scholarly journals Genome-Wide Analysis of MADS-Box Genes in Foxtail Millet (Setaria italica L.) and Functional Assessment of the Role of SiMADS51 in the Drought Stress Response

2021 ◽  
Vol 12 ◽  
Author(s):  
Wan Zhao ◽  
Li-Li Zhang ◽  
Zhao-Shi Xu ◽  
Liang Fu ◽  
Hong-Xi Pang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Survival Rates ◽  
Foxtail Millet ◽  
Negative Regulator ◽  
Setaria Italica ◽  
Mads Box ◽  
Drought Stress Tolerance ◽  
Genome Wide

MADS-box transcription factors play vital roles in multiple biological processes in plants. At present, a comprehensive investigation into the genome-wide identification and classification of MADS-box genes in foxtail millet (Setaria italica L.) has not been reported. In this study, we identified 72 MADS-box genes in the foxtail millet genome and give an overview of the phylogeny, chromosomal location, gene structures, and potential functions of the proteins encoded by these genes. We also found that the expression of 10 MIKC-type MADS-box genes was induced by abiotic stresses (PEG-6000 and NaCl) and exogenous hormones (ABA and GA), which suggests that these genes may play important regulatory roles in response to different stresses. Further studies showed that transgenic Arabidopsis and rice (Oryza sativa L.) plants overexpressing SiMADS51 had reduced drought stress tolerance as revealed by lower survival rates and poorer growth performance under drought stress conditions, which demonstrated that SiMADS51 is a negative regulator of drought stress tolerance in plants. Moreover, expression of some stress-related genes were down-regulated in the SiMADS51-overexpressing plants. The results of our study provide an overall picture of the MADS-box gene family in foxtail millet and establish a foundation for further research on the mechanisms of action of MADS-box proteins with respect to abiotic stresses.

Genome-wide identification of AP2/ERF transcription factors in mungbean (Vigna radiata) and expression profiling of the VrDREB subfamily under drought stress

2018 ◽  
Vol 69 (10) ◽  
pp. 1009 ◽  
Author(s):  
Abdullahi Muhammad Labbo ◽  
Maryam Mehmood ◽  
Malik Nadeem Akhtar ◽  
Muhammad Jawad Khan ◽  
Aamira Tariq ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Vigna Radiata ◽  
Critical Role ◽  
Drought Stress Tolerance ◽  
Legume Crop ◽  
Genome Wide ◽  
Responsive Element ◽  
Major Factors

Mungbean (Vigna radiata L.) is a valuable legume crop grown in tropical and subtropical areas of Asia. Drought is one of the major factors hindering its growth globally. APETALA2/ethylene-responsive element factor binding proteins (AP2/ERF) are an important family of plant-specific transcription factors (TFs) involved in drought-stress tolerance. We identified 71 AP2/ERF TFs in the mungbean genome by using bioinformatics tools and classified them into subfamilies: AP2 (16 members), ERF (22), RAV (2), DREB (30) and soloist (other proteins with no domain, 1). Members of DREB play a critical role in drought-stress tolerance. Ten-day-old mungbean plants cv. AZRI-06 were exposed to drought stress by complete withholding of water for 7 days. Root samples were collected from control and drought-stressed plants, and the expression pattern of 30 identified VrDREB genes was determined by qPCR. Most VrDREB genes exhibited differential expression in response to drought. Five genes (VrDREB5, VrDREB12, VrDREB13, VrDREB22, VrDREB30) were highly expressed under drought stress and might be considered excellent candidates for further functional analysis and for improvement of mungbean drought tolerance.

scholarly journals Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of  FT expression

2020 ◽  
Author(s):  
Reuben Tayengwa ◽  
Pushpa Sharma-Koirala ◽  
Courtney F. Pierce ◽  
Breanna E Werner ◽  
Michael M Neff
Keyword(s):  
Drought Stress ◽  
Transgenic Plants ◽  
Gene Family ◽  
Stress Tolerance ◽  
Flowering Time ◽  
Camelina Sativa ◽  
Negative Regulator ◽  
Wild Type ◽  
Late Flowering ◽  
Drought Stress Tolerance

Abstract Background The 29-member Arabidopsis AHL gene family is classified into three main classes based on nucleotide and protein sequence evolutionary differences. These differences include the presence or absence of introns, type and/or number of conserved AT-hook and PPC domains. AHL gene family members are divided into two phylogenetic clades, Clade-A and Clade-B. A majority of the 29 members remain functionally uncharacterized. Furthermore, the biological significance of the DNA and peptide sequence diversity, observed in the conserved motifs and domains found in the different AHL types, is a subject area that remains largely unexplored. Results Transgenic plants overexpressing AtAHL20 flowered later than the wild type. Transcript accumulation analyses showed that 35S:AtAHL20 plants contained reduced FT, TSF, AGL8 and SPL3 mRNA levels. Similarly, overexpression of AtAHL20’s orthologue in Camelina sativa, Arabidopsis’ closely related Brassicaceae family member species, conferred a late-flowering phenotype via suppression of CsFT expression. In addition, 35S:AtAHL20 seedlings exhibited suppressed hypocotyl length and enhanced water stress tolerance. However, overexpression of an aberrant AtAHL20 gene harboring a missense mutation in the AT-hook domain’s highly conserved R-G-R core motif abolished the late-flowering phenotype. Data from targeted yeast-two-hybrid assays showed that AtAHL20 interacted with itself and several other Clade-A Type-I AHLs which have been previously implicated in flowering-time regulation: AtAHL22, AtAHL27 and AtAHL29. Conclusion We showed via gain-function analysis that AtAHL20 is a negative regulator of FT expression, as well as other downstream flowering time regulating genes. A similar outcome in Camelina sativa transgenic plants overexpressing CsAHL20 suggest that this is a conserved function. Additionally, overexpression of AtAHL20 resulted in shorter hypocotyls and enhanced drought stress tolerance compared to wild-type plants. Our results demonstrate that AtAHL20 is a negative regulator of transition to flowering and hypocotyl elongation, but a positive regulator of drought stress tolerance.

scholarly journals Anthocyanins Are Key Regulators of Drought Stress Tolerance in Tobacco

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 139
Author(s):  
Valerio Cirillo ◽  
Vincenzo D’Amelia ◽  
Marco Esposito ◽  
Chiara Amitrano ◽  
Petronia Carillo ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Phenylpropanoid Pathway ◽  
Plant Responses ◽  
Physiological Mechanisms ◽  
Gas Exchanges ◽  
Drought Stress Tolerance ◽  
Leaf Gas Exchanges ◽  
Near Future

Abiotic stresses will be one of the major challenges for worldwide food supply in the near future. Therefore, it is important to understand the physiological mechanisms that mediate plant responses to abiotic stresses. When subjected to UV, salinity or drought stress, plants accumulate specialized metabolites that are often correlated with their ability to cope with the stress. Among them, anthocyanins are the most studied intermediates of the phenylpropanoid pathway. However, their role in plant response to abiotic stresses is still under discussion. To better understand the effects of anthocyanins on plant physiology and morphogenesis, and their implications on drought stress tolerance, we used transgenic tobacco plants (AN1), which over-accumulated anthocyanins in all tissues. AN1 plants showed an altered phenotype in terms of leaf gas exchanges, leaf morphology, anatomy and metabolic profile, which conferred them with a higher drought tolerance compared to the wild-type plants. These results provide important insights for understanding the functional reason for anthocyanin accumulation in plants under stress.

scholarly journals GhGLK1 a Key Candidate Gene From GARP Family Enhances Cold and Drought Stress Tolerance in Cotton

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiangna Liu ◽  
Teame Gereziher Mehari Mehari ◽  
Yanchao Xu ◽  
Muhammud Jaward Umer ◽  
Yuqing Hou ◽  
...  
Keyword(s):  
Drought Stress ◽  
Cold Stress ◽  
Candidate Gene ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Cold Treatment ◽  
Leaf Damage ◽  
Recurrent Parent ◽  
Drought Stress Tolerance ◽  
Wild Cotton

Drought and low-temperature stresses are the most prominent abiotic stresses affecting cotton. Wild cotton being exposed to harsh environments has more potential to cope with both biotic and abiotic stresses. Exploiting wild cotton material to induce resistant germplasm would be of greater interest. The candidate gene was identified in the BC2F2 population among Gossypium tomentosum and Gossypium hirsutum as wild male donor parent noted for its drought tolerance and the recurrent parent and a high yielding but drought susceptible species by genotyping by sequencing (GBS) mapping. Golden2-like (GLK) gene, which belongs to the GARP family, is a kind of plant-specific transcription factor (TF) that was silenced by virus-induced gene silencing (VIGS). Silencing of GhGLK1 in cotton results in more damage to plants under drought and cold stress as compared with wild type (WT). The overexpression of GhGLK1 in Arabidopsis thaliana showed that the overexpressing plants showed more adaptability than the WT after drought and cold treatments. The results of trypan blue and 3,3′-diaminobenzidine (DAB) staining showed that after drought and cold treatment, the leaf damage in GhGLK1 overexpressed plants was less as compared with the WT, and the ion permeability was also lower. This study suggested that the GhGLK1 gene may be involved in the regulation of drought and cold stress response in cotton. Our current research findings add significantly to the existing knowledge of cold and drought stress tolerance in cotton.

scholarly journals Genome-Wide Analysis of the MADS-Box Gene Family in Foxtail Millet (Setaria Italica L.) and Expression Analyses to Reveal Their Role During Drought Stress

2021 ◽  
Author(s):  
Xinlei Ma ◽  
Ningwei XU ◽  
Pengpeng Gu ◽  
Liqiang Du ◽  
Zhenqing Guo ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Gene Family ◽  
Expression Patterns ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Pcr Analysis ◽  
Induced Response ◽  
Mads Box ◽  
Element Analysis

Abstract MADS-box gene family is a key regulatory factor family, which controls vegetative growth, reproductive development and can be used to mediate abiotic stresses in many plants. However, Knowledge of this gene family is still limited in Setaria italica. In the present study, a total of 70 SitMADS genes were identified and renamed on the basis of the chromosomal distribution of the SitMADS genes. According to gene structure, conserved motif and phylogenetic feature, the 70 SitMADSs were classified into type-Ⅰ (Mα, Mβ, Mγ) and type-Ⅱ (MIKCC and MIKC*). All of the SitMADS genes were randomly distributed on nine chromosomes, and five tandem duplicated genes and 12 pairs of duplicated gene segments were detected in the SitMADS genes family. Synteny analysis provided a high homology between SitMADS genes and OsMADS genes. A cis-element analysis inferred that SitMADS genes, except for SitMADS23, possessed at least one drought stress response and ABA(Abscisic Acid)-induced response cis-element. Real-time quantitative PCR analysis was used to detect the expression patterns of SitMADS genes in various tissues and demonstrated that the genes responded drought stress and ABA treatments. SitMADS23, SitMADS42, SitMADS51, SitMADS52, SitMADS58 and SitMADS64 were highly expressed in PEG(Polyethylene glycol) and drought stress, which suggested its important role in drought stress response. SitMADS51, SitMADS63 and SitMADS64 seemed to be responsive to ABA hormone signaling, suggesting that they were involved in the ABA signaling pathways. This paper provided a deep insight into the evolutionary characteristics of SitMADS genes. The results provide comprehensive information for further analyses of the molecular functions of the MADS-box gene family in Setaria italica.

scholarly journals Genome-Wide Identification and Characterization of The MADS-Box Gene Family and Its Expression in The Various Developmental Stage and Stress Conditions in Foxtail Millet (Setaria Italica)

2021 ◽  
Author(s):  
Dili Lai ◽  
Jun Yan ◽  
Ailing He ◽  
Guoxing Xue ◽  
Hao Yang ◽  
...  
Keyword(s):  
Gene Duplication ◽  
Foxtail Millet ◽  
Grain Filling ◽  
Setaria Italica ◽  
Mads Box ◽  
Mads Box Genes ◽  
Specific Expression ◽  
Genome Wide ◽  
Duplication Events ◽  
Mads Box Gene

Abstract Foxtail millet (Setaria italica) is rich in nutrients and extremely beneficial to human health. We identified and comprehensively analyzed 89 MADS-box genes in the foxtail millet genome. According to the classification of MADS-box genes in Arabidopsis thaliana and rice, the SiMADS-box genes were divided into M-type (37) and MIKC-type (52). During evolution, the differentiation of MIKC-type MADS-box genes occurred before that of monocotyledons and dicotyledons. The SiMADS-box gene structure has undergone much differentiation, and the number of exons in the MIKC-type subfamily is much greater than that in the M-type subfamily. Analysis of gene duplication events revealed that MIKC-type MADS-box gene fragment duplication accounted for the vast majority of gene duplication events, and MIKC-type MADS-box genes played a major role in the amplification of SiMADS-box genes. Collinearity analysis showed highest collinearity between foxtail millet and maize MADS-box genes. Analysis of tissue-specific expression showed that SiMADS-box genes are highly expressed throughout the grain-filling process. Expression analysis of SiMADS-box genes under eight different abiotic stresses revealed many stress-tolerant genes, with induced expression of SiMADS33 and SiMADS78 under various stresses warranting further attention. Further, some SiMADS-box proteins may interact to cope with external stress. This study provides insights for MADS-box gene mining and molecular breeding of foxtail millet in the future.

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