scholarly journals Expression of Concern: Stromal interaction molecule 1 (STIM1) regulates growth, cell cycle and apoptosis of human tongue squamous carcinoma cells

2021 ◽  
Vol 41 (4) ◽  
Keyword(s):  
Cell Cycle ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Stromal Interaction Molecule 1 ◽  
Squamous Carcinoma Cells ◽  
Human Tongue ◽  
Tongue Squamous Carcinoma

scholarly journals Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Cuilan Hou ◽  
Wenguang Li ◽  
Zengyou Li ◽  
Jing Gao ◽  
Zhenjie Chen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Fluorescence Microscopy ◽  
Gelatin Zymography ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Migration And Invasion ◽  
Antioxidant Mechanism ◽  
Squamous Carcinoma Cells ◽  
Human Tongue ◽  
Tongue Squamous Carcinoma

Isoliquiritigenin (ISL), a natural antioxidant, has antitumor activity in different types of cancer cells. However the antitumor effect of ISL on human tongue squamous carcinoma cells (TSCC) is not clear. Here we aimed to investigate the effects of synthetic isoliquiritigenin (S-ISL) on TSCC and elucidate the underlying mechanisms. S-ISL was synthesized and elucidated from its nuclear magnetic resonance spectrum and examined using high performance liquid chromatography. The effects of S-ISL on TSCC cells (Tca8113) were evaluated in relation to cell proliferation, apoptosis and adhesion, migration, and invasion using sulforhodamine B assay, fluorescence microscopy technique, flow cytometry (FCM) analysis, and Boyden chamber assay. The associated regulatory mechanisms were examined using FCM and fluorescence microscopy for intracellular reactive oxygen species (ROS) generation, Gelatin zymography assay for matrix metalloproteinase (MMP) activities, and Western blot for apoptosis regulatory proteins (Bcl-2 and Bax). Our data indicated that S-ISL inhibited Tca8113 cell proliferation, adhesion, migration, and invasion while promoting the cell apoptosis. Such effects were accompanied by downregulation of Bcl-2 and upregulation of Bax, reduction of MMP-2 and MMP-9 activities, and decreased ROS production. We conclude that S-ISL is a promising agent targeting TSCC through multiple anticancer effects, regulated by its antioxidant mechanism.

scholarly journals Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Xiaobo Cui ◽  
Laixiao Song ◽  
Yunfei Bai ◽  
Yaping Wang ◽  
Boqian Wang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transmembrane Protein ◽  
Squamous Carcinoma ◽  
Stromal Interaction Molecule 1 ◽  
Mapk Pathways ◽  
Squamous Carcinoma Cells ◽  
Human Tongue ◽  
M Phase ◽  
Cellular Apoptosis ◽  
Colony Number

Oral tongue squamous cell carcinoma (OTSCC) is the most common type of oral carcinomas. However, the molecular mechanism by which OTSCC developed is not fully identified. Stromal interaction molecule 1 (STIM1) is a transmembrane protein, mainly located in the endoplasmic reticulum (ER). STIM1 is involved in several types of cancers. Here, we report that STIM1 contributes to the development of human OTSCC. We knocked down STIM1 in OTSCC cell line Tca-8113 with lentivirus-mediated shRNA and found that STIM1 knockdown repressed the proliferation of Tca-8113 cells. In addition, we also showed that STIM1 deficiency reduced colony number of Tca-8113 cells. Knockdown of STIM1 repressed cells to enter M phase of cell cycle and induced cellular apoptosis. Furthermore, we performed microarray and bioinformatics analysis and found that STIM1 was associated with p53 and MAPK pathways, which may contribute to the effects of STIM1 on cell growth, cell cycle, and apoptosis. Finally, we confirmed that STIM1 controlled the expression of MDM2, cyclin-dependent kinase 4 (CDK4), and growth arrest and DNA damage inducible α (GADD45A) in OTSCC cells. In conclusion, we provide evidence that STIM1 contributes to the development of OTSCC partially through regulating p53 and MAPK pathways to promote cell cycle and survival.

scholarly journals Electrochemical Assessment of Anticancer Compounds on the Human Tongue Squamous Carcinoma Cells

Sensors ◽  
2020 ◽  
Vol 20 (9) ◽  
pp. 2632 ◽  
Author(s):  
Chun-Chung Huang ◽  
Tse-Hua Tung ◽  
Chien-Chu Huang ◽  
Shao-Yi Lin ◽  
Shih-Chi Chao ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Squamous Carcinoma ◽  
Annexin V ◽  
Time Dependent ◽  
Second Primary ◽  
Second Primary Malignancy ◽  
Primary Malignancy ◽  
Squamous Carcinoma Cells ◽  
Human Tongue ◽  
Tongue Squamous Carcinoma

The most common oral cancer is squamous cell carcinoma (SCC) and its highest occurrence is in the tongue. Almost 30% of patients with one primary head and neck tumor will have a second primary malignancy. In recent studies, two novel plant extracts, andrographolide and cannabidiol (CBD), have been exploited for their anticancer effects. Here, we investigated the cytotoxic effects of these two compounds on SCC-25 cells, a human tongue squamous carcinoma cell line, and compared the outcomes with two chemotherapeutic drugs, cisplatin and fluorouracil. Electric cell substrate impedance sensing (ECIS) system was applied to measure frequency- and time-dependent impedance of SCC-25 cell-covered electrodes and to further assess subtle changes in cell morphology and micromotion in response to different concentrations (0, 10, 30, 100, and 300 µM) of these compounds. AlamarBlue and Annexin V/7-AAD binding assays were used to measure the concentration dependent changes in viability and apoptosis of SCC-25 cells. Our results demonstrate that 24 hours after exposure to 30 µM CBD can significantly decrease the micromotion rate, damage the integrity of cell morphology, reduce cell viability, and induce higher apoptosis in treated SCC-25 cells, while the other three drugs attain similar effects at the concentration of 100 µM or higher. The apoptosis-induced changes in cell morphology and micromotion monitored by ECIS correlate well with biochemical assays. Thus, both frequency- and time-dependent impedance measurements using ECIS can be used to real-time follow cancer cell activities in response to anticancer drugs with different temporal cytotoxicity profiles.

Scutellariae radixInduces Apoptosis in Chemoresistant SCC-25 Human Tongue Squamous Carcinoma Cells

2015 ◽  
Vol 43 (01) ◽  
pp. 167-181 ◽  
Author(s):  
Byul-Bora Choi ◽  
Jeong Hae Choi ◽  
Sang-Rye Park ◽  
Ji-Young Kim ◽  
Jin-Woo Hong ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Morphology ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Mitogen Activated Protein Kinase ◽  
Dependent Manner ◽  
Scutellariae Radix ◽  
Squamous Carcinoma Cells ◽  
Human Tongue ◽  
Oral Squamous Carcinoma

Scutellariae radix is one of the most widely used anticancer herbal medicines in several Asian countries, including Korea, Japan, and China. Squamous cell carcinoma (SCC) is one of the most common head and neck carcinomas, which is highly invasive and metastatic, and can potentially develop chemoresistance. Therefore, new effective treatment methods are urgently needed. We determined the effects of Scutellariae radix on SCC-25 cells using the WST-1 assay, F-actin staining, flow cytometry analysis, immunofluorescence staining, and western blot analysis. Scutellariae radix treatment inhibited SCC-25 cell growth in a dose- and time-dependent manner, but it did not inhibit HaCaT (human keratinocyte) cell growth. Changes in cell morphology and disruption of filamentous (F)-actin organization were observed. Scutellariae radix-induced apoptosis as indicated by the translocation of cytochrome c and apoptosis-inducing factor (AIF) into the nucleus and cytosol. Scutellariae radix-induced an increase in cells with sub-G1 DNA content, and increased Bax, cleaved caspase-3, caspase-7, caspase-9, DNA fragmentation factor 45 (DFF 45), and poly(ADP-ribose) polymerase-1 (PARP-1) expression levels. Furthermore, increased expression of phosphorylated mitogen-activated protein kinase (MAPK)-related proteins was detected. The antitumor effect of Scutellariae radix was due to decreased cell proliferation, changes in cell morphology, and the activation of caspase and MAPK pathways. Taken together, the findings of this study highlight the anticancer activity of Scutellariae radix in chemoresistant SCC-25 oral squamous carcinoma cells.

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