A preliminary molecular analysis of phylogenetic and biogeographic relationships of New Zealand Thomisidae (Araneae) using a multi-locus approach

2013 ◽  
Vol 27 (6) ◽  
pp. 655 ◽  
Author(s):  
Philip J. Sirvid ◽  
Nicole E. Moore ◽  
Geoffrey K. Chambers ◽  
Kelly Prendergast
Keyword(s):  
New Zealand ◽  
Internal Transcribed Spacer ◽  
Nadh Dehydrogenase ◽  
Sequence Data ◽  
Histone H3 ◽  
Reliable Data ◽  
28S Rrna ◽  
Model Group ◽  
Nadh Dehydrogenase Subunit ◽  
Rrna Sequence

We tested competing theories on the origins of the New Zealand fauna using thomisid spiders as a model group. These theories can be broadly described as old and vicariant versus young and recent (dispersal). To test these theories, a phylogenetic analysis was undertaken based on cytochrome c oxidase subunit I (COI) and 28S rRNA sequence data, with smaller datasets (histone H3, nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 1 and a combined dataset) used to improve resolution of internal branches. The monophyly of New Zealand thomisid subfamilies and of individual taxa were also assessed using these data. Our data supports the separation of New Zealand clades from their Australian counterparts. Evidence of recent dispersal to New Zealand by Australian stephanopines combined with our proposed maximum divergence date of 5.3 mya indicates that the New Zealand thomisids are a younger lineage than previously suspected. Several other gene targets (internal transcribed spacer units 1 and 2, wingless and 18S rRNA) were examined but did not generate sufficient reliable data to contribute to the analysis. Corrected p-distance values for COI indicate that Sidymella angularis, a widely distributed and morphologically variable stephanopine species, is a single taxon. Three undescribed endemic species exhibited molecular and morphological distinctiveness from previously described New Zealand thomisids.

Molecular phylogeny of Australasian anopsobiine centipedes (Chilopoda : Lithobiomorpha)

2004 ◽  
Vol 18 (3) ◽  
pp. 235 ◽  
Author(s):  
Gregory D. Edgecombe ◽  
Gonzalo Giribet
Keyword(s):  
South Africa ◽  
New Species ◽  
New Zealand ◽  
New Caledonia ◽  
Sequence Data ◽  
Mitochondrial Protein ◽  
Molecular Data ◽  
12S Rrna ◽  
28S Rrna ◽  
16S Rna

Species assigned to the anopsobiine centipede genera Anopsobius Silvestri, 1899, and Dichelobius Attems, 1911, are widely distributed on fragments of the Gondwanan supercontinent, including temperate and tropical Australia, New Zealand, New Caledonia, the Cape region of South Africa, and southern South America. Phylogenetic relationships between Australasian and other Gondwanan Anopsobiinae are inferred based on parsimony and maximum likelihood analyses (via direct optimisation) of sequence data for five markers: nuclear ribosomal 18S rRNA and 28S rRNA, mitochondrial ribosomal 12S rRNA and 16S RNA, and the mitochondrial protein-coding cytochrome c oxidase subunit I. New molecular data are added for Anopsobius from South Africa and New Zealand, Dichelobius from New Caledonia, and a new species from Queensland, Australia, Dichelobius etnaensis, sp. nov. The new species is based on distinctive morphological and molecular data. The molecular phylogenies indicate that antennal segmentation in the Anopsobiinae is a more reliable taxonomic character than is spiracle distribution. The former character divides the Gondwanan clade into a 17-segmented group (Dichelobius) and a 15-segmented group (Anopsobius). Confinement of the spiracles to segments 3, 10 and 12 has at least two origins in the Gondwanan clade. The area cladogram for Dichelobius (Queensland (Western Australia + New Caledonia)) suggests a relictual distribution pruned by extinction.

Phylogeny and biogeography of the Australasian centipede Henicops (Chilopoda: Lithobiomorpha): A combined morphological and molecular approach

2006 ◽  
Vol 37 (3) ◽  
pp. 241-256 ◽  
Author(s):  
Donald Colgan ◽  
Gregory Edgecombe ◽  
Deirdre Sharkey
Keyword(s):  
New Zealand ◽  
Western Australia ◽  
New Caledonia ◽  
Sequence Data ◽  
Morphological Characters ◽  
Molecular Data ◽  
28S Rrna ◽  
Southeastern Australia ◽  
Lord Howe Island ◽  
Eastern Australia

AbstractThe lithobiomorph centipede Henicops is widely distributed in Australia and New Zealand, with five described species, as well as two species in New Caledonia and Lord Howe Island. Parsimony, maximum likelihood and Bayesian analyses of ca. 800 aligned bases of sequence data from 16S rRNA and 28S rRNA were conducted on a dataset including multiple individuals of Henicops species from populations sampled from different parts of species' geographic ranges, together with the allied henicopines Lamyctes and Easonobius. Morphological characters are included in parsimony analyses. Molecular and combined datasets unite species from eastern Australia and New Zealand to the exclusion of species from Western Australia, New Caledonia and Lord Howe Island. The molecular data favour these two geographic groupings as clades, whereas inclusion of morphology resolves New Caledonia, Lord Howe Island, southwest Western Australia and Queensland as successive sisters to southeastern Australia and New Zealand. The basal position of the Lord Howe Island species in the phylogeny favours a diversification of Australasian Henicops since the late Miocene unless the Lord Howe species originated in a biota that pre-dates the island. The molecular and combined data resolve the widespread morphospecies H. maculatus as paraphyletic, with its populations contributing to the geographic groupings New South Wales + New Zealand and Tasmania + Victoria.

Morphological and molecular characterization of Mermisnigrescens Dujardin, (Nematoda: Mermithidae) parasitizing the introduced European earwig (Dermaptera: Forficulidae) in New Zealand

2014 ◽  
Vol 89 (3) ◽  
pp. 267-276 ◽  
Author(s):  
B. Presswell ◽  
S. Evans ◽  
R. Poulin ◽  
F. Jorge
Keyword(s):  
New Zealand ◽  
18S Rrna ◽  
Sequence Data ◽  
18S Rrna Gene ◽  
Parasitic Nematodes ◽  
Rrna Gene ◽  
28S Rrna ◽  
Forficula Auricularia ◽  
Adult Females ◽  
European Earwig

AbstractParasitic nematodes of the family Mermithidae were found to be infecting the introduced European earwig Forficula auricularia (Dermaptera: Forficulidae) in Dunedin, South Island, New Zealand. Adult females were later collected from various garden plants while depositing eggs. These mermithid specimens were identified morphologically as Mermis nigrescens Dujardin, 1842. A genetic distance of 0.7% between these specimens and a M. nigrescens isolate from Canada (18S rRNA gene), suggests that they have diverged genetically, but there are currently no available comparable sequences for the European M. nigrescens. Two additional nuclear fragments were also amplified, the 28S rRNA and the ribosomal DNA first internal transcribed spacer (ITS1), providing a basis for future studies. Bearing in mind the morphological similarity with other reported M. nigrescens and the lack of sequence data from other parts of the world, we retain the name M.nigrescens, and suggest that the species may be found to represent a complex of cryptic species when more worldwide data are available. Herein, we present a brief description of the post-parasitic worms and adult females, along with an inferred phylogeny using 18S rRNA gene sequences.

Analysis of Genetic Variation in Ribosomal DNA Internal Transcribed Spacer and the NADH Dehydrogenase Subunit 4 Mitochondrial Genes of the Onchocerciasis VectorSimulium ochraceum

2006 ◽  
Vol 43 (4) ◽  
pp. 701-706 ◽  
Author(s):  
Mario A. Rodríguez-pérez ◽  
Claudia A. Núñez-gonzález ◽  
Cristian Lizarazo-ortega ◽  
Alejandro Sánchez-varela ◽  
Michael C. Wooten ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Nadh Dehydrogenase ◽  
Mitochondrial Genes ◽  
Nadh Dehydrogenase Subunit

Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China

Phytotaxa ◽  
2016 ◽  
Vol 269 (2) ◽  
pp. 90 ◽  
Author(s):  
ZHUO DU ◽  
XIN-LEI FAN ◽  
KEVIN D. HYDE ◽  
QIN YANG ◽  
YING-MEI LIANG ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Histone H3 ◽  
Elongation Factor ◽  
Betula Platyphylla ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range ◽  
Dieback Disease ◽  
Elongation Factor 1

Diaporthe species are common pathogens, endophytes, or saprobes on a wide range of hosts. During our investigation of forest pathogens, we made collections of Diaporthe species associated with canker and dieback disease of Betula platyphylla and B. albosinensis in Sichuan and Shaanxi provinces in China. Diaporthe betulae sp. nov. and D. betulicola sp. nov. are introduced in this paper, with illustrations, descriptions and support from analysis of ribosomal DNA internal transcribed spacer (ITS), calmodulin (CAL), histone H3 (HIS), translation elongation factor 1-α (TEF1-α) and beta-tubulin (TUB2) sequence data. Diaporthe betulae is characterized by hyaline, ellipsoidal, aseptate, biguttulate, 8.5–11 × 3–4 µm alpha conidia. Diaporthe betulicola is characterized by pycnidial stromata with a single locule with one ostiole per disc. Alpha conidia are hyaline, oblong, aseptate, lack guttules and 9.9–14.7 × 1.3–2.5 µm, and beta conidia are hyaline, spindle-shaped, curved, aseptate and 17–24 × 0.7–1.2 µm.

scholarly journals Onchocerca Flexuosa. sp. (Nematoda: Filarioidea) in Japanese Wild Sika Deer (Cervus Nippon): Pathological and Molecular Identification of Intradermal Worms

2021 ◽  
Author(s):  
Hoda Abd elatieff ◽  
Eman Bazaa ◽  
Shahinaz Hussin ◽  
Ituto Yamamoto ◽  
Tokuma Yanai ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Ribosomal Rna ◽  
Nadh Dehydrogenase ◽  
Sika Deer ◽  
Cervus Nippon ◽  
The Body ◽  
28S Rrna ◽  
Nadh Dehydrogenase Subunit ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Vector Borne

Abstract More than thirty species of the genus Onchocerca (Nematoda; Filarioidea) were responsible for onchocercosis (a vector-borne parasitic disease) of wild and domestic ungulates. “Between 2016 and 2017”, the presence of Onchocerca flexuosa (Wedl, 1856) was detected and investigated in 17 Japanese Sika deer (Cervus nippon) captured in Gifu and Shiga Prefectures, Japan. The worms were found within characteristics subcutaneous nodules dispersed in different regions of the body of all 17 deer. The all collected nodules were examined stereo-microscopically. The parasites were extracted from the nodules and identified through morphological and histopathological examinations. Molecular identification through sequencing of the following genes; internal transcribed spacer subunit 2 (ITS2)–28S ribosomal RNA (28S rRNA), cytochrome c oxidase subunit 1 (cox1) and mitochondrially encoded NADH dehydrogenase subunit 2 (NAD2) were performed. The histopathological, molecular and phylogenetic analysis demonstrated that, the filarial nematode isolated from Gifu and Shiga Prefectures in Japan is O. flexuosa. This is the first report about presence of O. flexuosa in Japanese Sika deer (Cervus nippon) in Gifu and Shiga Prefectures.

scholarly journals A comparison of populations of Armillaria hinnulea in New Zealand and Australia

2008 ◽  
Vol 61 ◽  
pp. 41-47 ◽  
Author(s):  
T.D. Ramsfield ◽  
M.W.P. Power ◽  
G.S. Ridley
Keyword(s):  
New Zealand ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Its Region ◽  
The Other ◽  
Dna Sequence Data ◽  
Nothofagus Forests ◽  
Preliminary Study ◽  
Decaying Wood ◽  
The Relationship

Armillaria hinnulea has been recorded from the northwest quadrant of the South Island of New Zealand Tasmania and Australia In New Zealand A hinnulea is rare being restricted to decaying wood in South Island Nothofagus forests A preliminary study of the relationship between the populations of A hinnulea was performed using DNA sequence data from the internal transcribed spacer (ITS) region of the ribosomal DNA Two major ITS clades were observed each of which included representative sequences from Australian collections Within one ITS clade one Australian sequence was 100 identical with the New Zealand isolates while in the other ITS clade eight New Zealand isolates representing five locations were 100 identical but the Australian sequence differed by five base substitutions and was in a separate subclade These results suggest at least two introductions of A hinnulea have occurred one relatively recently and one in the more distant past

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