Direct detection of inorganic ions and underivatized amino acids in seconds using high-speed capillary electrophoresis coupled with back-scatter interferometry

2021 ◽  
Author(s):  
Miyuru De Silva ◽  
Prabhavie M. Opallage ◽  
Robert C. Dunn
Keyword(s):  
Amino Acids ◽  
Capillary Electrophoresis ◽  
Amino Acid ◽  
High Speed ◽  
Direct Detection ◽  
Inorganic Ions ◽  
Short Length ◽  
Thin Wall ◽  
Underivatized Amino Acids

A short length, ultra-thin wall separation capillary combined with back-scatter interferometry enables amino acid separation and detection in seconds.

scholarly journals Suitability of sugar, amino acid, and inorganic ion compositions to distinguish fir and spruce honey

2021 ◽  
Author(s):  
Basel Shaaban ◽  
Victoria Seeburger ◽  
Annette Schroeder ◽  
Gertrud Lohaus
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Picea Abies ◽  
Multivariate Analyses ◽  
Abies Alba ◽  
Inorganic Ions ◽  
Scale Insects ◽  
Inorganic Ion ◽  
Plant Feeding ◽  
Different Types

AbstractHoneydew honey is produced by bees from excretions of plant-feeding insects, such as aphids and scale insects. Honeydew on conifers, like fir (Abies alba) or spruce (Picea abies), is produced by different species of the genera Cinara and Physokermes. This means that honeydew honey can stem from different botanical as well as zoological origins, but so far it is not possible to clearly distinguish the different types of honeys. In the attempt to identify distinguishing markers, 19 sugars, 25 amino acids and 9 inorganic ions were quantified in three groups of honeydew honey (fir/Cinara, spruce/Cinara and spruce/Physokermes) with 20 honey samples each. It could be demonstrated that the contents of isomaltose, raffinose, erlose, two undefined oligosaccharides, several amino acids, sulfate, and phosphate differed significantly between the three groups of honey. Furthermore, multivariate analyses resulted in a separation of spruce/Physokermes honey from spruce- or fir/Cinara honey due to its higher contents of phosphate, sulfate, erlose and two undefined oligosaccharides. Moreover, the amino acid composition and the isomaltose as well as the raffinose contents proved useful in the distinction between fir/Cinara and spruce/Cinara honey. In sum, the contents of sugars, amino acids, and inorganic ions in German fir and spruce honeys provide useful information about the botanical and zoological origin of honeydew honeys.

The Composition of the Seminal Plasma of Balanus Balanus

1962 ◽  
Vol 39 (3) ◽  
pp. 345-352
Author(s):  
H. BARNES
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Amino Acid ◽  
Seminal Plasma ◽  
Free Amino ◽  
Reducing Sugars ◽  
Sea Water ◽  
Protective Action ◽  
Inorganic Ions ◽  
Chemical Analyses

1. The results of some chemical analyses for inorganic and organic constituents of the seminal plasma of Balanus balanus are presented. 2. The inorganic ions show a cation deficit of 178 m-equiv./l., which is probably made up by free amino-acids. 3. Cystine is a prominent amino acid present. 4. Potassium and calcium are present in excess of their quantities in sea water. 5. Reducing sugars, compared with the amount found in sea-urchin spermatozoa, are found in moderate quantities, 1 mg./ml. 6. Phosphorus of all kinds is present in only small quantities (total of o.14 mg./ml.). 7. Some phosphatases are present. 8. There are 21 /µg./ml. of ascorbic acid; the function of this is discussed in relation to its possible contribution to the protective action against the poisoning of -SH groups by thiol-reactive agents.

scholarly journals Profiling of Amino Acids in Urine Samples of Patients Suffering from Inflammatory Bowel Disease by Capillary Electrophoresis-Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3345 ◽  
Author(s):  
Juraj Piestansky ◽  
Dominika Olesova ◽  
Jaroslav Galba ◽  
Katarina Marakova ◽  
Vojtech Parrak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Inflammatory Bowel Disease ◽  
Capillary Electrophoresis ◽  
Amino Acid ◽  
Bowel Disease ◽  
Urine Samples ◽  
Simultaneous Action ◽  
Relative Standard ◽  
Inflammatory Bowel

Urine represents a convenient biofluid for metabolomic studies due to its noninvasive collection and richness in metabolites. Here, amino acids are valuable biomarkers for their ability to reflect imbalances of different biochemical pathways. An impact of amino acids on pathology, prognosis and therapy of various diseases, including inflammatory bowel disease (IBD), is therefore the subject of current clinical research. This work is aimed to develop a capillary electrophoresis-tandem mass spectrometry (CE-MS/MS) method for the quantification of the 20 proteinogenic amino acids in human urine samples obtained from patients suffering from IBD and treated with thiopurines. The optimized CE-MS/MS method, with minimum sample preparation (just “dilute and shoot”), exhibited excellent linearity for all the analytes (coefficients of determination were higher than 0.99), with inter-day and intra-day precision yielding relative standard deviations in the range of 0.91–15.12% and with accuracy yielding relative errors in the range of 85.47–112.46%. Total analysis time, an important parameter for the sample throughput demanded in routine practice, was shorter in ca. 17% when compared to established CE-MS methods. Favorable performance of the proposed CE-MS/MS method was also confirmed by the comparison with corresponding ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method. Consistent data for the investigated amino acid metabolome were obtained using both methods. For the first time, the amino acid profiling by CE-MS approach was applied on the clinical IBD samples. Here, significant differences observed in the concentration levels of some amino acids between IBD patients undergoing thiopurine treatment and healthy volunteers could result from the simultaneous action of the disease and the corresponding therapy. These findings indicate that amino acids analysis could be a valuable tool for the study of mechanism of the IBD treatment by thiopurines.

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