scholarly journals Clinical Application of Platelet-Rich Fibrin in Pediatric Dentistry

2021 ◽  
Author(s):  
Sowndarya Gunasekaran ◽  
Soundarya Sakthivel ◽  
Shanthala B. M. ◽  
George Babu ◽  
Vidhya Vijayan
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Clinical Application ◽  
Pediatric Dentistry ◽  
Simple Process ◽  
Platelet Rich Fibrin ◽  
Promote Wound Healing ◽  
Fibrin Matrix ◽  
Tissue Healing ◽  
Insight Into

AbstractPlatelet-rich fibrin (PRF) is a fibrin matrix in which platelet cytokines, growth factors, and cells are trapped and may be released after a certain time and that can serve as a resorbable membrane. It can be obtained from blood with the help of a simple process. PRF is basically a concentrate of growth factors that promote wound healing and regeneration, which is used in various disciplines of dentistry to repair various lesions and regenerate dental and oral tissues. Although the use of PRF is well-documented, its use in pediatric dentistry remains unexplored, due to its restrictions and on account of the fact that it is a blood-derived product. PRF has been used globally to enhance tissue healing. This article provides an insight into the use of PRF in pediatric dentistry, its benefits, limitations, and recommendations.

scholarly journals Platelet-Rich Fibrin as a Biofuel for Tissue Regeneration

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Sujeet Vinayak Khiste ◽  
Ritam Naik Tari
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Clinical Application ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Vital Role ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Fibrin Network ◽  
New Material

Wound healing is a staged process which involves the activity of leukocytes and platelets. For this process to work efficiently, the platelets play a vital role. The growth factors present in platelets are important to guide the regenerating cells to the area of healing. Platelet-rich-fibrin (PRF) is one such material that holds on to these growth factors enmeshed in the fibrin network resulting in their sustained release over a period of time that can accelerate the wound healing process. With this knowledge, research has been carried out for a past few years for the clinical application of PRF. Various platelet concentrates have been studied including the platelet-rich-plasma (PRP). However, the short duration of cytokine release and its poor mechanical properties have resulted in the search of a new material with adequate properties for clinical application and ease of preparation. PRF has found a place in the regenerative field owing to its advantages over PRP. This review focuses on the properties and various applications of PRF in the clinical practice.

scholarly journals Concentrated Growth Factors vs. Leukocyte-and-Platelet-Rich Fibrin for Enhancing Postextraction Socket Healing. A Longitudinal Comparative Study

2020 ◽  
Vol 10 (22) ◽  
pp. 8256
Author(s):  
Marco Mozzati ◽  
Giorgia Gallesio ◽  
Margherita Tumedei ◽  
Massimo Del Fabbro
Keyword(s):  
Growth Factors ◽  
Oral Surgery ◽  
Platelet Rich Fibrin ◽  
Tissue Healing ◽  
Significant Difference ◽  
Postoperative Symptoms ◽  
Concentrated Growth Factors ◽  
Socket Healing ◽  
And Control ◽  
Tooth Extractions

Platelet concentrates (PCs) have been used for over 20 years in dentistry, as an adjunct to oral surgery procedures, to improve hard and soft tissue healing and control postoperative symptoms. Among various PCs, Leukocyte and Platelet-Rich Fibrin (L-PRF) has become very popular due to its excellent cost-effectiveness ratio, and to the simple preparation protocol, but comparative clinical studies with other PCs are lacking. The aim of this split-mouth cohort study was to evaluate the effect of Concentrated Growth Factors (CGF), a recently introduced PC, as compared to L-PRF for enhancing post-extraction socket healing. Methods: Patients in need of bilateral tooth extractions were included. Each side was treated with either CGF or L-PRF. Pain, socket closure and healing index were the main outcomes. Results: Forty-five patients (24 women), aged 60.52 ± 11.75 years (range 37–87 years) were treated. No significant difference in outcomes was found, except for Pain at day 1 (p < 0.001) and socket closure in the vestibulo-palatal/lingual dimension at day 7 post-extraction (p = 0.04), both in favor of CGF. Conclusions: based on the present results, CGF proved to be as effective and safe as L-PRF, representing a valid alternative option for improving alveolar socket healing and reducing postoperative discomfort.

Extraction Socket Management Utilizing Platelet Rich Fibrin: A Proof-of-Principle Study of the “Accelerated-Early Implant Placement” Concept

2016 ◽  
Vol 42 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Georgios A. Kotsakis ◽  
Foteini Boufidou ◽  
James E. Hinrichs ◽  
Hari S. Prasad ◽  
Michael Rohrer ◽  
...  
Keyword(s):  
Growth Factors ◽  
Treatment Time ◽  
Primary Stability ◽  
Clinical Results ◽  
Recipient Site ◽  
Platelet Rich Fibrin ◽  
Osteoblastic Activity ◽  
Implant Placement ◽  
Tissue Healing ◽  
Proof Of Principle

Dental implants are widely accepted as the golden standard for the rehabilitation of an edentulous site following the extraction of a tooth. The ideal time for implant placement is dependent on the time required for partial or complete tissue healing and the adequacy of socket dimensions. The use of autologous growth factors is a promising new concept that aids clinicians in minimizing treatment time and increasing patient satisfaction. The purpose of this paper is to introduce a protocol for “accelerated-early” implant placement. In this protocol, platelet rich fibrin is employed to accelerate soft and hard tissue healing and to provide a better-healed recipient site for accelerated, early implant placement. Histological analysis revealed that at 6 weeks postextraction, the application of our approach resulted in delicate newly formed bone showing intense osteoblastic activity surrounded by connective tissue as well as areas of mineralized tissue. The present study is a proof-of-principle study of the acceleration of the physiologic postextraction healing sequelae with the use of autologous growth factors. The accelerated-early implant placement concept is a bioengineered protocol that may aid clinicians to achieve increased primary stability, by placing implants in ridges in an advanced stage of bone healing, while offering patients the benefits associated with early implant placement. Controlled studies are warranted to verify the reproducibility of this treatment concept and identify specific indications where the use of the presented technique can lead to significant clinical results.

scholarly journals Modified Preparation Method of Ideal Platelet-Rich Fibrin Matrix (PRFM) from Whole Blood.

2021 ◽  
Author(s):  
Mirta Hediyati Reksodiputro ◽  
Alida Roswita Harahap ◽  
Lyana Setiawan ◽  
Mikhael Yosia
Keyword(s):  
Wound Healing ◽  
Whole Blood ◽  
Preparation Method ◽  
Healing Process ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Platelet Counts ◽  
Fibrin Matrix ◽  
Modified Method ◽  
Commercial Kits

One bioproduct that is widely used in the wound healing process is Platelet Rich Plasma (PRP). PRP is a liquid solution with high autologous platelet concentration, making it a good source of growth factors to accelerate wound healing. Recent development in PRP had created a new product called Platelet Rich Fibrin Matrix (PRFM), which has a denser and more flexible structure. PRFM is the newest generation of platelet concentrate with a fibrin matrix that holds platelet in it. The key concept in creating PRFM from PRP is the addition of CaCl2 followed by centrifugation, which converts fibrinogen to fibrin, and the fibrin cross-links to form a matrix that contains viable platelets. There are many commercially available kits to create PRFM, but they are often expensive and uneconomical. This research will test a modified method of making ideal PRFM from PRP without any commercial kits. The modified method will include determining the minimum level of CaCl2 used, the type of centrifuge, and the speed and duration of centrifugation. By performing a modified preparation method on five samples of whole blood, it was found that the ideal PRFM could be made by mixing PRP with 25 mM CaCl2 1M and centrifuging it at a speed of 2264 G for 25 minutes at room temperature. The PRP and PRFM platelet counts of this method tend to be lower than the platelet counts found in other studies. Although visually comparable, further study is needed to compare the performance of PRFMs made with this method and PRFMs made with commercial kits.

scholarly journals The Clinical Application Of Platelet Concentrates: A Systematic Meta-analysis

2015 ◽  
Vol 5 (1) ◽  
pp. 21-26
Author(s):  
Sneha Ketan Gada
Keyword(s):  
Clinical Application ◽  
Oral Surgery ◽  
Therapeutic Potential ◽  
Platelet Rich Plasma ◽  
Meta Analysis ◽  
Pediatric Dentistry ◽  
Platelet Concentrates ◽  
Bone Contact ◽  
Platelet Rich Fibrin ◽  
Regenerative Dentistry

ABSTRACT Background Autologous plasma rich in platelets is derived blood product whose uses in dentistry dates back to the 1990s and it has been gaining popularity ever since. Aim A systematic review of the available literature to determine the efficacy, safety and success of platelet concentrates in physiological systems as well as describing its vigilant use. Study design and methods A Medline search with keywords ‘platelet rich plasma dental’ further filtered using Boolean opera- tors (AND, OR, NOT) and combination of specific keywords as follows: ‘platelet rich fibrin’, ‘platelet rich plasma regenerative’, ‘platelet rich plasma periodontal’, ‘platelet rich plasma extraction’ with a custom range of 10 years was performed, which yielded 88 results out of which 32 were selected based on the inclusion criteria. Results Significantly, successful outcomes have been wit- nessed in the field of osteoregeneration though it has been evidenced that adequate platelet rich plasma (PrP) additives are essential to bear a therapeutic potential. Favoring blood cell adhesion on the root surface optimizing periodontal healing, PrP has also shown positive effect on gingival repair; though controversial facts have also been reported. In the scope of implant dentistry, PrP has also reported greater implant-bone contact when used in both gel or liquid form. Emerging as a possible pulp capping agent along with properties of decreased chances root growth, PrP has successfully completed apexo- genesis in cases of pulpotomy.   Research has shown that although clinical results from the comparison of PrP alone vs PrP and resorbable membrane or various graft materials has shown a varied results, additional research in the field is needed. Conclusion Platelet rich plasma has shown promising results in the field of regenerative dentistry, with high success rate in implantology, periodontology and oral surgery. Because of conflicting success rates reported in literature further research is warrented. Clinical implication Platelet rich fibrin has displayed various features of rapid clinical healing, excellent bone density, less surgical time, less resorption during healing and decreased postoperative pain, as compared to guided bone regeneration procedures. Platelet rich plasma has also shown a myriad of applications ranging from hard tissue regeneration to soft tissue management, stretching across to the field of pediatric dentistry and endodontics. How to cite this article Gada SK, Gupta P. the Clinical Application of Platelet Concentrates: A Systematic Meta- analysis. Int J Prosthodont restor Dent 2015;5(1):21-26.

scholarly journals Efficacy of platelet-rich fibrin in promoting the healing of extraction sockets: a systematic review

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Sarah Al-Maawi ◽  
Kathrin Becker ◽  
Frank Schwarz ◽  
Robert Sader ◽  
Shahram Ghanaati
Keyword(s):  
Wound Healing ◽  
Soft Tissue ◽  
Tooth Extraction ◽  
Meta Analysis ◽  
Platelet Rich Fibrin ◽  
Hand Search ◽  
Healing Period ◽  
Thomson Reuters ◽  
Tissue Healing ◽  
Pubmed Database

Abstract Purpose To address the focused question: in patients with freshly extracted teeth, what is the efficacy of platelet-rich fibrin (PRF) in the prevention of pain and the regeneration of soft tissue and bone compared to the respective control without PRF treatment? Methods After an electronic data search in PubMed database, the Web of Knowledge of Thomson Reuters and hand search in the relevant journals, a total of 20 randomized and/or controlled studies were included. Results 66.6% of the studies showed that PRF significantly reduced the postoperative pain, especially in the first 1–3 days after tooth extraction. Soft tissue healing was significantly improved in the group of PRF compared to the spontaneous wound healing after 1 week (75% of the evaluated studies). Dimensional bone loss was significantly lower in the PRF group compared to the spontaneous wound healing after 8–15 weeks but not after 6 months. Socket fill was in 85% of the studies significantly higher in the PRF group compared to the spontaneous wound healing. Conclusions Based on the analyzed studies, PRF is most effective in the early healing period of 2–3 months after tooth extraction. A longer healing period may not provide any benefits. The currently available data do not allow any statement regarding the long-term implant success in sockets treated with PRF or its combination with biomaterials. Due to the heterogeneity of the evaluated data no meta-analysis was performed.

scholarly journals A Modified Preparation Method of Ideal Platelet-Rich Fibrin Matrix From Whole Blood

2021 ◽  
Vol 8 ◽  
Author(s):  
Mirta Hediyati Reksodiputro ◽  
Alida Roswita Harahap ◽  
Lyana Setiawan ◽  
Mikhael Yosia
Keyword(s):  
Wound Healing ◽  
Whole Blood ◽  
Preparation Method ◽  
Healing Process ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Platelet Counts ◽  
Fibrin Matrix ◽  
Modified Method ◽  
Commercial Kits

One bioproduct that is widely used in the wound healing process is platelet-rich plasma (PRP). PRP is a liquid solution with high autologous platelet concentration, making it a good source of growth factors to accelerate wound healing. Recent development in PRP had created a new product called platelet-rich fibrin matrix (PRFM), which has a denser and more flexible structure. PRFM is the newest generation of platelet concentrate with a fibrin matrix that holds platelet in it. The key concept in creating PRFM from PRP is the addition of CaCl2 followed by centrifugation, which converts fibrinogen to fibrin, and the fibrin cross-links to form a matrix that contains viable platelets. There are many commercially available kits to create PRFM, but they are often expensive and uneconomical. This research will test a modified method of making ideal PRFM from PRP without any commercial kits. The modified method will include determining the minimum level of CaCl2 used, the type of centrifuge, and the speed and duration of centrifugation. By performing a modified preparation method on five samples of whole blood, it was found that the ideal PRFM could be made by mixing PRP with 25 mM CaCl2 and centrifuging it at a speed of 2,264 × g for 25 min at room temperature. The PRP and PRFM platelet counts of this method tend to be lower than the platelet counts found in other studies. Although visually comparable, further study is needed to compare the performance of PRFMs made with this method and PRFMs made with commercial kits.

The dual delivery of growth factors and antimicrobial peptide by PLGA/GO composite biofilms to promote skin-wound healing

2020 ◽  
Vol 44 (4) ◽  
pp. 1463-1476
Author(s):  
Ziyan Zhang ◽  
Shicheng Zhou ◽  
Yanzhe Zhang ◽  
Dankai Wu ◽  
Xiaoyu Yang
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Antimicrobial Peptide ◽  
Medical Devices ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Promote Wound Healing ◽  
Biodegradable Biomaterials

Biodegradable biomaterials coated with active factors are effective medical devices to promote wound healing.

scholarly journals Cloning, Expression and Effects of P. americana Thymosin on Wound Healing

2019 ◽  
Vol 20 (19) ◽  
pp. 4932 ◽  
Author(s):  
Jie Jing ◽  
Xiaohong Sun ◽  
Chuang Zhou ◽  
Yifan Zhang ◽  
Yongmei Shen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Tissue Regeneration ◽  
Dna Sequences ◽  
Wound Repair ◽  
Periplaneta Americana ◽  
American Cockroach ◽  
Promote Wound Healing ◽  
Fibroblast Migration

The American cockroach (Periplaneta americana) is a medicinal insect. Its extract is used clinically to promote wound healing and tissue regeneration, but the effective medicinal components and mechanisms are not yet clear. It has been reported that human thymosin beta 4 (Tβ4) may accelerate skin wound healing, however, the role of P. americana thymosin (Pa-THYs) is still poorly understood. In the present study, we identify and analyze the DNA sequences of Pa-THYs by bioinformatics analysis. Then we clone, express, and purify the Pa-THYs proteins and evaluate the activity of recombinant Pa-THYs proteins by cell migration and proliferation assays in NIH/3T3 cells. To elucidate the role of Pa-THYs in wound healing, a mouse model is established, and we evaluate wound contraction, histopathological parameters, and the expressions of several key growth factors after Pa-THYs treatment. Our results showed that three THY variants were formed by skipping splicing of exons. Pa-THYs could promote fibroblast migration, but have no effect on fibroblast proliferation. In wound repair, Pa-THYs proteins could effectively promote wound healing through stimulating dermal tissue regeneration, angiogenesis, and collagen deposition. On the molecular mechanism, Pa-THYs also stimulated the expression of several key growth factors to promote wound healing. The data suggest that Pa-THYs could be a potential drug for promoting wound repair.

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