Influence of aldehyde fixation on the morphology of endosomes and lysosomes: quantitative analysis and electron tomography

In the present work a quantitative analysis of γ' and γ" phase nanoparticles in Inconel 718 was performed using two methods of electron tomography, namely EFTEM and FIB tomography. 3D visualisation showed that γ' particles are spherical, while γ" precipitates are disc-shaped. The values of mean size of γ' and γ" phase particles, determined by both electron tomography methods applied, are in good agreement.

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Quantitative analysis of cytoskeletal reorganization during epithelial tissue sealing by large-volume electron tomography

Nature Cell Biology ◽

10.1038/ncb3159 ◽

2015 ◽

Vol 17 (5) ◽

pp. 605-614 ◽

Cited By ~ 37

Author(s):

Mikhail Eltsov ◽

Nadia Dubé ◽

Zhou Yu ◽

Laurynas Pasakarnis ◽

Uta Haselmann-Weiss ◽

...

Keyword(s):

Quantitative Analysis ◽

Large Volume ◽

Electron Tomography ◽

Epithelial Tissue ◽

Cytoskeletal Reorganization ◽

Tissue Sealing

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Cellular Contacts Within the Interhemal Membrane of the Rat Placenta at Term

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050743 ◽

1974 ◽

Vol 32 ◽

pp. 146-147

Author(s):

William P. Jollie

Keyword(s):

Osmium Tetroxide ◽

Uranyl Acetate ◽

Capillary Endothelium ◽

En Bloc ◽

Aldehyde Fixation ◽

Chorioallantoic Placenta

By routine EM preparative techniques, the tissues which, collectively, separate maternal and fetal bloods in the fully formed chorioallantoic placenta of the rat have been shown to consist of three chorionic layers, or trophoblast, and a layer of allantoic capillary endothelium [Fig. 1]. Relationships between these layers are best demonstrated by special techniques, viz., cacodylate-buffered aldehyde fixation, collidine-buffered osmium tetroxide postfixation, and en bloc staining with uranyl acetate. By using this method on placentas at term, the cells of the outermost chorionic layer (Trophoblast 1) appear to be attached to each other by means of maculae adherentes which sometimes occur in clusters [Fig. 2].

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An Improved Quantitative Image Analyser

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100078663 ◽

1977 ◽

Vol 35 ◽

pp. 226-227

Author(s):

J.P. Fallon ◽

P.J. Gregory ◽

C.J. Taylor

Keyword(s):

Feature Extraction ◽

Quantitative Analysis ◽

Frequency Content ◽

General Sense ◽

Physical Measurements ◽

Quantitative Image ◽

Image Analyser ◽

Automatic Methods ◽

Quantitative Results

Quantitative image analysis systems have been used for several years in research and quality control applications in various fields including metallurgy and medicine. The technique has been applied as an extension of subjective microscopy to problems requiring quantitative results and which are amenable to automatic methods of interpretation.Feature extraction. In the most general sense, a feature can be defined as a portion of the image which differs in some consistent way from the background. A feature may be characterized by the density difference between itself and the background, by an edge gradient, or by the spatial frequency content (texture) within its boundaries. The task of feature extraction includes recognition of features and encoding of the associated information for quantitative analysis.Quantitative Analysis. Quantitative analysis is the determination of one or more physical measurements of each feature. These measurements may be straightforward ones such as area, length, or perimeter, or more complex stereological measurements such as convex perimeter or Feret's diameter.

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Quantification of Silica Uptake by Alveolar Macrophages - An Emperical Scanning Electron Microprobe Method

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100054297 ◽

1982 ◽

Vol 40 ◽

pp. 332-333

Author(s):

V. V. Damiano ◽

R. P. Daniele ◽

H. T. Tucker ◽

J. H. Dauber

Keyword(s):

Quantitative Analysis ◽

Alveolar Macrophages ◽

Bulk Sample ◽

Silica Particles ◽

Empirical Method ◽

Phagocytic Cells ◽

Calibration Standards ◽

X Ray ◽

Accurate Quantitative Analysis ◽

Scanning Electron

An important example of intracellular particles is encountered in silicosis where alveolar macrophages ingest inspired silica particles. The quantitation of the silica uptake by these cells may be a potentially useful method for monitoring silica exposure. Accurate quantitative analysis of ingested silica by phagocytic cells is difficult because the particles are frequently small, irregularly shaped and cannot be visualized within the cells. Semiquantitative methods which make use of particles of known size, shape and composition as calibration standards may be the most direct and simplest approach to undertake. The present paper describes an empirical method in which glass microspheres were used as a model to show how the ratio of the silicon Kα peak X-ray intensity from the microspheres to that of a bulk sample of the same composition correlated to the mass of the microsphere contained within the cell. Irregular shaped silica particles were also analyzed and a calibration curve was generated from these data.

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Lateral resolution of the electron microbeam analysis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100109859 ◽

1978 ◽

Vol 36 (1) ◽

pp. 542-543

Author(s):

H.J. Dudek

Keyword(s):

Quantitative Analysis ◽

Depth Resolution ◽

Lateral Resolution ◽

Cylindrical Particle ◽

Correction Procedure ◽

X Ray ◽

Element Concentrations ◽

Microbeam Analysis ◽

The Matrix

The chemical inhomogenities in modern materials such as fibers, phases and inclusions, often have diameters in the region of one micrometer. Using electron microbeam analysis for the determination of the element concentrations one has to know the smallest possible diameter of such regions for a given accuracy of the quantitative analysis.In th is paper the correction procedure for the quantitative electron microbeam analysis is extended to a spacial problem to determine the smallest possible measurements of a cylindrical particle P of high D (depth resolution) and diameter L (lateral resolution) embeded in a matrix M and which has to be analysed quantitative with the accuracy q. The mathematical accounts lead to the following form of the characteristic x-ray intens ity of the element i of a particle P embeded in the matrix M in relation to the intensity of a standard S

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