scholarly journals Cytological diagnosis of infectious conjunctivitis in cats

2019 ◽  
Vol 135 ◽  
pp. 01073
Author(s):  
Sergey Kartashov ◽  
Anastasia Rakitjanskaya ◽  
Marina Petrova ◽  
Maria Sultanova ◽  
Maria Oboeva
Keyword(s):  
Rapid Diagnosis ◽  
Cytological Diagnosis ◽  
Cotton Swab ◽  
Cytological Examination ◽  
Feasible Method ◽  
Non Invasive ◽  
Sterile Cotton Swab

Cytological examination of conjunctival smears obtained from cats with conjunctivitis can reveal the severity and type of inflammation and diagnose conjunctivitis caused by Chlamydophila felis. The diagnosis of chlamydia was made by the study of material taken from the conjunctival sac with a sterile cotton swab by PCR. Material for cytological examination was taken from these cats. Cytological examination of inclusions interpreted as chlamydial, was detected in all cytologic smears from sick cats, and has not been detected in none of healthy cats. Thus, cytological examination is a convenient, non-invasive, easily feasible method, which is suitable for rapid diagnosis of chlamydial conjunctivitis in cats.

CYTOLOGICAL DIAGNOSIS AS A METHOD OF SCREENING OF PRE-MALIGNANT MELANOCYTIC TUMORS AND EARLY FORMS OF SKIN MELANOMA

2018 ◽  
Vol 64 (3) ◽  
pp. 384-387
Author(s):  
Viktor Novik ◽  
D. Dreval
Keyword(s):  
Laser Therapy ◽  
Histological Examination ◽  
Clinical Data ◽  
Surgical Excision ◽  
Cytological Diagnosis ◽  
Skin Melanoma ◽  
Cytological Examination ◽  
Melanocytic Tumors ◽  
Quality Character ◽  
Atypical Nevi

Cytohistological comparisons to the account of the clinical data and revision of cytological and histological preparations on a material received from 21 patients are made. Cytomorphological features of juvenile nevi (Spitz-nevus, Reed-nevus), dysplastic and atypical nevi and early forms of melanoma are described. The establishment at cytological examination of good-quality character of melanocytic defeats at the account of the clinical data could be the basis for appointment laser therapy. At revealing of atypical melanocytes in cytological preparations patients should be referred to specialized oncological institutions for surgical excision of tumor with the subsequent histological examination. Thus cytological examination could be used in dermatological practice as a method of screening pre-malignant melanocytic tumors and skin melanoma.

scholarly journals Ganglion Cytology: A Novel Rapid Method for the Diagnosis of Equine Dysautonomia

2018 ◽  
Vol 56 (2) ◽  
pp. 244-247
Author(s):  
Chiara Piccinelli ◽  
Rachel Jago ◽  
Elspeth Milne
Keyword(s):  
Gold Standard ◽  
Neuronal Degeneration ◽  
Final Diagnosis ◽  
Rapid Diagnosis ◽  
Cytological Examination ◽  
Postmortem Diagnosis ◽  
Grass Sickness ◽  
Cervical Ganglion ◽  
Hematoxylin And Eosin ◽  
Cranial Cervical Ganglion

Equine dysautonomia (grass sickness) is characterized by autonomic neuronal degeneration and is often fatal. As outbreaks occur, rapid diagnosis is essential but confirmation currently requires histological examination. This study evaluated diagnostic accuracy of cytological examination of cranial cervical ganglion (CCG) scrapings for dysautonomia diagnosis. CCG smears from 20 controls and 16 dysautonomia cases were stained with May-Grünwald Giemsa (MGG), hematoxylin and eosin (HE), and cresyl fast violet (CFV), with HE-stained histological sections of CCG as gold standard for diagnosis. Examining all 3 stains together, the sensitivity and specificity were 100%. Occasional individual smears (4/107, 3.7%) were nondiagnostic due to low cellularity, and in a few individual smears the final diagnosis was correct but more tentative (CFV: 5/33 [15.1%], HE: 2/34 [5.9%], and MGG: 4/36 [11.1%]), due to low cellularity or suboptimal cell morphology. CCG cytology was considered reliable for rapid postmortem diagnosis of equine dysautonomia, particularly using MGG.

Expression of maspin in invasive fungal rhinosinusitis

2016 ◽  
Vol 131 (2) ◽  
pp. 150-154 ◽  
Author(s):  
Y D Huang ◽  
H W Yu ◽  
S W Xia ◽  
Z H Kang ◽  
Y S He ◽  
...  
Keyword(s):  
Immunohistochemical Staining ◽  
Critical Value ◽  
Rapid Diagnosis ◽  
Control Group ◽  
Non Invasive ◽  
Fungal Rhinosinusitis ◽  
Staining Score ◽  
Maspin Expression ◽  
Experimental Group ◽  
Invasive Fungal Rhinosinusitis

AbstractObjective:This study aimed to test the expression of maspin in invasive fungal rhinosinusitis and explore its value in diagnosing invasive fungal rhinosinusitis.Methods:Forty-two fungal rhinosinusitis cases (12 invasive and 30 non-invasive) were selected as the experimental group, and 30 chronic rhinosinusitis cases comprised the control group. Maspin expression was assessed in nasal mucous membrane specimens by immunohistochemical staining.Results:Compared with the control group, maspin expression was down-regulated in the fungal rhinosinusitis group (p < 0.05). Furthermore, the staining score for maspin was lowest in the invasive fungal rhinosinusitis group, as compared with both the non-invasive fungal rhinosinusitis group and the control group (p < 0.05). A maspin staining score of 5.70 was the critical value for diagnosis of invasive fungal rhinosinusitis, with sensitivity and specificity of 91.7 per cent and 88.3 per cent, respectively.Conclusion:The results of this study suggest that the maspin staining score may be a biomarker for effective and rapid diagnosis of invasive fungal rhinosinusitis.

scholarly journals Utility of contrast-enhanced echocardiography in the diagnosis and follow-up of a pseudoaneurysm of the aortic arch

2021 ◽  
Author(s):  
Yu Wu ◽  
Yuman Li ◽  
Jia Xu ◽  
Wang Menghe ◽  
Qing Lv
Keyword(s):  
Aortic Arch ◽  
Rapid Diagnosis ◽  
Non Invasive ◽  
Contrast Enhanced ◽  
Contrast Enhanced Echocardiography ◽  
Life Threatening

Spontaneous pseudoaneurysm of the aortic arch is an exceptionally rare and potentially life-threatening condition.Diagnosis of pseudoaneurysms of the aortic arch mainly depends on imaging examinations.Contrast-enhanced echocardiography, as a non-invasive, bed-side and radiation-free tool, plays an important role in the rapid diagnosis and postoperative follow-up of pseudoaneurysms of the aortic arch.

scholarly journals Clinical-Pathological and Molecular Evaluation of 451 NIFTP Patients from a Single Referral Center

Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 420
Author(s):  
Paola Vignali ◽  
Agnese Proietti ◽  
Elisabetta Macerola ◽  
Anello Marcello Poma ◽  
Liborio Torregrossa ◽  
...  
Keyword(s):  
Retrospective Cohort ◽  
Thyroid Neoplasms ◽  
Molecular Profile ◽  
Follicular Neoplasm ◽  
Cytological Diagnosis ◽  
Careful Monitoring ◽  
Non Invasive ◽  
Indeterminate Cytology ◽  
Nuclear Features ◽  
Molecular Evaluation

Background: Non-invasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTPs) were introduced in thyroid pathology in 2016. NIFTPs are a group of follicular neoplasm with an indolent behaviour. In this study, we gathered a large retrospective cohort of NIFTPs and compared those presenting as solitary lesions and NIFTPs found in multifocal setting. Methods: A retrospective search of NIFTPs was performed, and the clinico-pathological features were recorded. For a subgroup of patients, pre-surgical ultrasound (US) evaluation, cytological diagnosis, and molecular analysis were available. Results: We collected 451 NIFTPs; 254 (56.3%) were truly solitary tumours, while 197 coexisted with one or more NIFTP/cancer. Contrasting unifocal and multifocal settings, NIFTPs size was the only significantly different parameter. Preoperatively, NIFTP nodules mostly showed low-risk US characteristics, indeterminate cytology and a RAS-like molecular profile. Conclusion: NIFTPs often coexist with collateral thyroid tumours. However, no clinical-pathological differences can be observed between solitary and “multifocal” NIFTPs. Despite the well-established clinical indolence of NIFTP, a careful monitoring of the contralateral lobe should not be excluded.

Cytological diagnosis of microfilaria at unusual sites in clinically unsuspected cases: some rare presentations of filariasis

2018 ◽  
Vol 48 (4) ◽  
pp. 373-375 ◽  
Author(s):  
Shreekant Bharti ◽  
Neeraj Kumar Agarwal ◽  
Richa Katiyar ◽  
Saloni Dwivedi ◽  
Mridu Shri
Keyword(s):  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Needle Aspiration ◽  
Body Fluids ◽  
Cytological Diagnosis ◽  
Cytological Examination ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Needle Aspiration Cytology ◽  
Rule Out

Filariasis is a major concern, particularly in endemic regions. The majority of patients harbouring the parasite may remain asymptomatic for years. Here, we present six patients who were sent for routine cytological examination to rule out neoplastic pathology, in whom cytology identified filarial infestation. The main purposes of our paper are to highlight the importance of fine needle aspiration cytology (FNAC), a cheap and quick investigation to detect the microfilaria from tissue swelling and body fluids, and to point out that clinicians practising in endemic regions should always consider filarial infestation in patients presenting with longstanding swelling.

scholarly journals Accuracy of Two Point-of-Care Tests for Rapid Diagnosis of Bovine Tuberculosis at Animal Level using Non-Invasive Specimens

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Holden V. Kelley ◽  
Sarah M. Waibel ◽  
Sabeen Sidiki ◽  
Cristina Tomatis-Souverbielle ◽  
Julia M. Scordo ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Point Of Care ◽  
Rapid Diagnosis ◽  
Non Invasive ◽  
Point Of Care Tests

Assessment of conjunctival flora in eyes with lacrimal passage obstruction before and after successful dacryoendoscopic recanalisation

2020 ◽  
pp. bjophthalmol-2020-316264
Author(s):  
Takahiro Hiraoka ◽  
Sujin Hoshi ◽  
Kuniharu Tasaki ◽  
Tetsuro Oshika
Keyword(s):  
Bacterial Flora ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Cotton Swab ◽  
Drug Resistant Bacteria ◽  
Before And After ◽  
Conjunctival Flora ◽  
Culture Positivity Rate ◽  
Bacteriological Laboratory ◽  
Sterile Cotton Swab

BackgroundTo investigate conjunctival bacterial flora in eyes with lacrimal passage obstruction before and after dacryoendoscopic recanalisation with lacrimal tube intubation.MethodsOne-hundred fifty eyes with lacrimal passage obstruction that were successfully treated by dacryoendoscopic recanalisation were enrolled. Conjunctival sampling was done for each eye before and 4 months after surgery. The lower fornix was rubbed by a sterile cotton swab, and the collected samples were cultured with several agar plates. Colonies were differentiated and enumerated by standard bacteriological laboratory techniques.ResultsPositive bacterial growth was detected in 42.0% of all the samples before surgery, and the positivity rate significantly decreased to 26.0% after surgery (p=0.0051). The number of strains detected also decreased from 20 before surgery to 9 after surgery, especially pathogenic microorganisms decreased. In addition, drug-resistant bacteria such as penicillin-resistant Streptococcus pneumoniae and methicillin-resistant Staphylococcus aureus were detected in nine eyes before surgery but were detected only in one eye after surgery. Coagulase-negative Staphylococci and Corynebacterium spp., which are common in normal conjunctival flora, accounted for 46.5% of all the isolates before surgery and 80.9% after surgery, showing a significant increase in the rate after surgery (p<0.0001).ConclusionsThis study showed that physiological recanalisation of lacrimal passage after dacryoendoscopic surgery significantly decreased the culture positivity rate of conjunctival sac and the number of microorganism strains detected. It also decreased the number of potentially pathogenic and drug-resistant bacteria and increased the percentages of indigenous bacteria, causing the normalisation of conjunctival flora.

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