). By investigating the SPR intensity changes and wavelength shifts of various mercuric ion concentrations, the optical properties are evaluated under chronoamperometric conditions. The sensors are evaluated in the detection range between 100 μM and 10 nM with a detection limit of 1 μM. The time dependence of SPR signals and the selectivity of 10 μM Hg2+ in the presence of 10 μM interfering metal ion species from Ca2+, Co2+, Ni2+, Na+, Cu2+, Pb2 + and Mn2+ are determined. The capped gold nanoslit arrays show the selectivity of Hg2+ and the EC sensing method is effectively utilized to aqueous Hg2+ detection. This study provides a label-free detection technique of mercuric ions and this developed system is potentially applicable to detecting chemicals and biomolecules.

Fiber-optics: a new route towards ultra-low detection limit label-free biosensing

2019 ◽  
Author(s):  
Pablo Zubiate ◽  
Desiree Santano ◽  
Aitor Urrutia ◽  
Carlos R. Zamarreño ◽  
Silvia Díaz ◽  
...  
Keyword(s):  
Detection Limit ◽  
Fiber Optics ◽  
Label Free ◽  
Low Detection Limit
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