scholarly journals Antibiotic Sensitivity and Evaluation of Plasmid Profile of Major Foodborne Pathogens

2016 ◽  
Vol 06 (04) ◽  
pp. 04-09 ◽  
Author(s):  
Ibikunle Ibitayo Anibijuwon ◽  
Ifeoluwa Deborah Gbala ◽  
Jumai Adeola Abioye ◽  
Paul Oluwaseun Ogunlade
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Foodborne Pathogens ◽  
Susceptibility Testing ◽  
Antibiotic Sensitivity ◽  
Plasmid Profile ◽  
Antibiotics Resistance ◽  
Proteus Vulgaris ◽  
Food Borne ◽  
Plasmid Profiling

AbstractThis study revealed the reason behind the antibiotics resistance of isolated food-borne pathogens through their susceptibility testing to various antibiotics of choice. The results of the study revealed that resistance of the bacteria isolates which are Streptococcus sp., Staphylococcus aureus, Proteus vulgaris, Shigella sp., Escherichia coli, Pseudomonas aeruginosa, Bacillus sp. to different antibiotics varies and differs considerably. For instance, Pseudomonas aeruginosa showed resistance to Amoxicillin, Augmentin, Gentamicin and Tetracycline. Staphylococcus sp. isolated showed multiple resistances to Cloxacillin, Erythromycin, Amoxicillin, Augmentin and Gentamicin. Proteus vulgaris showed multiple resistances to five antibiotics in-vitro which are Augmentin, Nitrofurantoin, Amoxicillin, Cotrimoxazole and Nalidixic. The seven isolates were then assayed for plasmid profiling by agarose gel electrophoresis. All the isolates has plasmid with varying sizes of between 9–21kb. Further conjugative study will reveal more reason behind the resistance.

scholarly journals Pseudomonas aeruginosa as a Potential Contaminant of Packed Fresh-Cut Lettuce in a Controlled Atmosphere. The Role of Phenotypes muc+/muc–

2020 ◽  
Vol 11 (2) ◽  
pp. 8716-8724
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Real Time ◽  
Foodborne Pathogens ◽  
Rrna Gene ◽  
Modified Atmosphere ◽  
Fresh Cut ◽  
Time Specific ◽  
Sanitation Systems ◽  
Biofilm Development

In order to shed light on contamination risks along the ready-to-eat chain of fresh commodities by emerging foodborne pathogens, we investigated the biofilm development in vitro of two Pseudomonas aeruginosa strains on fresh-cut lettuce (Lactuca sativa L. var. Iceberg). The experiment was performed employing a floating bioreactor system where modified atmosphere package conditions were mimicked, and fresh-cut lettuce disks of 2 cm2 were put into contact with a 106 CFU/mL of a phenotypic mucoid P. aeruginosa phenotype (muc+) or a non-mucoid one (muc-). Following a simulated 2-day refrigerated-shelf quantitative Real-Time PCR, designed on a target gene region of the 16S rRNA gene, defined the different muc phenotypes behavior on biofilm in lettuce phyllo-plane. Between the two strains, a development difference of nearly 1.0 log CFU/cm2 occurred, with the muc+ phenotype being the most settled and adherent. This result clearly showed a distinct contamination risk according to P. aeruginosa phenotype and the need to develop real-time, specific, fast, and easy to use detection protocols along with specific sanitation systems for modified atmosphere package ready-to-eat commodities.

scholarly journals Disruption of Cross-Feeding Inhibits Pathogen Growth in the Sputa of Patients with Cystic Fibrosis

mSphere ◽  
2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Jeffrey M. Flynn ◽  
Lydia C. Cameron ◽  
Talia D. Wiggen ◽  
Jordan M. Dunitz ◽  
William R. Harcombe ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Anaerobic Bacteria ◽  
Content Type ◽  
Growth Environment ◽  
Polymicrobial Infections

ABSTRACT A critical limitation in the management of chronic polymicrobial infections is the lack of correlation between antibiotic susceptibility testing (AST) and patient responses to therapy. Underlying this disconnect is our inability to accurately recapitulate the in vivo environment and complex polymicrobial communities in vitro. However, emerging evidence suggests that, if modeled and tested accurately, interspecies relationships can be exploited by conventional antibiotics predicted to be ineffective by standard AST. As an example, under conditions where Pseudomonas aeruginosa relies on cocolonizing organisms for nutrients (i.e., cross-feeding), multidrug-resistant P. aeruginosa may be indirectly targeted by inhibiting the growth of its metabolic partners. While this has been shown in vitro using synthetic bacterial communities, the efficacy of a “weakest-link” approach to controlling host-associated polymicrobial infections has not yet been demonstrated. To test whether cross-feeding inhibition can be leveraged in clinically relevant contexts, we collected sputa from cystic fibrosis (CF) subjects and used enrichment culturing to isolate both P. aeruginosa and anaerobic bacteria from each sample. Predictably, both subpopulations showed various antibiotic susceptibilities when grown independently. However, when P. aeruginosa was cultured and treated under cooperative conditions in which it was dependent on anaerobic bacteria for nutrients, the growth of both the pathogen and the anaerobe was constrained despite their intrinsic antibiotic resistance profiles. These data demonstrate that the control of complex polymicrobial infections may be achieved by exploiting obligate or facultative interspecies relationships. Toward this end, in vitro susceptibility testing should evolve to more accurately reflect in vivo growth environments and microbial interactions found within them. IMPORTANCE Antibiotic efficacy achieved in vitro correlates poorly with clinical outcomes after treatment of chronic polymicrobial diseases; if a pathogen demonstrates susceptibility to a given antibiotic in the lab, that compound is often ineffective when administered clinically. Conversely, if a pathogen is resistant in vitro, patient treatment with that same compound can elicit a positive response. This discordance suggests that the in vivo growth environment impacts pathogen antibiotic susceptibility. Indeed, here we demonstrate that interspecies relationships among microbiotas in the sputa of cystic fibrosis patients can be targeted to indirectly inhibit the growth of Pseudomonas aeruginosa. The therapeutic implication is that control of chronic lung infections may be achieved by exploiting obligate or facultative relationships among airway bacterial community members. This strategy is particularly relevant for pathogens harboring intrinsic multidrug resistance and is broadly applicable to chronic polymicrobial airway, wound, and intra-abdominal infections.

scholarly journals Phage steering of antibiotic-resistance evolution in the bacterial pathogen, Pseudomonas aeruginosa

2020 ◽  
Vol 2020 (1) ◽  
pp. 148-157 ◽  
Author(s):  
James Gurney ◽  
Léa Pradier ◽  
Joanne S Griffin ◽  
Claire Gougat-Barbera ◽  
Benjamin K Chan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Bacterial Pathogen ◽  
Antibiotic Sensitivity ◽  
Resistant Bacteria ◽  
Phage Resistance ◽  
Antibiotic Resistant ◽  
Trade Off

Abstract Background and objectives Antimicrobial resistance is a growing global concern and has spurred increasing efforts to find alternative therapeutics. Bacteriophage therapy has seen near constant use in Eastern Europe since its discovery over a century ago. One promising approach is to use phages that not only reduce bacterial pathogen loads but also select for phage resistance mechanisms that trade-off with antibiotic resistance—so called ‘phage steering’. Methodology Recent work has shown that the phage OMKO1 can interact with efflux pumps and in so doing select for both phage resistance and antibiotic sensitivity of the pathogenic bacterium Pseudomonas aeruginosa. We tested the robustness of this approach to three different antibiotics in vitro (tetracycline, erythromycin and ciprofloxacin) and one in vivo (erythromycin). Results We show that in vitro OMKO1 can reduce antibiotic resistance of P. aeruginosa (Washington PAO1) even in the presence of antibiotics, an effect still detectable after ca.70 bacterial generations in continuous culture with phage. Our in vivo experiment showed that phage both increased the survival times of wax moth larvae (Galleria mellonella) and increased bacterial sensitivity to erythromycin. This increased antibiotic sensitivity occurred both in lines with and without the antibiotic. Conclusions and implications Our study supports a trade-off between antibiotic resistance and phage sensitivity. This trade-off was maintained over co-evolutionary time scales even under combined phage and antibiotic pressure. Similarly, OMKO1 maintained this trade-off in vivo, again under dual phage/antibiotic pressure. Our findings have implications for the future clinical use of steering in phage therapies. Lay Summary: Given the rise of antibiotic-resistant bacterial infection, new approaches to treatment are urgently needed. Bacteriophages (phages) are bacterial viruses. The use of such viruses to treat infections has been in near-continuous use in several countries since the early 1900s. Recent developments have shown that these viruses are not only effective against routine infections but can also target antibiotic resistant bacteria in a novel, unexpected way. Similar to other lytic phages, these so-called ‘steering phages’ kill the majority of bacteria directly. However, steering phages also leave behind bacterial variants that resist the phages, but are now sensitive to antibiotics. Treatment combinations of these phages and antibiotics can now be used to greater effect than either one independently. We evaluated the impact of steering using phage OMKO1 and a panel of three antibiotics on Pseudomonas aeruginosa, an important pathogen in hospital settings and in people with cystic fibrosis. Our findings indicate that OMKO1, either alone or in combination with antibiotics, maintains antibiotic sensitivity both in vitro and in vivo, giving hope that phage steering will be an effective treatment option against antibiotic-resistant bacteria.

scholarly journals Modulation of antibiotic sensitivity and biofilm formation in Pseudomonas aeruginosa by interspecies diffusible signal factor analogues

2018 ◽  
Author(s):  
Shi-qi An ◽  
Julie Murtagh ◽  
Kate B. Twomey ◽  
Manoj K. Gupta ◽  
Timothy P. O’Sullivan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Unsaturated Fatty Acids ◽  
Antibiotic Sensitivity ◽  
Opportunistic Pathogen ◽  
Lead Compounds ◽  
Infection Models ◽  
Diffusible Signal Factor ◽  
Input Domain

ABSTRACTThe opportunistic pathogen Pseudomonas aeruginosa can participate in inter-species communication through signaling by cis-2-unsaturated fatty acids of the diffusible signal factor (DSF) family. Sensing these signals involves the histidine kinase PA1396 and leads to altered biofilm formation and increased tolerance to various antibiotics. Here, we show that the membrane-associated sensory input domain of PA1396 has five trans-membrane helices, two of which are required for DSF sensing. DSF binding is associated with enhanced auto-phosphorylation of PA1396 incorporated into liposomes. Further, we examined the ability of synthetic DSF analogues to modulate or inhibit PA1396 activity. Several of these analogues block the ability of DSF to trigger auto-phosphorylation and gene expression, whereas others act as inverse agonists reducing biofilm formation and antibiotic tolerance, both in vitro and in murine infection models. These analogues may thus represent lead compounds for novel adjuvants to improve the efficacy of existing antibiotics.

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF MICRO PROPAGATED CALLUS OF CURUULIGO ORCHIODES (BLACK MUSILLI) AGAR WELL DIFFUSION AND MINIMUM INHIBITORY CONCENTRATION (MIC)

2017 ◽  
Vol 9 (3) ◽  
pp. 75
Author(s):  
Rajanikanth Garapati ◽  
N. Ramesh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Bacillus Cereus ◽  
Proteus Vulgaris ◽  
Methanol Extracts ◽  
Tract Infections

Objective: In vitro investigated the potential of methanol extracts of micro-propagated C. orchiodes in the antimicrobial property against the three gram-negative bacteria, two gram-positive and one fungal filament.Methods: The micro propagated callus methanol extract was examined against Escherichia coli, Proteus vulgaris, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans. The zone of inhibitions are determined at 10 mg/ml concentration of methanol extracts of callus on agar well plate and MIC against tested microorganism.Results: The highest antibacterial activity recorded in Staphylococcus aureus Bacillus cereus and followed by Candida albicans. Antibacterial activity of leaf extracts of A. reticulata was also significant against the tested microorganisms Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa compared to ciprofloxacin.Conclusion: Based on the above observations, these extracts were further evaluated for their effect on microorganisms causing infections like typhoid fever, urinary tract infections, septicemia, toxic shock syndrome, skin infection, nosocomial infection, arthritis and diarrhoea. The results also suggest that these plants serve a therapeutic purpose in the treatment bacterial infections.

scholarly journals Establishment and Application of a Dual TaqMan Real-Time PCR Methodfor Proteus mirabilis and Proteus vulgaris

2020 ◽  
Vol 69 (3) ◽  
pp. 293-300
Author(s):  
RUI YANG ◽  
GUOYANG XU ◽  
XIAOYOU WANG ◽  
ZHICHU QING ◽  
LIZHI FU
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Proteus Mirabilis ◽  
Real Time Pcr ◽  
Proteus Vulgaris ◽  
Opportunistic Bacteria ◽  
Food Borne ◽  
Colony Forming Units ◽  
Pcr Method ◽  
Pure Bacterial Cultures

Proteus species are common opportunistic bacteria and foodborne pathogens. The proper detection of Proteus can effectively reduce the occurrence of food-borne public health events. Proteus mirabilis and Proteus vulgaris are the two most important pathogens in the Proteus genus. In this study, a dual TaqMan Real-Time PCR method was established to simultaneously detect and distinguish P. mirabilis and P. vulgaris in samples. The method exhibited good specificity, stability, and sensitivity. Specifically, the minimum detection concentrations of P. mirabilis and P. vulgaris in pure bacterial cultures were 6.08 × 102 colony forming units (CFU)/ml and 4.46 × 102 CFU/ml, respectively. Additionally, the minimum detectable number of P. mirabilis and P. vulgaris in meat and milk was 103 CFU/g. In addition, the method can be used to distinguish between strains of P. mirabilis and P. vulgaris within two hours. Overall, it is a sensitive, easy-to-use, and practical test for the identification and classification of Proteus in food.

scholarly journals Comparative study of in vitro activities of polymyxin B commercial products on Pseudomonas aeruginosa isolated from hospitalized patients

2021 ◽  
Vol 62 (3) ◽  
pp. 270-279
Author(s):  
Rezvan Goodarzi ◽  
Farhad Farahani ◽  
Mahdane Roshani ◽  
Mohammad Taheri ◽  
Babak Asghari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Infections ◽  
Susceptibility Testing ◽  
Renal Toxicity ◽  
Polymyxin B ◽  
Microbiological Activity ◽  
Commercial Products ◽  
Gram Negative ◽  
In Vitro Antibacterial Activity

Introduction: Polymyxin B has been applied as one of the last-resort antibiotics for the treatment of multidrug resistance among Gram-negative bacterial infections. Due to side effects such as renal toxicity, the use of polymyxin is associated with limitations. The present study evaluates in vitro antibacterial activity of a number of polymyxin B commercial products against Pseudomonas aeruginosa. Methods: This study included 63 non-duplicated P. aeruginosa isolates examined for in vitro polymyxin B susceptibility testing using the following powder disks: polymyxin B sulfate, otosporin, Poly-Mxb, and Myxacort. MIC50 and MIC90 have also been identified for polymyxin B antibiotics. Results: Myxacort had functional activity against most P. aeruginosa isolates, and only seven isolates had a relatively high MIC. The activities of Poly-MXb and Myxacort were the same as otosporin. Conclusions: Our findings revealed that the national generic polymyxin B product (Myxacort), and two external products (Otosporin, Poly-MXb) are similar in terms of microbiological activity.

scholarly journals 1222. Ceftolozane-Tazobactam Heteroresistance in Cystic Fibrosis Related Pseudomonas aeruginosa Infections

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S701-S701
Author(s):  
James Sanders ◽  
Marguerite Monogue ◽  
David E Greenberg ◽  
Christine A Pybus ◽  
Andrew E Clark
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Susceptibility Testing ◽  
Population Analysis ◽  
Fold Change ◽  
Small Subset ◽  
Patient Specimen ◽  
Clinical Responses ◽  
Susceptibility Profiles

Abstract Background Cystic fibrosis (CF) patients are often colonized with Pseudomonas aeruginosa (PSA). During treatment, PSA can develop subpopulations exhibiting variable in vitro antimicrobial susceptibility patterns. Heteroresistance may underlie the reported discordant in vitro results and clinical responses to various antimicrobials. Here, we sought to examine the presence and nature of PSA heteroresistance to ceftolozane-tazobactam (C-T) in isolates originating from CF pulmonary exacerbations. Methods Respiratory cultures from 26 adult CF patients were collected. From each sample, 5-10 PSA colonies were selected. Susceptibility testing was conducted via E-test for C-T, ceftazidime-avibactam (CZA), and imipenem-relebactam (I-R). Polyclonal-heteroresistance (PHR) was defined as the presence of different susceptibility profiles among the colonies that originated from a single patient specimen. Population analysis profile (PAPs) were performed to assess the presence of monoclonal-heteroresistance (MHR), defined as ≥ 4 fold change in the C-T MIC from a single colony over 24-48 hours. Results 246 PSA isolates from 26 adult CF patients were included. The C-T MIC50 and MIC90 were 1/4 and ≥ 256/4 µg/mL, respectively (Figure 1). Sixteen of the 26 patients (62%) demonstrated ≥ 2 fold change in C-T MIC between isolates from the same culture. Of these 16 isolates, the fold change in C-T MIC was >2 fold for 7 isolates (27%) and resulted in a susceptibility interpretation change in 6 of the isolates (23%). Of the 32 isolates that underwent PAP testing, 7 grew on MH plates at 2-fold the C-T MIC concentration. One isolate, PSA 1311, demonstrated growth on PAPs up to 4 fold the MIC (16/4 µg/mL) (Figure 2). Figure 1. Ceftolozane-tazobactam, ceftazidime-avibactam, and imipenem-relebactam MIC distributions against PSA isolates from 26 adult CF patients Figure 2. Monoclonal heteroresistance to C-T (PSA 1311 [C-T MIC 1/4 µg/mL] PAPs at 2, 4, 6, and 8-fold the C-T MIC). Conclusion Susceptibilities to C-T and CZA were similar across our CF PSA isolates. Comparatively, I-R retained better in vitro potency. C-T PHR exists among PSA isolates in the majority of our CF patients. Approximately 25% of these PHR isolates resulted in susceptibility interpretation changes supporting concerns surrounding the utility of traditional susceptibility testing methodology for CF isolates. These data suggest MHR also exists, albeit rare in this small subset. Additional data are needed to better understand these results in clinical context. Disclosures David E. Greenberg, MD, Shionogi (Grant/Research Support)Solenic Medical (Shareholder)

scholarly journals Susceptibility of clinical Enterobacterales and Pseudomonas aeruginosa isolates to ceftazidimeavibactam in Russia: multicenter local laboratory databased surveillance

2021 ◽  
Vol 23 (3) ◽  
pp. 264-278 ◽  
Author(s):  
Mikhail V. Edelstein ◽  
Elena Yu. Skleenova ◽  
Ivan V. Trushin ◽  
Alexey Yu. Kuzmenkov ◽  
Alexey А. Martinovich ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Susceptibility Testing ◽  
Vitro Activity ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Case Report Form ◽  
In Vitro Activity ◽  
Disk Diffusion Method ◽  
Online Platform

Objective. To assess the in vitro activity of ceftazidime-avibactam against clinical Enterobacterales and Pseudomonas aeruginosa isolates in various regions of Russia based on results of local susceptibility testing by disk diffusion method. Materials and Methods. Overall, 160 laboratories located in 61 Russian cities participated in this surveillance during 2018-2020. All consecutive clinical isolates of Enterobacterales and Pseudomonas aeruginosa in each participating laboratory were included in the study. Ceftazidime-avibactam susceptibility testing was done by disc-diffusion method in accordance with current EUCAST recommendations. Susceptibility data for carbapenems and III-IV generation cephalosporins, as well as results of carbapenemases detection, were also reported, if available. All the data were recorded in electronic case report form developed on the OpenClinica online platform (www.openclinica.com). Data analysis and reporting were done using AMRcloud online platform (https://amrcloud.net/). Results. In total, we received information on antimicrobial susceptibility of 22,121 isolates, including 17,456 (78.9%) Enterobacterales and 4,665 (21.1%) P. aeruginosa. Less than 9% of Enterobacterales isolates were resistant to ceftazidime-avibactam. At the same time rates of resistance to ceftazidime, cefotaxime, cefepime, ertapenem, imipenem, and meropenem were 54.1%, 58.9%, 59.4%, 41.4%, 23.9%, and 21.3%. Among Enterobacterales the highest level of resistance to ceftazidime-avibactam was detected in K. pneumoniae (16.5%), lowest – in E. coli (2.1%). Some increase of resistance to ceftazidimeavibactam was noted during the study – from 7.8% in 2018-2019 to 9.6% in 2020 (p = 0.0001). Rate of resistance to ceftazidime-avibactam in P. aeruginosa was 33.1%. At the same time rates of resistance to ceftazidime, cefepime, imipenem, and meropenem were 51.1%, 54.5%, 50%, and 47.3%. During the study there was statistically significant decrease in resistance to ceftazidime-avibactam in P. aeruginosa (p = 0.0001). Resistance rates for all beta-lactams for both Enterobacterales and P. aeruginosa were higher in nosocomial isolates than in community-acquired isolates. Conclusions. Ceftazidime-avibactam demonstrated significantly higher in vitro activity against Enterobacterales and P. aeruginosa Russian clinical isolates comparing with commonly used carbapenems and extended spectrum cephalosporins. Access for all study data available at the AMRcloud online platform (https://amrcloud.net/ru/project/cazavi-1-2/).

scholarly journals ДОКЛІНІЧНЕ ДОСЛІДЖЕННЯ НАНОЧАСТИНОК ФЕРУМУ

2021 ◽  
pp. 17-24
Author(s):  
Y. S. Stravskyy ◽  
L. Ya. Fedoniuk ◽  
O. M. Yarema ◽  
E. І. Skyba ◽  
L. S. Reznichenko
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Salmonella Typhimurium ◽  
Proteus Mirabilis ◽  
Shigella Sonnei ◽  
Proteus Vulgaris

Вступ. Доклінічне вивчення лікарських препаратів – невід’ємна частина процесу створення лікарського засобу. Доклінічне дослідження є найбільш тривалим та відповідальним етапом розробки лікарського засобу, який вимагає особливих підходів до планування і забезпечення якості при плануванні вимірювальних експериментів, проведенні випробування та оцінки його результатів. Мета дослідження – визначити біобезпечність, гостру токсичність, протимікробну та фунгіцидну дії наночастинок Феруму. Методи дослідження. Біобезпечність синтезованої субстанції наночастинок у тестах in vitro визначали з використанням показників цитотоксичності, мутагенності, молекулярно-генетичного (показник генотоксичності), фізіологічного (стан мікрофлори шлунково-кишкового тракту людини) та біохімічних (ATФ-aзна і лактатдегідрогеназна активність) маркерів. Протимікробну дію нуль-валентного Феруму (Fe0NP) щодо тест-штамів мікроорганізмів визначали методом серійних розведень у бульйоні відповідно до Методичних вказівок 4.2.1890-04, 2004. Використовували такі тест-штами мікроорганізмів, як Salmonella typhimurium, Shigella sonnei, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Proteus mirabilis, Candida albicans, із колекції Державного науково-контрольного інституту біотехнології і штамів мікро­організмів. Результати й обговорення. Синтезовані наночастинки є частинками Fe0NP. Взаємодія синтезованих наночастинок Феруму з тестовими еукаріотичними клітинами не призводила до появи первинних ДНК‑ушко­джень порівняно з впливом N-нітрозометилсечовини, яка є відомим генотоксикантом. Синтезовані наночастинки характеризувались як біобезпечні у тестах на мутагенність з використанням поліхроматофільних еритроцитів кісткового мозку тварин. Аналіз показав, що експериментальна субстанція Fe0NP у досліджуваному концентраційному діапазоні проявила помірну протимікробну активність у тестах in vitro відносно як грамнегативних (S. typhimurium, S. sonnei, P. aeruginosa, P. vulgaris, P. mirabilis), так і грампозитивних (S. aureus) мікроорганізмів. Однак гриби Candida albicans виявилися нечутливими до наночастинок Феруму в досліджуваних концентраціях. Висновки. Фізико-хімічна характеристика й оцінка критеріїв біобезпечності в тестах in vitro та in vivo свідчать про те, що синтезованим сферичним наночастинкам нуль-валентного Феруму властивий низький рівень потенційної небезпеки: виявлено відсутність генотоксичної, цитотоксичної, мутагенної дій, негативного впливу на ключові біохімічні параметри і загальний фізіологічний стан живого організму. Це дозволяє рекомендувати синтезовану субстанцію наночастинок Феруму для подальших досліджень з метою їх застосування як потенційної біологічно активної субстанції.

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