Developing three-input cascade DNA logic gate based on biological characteristics of metal ion-GO combined with the analysis and verification

2021 ◽  
Author(s):  
Luhui Wang ◽  
Mengyang Hu ◽  
Yue Wang ◽  
Sunfan Xi ◽  
Meng Cheng ◽  
...  

Due to the limitation of technology, electronic computing is approaching the limit of technology, and new computing tools need to be developed. Here, we build a three input cascade logic...


2019 ◽  
Vol 95 (1-2) ◽  
pp. 79-89 ◽  
Author(s):  
Chitra Varadaraju ◽  
Mosae Selvakumar Paulraj ◽  
G. Tamilselvan ◽  
I. V. Muthu Vijayan Enoch ◽  
V. Srinivasadesikan ◽  
...  


2006 ◽  
Vol 3 (3) ◽  
pp. 171-176
Author(s):  
Yuan Zhe-Ming ◽  
Meng Xiao-Lin ◽  
Liu Shu-Sheng

AbstractA recombinant enhancin fromTrichoplusia nigranulovirus, expressed inEscherichia coliand named P96, had been shown in a previous study to increase significantly the efficacy of bio-insecticides applied to insect pests. Here, several biological characteristics were clarified to provide further proof for the activities of P96. The inclusion bodies formed by P96 were quite stable when expressed inE. coli; however, they could be effectively dissolved in sodium carbonate (Na2CO3) solution or in the midgut digestive secretion of the fifth-instar larvae of the cotton bollworm,Helicoverpa armigera,in vitro. According to sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE), the peritrophic membrane of the cotton bollworm was clearly decomposed by P96. With the increase of P96 concentration, the mortality of the cotton bollworm caused by nuclear polyhedrosis virus increased up to its saturation concentration, 6.76×105inclusion bodies/ml. The bioactivity of P96 was strongly inhibited by ethylenediaminetetraacetic acid (EDTA), which formed a complex compound with the metal ion (Zn2+) in the protein.



Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3091
Author(s):  
Xi-Ying Cao ◽  
Chu-Ming Pang ◽  
Ying Xiao ◽  
Wan-Qing Xiao ◽  
Shi-He Luo ◽  
...  

A new type of conjugated polybenzimidazole (CPBI) was synthesized through a simple polycondensation reaction without metal catalysis, and N-alkylation modification was carried out to solve the problems of solubility and fluorescence properties. A series of nano-microsphere polymers CPBIn with large conjugation, good solubility, and strong fluorescence has been successfully used as “turn-off” fluorescent probes for the first time. The results show that, under suitable N-alkylation conditions, the obtained CPBIn can be used as a highly sensitive and selective fluorescent probe for the detection of Cu2+ and Zn2+ at the same time, and their detection limits are both nM levels. In addition, CPBI2 can be designed as an ultra-sensitive IMPLICATION logic gate at the molecular level, cyclically detecting Cu2+. With the test paper containing CPBI2, easy and quick on-site detection can be achieved. This research provides a new idea for the brief synthesis of multifunctional materials.



The Analyst ◽  
2016 ◽  
Vol 141 (10) ◽  
pp. 2870-2873 ◽  
Author(s):  
Fubing Xiao ◽  
Hui Tan ◽  
Yan Wu ◽  
Shuzhen Liao ◽  
Zhaoyang Wu ◽  
...  

A novel LC-based DNA logic gate was constructed utilizing the reorientation of LCs triggered by metal-ion-mediated DNA probe conformational changes.



2018 ◽  
Vol 6 (3) ◽  
pp. 452-455 ◽  
Author(s):  
Muhit Rana ◽  
Erik E. Augspurger ◽  
Mustafa Salih Hizir ◽  
Esma Alp ◽  
Mehmet V. Yigit

Hybridization chain reaction is programmed for DNA-based molecular logic gate operations using specific initiators and metal ion combinations.



2018 ◽  
Vol 42 (24) ◽  
pp. 20180-20189 ◽  
Author(s):  
Jinping Song ◽  
Qi Ma ◽  
Sufang Zhang ◽  
Huijun Liu ◽  
Yong Guo ◽  
...  

S,N-CNPs with high quantum yield exhibited potential multiple applications including metal ion detection, IMP logic gate fabrication and bioimaging.



2019 ◽  
Vol 72 (3) ◽  
pp. 206 ◽  
Author(s):  
Wenxia Liu ◽  
Zhiguang Suo ◽  
Yihao Liu ◽  
Lingyan Feng ◽  
Binbin Zhang ◽  
...  

In this paper, we report a synthesised water soluble perylene derivative, N,N′-di(2-aspartic acid)-perylene-3,4,9,10-tetracarboxylic diimide (PASP), for highly sensitive and repeatable detection of copper (Cu2+) and aluminium ions (Al3+) and novel logic gate operation. In the presence of metal ions, a dramatic decrease in PASP optical intensity was induced based on the strong interaction between terminal carboxy groups and the metal ions. Detection limits of 0.22 and 0.24μM were respectively obtained at physiological pH. The signals could be recovered upon the addition of ethylenediaminetetraacetate (EDTA) and P2O74−, which competed for Cu2+ and Al3+ in the PASP-CuII and PASP-AlIII systems and induced their dissociation as secondary sensors for anions. At least four detection cycles were performed with a high recovery efficiency. Based on these phenomena, a novel three-level logic gate (OR-IMP-OR) was performed for smart signal readout with metal ions (Cu2+ and Al3+) and anions (EDTA and P2O74−) as input signals, and the relative change of optical intensity of PASP as output signal. Furthermore, the prepared PASP molecule also responded sensitively to Cu2+ and Al3+ in 10% diluted serum medium.



Author(s):  
R. Levi-Setti ◽  
J. M. Chabala ◽  
Y. L. Wang

We have shown the feasibility of 20 nm lateral resolution in both topographic and elemental imaging using probes of this size from a liquid metal ion source (LMIS) scanning ion microprobe (SIM). This performance, which approaches the intrinsic resolution limits of secondary ion mass spectrometry (SIMS), was attained by limiting the size of the beam defining aperture (5μm) to subtend a semiangle at the source of 0.16 mr. The ensuing probe current, in our chromatic-aberration limited optical system, was 1.6 pA with Ga+ or In+ sources. Although unique applications of such low current probes have been demonstrated,) the stringent alignment requirements which they imposed made their routine use impractical. For instance, the occasional tendency of the LMIS to shift its emission pattern caused severe misalignment problems.



Author(s):  
R. Levi-Setti ◽  
J.M. Chabala ◽  
Y.L. Wang

Finely focused beams extracted from liquid metal ion sources (LMIS) provide a wealth of secondary signals which can be exploited to create high resolution images by the scanning method. The images of scanning ion microscopy (SIM) encompass a variety of contrast mechanisms which we classify into two broad categories: a) Emission contrast and b) Analytical contrast.Emission contrast refers to those mechanisms inherent to the emission of secondaries by solids under ion bombardment. The contrast-carrying signals consist of ion-induced secondary electrons (ISE) and secondary ions (ISI). Both signals exhibit i) topographic emission contrast due to the existence of differential geometric emission and collection effects, ii) crystallographic emission contrast, due to primary ion channeling phenomena and differential oxidation of crystalline surfaces, iii) chemical emission or Z-contrast, related to the dependence of the secondary emission yields on the Z and surface chemical state of the target.



Author(s):  
J. A. N. Zasadzinski ◽  
R. K. Prud'homme

The rheological and mechanical properties of crosslinked polymer gels arise from the structure of the gel network. In turn, the structure of the gel network results from: thermodynamically determined interactions between the polymer chain segments, the interactions of the crosslinking metal ion with the polymer, and the deformation history of the network. Interpretations of mechanical and rheological measurements on polymer gels invariably begin with a conceptual model of,the microstructure of the gel network derived from polymer kinetic theory. In the present work, we use freeze-etch replication TEM to image the polymer network morphology of titanium crosslinked hydroxypropyl guars in an attempt to directly relate macroscopic phenomena with network structure.



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