Contributions of glutenin and puroindoline genes to grain quality traits in southern Australian wheat breeding programs

2006 ◽  
Vol 57 (2) ◽  
pp. 179 ◽  
Author(s):  
H. A. Eagles ◽  
Karen Cane ◽  
R. F. Eastwood ◽  
G. J. Hollamby ◽  
Haydn Kuchel ◽  
...  
Keyword(s):  
Water Absorption ◽  
Development Time ◽  
Grain Quality ◽  
Genotypic Variation ◽  
Wheat Breeding ◽  
Wagga Wagga ◽  
Breeding Programs ◽  
Dough Development Time ◽  
Milling Yield

Glutenin genes were known to influence maximum dough resistance (Rmax), dough extensibility (extensibility), and dough development time, whereas puroindoline genes were known to influence grain hardness, flour water absorption (water absorption), and milling yield. These are important determinants of grain quality of wheat in Australia. This study was conducted to investigate the combined effect of these genes on Rmax, extensibility, dough development time, water absorption, and milling yield in a large dataset assembled from the breeding programs based at Horsham, Victoria; Roseworthy, South Australia; and Wagga Wagga, New South Wales; for at least 10 seasons. The effect of the glutenin genes on Rmax, extensibility, and dough development time was confirmed, as was the effect of the puroindoline genes on water absorption and milling yield. In addition, puroindoline genes were shown to significantly affect extensibility and dough development time. The Pina-D1a/Pinb-D1b genotype increased extensibility, dough development time, and milling yield relative to the Pina-D1b/Pinb-D1a genotype. Both of these genotypes are present in cultivars classified as hard-grained in southern Australia. Therefore, the allelic composition of both glutenin and puroindoline genes is required to predict the grain quality of hard wheat in southern Australian breeding programs. The glutenin and puroindoline genes in combination accounted for more than 50% of the genotypic variance for these traits, except for milling yield, but a substantial proportion of the genotypic variation could not be attributed to these genes, indicating that other genes affecting the traits were present in the populations of these wheat-breeding programs.

Genetic and environmental variation for grain quality traits routinely evaluated in southern Australian wheat breeding programs

2002 ◽  
Vol 53 (9) ◽  
pp. 1047 ◽  
Author(s):  
H. A. Eagles ◽  
G. J. Hollamby ◽  
R. F. Eastwood
Keyword(s):  
Water Absorption ◽  
Development Time ◽  
Grain Quality ◽  
Wheat Breeding ◽  
Breeding Programs ◽  
Environmental Correlations ◽  
Flour Protein ◽  
Australian Wheat ◽  
Dough Development Time ◽  
Milling Yield

Milling yield, maximum dough resistance (Rmax), dough extensibility, flour protein concentration (flour protein), particle size index (PSI), water absorption, and dough development time are important determinants of grain quality and are routinely evaluated in Australian wheat breeding programs. Information on allelic variation at the 6 loci determining glutenin proteins is also regularly obtained and used to predict Rmax and extensibility. For each character, except dough development time, 4029 observations on 2377 lines and 94 environments were analysed to estimate genotypic and environmental variances, heritabilities, genotypic and environmental correlations, and the effects of glutenin genes. A subset was analysed for dough development time. Milling yield, Rmax, extensibility, PSI, water absorption, and dough development time had intra-class correlation coefficients, or broad-sense heritabilities, between 0.66 and 0.76, and extensibility had a value of 0.52, with flour protein at 0.36. Genotypic and environmental correlations between extensibility and flour protein were high at +0.78 and +0.85, respectively. Rmax had a genotypic correlation with dough development time of +0.67, which was substantially due to pleiotropic effects of glutenin genes. Rmax, extensibility, PSI, and dough development time were influenced by glutenin genes. For Rmax about 50% of the genotypic variance could be explained by glutenin genes. For extensibility about 50% could be explained by flour protein, with 50% of the remainder by the inclusion of glutenin genes. For dough development time about 15% could be explained by flour protein, with a further 30% by glutenin genes. For PSI, about 40% of the genotypic variation could be accounted for by glutenin genes after the removal of the effects of flour protein and milling yield. We concluded that dough development time could be added to Rmax and extensibility as a trait that can be usefully predicted by the glutenin genes, but more work is required for PSI.

Effect of geographic location of growth on wheat milling yield, farinograph properties, flour protein and residue protein

1977 ◽  
Vol 28 (1) ◽  
pp. 5 ◽  
Author(s):  
L O'Brien ◽  
RA Orth
Keyword(s):  
Water Absorption ◽  
Protein Content ◽  
Development Time ◽  
Protein Quality ◽  
Geographic Location ◽  
Wheat Breeding ◽  
Flour Milling ◽  
Flour Protein ◽  
Dough Development Time ◽  
Milling Yield

The relationship between farinograph dough breakdown and the proportion of flour protein insoluble in 0 . 0 5M acetic acid (residue protein) was investigated for a number of wheats each grown at six locations in the Mallee and Wimmera regions of Victoria. At each location a highly significant correlation was obtained (R ranging from 0.84 to 0.93), which indicated that the 'residue test' could be used as a selection tool in wheat-breeding programs. Regressions of flour milling yield, flour protein content, farinograph water absorption, dough development time and dough breakdown, and the proportion of residue protein were calculated for each parameter for the wheats grown at Dooen against those for wheats grown at each other location. Variables largely dependent on protein 'quality', viz. dough breakdown, dough development time and residue protein, ranked the wheats similarly at each location of growth. Rankings according to milling yield, farinograph water absorption and flour protein content differed more markedly between locations.

Puroindoline genes and their effects on grain quality traits in southern Australian wheat cultivars

2004 ◽  
Vol 55 (1) ◽  
pp. 89 ◽  
Author(s):  
Karen Cane ◽  
Merrin Spackman ◽  
H. A. Eagles
Keyword(s):  
Water Absorption ◽  
Grain Quality ◽  
High Water ◽  
Major Effect ◽  
Wheat Breeding ◽  
Quality Data ◽  
Breeding Programs ◽  
Australian Wheat ◽  
End Use ◽  
Milling Yield

Grain hardness is a major determinant of the classification and end-use of wheat. Two genes, Pina-D1 and Pinb-D1, have a major effect on this trait, so for wheat breeding programs it is important to identify the alleles of these genes present in elite germplasm. This study was conducted to identify the alleles present in southern Australian germplasm, and to determine if they affected quality characteristics other than grain hardness.Only 3 genotypes were identified. These were Pina-D1a/Pinb-D1a producing soft grain, Pina-D1a/Pinb-D1b producing moderately hard grain, and Pina-D1b/Pinb-D1a producing very hard grain. WW15 was the probable source of Pina-D1a/Pinb-D1b in most cultivars; however, Halberd represented another source. An important source of Pina-D1b/Pinb-D1a was the CIMMYT line Pavon, with sources from the old Australian cultivars Gabo and Falcon probably still present in modern germplasm.In an analysis of grain quality data from the Victorian Institute for Dryland Agriculture breeding program, the Pina-D1b/Pinb-D1a genotype had a significantly higher water absorption and significantly lower milling yield than the Pina-D1a/Pinb-D1b genotype, which indicates that these genes will impede the development of hard-grained cultivars that combine high water absorption and high milling yield.

Revision of the estimates of glutenin gene effects at the Glu-B1 locus from southern Australian wheat breeding programs

2004 ◽  
Vol 55 (10) ◽  
pp. 1093 ◽  
Author(s):  
H. A. Eagles ◽  
R. F. Eastwood ◽  
G. J. Hollamby ◽  
E. M. Martin ◽  
G. B. Cornish
Keyword(s):  
Development Time ◽  
Low Frequency ◽  
Wheat Breeding ◽  
Correct Identification ◽  
Gene Effects ◽  
Breeding Programs ◽  
Multiple Alleles ◽  
Intermediate Allele ◽  
Dough Extensibility ◽  
Dough Development Time

Glutenins are the major determinant of dough characteristics in wheat. These proteins are determined by genes at 6 loci, with multiple alleles present in southern Australian breeding programs. Previously, we estimated the effects of these genes on maximum dough resistance (Rmax), dough extensibility and dough development time. Subsequently, the allele previously classified as Glu-B1b was found to consist of 2 alleles, with one, now considered to be Glu-B1al, producing an overexpression of the Bx7 glutenin subunit. Therefore, there is a potential bias in our previous estimates. An extended dataset was analysed with the 2 alleles now separated. These analyses identified negligible biases in our previous estimates, probably due to a low frequency of Glu-B1al before 1999. However, Glu-B1al produced significantly higher Rmax, dough extensibility, and dough development time values than all other alleles at the Glu-B1 locus. Therefore, at intermediate allele frequencies, substantial bias in estimates of the effects of the Glu-B1 alleles can be expected without correct identification of Glu-B1al.

scholarly journals Effect of Different Combinations of Gums and Emulsifiers on the Quality of Bread

2016 ◽  
Vol 59 (1) ◽  
pp. 33-37
Author(s):  
Muhammad Rizwan Haider ◽  
Ghulam Mueen ud din ◽  
Muhammad Abrar ◽  
Arshad Mehmood ◽  
Ashiq Hussain ◽  
...  
Keyword(s):  
Water Absorption ◽  
Development Time ◽  
Guar Gum ◽  
Wheat Variety ◽  
Grain Texture ◽  
Rheological Study ◽  
Stability Time ◽  
Dough Development Time ◽  
Bread Wheat Variety

A project was designed to evaluate the effect of different combinations of emulsifiers and gumson the quality of bread. Wheat variety AARI-11 was milled to get straight grade flour and mixed with theEmulsifiers (DMG & DATEM) and Gums (G.G & CMC) in a quantity of (0.3- 0.6 %). Both, straight gradeflour as well as treated flour (combination with gums and emulsifiers) were subjected to proximate andrheological analysis. Results of the rheological study showed a significant change in water absorption,dough development time, dough stability time and dough viscosity i.e. W/A 61.33-62.93%, D.D.T 3.9-4.8 min, D.S.T 7-9.1 min and 818.33-950.00 BU, respectively. Breads prepared with both flours were alsostudied for their sensory attributes during storage after the interval of 24 h. The highest score was awardedto T1 (0.3% DATEM & 0.5% guar gum) on the bases of its excellent external attributes (colour of crust,volume, symmetry of form, evenness of bake and crust character) and internal characteristics (aroma, grain,texture, taste, mastication and colour of crumb). After the sensory and physicochemical analyses, it isconcluded that with the addition of DATEM (0.3%) and guar gum (0.5%) resulted in good quality of bread.

scholarly journals The effects on grain quality traits of a grain serpin protein and the VPM1 segment in southern Australian wheat breeding

2008 ◽  
Vol 59 (10) ◽  
pp. 883 ◽  
Author(s):  
Karen Cane ◽  
P. J. Sharp ◽  
H. A. Eagles ◽  
R. F. Eastwood ◽  
G. J. Hollamby ◽  
...  
Keyword(s):  
Grain Quality ◽  
Protein A ◽  
Quality Parameters ◽  
Wheat Breeding ◽  
Relationship Matrix ◽  
Quality Traits ◽  
Breeding Programs ◽  
Active Selection ◽  
Australian Wheat ◽  
Milling Yield

Production of wheat of sufficient quality to meet market demands is an ongoing agricultural challenge. Identification and evaluation of alleles of genes affecting quality parameters enables breeders to improve their germplasm by active selection towards specific allele combinations. Using a large dataset obtained from southern Australian wheat breeding programs, and including a relationship matrix in the analysis to minimise bias, we re-evaluated the effects of high- and low-molecular-weight glutenin alleles and puroindoline alleles on the grain quality parameters Rmax, dough extensibility, dough development time, flour water absorption, and milling yield and found that estimated effects were in close agreement with those from earlier analyses without a relationship matrix. We also evaluated, for the first time, the effects on the same quality parameters of 2 alleles (wild-type and null) of a defence grain protein, a serpin located on chromosome 5B. In addition, we assessed the effect of the VPM1 alien segment. The serpin null allele significantly reduced milling yield by ~0.4 g of flour per 100 g of grain milled across different germplasm sources and flour protein levels. In Australian germplasm, the origin of this allele was traced to a 19th Century introduction from India by William Farrer; however other sources, of significance in international breeding programs, were also identified. Our analysis of the effect of the VPM1 segment on quality traits revealed no detrimental effects of its presence on the traits we measured.

CHARACTERISTICS OF MILLING YIELD OF RICE VARIETIES OF RUSSIAN BREEDING GROWN IN VARIOUS CONDITIONS OF NITROGEN NUTRITION IN THE ABINSKIY DISTRICT OF THE KRASNODAR REGION

2020 ◽  
Author(s):  
E.Y. Papulova ◽  
◽  
K.K Olkhovaya
Keyword(s):  
Grain Quality ◽  
Rice Yield ◽  
Nitrogen Nutrition ◽  
Nitrogen Fertilizers ◽  
Rice Varieties ◽  
Head Rice ◽  
Cultivation Technology ◽  
Milling Yield ◽  
Different Doses

The work shows that application of increased doses of nitrogen does not significantly affect the total milling yield and affects the head rice content. The ambiguous nature of the variability of the grain quality of rice varieties under conditions of different doses of nitrogen fertilizers confirms the need for further studies of the varietal reaction to the level of nitrogen nutrition, based on it - the development of rice cultivation technology modes in order to obtain rice yield with high grain quality.

scholarly journals Effects of spray-dried sourdough on flour characteristics and rheological properties of dough

2013 ◽  
Vol 31 (No. 4) ◽  
pp. 361-367 ◽  
Author(s):  
A.G. Tafti ◽  
S.H. Peighardoust ◽  
F. Behnam ◽  
A. Bahrami ◽  
R. Aghagholizadeh ◽  
...  
Keyword(s):  
Water Absorption ◽  
Development Time ◽  
Ph Values ◽  
Powder Addition ◽  
Dough Extensibility ◽  
Dough Development Time ◽  
Wheat Flours ◽  
Different Levels ◽  
Control Water ◽  
Spray Dried

The effect of incorporating different levels of spray-dried sourdough (3, 6, 9, and 15% w/w) on flour characteristics and dough properties of two wheat flours was studied. As the spray-dried sourdough level in the blends increased, the pH values significantly (P < 0.05) decreased. Wet gluten content and sedimentation values were decreased in the flours containing spray-dried sourdough compared to those of the control. Water absorption significantly increased compared to that of the control. However, the dough development time was not affected by sourdough powder addition. Degree of softening significantly increased with an increase in the sourdough level and dough stability was significantly reduced. Doughs incorporating sourdough powder showed higher resistance to extension and lower dough extensibility than the control doughs.  

scholarly journals Morpho-agronomic characterization and estimates of genetic parameters in annatto plant

2017 ◽  
Vol 35 (2) ◽  
pp. 242-246 ◽  
Author(s):  
Nilma O Dias ◽  
Tiyoko NH Rebouças ◽  
Abel R São José ◽  
Cláudio LF Amaral
Keyword(s):  
Genetic Parameters ◽  
Environmental Influence ◽  
Genotypic Variation ◽  
Morphological Characterization ◽  
Breeding Programs ◽  
Tropical America ◽  
Self Fertilization ◽  
Superior Genotypes ◽  
Increasing Demand

ABSTRACT Originally from tropical America, annatto is an important source of natural dyes for food, pharmaceutical and textile industries, especially for the increasing demand of this pigment substituting artificial dyes. The aim of this study was to evaluate genotypic variation of annatto cultivar Embrapa 37, aiming to generate subsidies for breeding programs as well as to help out the producers using vegetative propagation of superior genotypes. Therefore, the morphological characterization was carried out and estimates of genetic and phenotypic parameters, for production and quality of the fruits, were obtained. The results showed wide rate of genetic variability among the plants with low environmental influence on the phenotype for traits bixin content, fruit width, number of fruits per bunch and mass of 100 seeds. The authors concluded that superior genotypes can be selected through the parental performance and propagated vegetatively to form more profitable crops, as well as used in breeding programs through self-fertilization with analysis of resulting progeny.

scholarly journals Development of Highly Nutritional Breads with By-Products of Chia (Salvia hispanica L.) Seeds

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 819 ◽  
Author(s):  
Estefanía Nancy Guiotto ◽  
Mabel Cristina Tomás ◽  
Claudia Mónika Haros
Keyword(s):  
Wheat Flour ◽  
Oil Content ◽  
Development Time ◽  
Colour Parameters ◽  
Bread Crust ◽  
Technological Quality ◽  
Salvia Hispanica L ◽  
Dough Development Time ◽  
By Products

The effect of the incorporation of various types of residual chia flour (whole, semi-defatted and defatted, with or without mucilage) on the technological quality of bread was investigated. The various types of chia flour were used to substitute 5 and 10% wt/wt of wheat flour in the bread formulations. The water absorption, dough development time and stability of blends with the presence of mucilage and the incorporation of 10% wt/wt of chia flour demonstrated the highest values in comparison with the other ones. The specific volume of the flour variants with 5% wt/wt of chia flour with mucilage were similar to the control bread; while those formulated with chia flour without mucilage exhibited a lesser volume. The incorporation of 10% wt/wt of chia flour in the formulations caused a decrease in the technological quality of the bread as expected. The bread crust and crumb colour parameters were mainly influenced by the level of chia flour substitution, which resulted in a decrease in lightness and h values. The substitution of wheat flour with 5% wt/wt of chia flour counterparts with mucilage improved the technological quality of the breads. The different oil content of the chia flours did not show any significant influence on overall quality or texture.

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