GLC analysis of Indian rapeseed-mustard to study the variability of fatty acid composition

2000 ◽  
Vol 28 (6) ◽  
pp. 581-582 ◽  
Author(s):  
N. Kaushik ◽  
A. Agnihotri

Rapeseed-mustard is one of the most economically important oilseed crops in India. Speciality oils having high amounts of a specific fatty acid are of immense importance for both nutritional and industrial purposes. Oil high in oleic acid has demand in commercial food-service applications due to a long shelf-life and cholesterol-reducing properties. Both linoleic and linolenic acids are essential fatty acids; however, less than 3% linolenic acid is preferred for oil stability. High erucic acid content is beneficial for the polymer industry, whereas low erucic acid is recommended for food purposes. Therefore, it is important to undertake systematic characterization of the available gene pool for its variable fatty acid profile to be utilized for specific purposes. In the present study the Indian rapeseed-mustard germplasm and some newly developed low-erucic-acid strains were analysed by GLC to study the fatty acid composition in these lines. The GLC analysis revealed that the rapeseed-mustard varieties being commonly grown in India are characterized by high erucic acid content (30–51%) in the oil with low levels of oleic acid (13–23%). However, from among the recently developed low-erucic-acid strains, several lines were identified with comparatively high oleic acid (60–70%), moderate to high linoleic acid (13–40%) and low linolenic acid (< 10%) contents. Work is in progress at TERI (New Delhi, India) to utilize these lines for development of strains with particular fatty acid compositions for specific purposes.

1975 ◽  
Vol 55 (1) ◽  
pp. 197-203 ◽  
Author(s):  
G. RAKOW ◽  
D. I. McGREGOR

Fatty acid composition and oil formation were followed in developing seeds of two "linolenic acid lines" of low erucic acid rapeseed (Brassica napus L.) containing above and below normal linolenic acid levels to determine how these different levels of linolenic acid are derived. Under controlled growth room conditions, patterns of fresh weight and dry weight accumulation showed that seeds of both lines matured over the same period of time. During this time, both lines accumulated equivalent amounts of oil. The fatty acid composition of the oils of mature seed of both lines were similar for palmitic (16:0), palmitoleic (16:1), stearic (18:0), linoleic (18:2) and eicosenoic acid (20:1) when expressed either on a percent basis or a weight basis. However, oleic (18:1) and linolenic (18:3) acid were reciprocally related on a percent basis. The seeds of the two lines accumulated different weights of oleic and linolenic acid. Since the accumulation occurred over the same length of time, the difference in oleic and linolenic acid content was caused by different rates of accumulation. Equivalent chlorophyll contents, obtained for both lines, failed to show a relationship between chlorophyll content (indicative of photosynthetic activity) and linolenic acid accumulation.


Helia ◽  
2015 ◽  
Vol 38 (62) ◽  
Author(s):  
Claudio Ferfuia ◽  
Maurizio Turi ◽  
Gian Paolo Vannozzi

AbstractHigh temperature enhances the oleic acid content in the oil of normal cultivars but conflicting results are reported on temperature effects on oleic acid content in HO cultivars: either no effect or an increase in oleic acid content with temperature. To investigate the effects of temperature on HO genotypes under natural field conditions, a three-year field trial was conducted using two sowing dates and three HO genotypes (two inbred lines and one hybrid). To compare our results with previous works, growing degree-days (GDD) were computed (base temperature=6°C). GDD accumulated during the “flowering – 25 days after flowering” period influenced fatty acid composition of seed. Oleic and linoleic acid contents were affected by accumulated GDD in two HO genotypes (one inbred line and the hybrid). There was an increase of about 3% in oleic acid content as response to more high GDD accumulated. Their content was not modified by GDD in the other inbred line. There was a genotype×environment interaction that we suppose depending on modifier genes. These genetic factors affected oleic acid content. This indicated the importance of breeding targeted to select hybrids with a stable oleic acid content and higher than 90%. Saturated fatty acids (palmitic and stearic) were also influenced by temperature, and there was genetic variability among genotypes.


Author(s):  
Md. Delwar Hossain ◽  
Kamal Uddin Ahmed ◽  
Mst. Farhana Nazneen Chowdhury ◽  
Alak Barman ◽  
Arif Ahmed ◽  
...  

With a view to studying the qualitative features and the variations in fatty acid composition of 6 rapeseed (B. campestris and B. napus) and mustard (B. juncea) varieties, an experiment was conducted. Among these varieties, BARI Sarisha-14 presented the value of 168.4 which was recorded the highest. Both BARI Sarisha-11 and BARI Sarisha-14 was found with the highest iodine value of 39.44; and the highest amount of acid value was recorded from BARI Sarisha-11 (1.867). Gas-liquid chromatographic (GLC) method has been used to determine the composition of essential fatty acid in the seeds of Brassica spp. (L.). From the GLC analysis, it was found that erucic acid, oleic acid, linoleic acid and lenolenic acid were the prime fatty acids in all the varieties. Erucic acid was in the range of 41.11 – 51.28%, oleic acid was the highest both in BARI Sarisha-11 and BARI Sarisha- 13 contained (18.69%), while BARI Sarisha-9 contained the highest amount of the unsaturated linoleic (17.75%)  and linolenic (15.83%) acids. Moreover, palmitic acid, stearic acid and archidic acid were also present in small amount.


1959 ◽  
Vol 39 (4) ◽  
pp. 437-442 ◽  
Author(s):  
B. M. Craig ◽  
L. R. Wetter

The content of C16, C18, C20, C22 fatty acids were measured by gas liquid phase chromatography and linoleic and linolenic acids by spectral analyses on the oil from seven varieties of rapeseed grown at seven stations in Western Canada. Significant differences were found between varieties for all oil properties except the content of C16 acids. The major variation occurred in C18, C22 and linoleic acids with lesser amounts in the C20 and linolenic acids. The varieties Golden, Argentine, Regina II and Swedish are classed as high, Gute and Arlo as intermediate, and Polish as low erucic acid oils.


HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1082D-1082 ◽  
Author(s):  
Kyoung-Shim Cho ◽  
Hyun-Ju Kim ◽  
Jae-Ho Lee ◽  
Jung-Hoon Kang ◽  
Young-Sang Lee

Fatty acid is known as a physiologically active compound, and its composition in rice may affect human health in countries where rice is the major diet. The fatty acid composition in brown rice of 120 Korean native cultivars was determined by one-step extraction/methylation method and GC. The average composition of 9 detectable fatty acids in tested rice cultivars were as followings: myristic acid; 0.6%, palmitic acid; 21.2%, stearic acid; 1.8%, oleic acid; 36.5%, linoleic acid; 36.3%, linolenic acid; 1.7%, arachidic acid; 0.5%, behenic acid; 0.4%, and lignoceric acid; 0.9%. Major fatty acids were palmitic, oleic and linoleic acid, which composed around 94%. The rice cultivar with the highest linolenic acid was cv. Jonajo (2.1%), and cvs. Pochoenjangmebye and Sandudo showed the highest composition of palmitic (23.4%) and oleic acid (44.8%), respectively. Cultivar Pochuenjangmebye exhitibed the highest composition of saturated fatty acid (28.1%), while cvs. Sandudo and Modo showed the highest mono-unsaturated (44.8%) and poly-unsaturated (42.4%) fatty acid composition, respectively. The oleic acid showed negative correlation with palmitic and linoleic acid, while positive correlation between behenic and lignoceric acids was observed.


Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 292 ◽  
Author(s):  
Elena Cartea ◽  
Antonio De Haro-Bailón ◽  
Guillermo Padilla ◽  
Sara Obregón-Cano ◽  
Mercedes del Rio-Celestino ◽  
...  

The seed oil content and the fatty acid composition of a germplasm collection of Brassica napus and Brassica rapa currently grown in Galicia (northwestern Spain) were evaluated in order to identify potentially interesting genotypes and to assess their suitability as oilseed crops for either edible or industrial purposes. The seeds of the B. rapa landraces had higher oil content (mean 47.3%) than those of B. napus (mean 42.8%). The landraces of both species showed a similar fatty acid profile (12% oleic acid, 13% linoleic acid, 8–9% linolenic acid, 8–9% eicosenoic acid, and 50–51% erucic acid). They were very high in erucic acid content, which is nutritionally undesirable in a vegetable oil, and very low in oleic and linoleic acid contents. Therefore, they could be used for industrial purposes but not as edible oil. The erucic acid content ranged from 42% to 54% of the total fatty acid composition with an average value of 50% in the B. napus landraces whereas in B. rapa, it ranged from 43% to 57%, with an average value of 51%. Considering the seed oil and the erucic acid content together, three varieties within the B. napus collection and two varieties within the B. rapa one seem to be the most promising genotypes for industrial purposes.


1961 ◽  
Vol 41 (1) ◽  
pp. 204-210 ◽  
Author(s):  
B. M. Craig

The fatty acid compositions of the oil from 6 varieties of rapeseed grown at 22 stations were determined by gas liquid phase chromatography. Significant differences were found between stations for all fatty acids and between varieties for palmitic, stearic, oleic, linoleic, eicosenoic and erucic acids. The variations for palmitic, stearic, and eicosenoic acids were small, whereas major variations occurred in oleic, erucic and linoleic acids. A correlation coefficient of −0.975 was found between oleic and erucic acids and a prediction equation was determined to calculate oleic acid from the erucic acid content.


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