Mechanisms of mercury bioremediation

2002 ◽  
Vol 30 (4) ◽  
pp. 672-674 ◽  
Author(s):  
A. M. M. Essa ◽  
L. E. Macaskie ◽  
N. L. Brown
Keyword(s):  
Heavy Metals ◽  
Klebsiella Pneumoniae ◽  
Capture Efficiency ◽  
Mercury Pollution ◽  
Resistant Bacteria ◽  
Toxic Heavy Metals ◽  
Metallic Mercury ◽  
Mercury Volatilization ◽  
Mercury Detoxification ◽  
Mercury Resistant Bacteria

Mercury is one of the most toxic heavy metals, and has significant industrial and agricultural uses. These uses have led to severe localized mercury pollution. Mercury volatilization after its reduction to the metallic form by mercury-resistant bacteria has been reported as a mechanism for mercury bioremediation [Brunke, Deckwer, Frischmuth, Horn, Lunsdorf, Rhode, Rohricht, Timmis and Weppen (1993) FEMS Microbiol. Rev. 11, 145–152; von Canstein, Timmis, Deckwer and Wagner-Dobler (1999) Appl. Environ. Microbiol. 65, 5279–5284]. The reduction/volatilization system requires to be studied further, in order to eliminate the escape of the metallic mercury into the environment. Recently we have demonstrated three different mechanisms for mercury detoxification in one organism, Klebsiella pneumoniae M426, which may increase the capture efficiency of mercury.

scholarly journals IDENTIFIKASI BAKTERI RESISTEN MERKURI PADA KARANG GIGI, URIN DAN FESES PADA INDIVIDU KELURAHAN PAKADOODAN KOTA BITUNG

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Maichel Yorgen
Keyword(s):  
Research Result ◽  
Clinical Manifestations ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Resistance Level ◽  
The Individual ◽  
Mercury Detoxification ◽  
Bacteria Resistance ◽  
Mercury Resistant Bacteria ◽  
Phenyl Mercury

Abstract: Mercury is one of kinds of hazardous metals which is highly found in nature and spread in rocklike, ore, soil, water, and air as the inorganic and organic compounds. The metal of mercury is dangerous to human’s life because it can cause the substantially clinical manifestations started from being poisoned, nerve palsy and even cause death. One of attempts to mercury detoxification can be done by using mercury-resistant microorganism such as mercury-resistant bacteria. That would happen if the mercury spread inside the human’s body with small value in a very chronic time can make the bacteria inside the human’s body to be able to adapt even able to reduce mercury so that it becomes harmless. The research aims to identify the kind of bacteria that resists to mercury, in this case, to the kind of mercury HgCl2 and Phenyl Mercury found on tartar, urine, and feses upon the individual in Pakadoodan Village of Bitung City by using the way to check the value of bacteria resistance to mercury, afterwards the Physiology test, Biochemical, and Morphology is done to identify the kind of that bacteria. From the research result done there are 4 genus of bacteria which was successfully identified, those are, Streptococcus sp, E.Coli, Bacillus sp, and Staphylococcus sp. By the classification HgCl2 is the bacteria with the genus of     Bacillus sp which is found in urine, feses and tartar, and Staphylococcus sp which is found also in urine with the resistance level of 40 ppm each and phenyl mercury is the bacteria with the genus of Streptococcus sp, which is found in tartar and E.Coli which is found in feses and urine with the resistance level 20 ppm each. Keywords: mercury, bacteria, mercury-resistant bacteria Abstrak: Merkuri adalah salah satu jenis logam berbahaya yang banyak ditemukan di alam dan tersebar dalam batu - batuan, biji tambang, tanah, air dan udara sebagai senyawa anorganik dan organik. Logam merkuri sangat berbahaya terhadap kehidupan manusia karena dapat memberikan manifestasi klinik yang cukup bermakna mulai dari keracunan, kelumpuhan saraf bahkan dapat meneyebabkan kematian. Salah satu usaha untuk detoksifikasi merkuri dapat dilakukan dengan menggunakan mikroorganisme resisten merkuri seperti bakteri resisten merkuri. Hal itu dapat terjadi jika merkuri yang terpapar dalam tubuh manusia dengan kadar yang kecil dalam waktu yang sangat kronis dapat membuat bakteri dalam tubuh manusia tersebut dapat beradaptasi bahkan dapat mampu meruduksi merkuri tersebut sehingga menjadi tidak berbahaya. Penelitian ini bertujuan untuk mengidentifikasi jenis bakteri yang resisten terhadap merkuri dalam hal ini terhadap jenis merkuri HgCl2 dan fenil merkuri yang terdapat pada karang gigi, urin dan feses pada individu kelurahan Pakadoodan Kota Bitung dengan cara memeriksa kadar resisten bakteri tersebut terhadap merkuri, setelah itu dilakukan uji fisiologi, uji biokimia dan uji morfolgi untuk mengidentifikasi jenis bakteri tersebut.  Dari hasil penelitian yang dilakukan terdapat 4 genus bakteri yang berhasil diidentifikasi yaitu Streptococcus sp, E.coli, Bacillus sp, dan Staphylococcus sp. Dengan klasifikasi HgCl2 adalah bakteri dengan genus Bacillus sp yang terdapat pada urin, feses dan karang gigi, dan Staphylococcus sp yang terdapat juga pada urin dengan tingkat resistensi masing-masing 40 ppm dan fenil merkuri adalah bakteri dengan genus Streptococcus sp, yang terdapat pada karang gigi dan E.coli yang terdapat pada feses dan urin dengan tingkat resistensi masing-masing 20 ppm. Kata kunci: merkuri, bakteri, bakteri resisten merkuri

scholarly journals Uji resistensi bacillus yang diisolasi dari plak gigi terhadap merkuri dan gentamisin

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Valentino Rakasiwi ◽  
Widdhi Bodhi ◽  
Billy J. Kepel
Keyword(s):  
Atomic Number ◽  
Human Body ◽  
Mercury Level ◽  
Cognitive Disorders ◽  
Resistant Bacteria ◽  
Long Period ◽  
High Mercury ◽  
Mercury Detoxification ◽  
Resistant Microorganism ◽  
Mercury Resistant Bacteria

Abstract: Mercury is an element with atomic number (N) of 80 and has 200,59 relative molecule mass (MR = 200,59). People that are regularly exposed by high mercury, such as due to consuming fish that contains mercury, show cognitive disorders. One of the methods of mercury detoxification is by using mercury-resistant microorganism, such as mercury-resistant bacteria. This should occur if low-level mercury in human body mercury in a relatively long period can make the bacteria adapt and reduce the mercury level, therefore, the bacteria are not dangerous anymore. This study aimed to find out whether the bacillus bacteria were resistant to mercury and gentamycin. Bacillus bacteria were incubated in liquid LB media for 24 hours, The results showed that bacteria’s colonies were found at 10 ppm, 20 ppm, and 40 ppm, but not in 80 ppm. For the resistant test against gentamycin, the resistent zone was 15 mm. Conclusion: Bacillus bacteria were resistent to mercury of 10 ppm, 20 ppm, and 40 ppm, but were sensitive to mercury of 80 ppm and gentamycin.Keywords: bacillus, mercury, gentamicin, resistantAbstrak: Merkuri adalah unsur dengan nomor atom (N) 80 serta massa molekul relatif (MR) 200,59. Individu yang secara kontinu terkena paparan merkuri yang tinggi a. l. oleh konsumsi ikan yang mengandung merkuri menunjukkan kerusakan kognitif. Salah satu usaha untuk detoksifikasi merkuri dapat dilakukan dengan menggunakan mikroorganisme resisten merkuri seperti bakteri resisten merkuri. Bila merkuri terpapar dalam tubuh manusia dengan kadar kecil dalam waktu yang sangat lama dapat membuat bakteri tersebut beradaptasi bahkan mampu mereduksi merkuri tersebut sehingga menjadi tidak berbahaya. Penelitian ini bertujuan untuk mengetahui apakah bakteri Bacillus resisten terhadap merkuri dan gentamisin. Penelitian uji resistensi merkuri pada Bacillus dilakukan menggunakan media LB cair yang telah diinkubasi selama 24 jam dan didapatkan pada pengenceran 10 ppm, 20 ppm, dan 40 ppm ada koloni bakter atau resisten tetapi pada pengenceran 80 ppm tidak ada pertumbuhan koloni bakteri. Pada uji resistensi terhadap gentamisin menggunakan media LB padat yang telah diinkubasi selama 24 jam didapatkan hasil zona hambat gentamisin 15 mm. Simpulan: Bacillus resisten terhadap merkuri sampai dengan pengeceran 40 ppm tetapi tidak resisten pada merkuri pengenceran 80 ppm dan gentamisin.Kata kunci: bacillus, merkuri, gentamisin, resisten

scholarly journals Pengaruh pH dan Suhu terhadap Aktivitas Pereduksi Merkuri Bakteri Resisten Merkuri Tinggi Bacillus cereus yang Diisolasi dari Urin Pasien dengan Amalgam Gigi

e-GIGI ◽  
2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Billy J. Kepel ◽  
Widdhi Bodhi ◽  
. Fatimawali
Keyword(s):  
Bacillus Cereus ◽  
Incubation Temperature ◽  
Mercury Resistance ◽  
Resistant Bacteria ◽  
Growth Media ◽  
Mercury Compounds ◽  
Reducing Activity ◽  
Mercury Detoxification ◽  
Incubation Temperatures ◽  
Mercury Resistant Bacteria

Abstract: Mercury is a very toxic compound to humans, therefore, a method to overcome its presence in the environment is required. Detoxification of mercury can be done by using mercury resistant bacteria. Mercury-resistant bacteria Bacillus cereus isolate FUA have been obtained from the urine of patients with dental mercury amalgam. This study was aimed to determine the mercury detoxification activity of Bacillus cereus isolate FUA at varying pH medium and incubation temperature. The study was carried out by growing Bacillus cereus isolate FUA on oblique media, then were planted in the growth media of LB broth containing mercury compounds of 10 ppm HgCl2 with varying pHs of 5, 7, and 9 and incubation temperatures of 15, 25 and 35oC. The amount of bacterial growth was analyzed by using spectrophotometer and mercury levels were analyzed by using CV-AAS method. The results showed that the growth and mercury reducing activity of Bacillus cereus isolate FUA were optimum at pH 7 and incubation temperature of 35oC. In conclusion, the growth of Bacillus cereus isolate FUA and its mercury reducing activity were optimum at pH 7 and temperature of 35oC. It is expected that the results of this study can be the basis for further research on the process of mercury detoxificationKeywords: Bacillus cereus, urine, mercury resistance, pH, temperature Abstrak: Merkuri adalah senyawa yang sangat beracun bagi manusia sehingga diperlukan metode untuk mengatasi keberadaannya di lingkungan. Detoksifikasi merkuri dapat dilakukan dengan menggunakan bakteri resisten merkuri. Bakteri yang resisten merkuri Bacillus cereus isolat FUA telah diperoleh dari urin pasien dengan amalgam gigi. Penelitian ini bertujuan untuk mengetahui aktivitas detoksifikasi merkuri Bacillus cereus isolat FUA pada berbagai variasi pH medium dan suhu inkubasi. Bakteri Bacillus cereus isolat FUA ditumbuhkan pada media miring, kemudian ditanam pada media pertumbuhan bakteri LB broth yang mengandung senyawa merkuri 10 ppm HgCl2 dengan berbagai pH 5, 7 dan 9 dan suhu inkubasi 15, 25 dan 35oC. Jumlah pertumbuhan bakteri dianalisis menggunakan spektrofotometer dan kadar merkuri dianalisis menggunakan metode CV-AAS. Hasil penelitian menunjukkan bahwa aktivitas pertumbuhan dan aktivitas pereduksi merkuri Bacillus cereus isolat FUA optimum pada lingkungan pertumbuhan dengan pH 7 dan suhu inkubasi 35oC. Simpulan penelitian ini ialah aktivitas pertumbuhan dan pereduksi merkuri Bacillus cereus isolat FUA yang optimum pada pH 7 dan suhu 35oC. Diharapkan hasil penelitian ini dapat menjadi dasar untuk penelitian lebih lanjut tentang proses detoksifikasi merkuri.Kata kunci: Bacillus cereus, urin, resistensi merkuri, pH, suhu

scholarly journals Isolasi bakteri resisten merkuri pada urin pasien dengan tumpatan amalgam di puskesmas paniki bawah

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Intan P.R. Sompie ◽  
Billy J. Kepel ◽  
Fona Budiarso
Keyword(s):  
Human Life ◽  
Dental Amalgam ◽  
The Body ◽  
Resistant Bacteria ◽  
Toxic Heavy Metals ◽  
Primary Health ◽  
Physiology And Biochemistry ◽  
Dental Amalgam Fillings ◽  
Mercury Resistant Bacteria ◽  
Amalgam Fillings

Abstract: Mercury is one of the most toxic heavy metals. Although it can be harmful for human health, the use of mercury in daily life virtually covers all aspects of human life, including its use as the material for dental amalgam fillings. The mercury in amalgam may enter the body, be absorbed by the digestive tract, and then be excreted through the urine. There are bacteria known to be resistant to mercury. This stduy was aimed to obtain and identify the mercury-resistant bacteria in the urine of patients with amalgam fillings. This was a descriptive-explorative study using urine samples of 2 patients who had used amalgam fillings for at least 6 months at Puskesmas (primary health care) Paniki Bawah. The samples underwent morphology, physiology, and biochemistry tests at the Microbiology Laboratory of Pharmacy FMIPA Sam Ratulangi University. The results showd that there were 3 genus of mercury resistant bacteria that survived in 10 ppm, 20 ppm, and 40 ppm concentrations of mercury, as follows: Bacillus, Klebsiella, and Staphylococcus. Conclusion: There are 3 genus of mercury resistant bacteria in the urine of patients with amalgam dental fillings.Keywords: mercury, amalgam, urine, bacteria, resistant Abstrak: Merkuri merupakan salah satu jenis logam berat berbahaya. Walaupun diketahui memiliki dampak buruk bagi kesehatan manusia, namun pemanfaatan merkuri dalam kehidupan sehari-hari hampir meliputi semua aspek kehidupan manusia, termasuk dalam penggunaannya sebagai bahan tumpatan gigi amalgam. Merkuri dalam amalgam akan masuk ke dalam tubuh, diabsorbsi melalui saluran pencernaan, dan dieskresikan melalui urin. Diketahui bahwa terdapat bakteri yang resisten terhadap merkuri. Penelitian ini bertujuan untuk mengetahui dan mengidentifikasi bakteri resisten merkuri yang terdapat pada urin pasien dengan tumpatan amalgam. Jenis penelitian ialah deskriptif eksploratif dengan mengambil sampel urin pada 2 pasien yang menggunakan tumpatan amalgam minimal 6 bulan di Puskesmas Paniki Bawah. Sampel diuji secara morfologi, fisiologi, dan biokimia di Laboratorium Mikrobiologi Farmasi FMIPA Universitas Sam Ratulangi. Hasil penelitian mendapatkan dari berbagai uji yang dilakukan ditemukan 3 genus bakteri resisten merkuri yang bertahan pada konsentrasi merkuri 10 ppm, 20 ppm, dan 40 ppm, yaitu Bacillus, Klebsiella, dan Staphylococcus. Simpulan: Terdapat 3 genus bakteri resisten merkuri pada urin pasien dengan tumpatan amalgam di Puskesmas Paniki Bawah. Kata kunci: merkuri, amalgam, urin, bakteri, resisten

scholarly journals Biodetoxification of Toxic Heavy Metals by Marine Metal Resistant Bacteria- A Novel Approach for Bioremediation of the Polluted Saline Environment

2017 ◽  
pp. 343-376 ◽  
Author(s):  
Ranjan Kumar Mohapatra ◽  
Pankaj Kumar Parhi ◽  
Jayanta Kumar Patra ◽  
Chitta Ranjan Panda ◽  
H. N. Thatoi
Keyword(s):  
Heavy Metals ◽  
Resistant Bacteria ◽  
Toxic Heavy Metals ◽  
Saline Environment ◽  
Novel Approach

scholarly journals Removal of Hg(ii) in aqueous solutions through physical and chemical adsorption principles

RSC Advances ◽  
2019 ◽  
Vol 9 (36) ◽  
pp. 20941-20953 ◽  
Author(s):  
Mengdan Xia ◽  
Zhixin Chen ◽  
Yao Li ◽  
Chuanhua Li ◽  
Nasir M. Ahmad ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Aqueous Solutions ◽  
Mercury Pollution ◽  
Chemical Adsorption ◽  
Toxic Heavy Metals ◽  
P Adsorption ◽  
Physical And Chemical

Adsorption has been the focus of research on the treatment of heavy metal mercury pollution since it is among the most toxic heavy metals in existence.

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI

2011 ◽  
Vol 15 (1) ◽  
pp. 282 ◽  
Author(s):  
Fatimawali Fatimawali ◽  
Fatmawaty Badaruddin ◽  
Irawan Yusuf
Keyword(s):  
Klebsiella Pneumoniae ◽  
Pure Culture ◽  
Culture Media ◽  
Inorganic Mercury ◽  
River Estuary ◽  
Nutrient Broth ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Mercury Resistant Bacteria

ISOLASI DAN IDENTIFIKASI BAKTERI RESISTEN MERKURI DARI MUARA SUNGAI SARIO YANG DAPAT DIGUNAKAN UNTUK DETOKSIFIKASI LIMBAH MERKURI Fatimawali1), Fatmawaty Badaruddin2), Irawan Yusuf 2) 1)Dosen Fakultas  Kedokteran Unsrat Manado dan Mahasiswa Program Doktor Fakultas Kedokteran Universitas Hasanuddin Makassar; 2)Dosen Fakultas Kedokteran Universitas Hasanuddin Makassar ABSTRAK Merkuri adalah logam berat yang bersifat sangat toksik, mempunyai afinitas terhadap gugus thiol protein.  Keberadaan merkuri  dilingkungan, karena secara alamiah maupun karena aktifitas antrofogenik. Di Kabupaten Bolaang Mongondow terdapat beberapa penambang emas rakyat yang menggunakan merkuri untuk mengekstrak emas dari batuan atau ore dan membuang limbah merkuri secara bebas ke lingkungan dan terbawa air hujan sampai kepada perairan.  Sedimen perairan yang terkontaminasi merkuri dapat ditumbuhi bakteri resisten merkuri.  Bakteri resisten merkuri anorhanik dapat digunakan untuk bioremediasi perairan, karena bakteri tersebut mampu mereduksi merkuri anorganik menjadi logam merkuri yang mudah menguap dan kurangtoksik.  Dan merupakan sumber pencemaran merkuri.  Bakteri resisten merkuri mempunyai operon mer yang biasanya terkandung dalam plasmid.  Penelitian ini bertujuan untuk mengisolasi bakteri resisten merkuri anorganik, dan menganalisis kemampuannya dalam mereduksi HgCl2 dalam media nutrient broth.  Bakteri resisten merkuri tinggi, dilakukan analisis Gen 16S rRNAnya untuk mengetahuis pesiesnya.  Hasil penelitian menunjukkan bahwa isolat A1.1.1 merupakan bakteri spesies Klebsiella pneumoniae, mampu mereduksi HgCl2 75% dalam waktu 1 jam, 92% dalam waktu 12 jam dan 99,4%  dalam waktu 24 jam Kata Kunci: bakteri, detoksifikasi,  HgCl2,  Klebsiella pneumoniae   ISOLATION  AND  IDENTIFICATION OF MERCURY-RESISTANT BACTERIUM FROM SARIO RIVER ESTUARY THAT CAN BE USED TO DETOXIFY INORGANIK MERCURY WASTES ABSTRACT Mercury (Hg) is well known for its high toxicity and strong affinity toward the thiol group of proteins. When Hg released into the environment in substantial quantities through natural events and anthropogenic activities . In District of Bolaang Mongondow, many miners who are a source of mercury pollution of aquatic environments to which they use to extract gold from the rock or ore. Waters sediment contaminated with mercury, can be overgrown with microorganism such as bacteria that are resistant to mercury.  Mercury-resistant bacteria can be used for bioremediation of waters due to the ability of these bacteria to reduce toxic inorganic mercury into mercury metal which is volatile and less toxic. Mercury-resistant bacteria has mer Operon which usually contained in the plasmid.  The aim of this study was the selection of bacterial strains resistant to inorganic mercury, as well as to show their capacity to reduce mercury  in pure culture media Nutrient Broth. Twelve isolate bacteria was selected for its capacity to reduce mercury HgCl2 in culture media nutrient broth. The one bacterial strains belong to the spesies Klebsiella pneumoniae. Inoculated in pure culture, these strain showed a mercury reduction of 75% in 1 hour, 92% in 12 hours, and  99,4% in 24 hours. Keywords : bacteria , detoxification, HgCl2 ,  Klebsiella pneumoniae

ASSESSMENT OF HEAVY METAL CONCENTRATION IN COCONUT WATER

1970 ◽  
pp. 07-10
Author(s):  
MdDidarul Islam, Ashiqur Rahaman, Aboni Afrose
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Heavy Metal Concentration ◽  
Coconut Water ◽  
Toxic Heavy Metals ◽  
High Concentration ◽  
Toxic Heavy Metal ◽  
Low Concentration ◽  
Wet Ashing ◽  
Aas Method

This study was based on determining concentration of essential and toxic heavy metal in coconut water available at a local Hazaribagh area in Dhaka, Bangladesh. All essential minerals, if present in the drinking water at high concentration or very low concentration, it has negative actions. In this study, fifteen samples and eight heavy metals were analyzed by Atomic Absorption Spectroscopy (AAS) method which was followed by wet ashing digestion method. The concentration obtained in mg/l were in the range of 0.3 to 1.5, 7.77 to 21.2, 0 to 0.71, 0 to 0.9, 0 to 0.2, 0.9 to 17.3, 0.1 to 0.9, 0 to 0.9 and 0 to 0.7 for Fe, Ni, Cu, Cd, Cr, Zn, Pb and Se respectively. From this data it was concluded that any toxic heavy metals like Cd, Cr, Pb and Ni exceed their toxicity level and some essential nutrients were in low concentration in those samples. 

Sign in / Sign up

Export Citation Format

Share Document