scholarly journals Improvement of cytotoxicity and necrosis activity of ganoderic acid a through the development of PMBN-A.Her2-GA as a targeted nano system

RSC Advances ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 1228-1237
Author(s):  
P. Motamed Fath ◽  
M. Rahimnejad ◽  
S. Moradi-kalbolandi ◽  
B. Ebrahimi Hosseinzadeh ◽  
T. Jamshidnejad-tosaramandani
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Ganoderic Acid ◽  
First Time ◽  
Natural Agent

In this article, GA-A is used for the first time as a natural agent for targeting breast cancer cells based on the newly developed nano carrier as a targeted DDS.

scholarly journals Breast Cancer Cell Re-Dissemination from Lung Metastases—A Mechanism for Enhancing Metastatic Burden

2021 ◽  
Vol 10 (11) ◽  
pp. 2340
Author(s):  
Lucia Borriello ◽  
John Condeelis ◽  
David Entenberg ◽  
Maja H. Oktay
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Metastatic Disease ◽  
Breast Cancer Cells ◽  
Lung Metastases ◽  
Primary Tumors ◽  
Metastatic Burden ◽  
First Time ◽  
Do So

Although metastatic disease is the primary cause of mortality in cancer patients, the mechanisms leading to overwhelming metastatic burden are still incompletely understood. Metastases are the endpoint of a series of multi-step events involving cancer cell intravasation, dissemination to distant organs, and outgrowth to metastatic colonies. Here we show, for the first-time, that breast cancer cells do not solely disseminate to distant organs from primary tumors and metastatic nodules in the lymph nodes, but also do so from lung metastases. Thus, our findings indicate that metastatic dissemination could continue even after the removal of the primary tumor. Provided that the re-disseminated cancer cells initiate growth upon arrival to distant sites, cancer cell re-dissemination from metastatic foci could be one of the crucial mechanisms leading to overt metastases and patient demise. Therefore, the development of new therapeutic strategies to block cancer cell re-dissemination would be crucial to improving survival of patients with metastatic disease.

Targeted photoresponsive TiO2–coumarin nanoconjugate for efficient combination therapy in MDA-MB-231 breast cancer cells: synergic effect of photodynamic therapy (PDT) and anticancer drug chlorambucil

MedChemComm ◽  
2015 ◽  
Vol 6 (5) ◽  
pp. 769-777 ◽  
Author(s):  
Moumita Gangopadhyay ◽  
Sourav K. Mukhopadhyay ◽  
S. Karthik ◽  
Shrabani Barman ◽  
N. D. Pradeep Singh
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Combination Therapy ◽  
Cancer Cells ◽  
Anticancer Drug ◽  
Breast Cancer Cells ◽  
Single Component ◽  
Synergic Effect ◽  
Nanoparticle System ◽  
First Time

We have developed for the first time an excellent targeted metallic single component nanoparticle system for combination of PDT and chemotherapy.

scholarly journals Immunocytochemical and autoradiographic demonstration of prolactin binding to human breast cancer cells in tissue culture.

1982 ◽  
Vol 30 (2) ◽  
pp. 153-156 ◽  
Author(s):  
J A Paterson ◽  
H Salih ◽  
R P Shiu
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Specific Binding ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Human Prolactin ◽  
First Time ◽  
Silver Grains

Specific binding of human prolactin to human breast cancer cells in culture was demonstrated for the first time by immunocytochemistry ad autoradiography. Using the former technique, it was found that prolactin binding to the cell surface was heterogeneous: some cells showing intense to moderate peroxidase reaction product and some cells showing absence of reaction product. After the cells were incubated with 125I-labeled prolactin and then embedded in plastic, autoradiography of sections revealed both surface-localized and intracellular silver grains, the latter suggesting internalization of prolactin. These two cytological techniques are useful for visualizing prolactin binding to human breast cancer cells and are applicable to studies on prolactin binding in human breast cancer.

Periplaneta Americana Extract Inhibits Proliferation and Motility of Human Breast Cancer Cells Through MAPK/ERK Signaling Pathway and Promotes Cell Apoptosis

2020 ◽  
Vol 19 (2) ◽  
pp. 194-198
Author(s):  
Xiaoying Wu ◽  
Zhe Sun ◽  
Bailing Shi ◽  
Xiaoli Zhang
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Breast Cancer Cells ◽  
Periplaneta Americana ◽  
Erk Signaling ◽  
Antitumor Activities ◽  
Cancer Multiple ◽  
First Time ◽  
Mcf 7

Breast cancer is the most common malignant tumor among women, and its incidence is second only to cervical cancer in China. The search for natural products with antitumor activity is a potentially appealing strategy for the treatment of breast cancer. Multiple studies have shown that extracts of Periplaneta americana exhibit antitumor activities. However, there is no study on the effect of P. americana extracts on breast cancer. We have shown here for the first time that the extracts of P. americana inhibit survival of MCF-7 breast cancer cells via interference with MAPK/ERK signaling pathway.

Induction of apoptosis in MDA-MB-231 breast cancer cells by a PARP1-targeting PROTAC small molecule

2019 ◽  
Vol 55 (3) ◽  
pp. 369-372 ◽  
Author(s):  
Qiuye Zhao ◽  
Tianlong Lan ◽  
Shang Su ◽  
Yu Rao
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Small Molecule ◽  
Breast Cancer Cells ◽  
Induction Of Apoptosis ◽  
First Time

We report for the first time a PARP1-targeting PROTAC small molecule to selectively induce the cleavage of PARP1.

scholarly journals Knockdown Of Udp-Glucose Dehydrogenase Facilitates Epirubicin-Resistance In MDA-MB-231 Breast Cancer Cells By Regulation Of Hyaluronan Synthesis.

2020 ◽  
Author(s):  
Daiana L. Vitale ◽  
Ilaria Caon ◽  
Arianna Parnigoni ◽  
Ina Sevic ◽  
Fiorella M. Spinelli ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Glucose Dehydrogenase ◽  
Sugar Metabolism ◽  
Tumor Resistance ◽  
Ha Synthase ◽  
Cytotoxicity Effects ◽  
First Time

Abstract Background: The catalytic action of the UDP-glucose transferase dehydrogenase (UGDH) enzyme produces UDP-glucuronic acid (UDP-GlcUA). The main detoxifying pathway of Epirubicin (EPI) is via glucuronidation by the specific transferase UGT2B7 (UDP-Glucuronosyltransferase-2B7), adding UDP-GlcUA to generates 4´-O-b-D-glucuronyl-4´-epi-doxorubicin. UDP-GlcUA is also a precursor of several glycosaminoglycans (GAGs) like hyaluronan (HA). Therefore, the EPI detoxifying pathway might be associated with GAGs metabolism, related to drug deactivation and tumor resistance. This work aimed to evaluate the effect of knockdown on the UGDH gene on EPI response and HA metabolism in the aggressive breast cancer cells MDA-MB 231. Methods: MDA-MB-231 cells were transfected in vitro with UGDH-specific siRNA for UGDH knockdown and treated with EPI. Viability, apoptosis, and cytotoxicity effects were evaluated. EPI intracellular accumulation was analyzed by flow cytometry. Differences in extracellular matrix (ECM) were analyzed through a particle exclusion assay and the composition of HA using hyaluronidase. The soluble HA secreted was detected by an ELISA like assay. Besides, gene expression of HA synthase and hyaluronidase (HYAL) enzymes were analyzed by RT-qPCR. To analyze the effect of UGDH knockdown and EPI treatment on autophagy, we evaluated the expression of the autophagosome marker, LC3-II by RT-qPCR and western blot, and its subcellular localization by confocal microscopy. Results: EPI accumulation increased during UGDH knockdown, but it was observed a decrease in cell death. HA synthesis and HA coated around the cells increased. In turn, it was found up-regulation of the expression of HYALs. Within the mechanisms activated by tumor cells to avoid EPI activity, an increase in autophagy was detected. Conclusions: for the first time we show that an increase in the expression, deposition and catabolism of HA positively contributed to the development of a resistant phenotype in breast cancer cells by a mechanism associated to sugar metabolism, specifically of UDP-GlcUA .

scholarly journals Let-7a induces metabolic reprogramming in breast cancer cells via targeting mitochondrial encoded ND4

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Praveen Sharma ◽  
Vibhuti Sharma ◽  
Tarunveer Singh Ahluwalia ◽  
Nilambra Dogra ◽  
Santosh Kumar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mitochondrial Genome ◽  
Cancer Cells ◽  
Transcriptional Repression ◽  
Breast Cancer Cells ◽  
Metabolic Reprogramming ◽  
Mitochondrial Transcription ◽  
A Cell ◽  
First Time

Abstract Background and objectives MicroRNA (miRNA) that translocate from the nucleus to mitochondria are referred to as mitochondrial microRNA (mitomiR). Albeit mitomiRs have been shown to modulate gene expression, their functional impact within mitochondria is unknown. The main objective of this study is to investigate whether the mitochondrial genome is regulated by miR present inside the mitochondria. Methods and results Here, we report mitomiR let-7a regulates mitochondrial transcription in breast cancer cells and reprogram the metabolism accordingly. These effects were mediated through the interaction of let-7a with mtDNA, as studied by RNA pull-down assays, altering the activity of Complex I in a cell line-specific manner. Our study, for the first time, identifies the role of mitomiR (let-7a) in regulating the mitochondrial genome by transcriptional repression and its contribution to regulating mitochondrial metabolism of breast cancer cells. Conclusion These findings uncover a novel mechanism by which mitomiR regulates mitochondrial transcription.

A recyclable chitosan-based QCM biosensor for sensitive and selective detection of breast cancer cells in real time

The Analyst ◽  
2014 ◽  
Vol 139 (23) ◽  
pp. 6259-6265 ◽  
Author(s):  
Shaolian Zhang ◽  
Haihua Bai ◽  
Jinmei Luo ◽  
Peihui Yang ◽  
Jiye Cai
Keyword(s):  
Breast Cancer ◽  
Folic Acid ◽  
Cancer Cells ◽  
Real Time ◽  
Breast Cancer Cells ◽  
Time Measurement ◽  
Selective Detection ◽  
Real Time Measurement ◽  
First Time ◽  
Mcf 7

A sensitive and recyclable QCM biosensor for the real-time measurement of MCF-7 breast cancer cells was developed for the first time using folic acid coupled to chitosan as an excellent biocompatible biosensor film.

scholarly journals Flavanoids fromthe Stembark of Chisocheton pentandrus(Meliaceae)

2017 ◽  
pp. 122-126
Author(s):  
Supriatno Supriatno ◽  
Ace Tatang Hidayat ◽  
Kindi Farabi ◽  
Fajar Fauzi Abdullah ◽  
Nurlelasari Nurlelasari ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Spectral Data ◽  
Breast Cancer Cells ◽  
Chemical Structure ◽  
First Time ◽  
Mcf 7

Two flavanoid compounds, catechin (1) and epicatechin (2), have been isolated from the stembark of Chisocheton pentandrus. The chemical structure of compounds1and2were identified byspectroscopic data including, UV, IR, NMR (1H, 13C, DEPT 135°, HMQC, HMBC, 1H-1H COSY) and MS and by comparing with previously reported spectral data. Compounds 1 and 2, were isolated in this plant for first time and showed no cytotoxic activity against MCF-7 breast cancer cells.

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