scholarly journals First report on the sero-epidemiology of Toxoplasma gondii infection in German roe deer (Capreolus capreolus)

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 52 ◽  
Author(s):  
Mike Heddergott ◽  
Peter Steinbach ◽  
Daniel Pohl ◽  
Alain C. Frantz
Keyword(s):  
Toxoplasma Gondii ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Human Infection ◽  
German Population ◽  
Wild Ungulates ◽  
Age Classes ◽  
Game Species ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

While the roe deer (Capreolus capeolus) is the most important game species in Germany and its venison is popular, there is limited knowledge about the prevalence of Toxoplasma gondii in this animal population in the country, and in wild ungulates in Germany generally. Between 2013 and 2015, we collected 295 blood samples from roe deer belonging to a central German population. Sera were analysed using a modified agglutination test (MAT, cut-off 1:20), and antibodies were detected in 86 of the 295 samples (29%). Seroprevalence values differed significantly between the different age classes, with antibodies more frequently observed in adults. In contrast, seroprevalence did not differ significantly between the sexes or collection years. Venison is frequently consumed raw or undercooked and may be a potential source of human infection with T. gondii.

scholarly journals Seroprevalence of Toxoplasma gondii in free-living European mouflon (Ovis orientalis musimon) hunted in central Germany

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 21 ◽  
Author(s):  
Mike Heddergott ◽  
Natalia Osten-Sacken ◽  
Peter Steinbach ◽  
Alain C. Frantz
Keyword(s):  
Toxoplasma Gondii ◽  
Human Infection ◽  
Wild Ungulates ◽  
Sample Collection ◽  
Age Classes ◽  
Free Living ◽  
Modified Agglutination Test ◽  
Potential Source ◽  
Significant Difference ◽  
Undercooked Meat

Despite increasing consumption of mouflon (Ovis orientalis musimon) meat in Germany, there is currently no surveillance of Toxoplasma gondii infection in populations of these animals and generally little knowledge about the prevalence of this protozoan in German wild ungulates. Between 2011 and 2015, we collected 138 blood samples from a free-living mouflon population in central German and tested sera for the presence of T. gondii antibodies using a modified agglutination test (MAT, cut-off 1:20). Antibodies were detected in 31 of the 138 samples (22.46%). There was a significant difference in seroprevalence between the different age classes, with antibodies to T. gondii more frequent in adults. In contrast, there was no significant difference in seroprevalence depending on sex and year of sample collection. Game meat is frequently consumed as raw or undercooked meat and may therefore represent a potential source of human infection with T. gondii.

scholarly journals Prevalence and risk factors for Toxoplasma gondii infection in certified and non-certified pig breeding farms in the Toledo microregion, PR, Brazil

2010 ◽  
Vol 19 (3) ◽  
pp. 152-156 ◽  
Author(s):  
Piassa Franciele Rossandra ◽  
Jacqueline Baptista de Araújo ◽  
Ronaldo César da Rosa ◽  
Rodrigo José Mattei ◽  
Rodrigo Costa da Silva ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Human Infection ◽  
Control Measures ◽  
Rodent Control ◽  
Reproductive Disorders ◽  
Toxoplasma Infection ◽  
Pork Products ◽  
Toxoplasma Gondii Infection ◽  
Breeding Farms

Toxoplasma gondii infection has been diagnosed in pigs all over the world. Economical losses are generally related to reproductive disorders. Toxoplasma infection is also a matter of public health because tissue cysts of the parasite may remain in pork and pork products, and become sources of human infection. The objective of this study was to evaluate the frequency and risk factors associated with Toxoplasma infection in certified and non-certified pig breeding farms in the Toledo microregion, in the State of Paraná, Brazil which includes the cities of Toledo, Nova Santa Rosa, Sao José das Palmeiras and Sao Pedro do Iguaçu. Relative frequency of infection was 13.4%, independently of the type of farm. Logistic regression analysis showed that the following factors were associated with infection: absence of workers exclusive for each area of the farm, access of other animals to feeders and drinkers, lack of lids in drinkers, lack of rodent control measures, mean piglet number and weight at weaning per female.

scholarly journals Detection of Viable Toxoplasma gondii in Free-Range Pigs from the Special Nature Reserve of Zasavica

2016 ◽  
Vol 65 (3-4) ◽  
pp. 1-6 ◽  
Author(s):  
Ljiljana Kuruca ◽  
Ivana Klun ◽  
Aleksandra Uzelac ◽  
Aleksandra Nikolić ◽  
Branko Bobić ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Nature Reserve ◽  
Heart Tissue ◽  
Human Infection ◽  
Serological Screening ◽  
Free Range ◽  
Modified Agglutination Test ◽  
Successful Isolation ◽  
Polymerase Chain ◽  
Great Appreciation

SummaryToxoplasma gondii is considered one of the most successful parasites of humans and animals. The ingestion of viable cysts through the consumption of undercooked pork is recognized as a significant route of human infection with T. gondii. The aim of this study was to investigate the presence of viable parasite in tissues of free-range pigs from the Zasavica Special Nature Reserve. All pigs were of the Mangulica breed, raised in a traditional way. The serological screening was performed using a modified agglutination test (MAT). The isolation of viable T. gondii was attempted by a bioassay of pig heart tissue in mice, while the real-time polymerase chain reaction (qPCR) targeted at the 529 bp repetitive element of T. gondii was used to detect parasitic DNA in digested hearts. Specific antibodies were detected in 12 out of 18 pigs examined. The bioassay was performed for five MAT-positive and one MAT-negative pig, and a total of three isolates were obtained. qPCR was performed for all samples, including one MAT-negative sample that was not bioassayed. The presence of T. gondii DNA was confirmed in all hearts with a positive bioassay as well as in one originating from seropositive and one from seronegative pig whose hearts were not bioassayed. The successful isolation of viable cysts, presence of risk factors (such as older age at the time of slaughter) and increased contact with the environment, along with the great appreciation of Serbian consumers towards home-cured Mangulica’s meat, make this breed worthy of consideration as a potentially important reservoir of human infection.

Cross-sectional survey of Toxoplasma gondii infection in colony cats from urban Florence (Italy)

2010 ◽  
Vol 12 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Francesca Mancianti ◽  
Simona Nardoni ◽  
Gaetano Ariti ◽  
Dario Parlanti ◽  
Giovanna Giuliani ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Serum Samples ◽  
Cross Sectional Survey ◽  
Nested Polymerase Chain Reaction ◽  
Cross Sectional ◽  
Modified Agglutination Test ◽  
Faecal Samples ◽  
Key Species ◽  
Polymerase Chain ◽  
Toxoplasma Gondii Infection

Cats are the key species in the epidemiology of Toxoplasma gondii infection, even if the proportion of subjects excreting oocysts is low. The aim of the present paper was to obtain information about seroprevalence, oocyst shedding rate and presence of T gondii DNA in faeces collected from an urban population of colony cats in Florence (Tuscany). Fifty European shorthair feral cats were examined for anti- T gondii specific antibodies by a modified agglutination test (MAT), and for oocysts by microscopic examination and for faecal protozoal DNA, by means of a nested polymerase chain reaction (n-PCR) protocol. Twenty-two out of 50 serum samples (44%) were MAT positive. T gondii oocysts were not detected in any of the examined faecal samples. Eight out of 50 faecal specimens (16%) were n-PCR positive and sequencing of the bands was specific for T gondii. Detection by combination of the two methods was higher than single techniques and enhanced the detection of T gondii up to 48%. Our results suggest that the use of MAT plus PCR in faeces may be the best choice for diagnosis of feline toxoplasmosis. Further studies to ascertain the real infectivity of the copro-PCR positive subjects are required.

Seroprevalence and correlates of Toxoplasma gondii infection in dairy cattle in northwest China

2015 ◽  
Vol 60 (4) ◽  
Author(s):  
Qi-Dong Tan ◽  
Xiao-Yu Yang ◽  
Ming-Yang Yin ◽  
Ling-Ying Hu ◽  
Si-Yuan Qin ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Dairy Cattle ◽  
Epidemiological Data ◽  
Northwest China ◽  
Gansu Province ◽  
Serum Samples ◽  
Modified Agglutination Test ◽  
Significant Difference ◽  
Toxoplasma Gondii Infection

AbstractPrevalence of antibodies to Toxoplasma gondii and risk factors with infection were assessed in dairy cattle from Gansu Province and Ningxia Hui Autonomous Region (NXHAR), northwest China. In total, 1657 serum samples were collected and assayed by the modified agglutination test. The overall seroprevalence was 4.83% at a 1:100 cut-off, with titers of 1:100 in 72, 1:200 in 4, 1:400 in 4. Among the risk factors examined, no statistically significant difference was observed between T. gondii seroprevalence and regions or age of dairy cattle in the logistic regression analysis (P>0.05) and left out of the final model. However, numbers of pregnancies of dairy cattle was considered as main risk factor associated with T. gondii infection. Dairy cattle in nulliparity group (8.89%) had 6 times (OR=6.31, 95% CI, 2.69-14.83, P<0.001) higher risk of being seropositive compared to dairy cattle in 3 or above 3 pregnancies group (1.52%), followed by 1 pregnancy group (4.27%) had nearly 3 times (OR=2.89, 95% CI, 1.11-7.52, P = 0.03) higher risk of being seropositive compared to dairy cattle in 3 or above 3 pregnancies group, although no statistical difference was found between 2 pregnancies group and 3 or above 3 pregnancies group (P = 0.70). The results of this survey indicated the presence of T. gondii infection in dairy cattle in Gansu Province and NXHAR, which enriches the epidemiological data of T. gondii infection in dairy cattle in China, and is helpful to strengthen prevention and control of T. gondii infection in dairy cattle in these two regions.

scholarly journals SURVEI SEROLOGIS TOXOPLASMOSIS PADA TERNAK SAPI DI KABUPATEN PRINGSEWU MENGGUNAKAN METODE TOXOPLASMA MODIFIED AGGLUTINATION TEST (To-MAT)

2019 ◽  
Vol 7 (3) ◽  
pp. 270
Author(s):  
Galang B. ◽  
Emantis R. ◽  
Gina Dania P. ◽  
Endah S.
Keyword(s):  
Toxoplasma Gondii ◽  
Agglutination Test ◽  
Test Method ◽  
Cattle Population ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

The cattle population in Lampung Province has a relatively high number when compared to several other provinces. Pringsewu is one of the districts in Lampung Province having the supplier potential of beef in Lampung. The higher the population and production, could cause a potential infection of toxoplasmosis to livestock and human. So far the prevalence of toxoplasmosis in Pringsewu District. This study was conducted to determine the level of toxoplasmosis infection in cattle in Pringsewu district. This study used the To-MAT (Toxoplasma Modified Agglutination Test) method to test Toxoplasma gondii infection in blood of cows samples. The result of the study found that at a chronic level there were 23/24 cows positively infected with T.gondii. Whereas in acute level there are 8/24 cows positively infected with T. gondii. In general, the prevalence of toxoplasmosis is 95.8%. Keywords : Toxoplasmosis, Cow, To-MAT (Toxoplasma Modified Agglutination Test) method, Infection

scholarly journals Wild ungulates in Jordan: past, present, and forthcoming opportunities

2021 ◽  
Vol 13 (9) ◽  
pp. 19338-19351
Author(s):  
Ehab Eid ◽  
David Mallon
Keyword(s):  
Cervus Elaphus ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Fallow Deer ◽  
Current Status ◽  
Wild Ungulates ◽  
Equus Hemionus ◽  
Wild Ass ◽  
In The Wild ◽  
Arabian Oryx

Twelve species of ungulates are reported from the wild in Jordan. Three of these, Addax nasomaculatus (Addax), Bos primigenius (Aurochs), and Cervus elaphus (Red Deer) are known only from archaeological excavations. Dama mesopotamica (Mesopotamian Fallow Deer), Oryx leucoryx (Arabian Oryx) and Equus hemionus hemippus (Syrian Wild Ass) have been regionally extirpated in the wild. A semi-captive population of Persian Onager (E. h. hemionus) is held in Shumari Wildlife Reserve. The Arabian Oryx is also managed in semi-captive conditions in two reserves. Except the commonly occurring Wild Boar (Sus scrofa), other surviving ungulate species continue to be under serious threat. Gazella gazella (Palestinian Mountain Gazelle), Capreolus capreolus (European Roe Deer), Gazella marica (Arabian Sand Gazelle), and Gazella dorcas (Dorcas Gazelle) are Critically Endangered, and Capra nubiana (Nubian Ibex) is Endangered in the region. This paper provides a review of the historical and current status of wild ungulates in Jordan, listing the threats and conservation measures and provides recommendations for management and conservation in the future.

scholarly journals First report on seroprevalence and risk factors of Toxoplasma gondii infection in sheep and goats in North Lebanon

2019 ◽  
Vol 13 (09) ◽  
pp. 831-836 ◽  
Author(s):  
Dima El Safadi ◽  
Dany Abi Chahine ◽  
Alissar Al Tarraf ◽  
Omar Raii ◽  
Karim Mesto ◽  
...  
Keyword(s):  
Risk Factors ◽  
Risk Factor ◽  
Toxoplasma Gondii ◽  
Immunoglobulin G ◽  
Agglutination Test ◽  
Economic Losses ◽  
Transplacental Transmission ◽  
Igg Antibodies ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

Introduction: Toxoplasmosis is of dual importance in both public and veterinary health due to the respective risk of transplacental transmission in primo-infected pregnant women and economic losses caused by abortions in mammals. One of the main routes of Toxoplasma gondii transmission to humans is the consumption of raw or undercooked meats containing parasitic cysts. Here, we performed the first epidemiological study to determine the seroprevalence and the risk factors of toxoplasmosis in livestock in Lebanon. Methodology: Using a modified agglutination test with a cut-off of 1:40, we tested the positivity rate of Immunoglobulin G antibodies in the sera of 100 sheep and 80 goats collected from 18 different livestock farms located in North Lebanon between March and June 2018. Results: Anti-Toxoplasma gondii IgG antibodies were detected in 42% of sheep and 34% of goats. Adults (> 1 year) were significantly more infected by T. gondii than the lambs (< 1 year) in both species (p < 0.05). Conclusions: These findings indicated that food animals are highly exposed to T. gondii in Lebanon and could be potentially a major risk factor of T. gondii infection to humans. Consequently, national prophylactic strategies should be implemented to control and to prevent T. gondii transmission between animals and humans.

scholarly journals The effect of livestock on the physiological condition of roe deer (Capreolus capreolus) is modulated by habitat quality

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Fernando Horcajada-Sánchez ◽  
Gema Escribano-Ávila ◽  
Carlos Lara-Romero ◽  
Emilio Virgós ◽  
Isabel Barja
Keyword(s):  
Habitat Quality ◽  
Stress Responses ◽  
Roe Deer ◽  
Physiological Stress ◽  
Habitat Type ◽  
Capreolus Capreolus ◽  
Parasite Load ◽  
Livestock Grazing ◽  
Wild Ungulates ◽  
Fecal Cortisol

Abstract Free-range livestock grazing is a widespread human activity that not only modifies natural vegetation but also leads to interactions with wild ungulates. Most commonly, the interactions between cattle and wild ungulates have been studied with a focus on competition for high-quality forage. However, other mechanisms, such as the risk of parasite infection, might better describe this interaction. We aim to determine whether livestock affect roe deer (Capreolus capreolus Linnaeus, 1758) by reducing habitat quality and increasing the probability of infection by shared parasites. We measured noninvasive fecal cortisol metabolites as an indicator of habitat quality as well as the lung nematode larvae burden from the Dictyocaulus genus. A higher Dictyocaulus larvae load was found in the presence of livestock in pines, and feces collected in winter had a higher parasite load than feces collected in autumn. Additionally, fecal cortisol metabolite levels in the roe deer were affected by the interaction between habitat quality and livestock presence and were higher in the poorest habitat and when living in sympatry with cattle. Our results suggest that physiological stress responses in roe deer were mediated by the habitat type and the presence of competitors. The long-term implications of altered physiological responses such as those demonstrated here should be considered in management strategies for deer.

scholarly journals The First Report ofMycobacterium celatumIsolation from Domestic Pig (Sus scrofa domestica) and Roe Deer (Capreolus capreolus) and an Overview of Human Infections in Slovenia

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Mateja Pate ◽  
Manca Žolnir-Dovč ◽  
Darja Kušar ◽  
Brane Krt ◽  
Silvio Špičić ◽  
...  
Keyword(s):  
Sus Scrofa ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Invasive Disease ◽  
Human Infection ◽  
Domestic Pig ◽  
History Of ◽  
Serious Disease ◽  
Human Infections ◽  
Sus Scrofa Domestica

Mycobacterium celatum, a slowly growing potentially pathogenic mycobacterium first described in humans, is regarded as an uncommon cause of human infection, though capable of inducing invasive disease in immunocompromised hosts. According to some reports, a serious disease due toM. celatummay also occur in individuals with no apparent immunodeficiency. In animals, anM. celatum-related disease has been described in three cases only: twice in a domestic ferret (Mustela putorius furo) and once in a white-tailed trogon (Trogon viridis). In this paper, we report the first detection ofM. celatumin a domestic pig (Sus scrofa domestica) and roe deer (Capreolus capreolus). A nation-wide overview of humanM. celatuminfections recorded in Slovenia between 2000 and 2010 is also given. Pulmonary disease due toM. celatumwas recognized in one patient with a history of a preexisting lung disease.

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