Genome-wide identification of AP2/ERF transcription factors in mungbean (Vigna radiata) and expression profiling of the VrDREB subfamily under drought stress

Mungbean (Vigna radiata L.) is a valuable legume crop grown in tropical and subtropical areas of Asia. Drought is one of the major factors hindering its growth globally. APETALA2/ethylene-responsive element factor binding proteins (AP2/ERF) are an important family of plant-specific transcription factors (TFs) involved in drought-stress tolerance. We identified 71 AP2/ERF TFs in the mungbean genome by using bioinformatics tools and classified them into subfamilies: AP2 (16 members), ERF (22), RAV (2), DREB (30) and soloist (other proteins with no domain, 1). Members of DREB play a critical role in drought-stress tolerance. Ten-day-old mungbean plants cv. AZRI-06 were exposed to drought stress by complete withholding of water for 7 days. Root samples were collected from control and drought-stressed plants, and the expression pattern of 30 identified VrDREB genes was determined by qPCR. Most VrDREB genes exhibited differential expression in response to drought. Five genes (VrDREB5, VrDREB12, VrDREB13, VrDREB22, VrDREB30) were highly expressed under drought stress and might be considered excellent candidates for further functional analysis and for improvement of mungbean drought tolerance.

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Genome-wide association study and genomic prediction analyses of drought stress tolerance in China in a collection of off-PVP maize inbred lines

Molecular Breeding ◽

10.1007/s11032-019-1013-4 ◽

2019 ◽

Vol 39 (8) ◽

Cited By ~ 3

Author(s):

Nan Wang ◽

Bojuan Liu ◽

Xiaoling Liang ◽

Yueheng Zhou ◽

Jie Song ◽

...

Keyword(s):

Drought Stress ◽

Association Study ◽

Stress Tolerance ◽

Genomic Prediction ◽

Genome Wide Association Study ◽

Inbred Lines ◽

Genome Wide Association ◽

Drought Stress Tolerance ◽

Maize Inbred Lines ◽

Genome Wide

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Genome-wide identification and expression analysis of SnRK2 gene family in mungbean (Vigna radiata) in response to drought stress

Crop and Pasture Science ◽

10.1071/cp19392 ◽

2020 ◽

Vol 71 (5) ◽

pp. 469

Author(s):

Annaira Fatima ◽

Muhammad Jadoon Khan ◽

Hassaan Mehboob Awan ◽

Malik Nadeem Akhtar ◽

Nazia Bibi ◽

...

Keyword(s):

Drought Stress ◽

Gene Family ◽

Real Time ◽

Vigna Radiata ◽

Crop Production ◽

Gene Prediction ◽

Critical Role ◽

Evolutionary Analysis ◽

Genome Wide Analysis ◽

Genome Wide

Drought stress causes lower crop production globally. Plants have acquired many adaptations to overcome drought stress. Mungbean (Vigna radiata (L.) R.Wilczek) is a legume crop widely cultivated in South, East and Southeast Asia. It is grown in high-temperature areas where drought is the main cause of reduced plant growth and productivity. Plants cope with drought stress by activating different signalling mechanisms. The sucrose non-fermenting-1-related protein kinase 2 family (SnRK2s) is known to play vital roles in osmotic stress and in abscisic acid (ABA) signalling pathways by phosphorylating downstream targets. The genes encoding SnRK2s in mungbean and their detailed characterisation remain unexplored. We have conducted extensive genome-wide analysis for gene prediction, in silico gene analysis, evolutionary analysis and gene-expression profiling under drought-stress conditions by quantitative real-time PCR. Through genome-wide analysis, eight SnRK2 genes were predicted in the mungbean genome and were assigned the names VrSnRK2.1–VrSnRK2.8, according to their order on the chromosomes. The VrSnRK2 genes identified were classified into three clusters based on their phylogenetic relationship with those of Arabidopsis thaliana. Drought stress was imposed on 11-day-old mungbean plants by completely withholding water for 3 days. According to real-time qPCR data, the expression of most of the VrSnRK2 genes was induced by drought stress, indicating their role in the drought-stress response. One of the genes, namely SnRK2.6c, showed highest expression level (12-fold) under drought stress, possibly indicating a critical role under water-deficit conditions. These data provide important information about the VrSnRK2 gene family in mungbean. The results will help in future functional characterisation of VrSnRK2 genes.

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WITHDRAWN: Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed

Oil Crop Science ◽

10.1016/j.ocsci.2021.01.001 ◽

2021 ◽

Author(s):

Ali Shahzad ◽

Minchao Qian ◽

Bangyang Sun ◽

Umer Mahmood ◽

Shengting Li ◽

...

Keyword(s):

Drought Stress ◽

Association Study ◽

Stress Tolerance ◽

Candidate Genes ◽

Genome Wide Association Study ◽

Genome Wide Association ◽

Drought Stress Tolerance ◽

Genome Wide

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Genome-Wide Analysis of MADS-Box Genes in Foxtail Millet (Setaria italica L.) and Functional Assessment of the Role of SiMADS51 in the Drought Stress Response

Frontiers in Plant Science ◽

10.3389/fpls.2021.659474 ◽

2021 ◽

Vol 12 ◽

Author(s):

Wan Zhao ◽

Li-Li Zhang ◽

Zhao-Shi Xu ◽

Liang Fu ◽

Hong-Xi Pang ◽

...

Keyword(s):

Drought Stress ◽

Stress Tolerance ◽

Abiotic Stresses ◽

Survival Rates ◽

Foxtail Millet ◽

Negative Regulator ◽

Setaria Italica ◽

Mads Box ◽

Drought Stress Tolerance ◽

Genome Wide

MADS-box transcription factors play vital roles in multiple biological processes in plants. At present, a comprehensive investigation into the genome-wide identification and classification of MADS-box genes in foxtail millet (Setaria italica L.) has not been reported. In this study, we identified 72 MADS-box genes in the foxtail millet genome and give an overview of the phylogeny, chromosomal location, gene structures, and potential functions of the proteins encoded by these genes. We also found that the expression of 10 MIKC-type MADS-box genes was induced by abiotic stresses (PEG-6000 and NaCl) and exogenous hormones (ABA and GA), which suggests that these genes may play important regulatory roles in response to different stresses. Further studies showed that transgenic Arabidopsis and rice (Oryza sativa L.) plants overexpressing SiMADS51 had reduced drought stress tolerance as revealed by lower survival rates and poorer growth performance under drought stress conditions, which demonstrated that SiMADS51 is a negative regulator of drought stress tolerance in plants. Moreover, expression of some stress-related genes were down-regulated in the SiMADS51-overexpressing plants. The results of our study provide an overall picture of the MADS-box gene family in foxtail millet and establish a foundation for further research on the mechanisms of action of MADS-box proteins with respect to abiotic stresses.

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