Comparison of bacterial populations in bedding material, on teat ends, and in milk of cows housed in compost bedded pack barns

2018 ◽  
Vol 58 (9) ◽  
pp. 1686 ◽  
Author(s):  
R. L. Albino ◽  
J. L. Taraba ◽  
M. I. Marcondes ◽  
E. A. Eckelkamp ◽  
J. M. Bewley
Keyword(s):  
Escherichia Coli ◽  
Somatic Cell ◽  
Somatic Cell Count ◽  
Bacterial Population ◽  
Bedding Material ◽  
Efficient Tool ◽  
Bacterial Populations ◽  
Positive Correlation ◽  
Lactating Cows ◽  
Total Bacteria

The objective of this study was to observe relationships among somatic cell count (SCC) and bacteria counts in milk, on teat ends of lactating cows, and in compost samples from the aerated layer of the compost bedded pack. Twenty-nine lactating cows were used in this study. Clinically mastitis cows were not selected for the trial. The correlation between total bacteria count (TBC) and Streptococcus spp. of teat end and hygiene score was (r = 0.49) and (0.44, P = 0.01), respectively. In addition, there was a positive correlation (0.40, P = 0.03) between TBC on teat ends and somatic cell score. When analysing bacterial populations on teat ends and in milk, there was a positive correlation (0.39, P = 0.03) between Escherichia coli at the teat end and coliform counts in milk and also a positive correlation (0.38, P = 0.04) between coliform counts at the teat end and milk. Furthermore, Streptococcus spp. counts on teat end were positively correlated (0.38, P = 0.04) with TBC in milk. Although correlations were observed between hygiene score and SCC with bacterial population, all correlations were moderate. Therefore, hygiene score was not an efficient tool to estimate bacterial populations on teat end and milk.

COUNTING TOTAL BACTERIA IN LAND ORGANIC WASTE HOUSEHOLD AND LAND INORGANIC WITH TOTAL PLATE COUNT METHOD (TPC)

2017 ◽  
Vol 4 (2) ◽  
pp. 87-91
Author(s):  
Ekamaida Ekamaida
Keyword(s):  
Soil Fertility ◽  
Bacterial Population ◽  
Organic Soil ◽  
Biological Properties ◽  
Plate Count ◽  
Bacterial Populations ◽  
Fertility Data ◽  
Plate Count Method ◽  
The Mean ◽  
Total Bacteria

The soil fertility aspect is characterized by the good biological properties of the soil. One important element of the soil biological properties is the bacterial population present in it. This research was conducted in the laboratory of Microbiology University of Malikussaleh in the May until June 2016. This study aims to determine the number of bacterial populations in soil organic and inorganic so that can be used as an indicator to know the level of soil fertility. Data analysis was done by T-Test that is by comparing the mean of observation parameter to each soil sample. The sampling method used is a composite method, which combines 9 of soil samples taken from 9 sample points on the same plot diagonally both on organic soil and inorganic soil. The results showed the highest bacterial population was found in total organic soil cfu 180500000 and total inorganic soil cfu 62.500.000

scholarly journals Differential Somatic Cell Count as a Marker for Changes of Milk Composition in Cows with Very Low Somatic Cell Count

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 604 ◽  
Author(s):  
Alfonso Zecconi ◽  
Francesca Dell’Orco ◽  
Diego Vairani ◽  
Nicoletta Rizzi ◽  
Micaela Cipolla ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Milk Fat ◽  
Somatic Cell Count ◽  
Milk Composition ◽  
Cow Milk ◽  
Udder Health ◽  
Lactating Cows ◽  
Accuracy Of Measurements ◽  
The Relationship

The recent availability of a high-throughput milk analyzer performing a partial differential somatic cell count (DSCC) opened new opportunities in investigations on bovine udder health. This analyzer has a potential limitation on the accuracy of measurements when the somatic cell count (SCC) is below 50,000 cells/mL, values characterizing a good proportion of lactating cows in many herds. We obtained data for cows below this threshold, assessed the repeatability of these measurements and investigated the relationship between DSCC and udder health, milk composition and yield. Overall, 3022 cow milk test records performed on a Fossomatic™ 7/DC (Foss A/S, Hillerød, Denmark) were considered; 901 of them had an SCC ≤ 50,000 cells/mL. These latter samples were analyzed by qPCR to identify the presence of bacteria. Overall, 20.75% of the samples (187) were positive. However, the health status did not have any significant association with DSCC. The analysis of the association of DSCC on milk fat, protein and casein showed a significant decrease in their proportions as the DSCC increased, whereas it was not observed for milk yield and lactose. Therefore, DSCC in very low SCC cows may be suggested as a marker to identify early changes in milk composition.

scholarly journals An effect of mammary gland infection caused by Streptococcus uberis on composition and physicochemical changes of cows’ milk

2016 ◽  
Vol 19 (1) ◽  
pp. 49-55 ◽  
Author(s):  
E. Pecka-Kiełb ◽  
M. Vasil ◽  
A. Zachwieja ◽  
W. Zawadzki ◽  
J. Elečko ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Physicochemical Properties ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Milk Composition ◽  
Microbiological Analysis ◽  
Milk Samples ◽  
Streptococcus Uberis ◽  
Total Bacteria

Abstract An effect of mammary gland infection caused by Streptococcus uberis on the changes in cows’ milk composition and its physicochemical properties was examined. The study was conducted in the herd of Slovak Pied breed cattle (with a share of HF blood), in 2nd and 3rd lactation, after 4th month of milking. Milk samples were collected from a quarter milking. The samples were subjected to microbiological analysis, basic milk composition, total bacteria count, somatic cell count and physicochemical properties were examined. Also analyses of protein fractions share and fatty acids profile were conducted. An effect of bacterial infection of the mammary gland bring an increase (P<0.01) in somatic cell count was observed in this study. Milk samples contaminated with S. uberis were characterized by higher (P<0.05) total bacteria count and total protein compared to milk samples collected from non-infected mammary gland. The level of κ-casein was significantly (P<0.05) decreased in cows with subclinical mastitis caused by S. uberis. Significant (P<0.05) reduction in the share of C13:0 acid, and an increased level of C18:0, C18:1n7t and CLA were observed in milk contaminated with S. uberis compared to healthy cows’ milk. It should be concluded that S. uberis causes the increase in total bacteria count, SCC and the decrease in κ-casein level, which significantly affects deterioration of technological quality of cows’ milk.

scholarly journals Association among Feeding After Milking, Animal Factors and Post-milking Standing Period and Effect of Post-milking Standing Period on Intra- Mammary Infection in Crossbred Cows

2019 ◽  
Author(s):  
Champak Bhakat
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Monthly Interval ◽  
Feeding Time ◽  
Microscopic Method ◽  
Crossbred Cows ◽  
Lactating Cows ◽  
The Mean ◽  
Time Of Feeding

The present investigation was undertaken to study the association of time of feeding after milking andanimal factors with postmilkingstanding period (PMSP) and effect of PMSP on the milk somatic cell count(SCC)) in crossbred cows. 24 lactating cows were selected and divided into 2 groups, each of 12 (G1:feeding within 15 min after milking and G2: feeding after 90 min of milking). PMSP was recorded forindividual animal and categorized into &lt; 90 min and &gt; 90 min and milk sampling was done and subjectedto microscopic method of somatic cell count. All procedure was performed at monthly interval for 4 monthof duration. The estimated overall mean (±SE) of SCC (logarithmic) was 5.377 ± 0.039. Time of feedingafter milking was significantly (P&lt;0.05) associated with PMSP as the higher percentage (72.92%) ofanimals were found under the PMSP of &gt; 90 minutes in group G1 as compared to G2. The PMSP wassignificantly (P&lt;0.01) affected the SCC level and the mean SCC(±SE) was 5.965±0.056 in the animals thatlie down before 90 minutes after milking while in the animals that lie down after 90 min, mean SCC(±SE)was 5.037±0.037. The association of parity and production level with the PMSP was also significant(P&lt;0.01) and primiparous and medium producer cows showed longer duration of PMSP. Observationsrevealed that PMSP can be managed by managing feeding time and can be utilized for maintaining betterudder health.

Does physical state of farm housing and milking practices affect total bacteria and somatic cell count of cow milk?

2019 ◽  
Vol 39 (3) ◽  
pp. e12635
Author(s):  
Annah Shingirai Paraffin ◽  
Titus Jairus Zindove ◽  
Michael Chimonyo
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Physical State ◽  
Cow Milk ◽  
Total Bacteria

scholarly journals Impact of robot scrapers on clinical mastitis and somatic cell count in lactating cows

2017 ◽  
Vol 46 (1) ◽  
pp. 467-470 ◽  
Author(s):  
Renate Luise Doerfler ◽  
Wolfram Petzl ◽  
Anna Rieger ◽  
Heinz Bernhardt
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Clinical Mastitis ◽  
Lactating Cows

Observations onCorynebacterium bovisinfection of the bovine mammary gland. II. Experimental infection

1984 ◽  
Vol 51 (3) ◽  
pp. 379-385 ◽  
Author(s):  
Tuula Honkanen-Buzalski ◽  
A. John Bramley
Keyword(s):  
Mammary Gland ◽  
Somatic Cell ◽  
Cell Count ◽  
Experimental Infection ◽  
Somatic Cell Count ◽  
Cellular Response ◽  
Clinical Mastitis ◽  
Bovine Mammary Gland ◽  
Lactating Cows ◽  
Do So

SummaryExperiments are described in which lactating cows were exposed toCorynebacterium boviseither by dipping the teats in a suspension of the bacteria or by inoculating the bacteria into the teat duct or the teat sinus. All three methods readily led to ‘infection’ being established. The effect of these infections on somatic cell count was minor and no clinical mastitis resulted during the course of the experiments although some cases occurred subsequently. There was evidence that 44% of these infections were confined to the teat duct. Quarters excretingC. bovisin milk continued to do so during endotoxin-induced inflammation and showed a similar cellular response to that of uninfected quarters.

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