Importance of the Cyclic AMP Concentration for the Rate of Lipolysis in Human Adipose Tissue

1980 ◽  
Vol 59 (3) ◽  
pp. 199-201 ◽  
Author(s):  
P. Arner ◽  
J. Östman
Keyword(s):  
Adipose Tissue ◽  
Cyclic Amp ◽  
Subcutaneous Adipose Tissue ◽  
Human Adipose Tissue ◽  
Strong Positive Correlation ◽  
Glycerol Release ◽  
Two Parameters ◽  
Subcutaneous Adipose ◽  
The Relationship ◽  
Release Of Glycerol

1. The activation of lipolysis on incubation of human subcutaneous adipose tissue was examined in terms of the relationship between the release of glycerol and the concentration of tissue cyclic AMP. 2. A strong positive correlation was obtained between the maximum concentration of cyclic AMP and the rate of glycerol release in the presence of noradrenaline (r = 0.9), whereas, in the basal state, these two parameters were only weakly correlated (r = 0.45). 3. It appears that the noradrenaline-induced rate of lipolysis depends upon the maximal concentration of cyclic AMP that is present in human adipose tissue.

METABOLISM IN ADIPOSE TISSUE AND MUSCLE OF THE YOUNG PIG

1990 ◽  
Vol 70 (1) ◽  
pp. 199-206 ◽  
Author(s):  
O. ADEOLA ◽  
B. W. McBRIDE ◽  
R. O. BALL ◽  
L. G. YOUNG
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Adipose Tissue ◽  
Key Words ◽  
Adenosine Deaminase ◽  
Subcutaneous Adipose Tissue ◽  
Muscle Metabolism ◽  
Acetate Incorporation ◽  
Glycerol Release ◽  
Subcutaneous Adipose

Subcutaneous adipose tissue and intercostal and sartorius muscles from five barrows and five gilts at 20 kg liveweight were used to study lipogenesis, lipolysis, Na+, K+-ATPase-dependent respiration and protein synthesis. Lipogenesis rate measured by 14C-acetate incorporation into lipid was similar between barrows and gilts; and 100 μg insulin per mL enhanced (P < 0.1) subcutaneous adipose tissue lipogenesis by 74%. Lipolysis rate quantitated by glycerol release was similar between barrows and gilts (3546 and 4160 nmol g−1 2 h−1). Adenosine deaminase and norepinephrine together enhanced adipose tissue lipolytic response by 102%. Fractional and absolute rates of protein synthesis were similar between barrows and gilts (3.24 and 3.69% d−1; 6.01 and 6.06 mg g−1 d−1); and between intercostal and sartorius muscles. Barrows had lower Na+, K+-ATPase-dependent respiration than gilts and the maintenance of Na+ and K+ transmembrane ionic gradient in the muscle preparations accounted for 23–26% of total respiration. Key words: Pigs, adipose tissue, skeletal muscle, metabolism

scholarly journals RANTES release by human adipose tissue in vivo and evidence for depot-specific differences

2009 ◽  
Vol 296 (6) ◽  
pp. E1262-E1268 ◽  
Author(s):  
Rana Madani ◽  
Kalypso Karastergiou ◽  
Nicola C. Ogston ◽  
Nazar Miheisi ◽  
Rahul Bhome ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Epicardial Adipose Tissue ◽  
Ex Vivo ◽  
Human Adipose Tissue ◽  
Fat Pad ◽  
Obese Subjects ◽  
Subcutaneous Adipose ◽  
Circulating Levels

Obesity is associated with elevated inflammatory signals from various adipose tissue depots. This study aimed to evaluate release of regulated on activation, normal T cell expressed and secreted (RANTES) by human adipose tissue in vivo and ex vivo, in reference to monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) release. Arteriovenous differences of RANTES, MCP-1, and IL-6 were studied in vivo across the abdominal subcutaneous adipose tissue in healthy Caucasian subjects with a wide range of adiposity. Systemic levels and ex vivo RANTES release were studied in abdominal subcutaneous, gastric fat pad, and omental adipose tissue from morbidly obese bariatric surgery patients and in thoracic subcutaneous and epicardial adipose tissue from cardiac surgery patients without coronary artery disease. Arteriovenous studies confirmed in vivo RANTES and IL-6 release in adipose tissue of lean and obese subjects and release of MCP-1 in obesity. However, in vivo release of MCP-1 and RANTES, but not IL-6, was lower than circulating levels. Ex vivo release of RANTES was greater from the gastric fat pad compared with omental ( P = 0.01) and subcutaneous ( P = 0.001) tissue. Epicardial adipose tissue released less RANTES than thoracic subcutaneous adipose tissue in lean ( P = 0.04) but not obese subjects. Indexes of obesity correlated with epicardial RANTES but not with systemic RANTES or its release from other depots. In conclusion, RANTES is released by human subcutaneous adipose tissue in vivo and in varying amounts by other depots ex vivo. While it appears unlikely that the adipose organ contributes significantly to circulating levels, local implications of this chemokine deserve further investigation.

scholarly journals Oscillations of Fatty Acid and Glycerol Release From Human Subcutaneous Adipose Tissue In Vivo

Diabetes ◽  
2005 ◽  
Vol 54 (5) ◽  
pp. 1297-1303 ◽  
Author(s):  
F. Karpe ◽  
B. A. Fielding ◽  
S. W. Coppack ◽  
V. J. Lawrence ◽  
I. A. Macdonald ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Subcutaneous Adipose Tissue ◽  
Glycerol Release ◽  
Subcutaneous Adipose

Monocyte-associated chemokine expression in cells from subcutaneous adipose tissue isolated from operation material in patients during abdominoplasty

2017 ◽  
pp. 70-78
Author(s):  
А.Е. Копасов ◽  
С.Н. Блохин ◽  
С.Г. Морозов
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Aesthetic Surgery ◽  
Normal Body ◽  
Chemokine Expression ◽  
Normal Body Weight ◽  
Functional Features ◽  
Subcutaneous Adipose ◽  
The Relationship

Цель работы - изучение взаимосвязи между уровнем хемокинов и интенсивностью воспалительного процесса в подкожно-жировой ткани (ПЖТ) у лиц с ожирением и нормальной массой тела. Задача работы - определение уровня экспрессии хемокинов, сопряженных с моноцитами/макрофагами, и их рецепторов в клетках ПЖТ, выделенных при проведении абдоминопластики. Пациенты. В период с 2013 по 2017 гг. в Клинике пластической и эстетической хирургии проведены операции абдоминопластики у 262 женщин, биологический материал которых использован в работе. Методы. Состав тела и процент жировой массы определяли методом биоимпедансного анализа. Из образцов ПЖТ из операционного материала выделяли клетки, окрашивали их меченными флуоресцеинами антителами к хемокинам и анализировали на проточном цитометре FACSCalibur по программе SimulSet. Статистический анализ проводили по программе ANOVA. Результаты. Показано, что наличие ожирения оказывает влияние на клеточный состав ПЖТ. Экспрессия хемокинов семейства CC, а также рецепторов хемокинов CCR1, CCR2 и CCR5 в ПЖТ у пациентов с ожирением достоверно выше, чем у пациентов с нормальной массой тела. Заключение. Различия в экспрессии хемокинов на клетках ПЖТ у пациентов с ожирением или с нормальной массой тела отражают функциональные особенности ПЖТ и могут оказывать влияние на развитие осложнений после проведения операции абдоминопластики. AIM: we study the relationship between chemokines and intensity of the inflammatory process in the subcutaneous adipose tissue (SAT) in individuals with obesity and normal body weight. Objective: we determined the expression levels of chemokines associated with monocyte/macrophages, as well as their receptors on the cells of SAT, that were obtained during abdominoplasty. Patients. In this work we have used the biological material of 262 women to whom has been performed an abdominoplasty in the Clinic of plastic and aesthetic surgery from 2013 to 2017 years. Methods. Body composition and the weight of body fat were determined by bioimpedance analysis. SATs have been obtained during abdominoplasty surgery. SAT cells were isolated followed by the staining with fluoresceine labeled antibodies which fluorescence was analyzed using a flow cytometer FACSCalibur according to the program SimulSet. Statistical analysis was carried out using ANOVA. Results. It has been shown that the progressing obesity may affect the SAT cellular composition. The expression of chemokines from CC family and its receptors (CCR1, CCR2 and CCR5) on cells from SAT were significantly higher in patients with obesity compared to the normal body weight patients. Conclusions. Differences in the chemokine expression on SAT cells between patients with obesity and with normal body weight may reflect the functional features of SAT itself and can modify the complication developments after abdominoplasty.

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