Antimicrobial activity of Romanian propolis ethanolic extracts against E. coli isolated from bovine mastitis

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
M Niculae ◽  
CD Sandru ◽  
E Pall ◽  
S Popescu ◽  
L Stan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bovine Mastitis ◽  
E Coli ◽  
Ethanolic Extracts

scholarly journals In vitro Synergistic Antimicrobial Activity of Romanian Propolis and Antibiotics against Escherichia coli Isolated from Bovine Mastitis

2015 ◽  
Vol 43 (2) ◽  
pp. 327-334 ◽  
Author(s):  
Mihaela NICULAE ◽  
Laura STAN ◽  
Emoke PALL ◽  
Anamaria Ioana PAȘTIU ◽  
Iulia Maria BALACI ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Bovine Mastitis ◽  
Antibacterial Properties ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Ethanolic Extracts

The study was aimed to characterize the chemical composition and the antimicrobial activity of Romanian propolis ethanolic extracts (EEP) against antibiotic-sensitive and antibiotic-resistant E. coli strains isolated from bovine mastitis. The preliminary antimicrobial screening was performed by a disk diffusion method, followed by determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) based on broth microdilution assay; further, the synergistic action of propolis with antimicrobial drugs was assessed by a disk diffusion method on agar containing subinhibitory concentrations of propolis. For the chemical characterisation of EEP, the flavonoids (flavones/flavonols, flavanones/dihydroflavonols) and total phenolics were evaluated by spectrophotometric methods. The phenolic compounds of these extracts were also determined using HPLC. The results indicated for Romanian propolis ethanolic extracts the typical poplar composition profile with flavonoids and phenolic acids as main biological active compounds, with chromatographic analysis data confirmed also spectrophotometrically. In addition, positively correlated with the chemical composition, a strong antimicrobial efficacy was exhibited towards E. coli strains, along with interesting synergistic interaction with antibiotics that can be further investigated to obtain propolis-based formulation with antibacterial properties. Subsequent in vitro and in vivo studies evaluating the safety and efficacy are intended to consider propolis in veterinary therapeutic protocols.

scholarly journals Screening of antimicrobial activity of aqueous and ethanolic extracts of some medicinal plants from Cameroon and assessment of their synergy with common antibiotics against multidrug-resistant uropathogenic bacteria.

2021 ◽  
Author(s):  
Mbarga Manga Joseph Arsene ◽  
Podoprigora Irina Viktorovna ◽  
Anytoulou Kitio Linda Davares ◽  
Mouafo Hippolyte Tene ◽  
Irma Aurelia Monique Manga ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Klebsiella Oxytoca ◽  
Diffusion Method ◽  
Distilled Water ◽  
Strong Activity ◽  
E Coli ◽  
Ethanolic Extracts ◽  
Uropathogenic Bacteria

Background and aim: The Cameroonian flora abounds in plants with multidimensional therapeutic virtues which can play an important role in the fight against resistance to antibiotics and the search for new antimicrobials. This study aimed to screen the antimicrobial potential of aqueous and ethanolic extracts of thirteen (13) samples (Bark, leaf, seed) of eight (8) plants from Cameroon against 3 reference pathogens and to evaluate their synergy with conventional antibiotics against eleven (11) multiresistant uropathogenic (MRU) bacteria. Method: Bioactive compounds were extracted from leaves of Leucanthemum vulgare, Cymbopogon citratus (DC.) Stapf, Moringa oleifera Lam and Vernonia amygdalina Delile; barks of Cinchona officinalis and Enantia chlorantha Oliv; barks and seeds of Garcinia lucida Vesque and leaves and seeds of Azadirachta indica (Neem) using distilled water and ethanol as solvents. The extracts were tested against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 and Candida albicans 10231 using the well diffusion method and the microdilution method. The synergistic effect was assessed (using disc diffusion method and the checkerboard method) against MRU bacteria namely Achromobacter xylosoxidans 4892, Citrobacter freundii 426, Enterococcus avium 1669, Escherichia coli 1449, Klebsiella oxytoca 3003, Kocuria rizophilia. 1542, Moraxella catarrhalis 4222, Morganella morganii 1543, Pseudomonas aeruginosa 3057, Staphylococcus aureus 1449 and Streptococcus agalactiae 3984. Results: We found that distilled water extracted a more important mass of phytochemical compounds (7.9-21.2%) compared to ethanol (5.8-12.4%). Except C. officinalis and G. lucida leaves, the rest of extracts were active with inhibition diameters (ID) ranging from 5 to 36 mm. Both ethanolic (EE) and aqueous extract (AE) of E. chloranta bark (ECB) were the most active against all pathogens with the mean ID of 17 and 36 mm vs S. aureus ATCC 6538, 23 and 14 mm vs E. coli ATCC 25922 and 36 and 19 mm vs C. albicans ATCC 10231. Only the EE of E. chloranta bark (ECB) had a strong activity against all the microorganisms tested (MIC <2 mg / ml); L. vulgare leaves (LVL) and G. lucida seed (GLS) had moderate (average MIC of 8 mg/ml) while all other extracts showed very weak antimicrobial activity. In addition, the fractional inhibitory concentration (FIC) ranged from 0.125 to 0.750. No antagonism (FIC> 4) or indifference (1≤ FIC≤4) was noted between the extracts and the antibiotics, but the best synergies were found with ECB which well-modulated Kanamycin (FIC = 0.125 against S. aureus and 0.250 against E. coli), nitrofurantoin (FIC = 0.250 against S. aureus and 0.188 against E. coli) and ampicillin (FIC = 0.125 against E. coli). Similarly, compared to other extracts, ECB, LVL and GLS also well-modulated ampicillin, ceftazidime, tetracycline, nitrofurantoin, and trimethoprim against all the above-mentioned resistant uropathogenic bacteria with important increase in fold area (IFA). Conclusion: This study show that E. chlorantha bark, L. vulgare leaves G. lucida seed, have good antimicrobial activity against both bacteria (Gram positive and Gram negative) and fungi (C. albicans); and should be more investigated for their possible use to the fight against MDR and MRU microorganisms.

scholarly journals Synergistic antimicrobial activity of crude ethanolic extracts of garlic and neem leaves against bovine mastitis pathogens: an in vitro assay

2017 ◽  
Vol 6 (9) ◽  
pp. 2111
Author(s):  
Makhulu Edmond Edward ◽  
Nyaga Nderitu Samuel ◽  
Wambugu Stanley ◽  
Areba Gerald
Keyword(s):  
Control Strategies ◽  
Bovine Mastitis ◽  
Vitro Assay ◽  
E Coli ◽  
Inhibition Concentration ◽  
Neem Leaves ◽  
Ethanolic Extracts ◽  
Zones Of Inhibition ◽  
Herbal Plants

Background: Treatment of bovine mastitis (BM) is highly expensive necessitating better and affordable control strategies. Synergy of plant extracts against microbes associated with BM stand unexploited. Therefore, this study sought to evaluate the sensitivity of major bovine mastitis bacteria- Staphylococcus aureus and Escherichia coli to combination of two traditional herbal plants, garlic and neem, widely used in East Africa.Methods: Maceration and soxhlet processes were used to obtain crude ethanolic extracts of neem extracts (NE) and garlic (GE) respectively. The extracts were used to determine Minimum Inhibition Concentration (MIC) against test pathogens. Synergy was investigated by combining the two extracts at different concentrations and determining colony forming units per liquid medium (CFU/ml) using turbidity as a measure of inoculum growth.Results: NE was effective against S. aureus at 200µg/ml and 100µg/ml (average zone of inhibitions were 11±0.5774 mm and 10±0.5774 mm respectively). E. coli was resistant to NE. GE was effective against S. aureus at 200µl/ml and 100µl/ml (average zones of inhibition were 14±0.8819 mm and 12±0.8819 mm respectively). GE was effective against E. coli only at 200µl/ml with an average zone of inhibition of 11±0.5774 mm.Conclusions: There was synergy when garlic MIC was combined with NE MIC. Increase in concentration of GE above its MIC increased the rate of decrease of the CFU. No significant change was shown when NE concentration was increased above its MIC. The combination of NE and GE can have additive effect in treatment S. aureus mastitis while GE can supplement NE resistance.

Antimicrobial Activity of Leaves Extracts of Tamarindus indica L. (Magyee)

2019 ◽  
Vol 31 (2) ◽  
pp. 125-130
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Aqueous Extracts ◽  
Tamarindus Indica ◽  
E Coli ◽  
Ethanolic Extracts ◽  
And Staphylococcus Aureus

This study aimed to determine antimicrobial activity of extracts of Tamarindus indica L. leaves (Magyee). It was the laboratory-based quasi experimental study design. Study sites were the University of Pharmacy (Mandalay and Yangon) and the Department of Medical Research (Yangon). The leaves of Tamarindus indica L. were extracted with petroleum ether, ethanol and distilled water by successive Soxhlet extraction method. Physicochemical and phytochemical studies were conducted according to reference analytical procedures. In vitro antimicrobial activity of three extracts of Tamarindus indica L. leaves were studied on control strains of bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae) by agar disc diffusion method. Phytochemical analysis of Tamarindus indica L. leaves showed the presence of alkaloids, amino acids, carbohydrates, flavonoids, glycosides, polyphenol, starches, steroids/terpenoids, tannins, reducing sugars and anthroquinone. Ethanolic and aqueous extracts exhibited significant antibacterial activities against Pseudomonas aeruginosa and Staphylococcus aureus. Both aqueous and ethanolic extracts possessed promising antibacterial activity on Pseudomonas aeruginosa and Staphylococcus aureus. This was an important finding upon pathogenic organisms causing food-borne diseases, skin and soft tissue infection. The petroleum ether extract did not show antibacterial activity against all tested organisms. With ethanolic extracts, the minimum inhibitory concentrations (MIC) results were 6.25 mg/ml for P. aeruginosa, S. aureus and 25 mg/ml for E. coli. For aqueous extracts, the MIC of P. aeruginosa and S. aureus were 12.5 mg/ml and K. pneumoniae was 25 mg/ml, respectively. Oral acute administration of 5000 mg/kg mice body weight of ethanolic and aqueous extracts of Tamarindus indica L. leaves resulted in no mortality. Therefore, the crude extracts from Tamarindus indica L. leaves had antimicrobial activity against K. pneumoniae, P. aeruginosa, S. aureus and E. coli.

scholarly journals Antimicrobial Activity of Some Medicinal Plants from East and Central Part of Nepal

2014 ◽  
Vol 2 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Bimala Subba ◽  
Prakash Basnet
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Proteus Vulgaris ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Ethanolic Extracts ◽  
Inhibitory Activities

To evaluate the antimicrobial activities of extract of eleven plants were examined against four common bacterials. The ethanolic extracts of various plants such as  Cissus repens, Hedyotis scandens, Jatropha curcas, Morus alba, Inula cappa, Equisetum ramosissimum, Osyris wightiana, Alternantheria sessilis and Hibiscus lampas  investigated individually for antimicrobial activity by disc diffusion method. These were investigated against selected species of Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Klebsiella pneumoniae to find the inhibitory activities of the microbes. The ethanolic extract of C. repens showed considerably high activity against P. vulgaris, E. coli and S. aureus than other extracts.DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9697Int J Appl Sci Biotechnol, Vol. 2(1): 88-92

Extracts of Haematoxylon brasiletto Inhibit Growth, Verotoxin Production, and Adhesion of Enterohemorrhagic Escherichia coli O157:H7 to HeLa Cells

2005 ◽  
Vol 68 (7) ◽  
pp. 1346-1351 ◽  
Author(s):  
NORMA HEREDIA ◽  
MARCO ESCOBAR ◽  
CRISTINA RODRÍGUEZ-PADILLA ◽  
SANTOS GARCÍA
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Bacterial Growth ◽  
Hela Cells ◽  
Enterohemorrhagic Escherichia Coli ◽  
Partial Purification ◽  
Escherichia Coli O157 ◽  
Active Fraction ◽  
E Coli ◽  
Ethanolic Extracts

The extracts of 33 plants were evaluated for their effects on growth of Escherichia coli O157:H7 (EHEC). The extract of Haematoxylon brasiletto was the only one that effectively inhibited bacterial growth. The effects of ethanolic extracts of this plant on growth, verotoxin production, and adhesion of E. coli O157:H7 to HeLa cells were determined. The MBC for growth was 4 mg/ml. No verotoxin formation was detected at 1, 2, or 3 mg/ml. Preexposing bacteria and HeLa cells to various concentrations of extracts affected the adhesion between non-EHEC and HeLa cells. Partial purification of the active fraction suggested that polyphenols might play a role in the antimicrobial activity exhibited by H. brasiletto extracts.

scholarly journals Anti Escherichia Coli Activity of Some Medicinal Plants of High Altitude of Nepal

Med Phoenix ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Dipak Bhargava ◽  
Abhilasha Saha ◽  
Keshab Chandra Mondal ◽  
Bijaya Raj Pandit ◽  
Amrullah Shidiki ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
High Altitude ◽  
Plant Extracts ◽  
Clinical Isolates ◽  
Ocimum Sanctum ◽  
Phytochemical Analysis ◽  
E Coli ◽  
Ethanolic Extracts

Background: Plants and plant-based medicaments are the basis of many of the modern pharmaceuticals we use today for our various ailments. The aim of the present study was to find out the bioactive chemical constituents such as flavonoids, alkaloids, tannins, saponins, carbohydrate and to find out the anti E. coli activity of the ethanolic extracts of traditionally used ten medicinal plants of Nepal at an altitude of 1500 ft from above the sea level.Methods: Ethanolic extracts of ten commonly used medicinal plants were analyzed phytochemically and evaluated for their significant antimicrobial activity against the clinical isolates of Escherichia coli. Mean zones of inhibition were calculated for each of the extracts.Results: The results revealed that though all the plants of the high altitude showed some degree of antimicrobial activity, the leaf extract of Syzygium cumini (5.7±0.3 cm), Chromolaena odorata (5.2±0.4 cm), Ocimum sanctum (4.7±0.6 cm) and Justicia adhatoda (3.2±0.3 cm) were most effective against the clinical isolates of E. coli, whereas the other six plant extracts were least effective against the clinical isolates of E. coli. Qualitative phytochemical analysis of the extracts revealed the presence of bioactive components. Seven of the plant extracts contain alkaloids, six of them contain glycosides, four of them contain flavonoids, three of them contain carbohydrate, oil and fats, two of them contain tannins, whereas only one of them contains saponins.Conclusion: The result of this study justified the folkloric usage of the studied plants and concluded that these plants extract have great potential in finding new clinically effective antimicrobial compounds.MED Phoenix Volume (1), Issue (1) July 2016, page: 3-9

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF 3-FORMYL INDOLE BASED SCHIFF BASES

2018 ◽  
Vol 7 (6) ◽  
Author(s):  
Singh Gurvinder ◽  
Singh Prabhsimran ◽  
Dhawan R. K.
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Analysis ◽  
Schiff Bases ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Fungal Strain ◽  
Bacterial Strains ◽  
Standard Drug ◽  
Gram Negative ◽  
E Coli

In order to develop new antimicrobial agents, a series of 3-formyl indole based Schiff bases were synthesized by reacting 3-formyl indole(indole-3-carboxaldehyde) with substituted aniline taking ethanol as solvent. The reaction was carried in the presence of small amount of p-toluene sulphonic acid as catalyst.All the synthesized compounds were characterized by IR, 1H-NMR spectral analysis. All the synthesized compounds were evaluated for antimicrobial activity against two gram positive bacterial strains (B. subtilisand S. aureus) and two gram negative bacterial strains (P. aeruginosaand E. coli) and one fungal strain (C. albicans). All the synthesized compounds were found to have moderate to good antimicrobial activity. The  standard drug amoxicillin, fluconazole were used for antimicrobial activity. Among the synthesized compounds, the maximum antimicrobial activity was shown by compounds GS04, GS07, GS08 and GS10.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

Antimicrobial Activity of Silver Nanoparticles Prepared Under an Ultrasonic Field

2011 ◽  
Vol 4 (3) ◽  
pp. 1491-1496
Author(s):  
Umadevi M ◽  
Rani T ◽  
Balakrishnan T ◽  
Ramanibai R
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Reduction Method ◽  
Therapeutic Potential ◽  
Chemical Reduction ◽  
Ultrasonic Field ◽  
Great Promise ◽  
Chemical Reduction Method ◽  
E Coli

Nanotechnology has great promise for improving the therapeutic potential of medicinal molecules and related agents. In this study, silver nanoparticles of different sizes were synthesized in an ultrasonic field using the chemical reduction method with sodium borohydride as a reducing agent. The size effect of silver nanoparticles on antimicrobial activity were tested against the microorganisms Staphylococcus aureus (MTCC No. 96), Bacillus subtilis (MTCC No. 441), Streptococcus mutans (MTCC No. 497), Escherichia coli (MTCC No. 739) and Pseudomonas aeruginosa (MTCC No. 1934). The results shows that B. subtilis, and E. coli were more sensitive to silver nanoparticles and its size, indicating the superior antimicrobial efficacy of silver nanoparticles. 

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