Maggots and wound healing: an investigation of the effects of secretions fromLucilia sericatalarvae upon interactions between human dermal fibroblasts and extracellular matrix components

AbstractInspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration.

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The Role of the Extracellular Matrix Components in Cutaneous Wound Healing

BioMed Research International ◽

10.1155/2014/747584 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 113

Author(s):

Pawel Olczyk ◽

Łukasz Mencner ◽

Katarzyna Komosinska-Vassev

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Growth Factors ◽

Wound Repair ◽

Structural Integrity ◽

Healing Process ◽

Cellular Functions ◽

Extracellular Matrix Components ◽

Essential Components ◽

Matrix Components

Wound healing is the physiologic response to tissue trauma proceeding as a complex pathway of biochemical reactions and cellular events, secreted growth factors, and cytokines. Extracellular matrix constituents are essential components of the wound repair phenomenon. Firstly, they create a provisional matrix, providing a structural integrity of matrix during each stage of healing process. Secondly, matrix molecules regulate cellular functions, mediate the cell-cell and cell-matrix interactions, and serve as a reservoir and modulator of cytokines and growth factors’ action. Currently known mechanisms, by which extracellular matrix components modulate each stage of the process of soft tissue remodeling after injury, have been discussed.

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Role of Interactions Between Integrins and Extracellular Matrix Components in Healthy Epithelial Tissue and Establishment of a Long Junctional Epithelium During Periodontal Wound Healing: A Review

Journal of Periodontology ◽

10.1902/jop.1999.70.12.1511 ◽

1999 ◽

Vol 70 (12) ◽

pp. 1511-1522 ◽

Cited By ~ 28

Author(s):

Hans-Georg Gräber ◽

Georg Conrads ◽

Jens Wilharm ◽

Friedrich Lampert

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Epithelial Tissue ◽

Junctional Epithelium ◽

Extracellular Matrix Components ◽

Matrix Components ◽

Periodontal Wound Healing

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Enhanced extracellular matrix synthesis using collagen dressings loaded with Artemisia absinthium plant extract

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911518783216 ◽

2018 ◽

Vol 33 (5) ◽

pp. 516-528 ◽

Cited By ~ 3

Author(s):

Alexandra Gaspar-Pintiliescu ◽

Ana-Maria Seciu ◽

Florin Miculescu ◽

Lucia Moldovan ◽

Elena Ganea ◽

...

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Human Skin ◽

Composite Scaffold ◽

Dermal Fibroblasts ◽

Environment Interaction ◽

High Porosity ◽

Skin Cells ◽

Extracellular Matrix Components ◽

Human Skin Cells

The aim of this study was to develop three-dimensional porous composites of collagen (Col) incorporating polyphenolic-rich wormwood extract and to investigate their interaction with human skin cells, in order to optimize wound healing treatments. The scaffolds’ ultrastructure was observed by scanning electron microscopy, and biodegradability and bioactive compounds release were investigated in physiologic environment. Interaction of composites in direct and indirect contact with human skin cells was evaluated using two in vitro experimental models. ColWE scaffolds presented high porosity, swelling degree, and increased stability against enzymatic degradation, compared to Col scaffold. Composite scaffolds incorporating higher quantities of wormwood extract allowed better control of polyphenolics release. ColWE 0.5 variant favored the attachment and proliferation of human dermal fibroblasts and keratinocyte cells. In addition, the composite scaffold stimulated the synthesis of skin extracellular matrix components. All these results demonstrated that ColWE composites with improved physico-chemical and biological properties could be used in advanced wound healing applications.

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Polymeric Biomaterials for Wound Healing Incorporating Plant Extracts and Extracellular Matrix Components

10.5772/intechopen.98556 ◽

2021 ◽

Author(s):

Margaret O. Ilomuanya ◽

Ibilola M. Cardoso-Daodu ◽

Uloma N. Ubani-Ukoma ◽

Adannaya C. Adebona

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Plant Extracts ◽

Antimicrobial Agents ◽

Skin Diseases ◽

Healing Process ◽

Polymeric Biomaterials ◽

Extracellular Matrix Components ◽

Matrix Components ◽

Permanent Implant

Biomaterials are constructed to promote or stimulate the processes of wound healing. Polymeric biomaterials can be used to hydrate the wound and serve as barrier to pathogens with plant extracts, antimicrobial agents and extracellular components incorporated to stimulate the healing process. The biological and physical augmentation provided by extracellular matrix derived implants continues facilitate innovation in biomaterials utilized in management of nonhealing wounds. Tissue-processing methodologies can birth extracellular matrix-based devices with characteristic post-implantation responses ranging from the classic foreign body encapsulation of a permanent implant, to one where the implant is degraded and resorbed, to one where the processed extracellular matrix implant is populated by local fibroblasts and supporting vasculature to produce, a viable and metabolically active tissue. Extracellular matrix components and plant extracts have been shown to possesses pharmacological properties with potential for use in the treatment of skin diseases and wound healing. Antioxidant, anti-inflammatory assays, and wound healing assays have been shown to support the dermatological and wound healing usage of these medicinal plants extracts.

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