scholarly journals Low-cost microphysiological systems: feasibility study of a tape-based barrier-on-chip for small intestine modeling

Lab on a Chip ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 1212-1226 ◽  
Author(s):  
Thomas E. Winkler ◽  
Michael Feil ◽  
Eva F. G. J. Stronkman ◽  
Isabelle Matthiesen ◽  
Anna Herland
Keyword(s):  
Small Intestine ◽  
Feasibility Study ◽  
Low Cost ◽  
Low Resource ◽  
Microphysiological Systems ◽  
On Chip ◽  
Chili Peppers

High costs are a key challenge in “democratization” of organ-chip research. We present a low-resource barrier-on-chip based on tape, and use it to model small intestine and its response to chili peppers (capsaicinoids).

scholarly journals Low-cost microphysiological systems: Feasibility study of a tape-based barrier-on-chip system for small intestine modeling

2020 ◽  
Author(s):  
Thomas E. Winkler ◽  
Michael Feil ◽  
Eva F.G.J. Stronkman ◽  
Isabelle Matthiesen ◽  
Anna Herland
Keyword(s):  
Small Intestine ◽  
Pore Size ◽  
Biological Effects ◽  
Low Cost ◽  
Adhesive Tape ◽  
Pressure Sensitive Adhesive ◽  
Laboratory Equipment ◽  
Chip Fabrication ◽  
On Chip ◽  
Permeable Support

AbstractWe see affordability as a key challenge in making organs-on-chips accessible to a wider range of users, particularly outside the highest-resource environments. Here, we present an approach to barrier-on-a-chip fabrication based on double-sided pressure-sensitive adhesive tape and off-the-shelf polycarbonate. Besides a low materials cost, common also to PDMS or thermoplastics, it requires minimal (€ 100) investment in laboratory equipment, yet at the same time is suitable for upscaling to industrial roll-to-roll manufacture. We evaluate our microhpysiological system with an epithelial (C2BBe1) barrier model of the small intestine, studying the biological effects of permeable support pore size, as well as stimulation with a common food compound (chili pepper-derived capsaicinoids). The cells form tight and continuous barrier layers inside our systems, with comparable permeability but superior epithelial polarization compared to Transwell culture, in line with other perfused microphysiological models. Permeable support pore size is shown to weakly impact barrier layer integrity as well as the metabolic cell profile. Capsaicinoid response proves distinct between culture systems, but we show that impacted metabolic pathways are partly conserved, and that cytoskeletal changes align with previous studies. Overall, our tape-based microphysiolgical system proves to be a robust and reproducible approach to studying physiological barriers, in spite of its low cost.

scholarly journals Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 110 ◽  
Author(s):  
Erika Ferrari ◽  
Cecilia Palma ◽  
Simone Vesentini ◽  
Paola Occhetta ◽  
Marco Rasponi
Keyword(s):  
Imaging Techniques ◽  
Biological Models ◽  
Electromechanical Properties ◽  
Human Organs ◽  
Tissue Constructs ◽  
Microphysiological Systems ◽  
Metabolic Activities ◽  
On Chip ◽  
Chip Analysis

Organs-on-chip (OoC), often referred to as microphysiological systems (MPS), are advanced in vitro tools able to replicate essential functions of human organs. Owing to their unprecedented ability to recapitulate key features of the native cellular environments, they represent promising tools for tissue engineering and drug screening applications. The achievement of proper functionalities within OoC is crucial; to this purpose, several parameters (e.g., chemical, physical) need to be assessed. Currently, most approaches rely on off-chip analysis and imaging techniques. However, the urgent demand for continuous, noninvasive, and real-time monitoring of tissue constructs requires the direct integration of biosensors. In this review, we focus on recent strategies to miniaturize and embed biosensing systems into organs-on-chip platforms. Biosensors for monitoring biological models with metabolic activities, models with tissue barrier functions, as well as models with electromechanical properties will be described and critically evaluated. In addition, multisensor integration within multiorgan platforms will be further reviewed and discussed.

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

On-Chip Fabrication of None-Dead-Volume Microtips for ESI-MS Applications

2007 ◽  
Vol 121-123 ◽  
pp. 611-614
Author(s):  
Che Hsin Lin ◽  
Jen Taie Shiea ◽  
Yen Lieng Lin
Keyword(s):  
Surface Tension ◽  
Low Cost ◽  
Microfluidic Channel ◽  
Dead Volume ◽  
Esi Ms ◽  
Rapid Fabrication ◽  
Chip Fabrication ◽  
Novel Method ◽  
On Chip ◽  
Highly Uniform

This paper proposes a novel method to on-chip fabricate a none-dead-volume microtip for ESI-MS applications. The microfluidic chip and ESI tip are fabricated in low-cost plastic based materials using a simple and rapid fabrication process. A constant-speed-pulling method is developed to fabricate the ESI tip by pulling mixed PMMA glue using a 30-μm stainless wire through the pre-formed microfluidic channel. The equilibrium of surface tension of PMMA glue will result in a sharp tip after curing. A highly uniform micro-tip can be formed directly at the outlet of the microfluidic channel with minimum dead-volume zone. Detection of caffeine, myoglobin, lysozyme and cytochrome C biosamples confirms the microchip device can be used for high resolution ESI-MS applications.

Optical MEMS platform for low-cost on-chip integration of planar light circuits and optical switching

2004 ◽  
Author(s):  
Kristine A. German ◽  
Joel Kubby ◽  
Jingkuang Chen ◽  
James Diehl ◽  
Kathleen Feinberg ◽  
...  
Keyword(s):  
Optical Switching ◽  
Low Cost ◽  
Optical Mems ◽  
On Chip

Feasibility study of cadmium adsorption by palm oil fuel ash (POFA)-based low-cost hollow fibre zeolitic membrane

2018 ◽  
Vol 25 (22) ◽  
pp. 21644-21655 ◽  
Author(s):  
Mohamad Sukri Mohamad Yusof ◽  
Mohd Hafiz Dzarfan Othman ◽  
Azeman Mustafa ◽  
Mukhlis Abdul Rahman ◽  
Juhana Jaafar ◽  
...  
Keyword(s):  
Feasibility Study ◽  
Palm Oil ◽  
Low Cost ◽  
Hollow Fibre ◽  
Palm Oil Fuel Ash ◽  
Fuel Ash ◽  
Cadmium Adsorption ◽  
Oil Fuel

scholarly journals The Rise of the OM-LoC: Opto-Microfluidic Enabled Lab-on-Chip

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1467
Author(s):  
Harry Dawson ◽  
Jinane Elias ◽  
Pascal Etienne ◽  
Sylvie Calas-Etienne
Keyword(s):  
Low Cost ◽  
Optical Components ◽  
New Era ◽  
Lab On Chip ◽  
Highly Sensitive ◽  
Multiple Challenges ◽  
On Chip ◽  
Optical Circuits ◽  
Made In

The integration of optical circuits with microfluidic lab-on-chip (LoC) devices has resulted in a new era of potential in terms of both sample manipulation and detection at the micro-scale. On-chip optical components increase both control and analytical capabilities while reducing reliance on expensive laboratory photonic equipment that has limited microfluidic development. Notably, in-situ LoC devices for bio-chemical applications such as diagnostics and environmental monitoring could provide great value as low-cost, portable and highly sensitive systems. Multiple challenges remain however due to the complexity involved with combining photonics with micro-fabricated systems. Here, we aim to highlight the progress that optical on-chip systems have made in recent years regarding the main LoC applications: (1) sample manipulation and (2) detection. At the same time, we aim to address the constraints that limit industrial scaling of this technology. Through evaluating various fabrication methods, material choices and novel approaches of optic and fluidic integration, we aim to illustrate how optic-enabled LoC approaches are providing new possibilities for both sample analysis and manipulation.

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