A nano-detection-system based on chemical probe for early diagnosis of Atherosclerosis in situ

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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Polarity-active NIR probes with strong two-photon absorption and ultrahigh binding affinity of insulin amyloid fibrils

Chemical Science ◽

10.1039/d0sc03907a ◽

2021 ◽

Author(s):

Li Li ◽

Zheng Lv ◽

Zhongwei Man ◽

Zhenzhen Xu ◽

YuLing Wei ◽

...

Keyword(s):

Neurodegenerative Diseases ◽

Binding Affinity ◽

Fluorescent Probes ◽

Amyloid Fibrils ◽

Photon Absorption ◽

Two Photon Absorption ◽

Two Photon ◽

Medical Diagnostic

Amyloid fibrils are associated with many neurodegenerative diseases. In-situ and in-vivo visualization of amyloid fibrils is important for medical diagnostic and requires fluorescent probes with both excitation and emission wavelengths in...

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Mammographic microcalcifications and risk of breast cancer

British Journal of Cancer ◽

10.1038/s41416-021-01459-x ◽

2021 ◽

Author(s):

Shadi Azam ◽

Mikael Eriksson ◽

Arvid Sjölander ◽

Marike Gabrielson ◽

Roxanna Hellgren ◽

...

Keyword(s):

Breast Cancer ◽

Postmenopausal Women ◽

Detection System ◽

Premenopausal Women ◽

Computer Aided Detection ◽

Comprehensive Information ◽

Estimated Risk ◽

Logistic Regressions ◽

Microcalcification Clusters

Abstract Background Mammographic microcalcifications are considered early signs of breast cancer (BC). We examined the association between microcalcification clusters and the risk of overall and subtype-specific BC. Furthermore, we studied how mammographic density (MD) influences the association between microcalcification clusters and BC risk. Methods We used a prospective cohort (n = 53,273) of Swedish women with comprehensive information on BC risk factors and mammograms. The total number of microcalcification clusters and MD were measured using a computer-aided detection system and the STRATUS method, respectively. Cox regressions and logistic regressions were used to analyse the data. Results Overall, 676 women were diagnosed with BC. Women with ≥3 microcalcification clusters had a hazard ratio [HR] of 2.17 (95% confidence interval [CI] = 1.57–3.01) compared to women with no clusters. The estimated risk was more pronounced in premenopausal women (HR = 2.93; 95% CI = 1.67–5.16). For postmenopausal women, microcalcification clusters and MD had a similar influence on BC risk. No interaction was observed between microcalcification clusters and MD. Microcalcification clusters were significantly associated with in situ breast cancer (odds ratio: 2.03; 95% CI = 1.13–3.63). Conclusions Microcalcification clusters are an independent risk factor for BC, with a higher estimated risk in premenopausal women. In postmenopausal women, microcalcification clusters have a similar association with BC as baseline MD.

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Progress in radionuclide characterisation in marine sediments using an underwater gamma-ray spectrometer in 2π geometry

HNPS Proceedings ◽

10.12681/hnps.2000 ◽

2019 ◽

Vol 21 ◽

pp. 29

Author(s):

E. G. Androulakaki ◽

C. Tsabaris ◽

M. Kokkoris ◽

G. Eleftheriou ◽

D. L. Patiris ◽

...

Keyword(s):

Gamma Ray ◽

Detection System ◽

Full Energy Peak ◽

Gamma Ray Spectrometry ◽

Peak Efficiency ◽

Full Energy ◽

Effective Manner ◽

Energy Peak ◽

Full Energy Peak Efficiency

The in-situ gamma-ray spectrometry is a well suited method for seabed mapping applications, since it provides rapid results in a cost effective manner. Moreover, the in-situ method is preferable to the commonly applied laboratory measurements, due to its beneficial characteristics. Therefore, the development of in-situ systems for seabed measurements continuously grows. However, an efficiency calibration of the detection system is necessary for obtaining quantitative results in the full spectral range. In the present work, an approach for calculating the full-energy peak efficiency of an underwater insitu spectrometer for measure- ments on the seabed is presented. The experimental work was performed at the coastal site of Vasilikos (Cyprus). The experimental full-energy peak efficiency of the in-situ was determined in the energy range 1400–2600 keV, by combining the in-situ and laboratory reference measurements. The experimental effi- ciency results were theoretically reproduced by means of Monte Carlo (MC) simulations, using the MCNP5 code.

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Advances in Medical Diagnostic Technology

10.1007/978-981-4585-72-9 ◽

2014 ◽

Cited By ~ 4

Author(s):

Khin Wee Lai ◽

Yan Chai Hum ◽

Maheza Irna Mohamad Salim ◽

Sang-Bing Ong ◽

Nugraha Priya Utama ◽

...

Keyword(s):

Medical Diagnostic ◽

Diagnostic Technology

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Complete capillary electrophoresis process on a drone: towards a flying micro-lab

Analytical Methods ◽

10.1039/d0ay01220c ◽

2020 ◽

Vol 12 (41) ◽

pp. 4977-4986 ◽

Cited By ~ 3

Author(s):

Tomas Drevinskas ◽

Audrius Maruška ◽

Valdas Girdauskas ◽

Gediminas Dūda ◽

Jelena Gorbatsova ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Detection System ◽

Conductivity Detection ◽

Contactless Conductivity Detection ◽

Automated Sampling

A drone-mountable capillary electrophoresis-contactless conductivity detection system performs automated sampling and analysis in situ.

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CONTINUOUS RADON-IN-WATER MONITORING—COMPARISON OF METHODS UNDER LABORATORY CONDITIONS AND RESULTS OF IN SITU MEASUREMENTS

Radiation Protection Dosimetry ◽

10.1093/rpd/ncz241 ◽

2019 ◽

Vol 186 (2-3) ◽

pp. 406-412

Author(s):

Petra Vyletělová ◽

Aleš Froňka

Keyword(s):

Response Time ◽

Detection System ◽

Response Delay ◽

Detection Systems ◽

Water Detection ◽

Radon Diffusion ◽

Flow Through ◽

Continuous Monitor ◽

Radon In Water

Abstract The extraction of dissolved radon from water to gas is the most common way to measure radon concentration in water continuously. The response delay of continuous radon-in-water detection system (continuous monitor + equilibrator) is influenced by the response time of the continuous monitor and a rate of an establishment of equilibrium in the equilibrator (exchanger unit). Two types of equilibrators were used in performed experiments to compare the response time of various detection systems—RAD AQUA that uses water spraying and equilibrator with ACCUREL® PP membrane that enables radon diffusion. Each of these was connected to the continuous monitor RAD7 or RM-3. The response delay after turning on the water flow through the equilibrator was determined. The fastest detection system was RAD7 + RAD AQUA that was subsequently tested during the insitu measurement of thermal water in the healing spa and water sources near Cheb and České Budějovice.

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