scholarly journals The influence of biofungicide and chemical fungicides on the manifestation of diseases and the yield of soybeans

2021 ◽  
Vol 247 ◽  
pp. 01046
Author(s):  
Svetlana Rezvyakova ◽  
Leonid Eremin ◽  
Petr Matveychuk ◽  
Elena Mitina

This study aims to improve the technology for protecting soybeans from fungal diseases based on new fungicides. The relevance of research is specified by a significant increase in the area occupied by soybeans and the need to protect crops from diseases. The experimental site is located in the territory of Oryol State Agrarian University (Lavrovo village of Oryol Region, Russia). The crop rotation is grain-fallow, the predecessor is winter wheat. The soil type is dark gray forest medium loamy. Soil acidity is 5.7. It has the following content of macronutrients: 11.5 mg/100 g of soil for P2O5, 10.9 mg/100 g of soil for K2O, 4.1% for humus. The Mezenka soybean was used, seeds of the 1st reproduction. Planting was carried out in the first decades of May 2019 and 2020. We used the Maksim, KS protectant (Fludioxonil, 25 g/l). Fungicides Propuls, SE (Prothioconazole, 125 g/l, Fluopyram, 125 g/l), Vintazh, ME (Difenoconazole, 65 g/l, Flutriafol, 25 g/l) and biofungicide Vitaplan, SP (Bacillus subtilis strain VKM-V-2604D, titer 1010 CFU/g and Bacillus subtilis strain VKM-B-2605D, titer 1010 CFU/g) were used to treat plants at the end of June in the branching phase and again two weeks later in July in the budding phase. The treatment of soybean seeds with the fungicide Maksim, KS helped to protect the crop from fungal diseases until the first ten days of July. Two-fold treatment of crops with biofungicide Vitaplan and chemical fungicides Propuls and Vintazh had a significant impact on the prevalence and development of fungal diseases. The biological effectiveness of chemical fungicides in comparison with biological was higher in the phase of complete formation of beans - the beginning of ripening by 9 and 15%, respectively. In comparison with the control, the increase in the yield of soybeans was 10.4% using Vitaplan, 16.4% using Propuls, 17.9% using Vintazh.

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Haruka Sugiura ◽  
Ayumi Nagase ◽  
Sayoko Oiki ◽  
Bunzo Mikami ◽  
Daisuke Watanabe ◽  
...  

Abstract Saprophytic bacteria and plants compete for limited nutrient sources. Bacillus subtilis grows well on steamed soybeans Glycine max to produce the fermented food, natto. Here we focus on bacterial responses in conflict between B. subtilis and G. max. B. subtilis cells maintained high growth rates specifically on non-germinating, dead soybean seeds. On the other hand, viable soybean seeds with germinating capability attenuated the initial growth of B. subtilis. Thus, B. subtilis cells may trigger saprophytic growth in response to the physiological status of G. max. Scanning electron microscope observation indicated that B. subtilis cells on steamed soybeans undergo morphological changes to form apertures, demonstrating cell remodeling during saprophytic growth. Further, transcriptomic analysis of B. subtilis revealed upregulation of the gene cluster, yesOPQR, in colonies growing on steamed soybeans. Recombinant YesO protein, a putative, solute-binding protein for the ATP-binding cassette transporter system, exhibited an affinity for pectin-derived oligosaccharide from plant cell wall. The crystal structure of YesO, in complex with the pectin oligosaccharide, was determined at 1.58 Å resolution. This study expands our knowledge of defensive and offensive strategies in interspecies competition, which may be promising targets for crop protection and fermented food production.


2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Daniel R. Reuß ◽  
Andrea Thürmer ◽  
Rolf Daniel ◽  
Wim J. Quax ◽  
Jörg Stülke

Bacillus subtilis ∆6 is a genome-reduced strain that was cured from six prophages and AT-rich islands. This strain is of great interest for biotechnological applications. Here, we announce the full-genome sequence of this strain. Interestingly, the conjugative element ICE Bs 1 has most likely undergone self-excision in B. subtilis ∆6.


1979 ◽  
Vol 179 (2) ◽  
pp. 333-339 ◽  
Author(s):  
A Y Strongin ◽  
D I Gorodetsky ◽  
I A Kuznetsova ◽  
V V Yanonis ◽  
Z T Abramov ◽  
...  

Intracellular serine proteinase was isolated from sporulating cells of Bacillus subtilis Marburg 168 by gramicidin S-Sepharose 4B affinity chromatography. The enzymological characteristics, the amino acid composition and the 19 residues of the N-terminal sequence of the enzyme are reported. The isolated proteinase was closely related to, but not completely identical with, the intracellular serine proteinase of B. subtilis A-50. The divergence between these two intracellular enzymes was less than that between the corresponding extracellular serine proteinases (subtilisins) of types Carlsberg and BPN′!, produced by these bacterial strains. This may be connected with the more strict selection constraints imposed in intracellular enzymes during evolution.


2017 ◽  
Vol 12 (1) ◽  
pp. 255-263 ◽  
Author(s):  
Kanjana Thumanu ◽  
Darawadee Wongchalee ◽  
Mathukorn Sompong ◽  
Piyaporn Phansak ◽  
Toan Le Thanh ◽  
...  

Microbiology ◽  
2008 ◽  
Vol 154 (1) ◽  
pp. 54-63 ◽  
Author(s):  
Kazuo Kobayashi ◽  
Ritsuko Kuwana ◽  
Hiromu Takamatsu

Author(s):  
Meichun Chen ◽  
Meixia Zheng ◽  
Yanping Chen ◽  
Rongfeng Xiao ◽  
Xuefang Zheng ◽  
...  

2018 ◽  
Vol 7 (18) ◽  
Author(s):  
Haeyoung Jeong ◽  
Da-Eun Jeong ◽  
Seung-Hwan Park ◽  
Seong Joo Kim ◽  
Soo-Keun Choi

Bacillus subtilis WB800N is a genetically engineered variant of B. subtilis 168, such that all extracellular proteases are disrupted, which enables WB800N to be widely used for the expression of secretory proteins. Here, we report the 4.2-Mb complete genome sequence of WB800N and present all of the disrupted gene structure.


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