Pseudobasophilia as a Screening Tool in Dengue: A Single Center Study

Abstract Objectives Proper serological testing for the definite diagnosis of dengue is costly and may not be easily available in a resource-limited setting. Hematological parameters can help in the early identification of dengue cases. This study aims to evaluate the occurrence and utility of pseudobasophilia in identifying dengue-affected patients. Materials and Methods This retrospective cross-sectional study included 1,304 dengue cases confirmed by serology and 1,044 dengue serology negative acute febrile illness cases as controls. Complete blood count (CBC) values of the first EDTA (ethylenediamine tetraacetic acid) blood sample from automated hematology analyzers were reviewed. The hematological parameters in the dengue and control groups were compared and the variation of these parameters with the day of fever was also analyzed. Statistical Analysis Mann-Whitney’s test, Kruskal-Wallis test, and Fisher’s exact test were used for statistical analysis. A p-value < 0.05 was considered statistically significant for all tests. Results There was a statistically significant variation between dengue cases and controls for hematocrit, platelet count, mean platelet volume, total white blood cell count, and absolute basophil count. The dengue group had a higher hematocrit from day 2 to day 10, platelet count ≤ 100,000/µL from day 4 to day 9, higher mean platelet volume from day 2 to day 7, leucopenia from day 3 to day 5, and higher absolute basophil count from day 2 to day 10. Interestingly, pseudobasophilia was seen in 533 (40.87%) of dengue cases and only 3 (0.28%) of the controls. Pseudobasophilia was also observed to have an increasing trend to the day of fever. Conclusion Pseudobasophilia along with other CBC parameters is useful and cost effective for the early identification of dengue. This can prompt early investigations and supportive treatment leading to improved clinical outcomes.

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Evaluation of Mean Platelet Volume (MPV) in Pre-Eclamptic and Normotensive Women in 3rd Trimester of Gestation

10.53350/pjmhs211562119 ◽

2021 ◽

Vol 15 (6) ◽

pp. 2119-2121

Author(s):

Salza Zainab ◽

Armish Saeed ◽

Shazia Awan ◽

Sehrish Raja ◽

Faiza Khanum ◽

...

Keyword(s):

Blood Pressure ◽

Mean Platelet Volume ◽

Complete Blood Count ◽

Cost Effective ◽

Cross Sectional Study ◽

P Value ◽

Platelet Volume ◽

Cross Sectional ◽

The Mean ◽

Automated Hematology Analyzer

Objective: To determine the mean platelet volume (MPV) in pre-eclamptic and normotensive women presenting in 3rd trimester of pregnancy. Study Design: Descriptive Cross-sectional study. Place and Duration: Department of Obstetrics & Gynecology, Multan Medical and Dental College/Ibn-e-Sina Hospital, Multan during from 29-May-2019 to 28-Nov-2019. Methodology: 246 pregnant females were included in this study. Patients were assessed for pre-eclampsia those who were history of blood pressure >140 mmHg and 90 mmHg diastolic as seen on examination on three consecutive readings. Data of maternal hemogram taken at presentation was recording using an automated hematology analyzer. mean platelet volume (MPV) was recorded at completion of 36th week of gestation. Results: Mean age of patients included in this study was 34.22±5.02 years. Mean body mass index (BMI) of patients was 24.58±4.65 kg/m2. Mean parity was 2.63±0.78. Mean blood pressure of patients was 128.37±15.06 mmHg. The mean platelet volume in patients with normotensive pregnancy was 9.27±1.31 fL versus 12.49±1.51 fL in patients pre-eclampsia. This difference was statistically significant with p-value of <0.0001. Conclusion: As a biomarker for preeclampsia severity, MPV is a valuable tool. In a clinical context, MPV is regularly acquired during a complete blood count, making it a potentially cost-effective prediction tool for evaluating pre-eclampsia Keywords: Pregnancy, Pre-eclampsia, Mean Platelet Volume (MPV)

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Studies on Reference Intervals for Platelet Counts in Three Cities in China and one in Japan

Thrombosis and Haemostasis ◽

10.1055/s-0037-1613162 ◽

2002 ◽

Vol 88 (07) ◽

pp. 111-114 ◽

Cited By ~ 3

Author(s):

Changgeng Ruan ◽

Yun Wu ◽

Tokuhiro Okada ◽

Shigemi Motoi ◽

Tamiaki Kondo ◽

...

Keyword(s):

Platelet Count ◽

Mean Platelet Volume ◽

Healthy Subjects ◽

Hematological Parameters ◽

Reference Interval ◽

Reference Intervals ◽

Sichuan Province ◽

Jiangsu Province ◽

Platelet Volume ◽

Platelet Counts

SummaryHematological parameters including platelet counts, etc. were determined in 1,140 healthy subjects living in four cities: Suzhou (Jiangsu Province), Chengdu (Sichuan Province) and Harbin (Heilongjang Province) in China, and Kobe in Japan. Then, the reference intervals for platelet counts were calculated and compared. The reference interval for platelet count of subjects aged between 18 and 60 years was 60-259 × 109/L in Suzhou and 52–202 × 109/L in Chengdu, and subjects with platelet counts of 100 × 109/L or less accounted for about 30% of the subjects examined in these cities. The reference intervals in Harbin and Kobe were within the range of 150–350 × 109/L, and no subject having a platelets count of 100 × 109/L or less was detected. Mean platelet volume (MPV) determined concurrently was negatively correlated with platelet count, and the reference intervals for MPV in Chengdu and Suzhou were higher than those in Harbin and Kobe.

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Study the relationship between nasal polyps, platelet count, mean platelet volume, neutrophil/lymphocyte ratio in patients attending otolaryngology OPD in a tertiary care teaching hospital, West Bengal

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20170453 ◽

2017 ◽

Vol 3 (2) ◽

pp. 228

Author(s):

Manuprita Sharma ◽

Santosh Raman ◽

Bidhan Ray ◽

Rupali Verma Bagga ◽

Ramesh Kumar Sahu ◽

...

Keyword(s):

Platelet Count ◽

Nasal Polyp ◽

Mean Platelet Volume ◽

Complete Blood Count ◽

Tertiary Care ◽

Control Group ◽

Blood Cell Count ◽

Platelet Volume ◽

Distribution Width ◽

The Relationship

Background: Nasal obstruction is a common cause of marked nasal septal deviation. It is related strongly with hypoxia. Hypoxic conditions increase mean platelet volume levels. Many studies in literature point out that inflammation related to nasal polyp is mostly dependent on eosinophils and their inflammatory products. Beside eosinophils, platelets may have a role in nasal polyp development. Platelets are involved in hemostasis, tissue repairing and inflammation. Recently, mean platelet volume (MPV) was recognized as a simple inflammatory marker in the inflammatory disease. This study investigated the relationship of nasal polyps with MPV (mean platelet volume), platelet count and NLR (neutrophil to lymphocyte ratio).Methods: The cross-sectional observational study we investigated CBC (complete blood count) parameters of the patients that consulted ENT clinic in a tertiary care teaching hospital, Haldia because of nasal polyp. About 76 patients and 42 controls were included in this study. In all groups, WBC (white blood cell count), RBC (red blood cell count), RDW (red cell distribution width), platelet count, MPV, PDW (platelet distribution width) and NLR parameters from CBC (complete blood count) were compared between patients and controls. Results: Mean WBC values were 6.89±1.33×103/μL in patients with NPs, vs. 7.11±2.32×103/μL in the control group. Mean haemoglobin (Hb) values were 13.19±1.34 g/dL in patients with NPs, vs. 14.1±1.67 g/dl in the control group. Mean MPV values were 9.11 ± 1.08 fL in patients with NPs, vs. 8.32±0.53 fL in the control group. Mean PLT values were 232.38 ± 39.97×103/μL in patients with NPs, vs. 271.44±45.14×103 in the control group. MPV was higher in nasal polyp patients, whereas platelet count was higher in controls. The difference was significant for the mean absolute neutrophil, platelet count, and lymphocyte counts, NLR, PDW and MPV values between the study group and the control group. Conclusions: Platelet count and NLR were significantly lower in patients than the controls, whereas MPV was significantly higher in nasal polyp patients.

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A HOSPITAL BASED COMPARATIVE STUDY OF SERUM MEAN PLATELET VOLUME IN ISCHEMIC STROKE PATIENTS.

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v3i9.554 ◽

2019 ◽

Vol 3 (9) ◽

Author(s):

Dr. Atul Baid ◽

Dr. Chhavi Raman Baid

Keyword(s):

Platelet Count ◽

Ischemic Stroke ◽

Mean Platelet Volume ◽

Normal Subjects ◽

Rank Test ◽

P Value ◽

Modified Rankin Scale ◽

Platelet Volume ◽

Laboratory Findings ◽

Significant Difference

Objectives: This study was evaluated the association of serum means platelet volume, functional outcome and various parameters in patients of ischemic stroke. Methods: Detail history clinical examinations and relevant investigations were performed to all subjects. Lab parameters included as platelets counts, mean platelet volume and others were performed. The diagnosis of ischaemic stroke was made clinically with the evidence of acute lesions (infarct) confirmed by brain CT or MRI within the first 24 h of presentation of symptoms. Each patient condition was assessed by modified Rankin Scale. Results: Data was analyzed using SPSS version 26 software. Related-Samples Wilcoxon Signed Rank Test was applied. Mean and standard deviation were calculated. P value was taken less than or equal to 0.05 for significant differences (p ≤ 0.05). Conclusions: There was no significant difference seen in platelet count of ischemic stroke cases with control. Mean platelet volume was significantly higher in ischemic stroke cases than normal subjects. Majorities of ischemic stroke cases had moderate disability, required some help but able to walk without assistance. MPV was higher in ischemic stroke cases that had higher Modified Rankin scale. Hence, serum MPV can be used as meaningful laboratory findings for early detection of ischemic stroke. Key words: Ischemic stroke, mean platelet volume, platelet count, modified Rankin score

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Mean Platelet Volume and Neutrophil-to-Lymphocyte Ratio May Be Used as Predictors in Febrile Seizures

Journal of Pediatric Infectious Diseases ◽

10.1055/s-0038-1668534 ◽

2018 ◽

Vol 13 (04) ◽

pp. 283-286

Author(s):

Fatih Akın ◽

Esra Türe ◽

Hüseyin Çaksen ◽

Dursun Odabaş ◽

Abdullah Yazar

Keyword(s):

Mean Platelet Volume ◽

Febrile Seizure ◽

Complete Blood Count ◽

Febrile Illness ◽

Absolute Lymphocyte Count ◽

Neutrophil To Lymphocyte Ratio ◽

Control Group ◽

Platelet Volume ◽

Lymphocyte Ratio ◽

Study Results

AbstractFebrile seizure (FS) is the most frequent seizure disorder in childhood, associated with rapid onset of high fever. Our study aims are (1) to determine if the levels of mean platelet volume (MPV) and neutrophil-to-lymphocyte ratio (NLR) are risk factors for FS and to (2) assess the usefulness of these markers as predictors to distinguish the subgroups of FS. This prospective study includes children with FS, acute febrile illness (AFI) without seizure, and control group. Complete blood count was performed on all participants. The following data were obtained: white blood cell count (WBC), platelet count, MPV, hemoglobin (Hb), absolute neutrophil count (ANC), absolute lymphocyte count (ALC), and NLR. MPV, WBC, ANC, and NLR were significantly increased in patients with AFI and FS compared with controls (p < 0.05). When patients with FS and AFI were compared, only WBC was increased significantly in patients with FS (p < 0.05). WBC, ANC, and NLR were significantly increased in patients with complex FS compared with simple (p < 0.05). Our findings showed that WBC, MPV, ANC, and NLR were higher in children with FS than in the control group. Additionally WBC, ANC, and NLR were found to be higher in children with CFS than in those with simple febrile seizure. Based on the study results, we suggest that only WBC may be used as predictors in children with FS.

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Platelet Count and Mean Platelet Volume in Hypertensive Patients: A Cross Sectional Study

Indian Journal of Pathology Research and Practice ◽

10.21088/ijprp.2278.148x.6317.31 ◽

2017 ◽

Vol 6 (3 (part-2)) ◽

pp. 693--695

Author(s):

Harish S. Permi ◽

◽

Kishan Prasad H.L. ◽

Sanjana D.S. ◽

Jayaprakash Shetty K ◽

...

Keyword(s):

Platelet Count ◽

Mean Platelet Volume ◽

Cross Sectional Study ◽

Sectional Study ◽

Platelet Volume ◽

Cross Sectional ◽

Hypertensive Patients

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Platelet/lymphocyte ratio and mean platelet volume in patients with granulomatosis with polyangiitis

Advances in Rheumatology ◽

10.1186/s42358-019-0110-8 ◽

2019 ◽

Vol 60 (1) ◽

Author(s):

Hamit Kucuk ◽

Duygu Tecer ◽

Berna Goker ◽

Ozkan Varan ◽

Hakan Babaoglu ◽

...

Keyword(s):

Mean Platelet Volume ◽

Granulomatosis With Polyangiitis ◽

Complete Blood Count ◽

Renal Involvement ◽

Control Group ◽

High Morbidity ◽

Platelet Volume ◽

Cross Sectional ◽

Lymphocyte Ratio ◽

Potential Marker

Abstract Background Granulomatosis with polyangiitis (GPA) is a granulomatous necrotizing vasculitis with high morbidity and mortality. Anti-neutrophil cytoplasmic antibody is a valuable diagnostic marker, however its titer lacks predictive value for the severity of organ involvement. Platelet to lymphocyte ratio (PLR) and mean platelet volume (MPV) has been regarded as a potential marker in assessing systemic inflammation. We aimed to explore the value of PLR and MPV in the assessment of disease activity and manifestations of disease in GPA. Methods 56 newly diagnosed GPA patients and 53 age-sex matched healthy controls were included in this retrospective and cross-sectional study with comparative group. Complete blood count was performed with Backman Coulter automatic analyzer, erythrocyte sedimentation rate (ESR) with Westergen method and C-reactive protein (CRP) levels with nephelometry. The PLR was calculated as the ratio of platelet and lymphocyte counts. Result Compared to control group, ESR, CRP and PLR were significantly higher and MPV significantly lower in GPA patients. In patients group, PLR was positively correlated with ESR and CRP (r = 0.39, p = 0.005 and r = 0.51, p < 0.001, respectively). MPV was negatively correlated with ESR and CRP (r = − 0.31, p = 0.028 and r = − 0.34 p = 0.014, respectively). Patients with renal involvement had significantly higher PLR than patients without renal involvement (median:265.98, IQR:208.79 vs median:180.34 IQR:129.37, p = 0.02). PLR was negatively correlated with glomerular filtration rate (r = − 0.27, p = 0.009). A cut-off level of 204 for PLR had 65.6% sensitivity and 62.5 specificity to predict renal involvement. Conclusion PLR exhibit favorable diagnostic performance in predicting renal involvement in patients with GPA.

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Postpartum hemorrhage risk is driven by changes in blood composition through pregnancy

Scientific Reports ◽

10.1038/s41598-021-98411-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Matthew R. Robinson ◽

Marion Patxot ◽

Miloš Stojanov ◽

Sabine Blum ◽

David Baud

Keyword(s):

Platelet Count ◽

Blood Cell ◽

Postpartum Hemorrhage ◽

Mean Platelet Volume ◽

Blood Count ◽

Complete Blood Count ◽

Platelet Volume ◽

Distribution Width ◽

Cell Counts ◽

Blood Cell Counts

AbstractThe extent to which women differ in the course of blood cell counts throughout pregnancy, and the importance of these changes to pregnancy outcomes has not been well defined. Here, we develop a series of statistical analyses of repeated measures data to reveal the degree to which women differ in the course of pregnancy, predict the changes that occur, and determine the importance of these changes for post-partum hemorrhage (PPH) which is one of the leading causes of maternal mortality. We present a prospective cohort of 4082 births recorded at the University Hospital, Lausanne, Switzerland between 2009 and 2014 where full labour records could be obtained, along with complete blood count data taken at hospital admission. We find significant differences, at a $$p<0.001$$ p < 0.001 level, among women in how blood count values change through pregnancy for mean corpuscular hemoglobin, mean corpuscular volume, mean platelet volume, platelet count and red cell distribution width. We find evidence that almost all complete blood count values show trimester-specific associations with PPH. For example, high platelet count (OR 1.20, 95% CI 1.01–1.53), high mean platelet volume (OR 1.58, 95% CI 1.04–2.08), and high erythrocyte levels (OR 1.36, 95% CI 1.01–1.57) in trimester 1 increased PPH, but high values in trimester 3 decreased PPH risk (OR 0.85, 0.79, 0.67 respectively). We show that differences among women in the course of blood cell counts throughout pregnancy have an important role in shaping pregnancy outcome and tracking blood count value changes through pregnancy improves identification of women at increased risk of postpartum hemorrhage. This study provides greater understanding of the complex changes in blood count values that occur through pregnancy and provides indicators to guide the stratification of patients into risk groups.

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Mean Platelet Volume and Neutrophil/Lymphocyte Ratio among Patients with Ulcerative Colitis as an Indicator of Activation

Lokman Hekim Health Sciences ◽

10.14744/lhhs.2021.70006 ◽

2021 ◽

Vol 1 (1) ◽

pp. 1-4

Author(s):

Kadir Serkan Yalcin

Keyword(s):

Ulcerative Colitis ◽

Platelet Count ◽

Mean Platelet Volume ◽

Complete Blood Count ◽

Activity Index ◽

Platelet Volume ◽

Neutrophil Lymphocyte Ratio ◽

Lymphocyte Ratio ◽

Wbc Count ◽

Two Parameters

Introduction: To determine the changes in mean platelet volume (MPV) and the neutrophil/lymphocyte ratio (NLR) in activation period among patients with ulcerative colitis (UC). Methods: Overall, 83 patients (49 female and 34 male) diagnosed with UC were retrospectively screened and includ- ed in this study. The complete blood count results, including hemoglobin, white blood cell (WBC) count, NLR, platelet count, and MPV, of all patients were recorded in both activation and remission periods. The serum C-reactive protein (CRP) and sedimentation levels of all patients were also recorded. Endoscopic disease activities (endoscopic activity index (EAI)) were also noted. Results: Compared with the remission periods, MPV values were statistically significantly lower (p=0.023) and NLR values were statistically significantly higher (p=0.011) in activation periods. In the correlation analysis, MPV levels were correlated with disease age (p=0.019), hemoglobin (p=0.012), WBC count (p=0.009), platelet count (p=0.001), sedimentation rate (p=0.001), CRP levels (p=0.009), and EAI (p=0.008) during activation periods. Conversely, the NLR did not correlate with MPV, hemoglobin, WBC, platelet count, or sedimentation and CRP levels; however, the NLR was also correlated with EAI (p=0.036). Discussion and Conclusion: We have studied two inexpensive, easily applicable, and noninvasive serum biomarkers, MPV and NLR, to determine UC activation and found that these two parameters are well correlated with other inflam- matory markers and EAI.

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Platelets and D-dimer as the Biomarkers for COVID-19

Pakistan BioMedical Journal ◽

10.54393/pbmj.v4i2.92 ◽

2021 ◽

Vol 4 (2) ◽

Author(s):

Sabrina Rashid ◽

Aqsa Zulfiqar ◽

Ayesha Abdul Sattar ◽

Muhammad Rizwan ◽

Waqar Ali ◽

...

Keyword(s):

Platelet Count ◽

Complete Blood Count ◽

Severe Pneumonia ◽

World Health ◽

Medical Laboratory ◽

P Value ◽

Cross Sectional ◽

Wide Range ◽

Laboratory Technology ◽

D Dimer

COVID-19, a highly contagious and rapidly spreading viral infection caused by the new corona virus SARS-COV-2, was first identified in China. On March11th, 2020 World Health Organization announced it a pandemic. The novel infection which is still being studied has a wide range of severity, ranging from no symptoms to severe pneumonia that can lead to death Objective: To find correlation between D-Dimer and platelets count with the severity of the disease Methods: It was a cross sectional study. The duration of the study was 6 months from November, 2020 to May 2021. The study was conducted in University Institute of Medical Laboratory Technology (UIMLT), University of Lahore. Data of 101 COVID-19 positive patients was taken from Shaukat Khanum Hospital, Farooq Hospital & University of Lahore Teaching Hospital (UOL), Lahore. Hematological and biochemical parameters such as Complete blood count (CBC), D-Dimer, Lactate Dehydrogenase (LDH), Platelet count (PLT) and Ferritin levels were observed. The data was analyzed using Statistical Package for Social Sciences (SPSS) Version 21.0. P-value less than and equal to 0.05 was taken as significant Results: Platelet count was decreased in severe patients of COVID-19 and was statisticallysignificant (P-value = 0.004) LDH, D-dimer, and Ferritin were increased with severity of disease and they were also statistically significant (P-value 0.005, 0.000. 0.027) respectively Conclusions:The platelet count, D-Dimer, LDH and Ferritin levels could act as the biomarker to determine the severity in COVID-19 infection

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