scholarly journals Towards the systematic design of multilayer O/W emulsions with tannic acid as an interfacial antioxidant

RSC Advances ◽  
2021 ◽  
Vol 11 (38) ◽  
pp. 23616-23626
Author(s):  
Savvia Alexandraki ◽  
Epameinondas Leontidis
Keyword(s):  
Tannic Acid ◽  
Systematic Design ◽  
Step Method ◽  
Lbl Films

Three-step method optimizes multilayer emulsion for maximum tannic acid (TA) amount at surfaces. (1) TA–emulsifier bulk interactions assessed. (2) LbL films built for optimal TA presence. (3) Emulsions built as per LbL design and TA action evaluated.

scholarly journals Single-Step Self-Assembly and Physical Crosslinking of PEGylated Chitosan Nanoparticles by Tannic Acid

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 749 ◽  
Author(s):  
Raven A. Smith ◽  
Rebecca C. Walker ◽  
Shani L. Levit ◽  
Christina Tang
Keyword(s):  
Tannic Acid ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Hydrophobic Interactions ◽  
Chitosan Nanoparticles ◽  
Single Step ◽  
Step Method ◽  
Core Mass ◽  
Solids Concentration ◽  
Formulation Parameters

Chitosan-based nanoparticles are promising materials for potential biomedical applications. We used Flash NanoPrecipitation as a rapid, scalable, single-step method to achieve self-assembly of crosslinked chitosan nanoparticles. Self-assembly was driven by electrostatic interactions, hydrogen bonding, and hydrophobic interactions; tannic acid served to precipitate chitosan to seed nanoparticle formation and crosslink the chitosan to stabilize the resulting particles. The size of the nanoparticles can be tuned by varying formulation parameters including the total solids concentration and block copolymer to core mass ratio. We demonstrated that hydrophobic moieties can be incorporated into the nanoparticle using a lipophilic fluorescent dye as a model system.

Green Fabrication of Hydrogel-Immobilized Au@Ag Nanoparticles Using Tannic Acid and Application in Catalysis

2021 ◽  
Author(s):  
Hengxi He ◽  
Didier Astruc ◽  
Haibin Gu
Keyword(s):  
Tannic Acid ◽  
Ag Nanoparticles ◽  
Core Shell ◽  
Step Method ◽  
Shell Nanoparticles ◽  
Active Core ◽  
Catalytically Active ◽  
One Step ◽  
Core Shell Nanoparticles

Highly catalytically active core-shell nanoparticles (NPs) have attracted great attention towards applications. Herein, core-shell Au@AgNPs were simply obtained via a green and cheap one-step method using tannic acid (TA) as...

scholarly journals Determining the effect of natural inhibitors on sesame meal degradability using in vitro three step method

2021 ◽  
Vol 91 (5) ◽  
pp. 513-521
Author(s):  
Maghsoud Besharati ◽  
◽  
Valiollah Palangi ◽  
Akbar Taghizadeh ◽  
Adem Kaya ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Essential Oil ◽  
Tannic Acid ◽  
Crude Protein ◽  
Neutral Detergent Fiber ◽  
Step Method ◽  
Sesame Meal ◽  
Cinnamon Essential Oil ◽  
Natural Inhibitors

The aim of this experiment was to investigate the beneficial effect of monensin, tannic acid and cinnamon essential oil addition on sesame meal degradability by the three-step in vitro method. The effect of experimental additives on the degradability of sesame meal in the rumen, after rumen and in the whole gastrointestinal tract was significant (P<0.05). The in vitro ruminal and intestinal digestibility of sesame meal crude protein with experimental additives was in the range of 76 to 84% and 49 to 60%, respectively. The intestinal degradability of crude protein increased with the addition of cinnamon essential oil (about 10%). Addition of monensin, tannic acid, and cinnamon essential oil significantly increased the degradability of Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF) in the rumen, intestines and the whole gastrointestinal tract. The results showed that cinnamon essential oil (125 mg/L) increased the degradability of dry matter (DM), organic matter (OM), crude protein (CP), ADF and NDF in the rumen, after rumen and the whole digestive tract compared to all treatments (P<0.05). The results showed that addition of tannic acid (100 mg/L) decreased the disappearance of crude protein in the rumen, while it increased crude protein’s disappearance in the after rumen (P<0.05).

A New Approach to the Demonstration of Oxidase-Localization Using Tannic Acid Or Catechin

1973 ◽  
Vol 31 ◽  
pp. 698-699
Author(s):  
V. Mizuhira ◽  
Y. Futaesaku
Keyword(s):  
Cross Section ◽  
Tannic Acid ◽  
Elastic Fiber ◽  
The Other ◽  
New Approach ◽  
Tissue Block ◽  
Active Reaction ◽  
The Cross

Previously we reported that tannic acid is a very effective fixative for proteins including polypeptides. Especially, in the cross section of microtubules, thirteen submits in A-tubule and eleven in B-tubule could be observed very clearly. An elastic fiber could be demonstrated very clearly, as an electron opaque, homogeneous fiber. However, tannic acid did not penetrate into the deep portion of the tissue-block. So we tried Catechin. This shows almost the same chemical natures as that of proteins, as tannic acid. Moreover, we thought that catechin should have two active-reaction sites, one is phenol,and the other is catechole. Catechole site should react with osmium, to make Os- black. Phenol-site should react with peroxidase existing perhydroxide.

Structural preservation and absolute contrast of catalase crystal sections prepared with tannic acid

1983 ◽  
Vol 41 ◽  
pp. 448-449
Author(s):  
C.W. Akey ◽  
M. Szalay ◽  
S.J. Edelstein
Keyword(s):  
Tannic Acid ◽  
Beef Heart ◽  
X Rays ◽  
Screw Axis ◽  
General Applicability ◽  
Thin Sections ◽  
Beef Liver ◽  
3 Dimensional ◽  
Dimensional Reconstruction ◽  
Structural Preservation

Three methods of obtaining 20 Å resolution in sectioned protein crystals have recently been described. They include tannic acid fixation, low temperature embedding and grid sectioning. To be useful for 3-dimensional reconstruction thin sections must possess suitable resolution, structural fidelity and a known contrast. Tannic acid fixation appears to satisfy the above criteria based on studies of crystals of Pseudomonas cytochrome oxidase, orthorhombic beef liver catalase and beef heart F1-ATPase. In order to develop methods with general applicability, we have concentrated our efforts on a trigonal modification of catalase which routinely demonstrated a resolution of 40 Å. The catalase system is particularly useful since a comparison with the structure recently solved with x-rays will permit evaluation of the accuracy of 3-D reconstructions of sectioned crystals.Initially, we re-evaluated the packing of trigonal catalase crystals studied by Longley. Images of the (001) plane are of particular interest since they give a projection down the 31-screw axis in space group P3121. Images obtained by the method of Longley or by tannic acid fixation are negatively contrasted since control experiments with orthorhombic catalase plates yield negatively stained specimens with conditions used for the larger trigonal crystals.

Cell Fine Structure as Revealed in Dessicator-Dried Resinless Sections

1981 ◽  
Vol 39 ◽  
pp. 622-623
Author(s):  
R. P. Becker ◽  
J. J. Wolosewick ◽  
J. Ross-Stanton
Keyword(s):  
Fine Structure ◽  
Tannic Acid ◽  
Three Dimensional ◽  
Albino Rats ◽  
Cell Organelles ◽  
Vascular Perfusion ◽  
Thin Sections ◽  
Carbon Coated ◽  
Embedding Medium ◽  
Polyethylene Glycol 6000

Methodology has been introduced recently which allows transmission and scanning electron microscopy of cell fine structure in semi-thin sections unencumbered by an embedding medium. Images obtained from these “resinless” sections show a three-dimensional lattice of microtrabeculfee contiguous with cytoskeletal structures and membrane-bounded cell organelles. Visualization of these structures, especially of the matiiDra-nous components, can be facilitated by employing tannic acid in the fixation step and dessicator drying, as reported here.Albino rats were fixed by vascular perfusion with 2% glutaraldehyde or 1.5% depolymerized paraformaldehyde plus 2.5% glutaraldehyde in 0.1M sodium cacodylate (pH 7.4). Tissues were removed and minced in the fixative and stored overnight in fixative containing 4% tannic acid. The tissues were rinsed in buffer (0.2M cacodylate), exposed to 1% buffered osmium tetroxide, dehydrated in ethyl alcohol, and embedded in pure polyethylene glycol-6000 (PEG). Sections were cut on glass knives with a Sorvall MT-1 microtome and mounted onto poly-L-lysine, formvar-carbon coated grids while submerged in a solution of 95% ethanol containing 5% PEG.

Extracellular filaments in the perineurium of the florida lobster, Panulirus argus

1987 ◽  
Vol 45 ◽  
pp. 784-785
Author(s):  
Roy J. Baerwald ◽  
Lura C. Williamson
Keyword(s):  
Blood Brain Barrier ◽  
Glial Cells ◽  
Tannic Acid ◽  
Nerve Cord ◽  
Ventral Nerve Cord ◽  
Florida Keys ◽  
Panulirus Argus ◽  
Brain Barrier ◽  
Cacodylate Buffer ◽  
Nerve Cords

In arthropods the perineurium surrounds the neuropile, consists of modified glial cells, and is the morphological basis for the blood-brain barrier. The perineurium is surrounded by an acellular neural lamella, sometimes containing scattered collagen-like fibrils. This perineurial-neural lamellar complex is thought to occur ubiquitously throughout the arthropods. This report describes a SEM and TEM study of the sheath surrounding the ventral nerve cord of Panulirus argus.Juvenile P. argus were collected from the Florida Keys and maintained in marine aquaria. Nerve cords were fixed for TEM in Karnovsky's fixative and saturated tannic acid in 0.1 M Na-cacodylate buffer, pH = 7.4; post-fixed in 1.0% OsO4 in the same buffer; dehydrated through a graded series of ethanols; embedded in Epon-Araldite; and examined in a Philips 200 TEM. Nerve cords were fixed for SEM in a similar manner except that tannic acid was not used.

Purification of Urokinase from Complex Mixtures Using Immobilized Monoclonal Antibody Against Urokinase Light Chain

1983 ◽  
Vol 49 (01) ◽  
pp. 024-027 ◽  
Author(s):  
David Vetterlein ◽  
Gary J Calton
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Culture Media ◽  
Biological Fluids ◽  
Single Step ◽  
High Yield ◽  
Step Method ◽  
Commercial Preparation ◽  
E Coli ◽  
Tissue Culture Media

SummaryThe preparation of a monoclonal antibody (MAB) against high molecular weight (HMW) urokinase light chain (20,000 Mr) is described. This MAB was immobilized and the resulting immunosorbent was used to isolate urokinase starting with an impure commercial preparation, fresh urine, spent tissue culture media, or E. coli broth without preliminary dialysis or concentration steps. Monospecific antibodies appear to provide a rapid single step method of purifying urokinase, in high yield, from a variety of biological fluids.

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