Validation of the use of thin-sectioned Otoliths for determining the age and growth of Golden Perch, Macquaria ambigua (Perciformes: Percichthyidae), in the Lower Murray-Darling Basin, Australia

1992 ◽  
Vol 43 (5) ◽  
pp. 1103 ◽  
Author(s):  
JR Anderson ◽  
AK Morison ◽  
DJ Ray

Golden perch, Macquaria ambigua, from the Murray-Darling Basin were aged by using transverse thin sections of their sagittal otoliths. Samples from 889 fish were obtained from riverine and lacustrine habitats and from wild and stocked populations. Error in the precision of age estimates (calculated as the mean percentage error of the independent age estimates of four readers) was 5.6% (3.9% after allowing for discrepancies in relation to the annual mark on the edge of the otolith). Validation was accomplished by using a combination of analysis of the progression of modes in length-frequency distributions, qualitative and quantitative marginal-increment analysis, and analysis of age estimates of fish from populations with a known stocking history. The technique was validated for fish up to 8 years of age (455-545 mm total length, 1695-3988 g total weight), and the greatest recorded age was 16 years (530-600 mm total length, 2607-4050 g total weight). Annual marks become visible in otolith sections in most fish of all ages in October, and 1 October was designated as the birth date. A description of our method of reading sections of golden perch otoliths, including recognition of false annual marks, is given. Otolith length, width and thickness increased linearly with fish length and with loglo(fish age), whereas otolith weight increased linearly with fish age and exponentially with fish length. The continuous growth of the otoliths and the consistency in the appearance of annual marks support the accuracy of estimates up to the maximum recorded age. The mean length-at-age and the parameters of the length-weight relationship were estimated. The von Bertalanffy growth parameters were also estimated (L∞ =507 mm, to=0.420 years, K=0.454). No significant differences were found in growth rates or length-weight relationships between males and females. However, growth (particularly in weight) was highly variable among sites and years, and slow-growing 5-year-olds may be shorter than fastgrowing 1-year-olds. Ages were estimated for a sample of 86 golden perch caught between 1949 and 1951 but a comparison of growth rates between these and more recent collections was inconclusive.

1992 ◽  
Vol 43 (5) ◽  
pp. 983 ◽  
Author(s):  
JR Anderson ◽  
AK Morison ◽  
DJ Ray

Transverse thin sections (0.5 mm thick) of sagittal otoliths from 290 Murray cod up to 1400 mm in total length and 47.3 kg in weight were used to establish the age and growth of cod in the lower Murray-Darling Basin, including comparisons of recent (1986-91) and past (1949-51) growth rates and growth in different waters. The maximum estimated age was 48 years. Quantitative and qualitative analysis of the seasonal changes in otolith marginal increments showed that annuli in fish of all ages were laid down each spring, and 1 October was assigned as the birthday. The thin-sectioning method was validated by comparing age estimates for 55 Murray cod from Lake Charlegrark (age 0-21 years), which had been validated by using burnt and polished half-otoliths. The new method had an accuracy of 96.4% and it offers major advantages in ease of preparation, reading, and batch-handling of large numbers of otoliths. The precision of the method, estimated as an average error for four readers, was 5.4% (3.0% after ignoring discrepancies in relation to annuli on otolith edges). There was a linear relationship between otolith weight and fish age and an exponential relationship between otolith weight and fish length. Both otolith length and otolith width reached an asymptote at about 15 years, when fish length also approached its maximum. However, otolith thickness continued to increase throughout the life of the fish and, after about 15 years, contributed most to the increase in otolith weight. This confirmed that otoliths continued to grow in thickness and that annuli were laid down throughout life, and that cod could be aged reliably to the maximum age. The annulus pattern is very clear and distinct, and the reading techniques are fully described, including recognition of 'larval' and 'false' rings. Various differences were found in the growth rates, and the length-weight relationships for males and females, for cod caught in 1986-91 and those caught in 1949-51, and various subpopulations are discussed. The von Bertalanffy growth parameters (all individuals combined) were estimated at L∞ = 1202 mm, k=0.108 and t0= -0.832. The availability of a reliable ageing method provides the first opportunity to determine year of birth and thus to examine the age structure of populations and to effectively manage cod populations that have declined in abundance.


2001 ◽  
Vol 52 (4) ◽  
pp. 637 ◽  
Author(s):  
A. K. Morison ◽  
K. R. Rowling

Age and growth of 5678 redfish, collected during 1991–98 from Australia’s South East Fishery, were estimated from thin sections of otoliths. A maximum age of 44 years was recorded for a 30 cm (fork length) female, but 80%of females in the commercial catch were <10 years, and 80%of males were <13 years. The largest was a 34 cm female estimated to be 36 years old. Repeated age estimates of a subsample revealed an average error of 3.79%. There was significant variation in the mean length-at-age among years, and there were significant effects for age*year, age*sex, age*region, region*year, and sex*region*year interactions. Assessments of the fishery have assumed a single stock, because tagging results from the 1980s indicate movement of redfish along the coast. This study found consistent differences in sex ratio and growth rate between regions, which indicate some structuring within the population. However, the differences in growth rates are not consistent among years and could not be explained by differences in depths fished, suggesting a more dynamic situation than spatially segregated stocks. Estimates of natural mortality ranged from 0.07 to 0.11 year–1 and differed between regions.


1997 ◽  
Vol 54 (1) ◽  
pp. 126-136 ◽  
Author(s):  
H A Williams ◽  
M K Lowe

Two methods were used to estimate the age and growth of four Hawaiian deep slope fishes. Otolith daily increment width estimates from radial sections of sagittal otoliths were integrated over the otolith radius to approximate age by (i) dividing interval length (micrometres) by mean increment width (micrometres per day) and summing this quotient for consecutive 500- µm radial intervals (S. Ralston and H.A. Williams. 1989. Fish. Bull. 87: 1-16) and (ii) fitting increment width (y) versus otolith radius (x) to a Gompertz rate curve and calculating the inverse integral of this curve (M.K. Smith and E. Kostlan. 1991. Fish. Bull. 89: 461-472). The methods also differ in the way the age-length key is generated. Ralston and Williams' method uses integrated age versus a regression-based estimate of fish length at 500- µm otolith radial intervals; Smith and Kostlan's method uses estimated age versus actual fish length. Neither method of integration produced significantly different age estimates; however, the use of regression-based fish length estimates instead of sampled fish lengths resulted in significant differences between estimated growth curves. The minimum number of daily increment width estimates required as a function of otolith radius was calculated, on the basis of observed microincrement width variation.


2013 ◽  
Vol 70 (9) ◽  
pp. 1396-1401 ◽  
Author(s):  
Joshua D. Dub ◽  
Rebecca A. Redman ◽  
David H. Wahl ◽  
Sergiusz J. Czesny

Age estimates from otolith morphometrics represent a rapid and objective alternative to traditional ageing techniques though use has been limited to marine and short-lived freshwater species. We utilized random forest analysis with otolith mass, total length, and several temporal and spatial predictor variables to assess variable importance and accuracy of age estimates for age-0 through age-11 yellow perch (Perca flavescens) in southwestern Lake Michigan. Accuracy of age predictions decreased with increasing age as 95% of juvenile (age-0 through age-2) ages were predicted correctly compared with 55% for adults (age-3 through age-11). Precision of age estimates within 1 year of reader-assigned age were high for both juvenile and adult yellow perch at 100% and 86%, respectively. Otolith mass was the most important predictor variable; however, substantial overlap existed among adult ages. Random forest analysis utilizing otolith mass, total length, and other pertinent predictor variables represents an applicable tool to reduce subjectivity and resource expenditure while providing accurate age estimates for juvenile and short-lived fishes.


Author(s):  
Gülşen Uzun Gören ◽  
Sedat Karayücel ◽  
Birol Baki

In this study, it was aimed to determine there and fecundity by adapting crayfish (Astacus leptodactylus) obtained from Bafra Fish Lakes (Ulugöl-Samsun) in culture conditions. Crayfish obtained in November 2016 were stocked in tanks and egg and morphometric characteristics of female crayfish with carrying eggs were determined. Correlation analysis were used for the relationship between the determined parameters. The mean total weight and total length of crayfish were 41.79±3.00g, 10.72±0.22cm, respectively whereas the mean number and diameter of eggs were 156.40±33.24 and 2.50±0.12mm. There were positive strong relationship between total length and total weight of crayfish negative relationship was found between egg diameter and total number of eggs, egg diameter and total weight of eggs and egg diameter and number of eggs for per unit of live weight.


1970 ◽  
Vol 44 (3) ◽  
pp. 377-379 ◽  
Author(s):  
Bimal Kanta Saha ◽  
M Rafiqul Islam ◽  
Aparna Saha ◽  
M Altaf Hossain

Some aspects of the reproductive biology viz. sex ratio, fecundity and reproductive periodicity of Amblypharyngodon mola were studied during March 2004 through February 2005 in Netrakona. Sex ratio was found to be 1:2.03. Fecundity varied from 1,291 to 12,797 with a mean value 5,751.46 ± 3,321.73 eggs. The relationships between Fecundity (F) and Total Length (TL), Standard Length (SL), Total Weight (TW), Gonadal length (GL) and Gonadal Weight (GW) were calculated and found to be highly significant. The mean of ova diameter was 1.4 ± 0.35mm. Different methods used to study the reproductive periodicity showed that the periodicity of A. mola starts from March and continues up to August with a peak in May. Key words: Reproductive biology, Reproductive periodicity, Amblypharyngodon mola. DOI: 10.3329/bjsir.v44i3.4414 Bangladesh J. Sci. Ind. Res. 44(3), 377-379, 2009   


Author(s):  
I. Mohamad ◽  
F.A. Bhat ◽  
M.H. Balkhi ◽  
T.H. Shah ◽  
B.A. Bhat ◽  
...  

Background: Fecundity is an important parameter in fisheries that predicts the reproductive capability of fish stock. Information on the fecundity of Common carp is helpful in estimating the amount of offspring produced in spawning season, which is essential for the stock assessment of the species. Assessment of the fecundity of a fish is essential for evaluating the commercial potentialities of its stock, life history, practical culture and actual management of the fishery. Common carp is a commercially important fish which has the potential to meet the demand of food in the country. Scanty of data is available on fecundity parameter of common carp in Kashmir waters. Therefore, this research work aims to estimate the fecundity of Common carp collected from the Dal lake of Kashmir. Methods: The present study aims to estimate the fecundity of Common carp, [Cyprinus carpio var. communis] existing in Dal Lake, Kashmir. The 30 fish samples were collected once every month from the month of January 2018 to December 2018. Fishes were brought to Fisheries Resource Management (FRM), Faculty of Fisheries, SKUAST-K Laboratory for the estimation of total length, weight of fishes and weight of ovaries. Gravimetric and actual counting method was used for the estimation of fecundity. Result: It was observed that the number of eggs varied from 16650 (for a fish with total length 180mm and total weight 120 g) to 129000 (for a fish with total length 430 mm and total weight 1300 g. The mean absolute fecundity was recorded as 68864.07± 6563.59 for a fish with a mean total length of 301.43 ± 13.93 mm and mean total weight of 435.1 ± 66.51 g. The relative fecundity ranged from 99.2-240.8 and the mean relative fecundity was 185.96. Significant positive correlation was found between fish weight and absolute fecundity. (r= 0.907, p less than 0.01), fish length and absolute fecundity (r= 0.976, p less than 0.01), Ovary weight and absolute fecundity (r= 0.998, p less than 0.01). Relative fecundity showed a significant negative correlation with weight, length and absolute fecundity (r= -0.747, p less than 0.01; r= -0.419, p less than 0.05 and r= -0.460, p less than 0.05 respectively.


1992 ◽  
Vol 22 (10) ◽  
pp. 1562-1567 ◽  
Author(s):  
Fernand Pagé ◽  
Bady Badibanga ◽  
Annie Sauvesty ◽  
Colette Ansseau

A method for rapidly estimating the rootlet volume and mass in sugar maple groves was developed in relation with the rootlet size. Soil samples were obtained with corers and cut in 2-cm thick slices. The number of rootlets of diameter classes <0.3, 0.3–0.5, 0.5–0.8, and 0.8–1.0 mm were determined under microscope (× 150) on the upper surface of slices. Rootlet surface for each class was measured on thin sections of soil, with an optic microscope connected to a digitalizing board. Mean rootlet surface [Formula: see text] showed a small standard deviation within each diameter class. The rootlet surface (S) on a surface of soil St can be estimated as [Formula: see text], where N is the number of rootlets observed in nc fields of observation, each field having a surface So. If two soil slices are located at depths h1 and h2, respectively, and if the rootlet surfaces of those slices are S1 and S2, the rootlet volume V of the first slice corresponds approximately to the volume of a truncated cone, and can be calculated as V = [S1 + S2 + (S1S2)0,5] (h2–h1)/3. The rootlet density (g•cm−3 of root) was also determined for each diameter class. The standard deviation from [Formula: see text], the mean density for each diameter class was small. Thus, the rootlet mass M was determined as [Formula: see text]. Estimated and real rootlet volume and mass values were compared. A difference of about 10% was found between estimated and real values.


2008 ◽  
Vol 90 (6) ◽  
pp. 477-482 ◽  
Author(s):  
S Devaraj ◽  
SR Dodds

INTRODUCTION Some studies have considered abdominal aortas of 2.6–2.9 cm diameter (ectatic aortas) at age 65 years as being abnormal and have recommended surveillance, whereas others have considered these normal and surveillance unnecessary. It is, therefore, not clear how to manage patients with an initial aortic diameter between 2.6–2.9 cm detected at screening. The aim of this study was to evaluate growth rates of ectatic aortas detected on initial ultrasound screening to determine if any developed into clinically significant abdominal aortic aneurysms (AAAs; > 5.0 cm) and clarify the appropriate surveillance intervals for these patients. PATIENTS AND METHODS Data were obtained from a prospective AAA screening programme which commenced in 1992. The group of patients with initial aortic diameters of 2.6–2.9 cm with a minimum of 1-year follow-up were included in this study (Group 2). This was further divided into two subgroups (Groups 3a and 3b) based on a minimum follow-up interval obtained from outcome analysis. Mean growth rate was calculated as change in aortic diameter with time. The comparison of growth rates in Groups 3a and 3b was performed using the t-test. The number and proportion of AAAs that expanded to ≥ 3.0 cm and ≥ 5.0 cm in diameter were also calculated. RESULTS Out of 999 patients with AAA ≥ 2.6 cm with minimum 1-year follow-up, 358 (36%) were classified as ectatic aortas (2.6–2.9 cm) at initial ultrasound screening with the mean growth rate of 1.69 mm/year (95% CI, 1.56–1.82 mm/year) with a mean follow-up of 5.4 years. Of these 358 ectatic aortas, 314 (88%) expanded into ≥ 3.0 cm, 45 (13%) expanded to ≥ 5.0 cm and only 8 (2%) expanded to ≥ 5.5 cm over a mean follow-up of 5.4 years (range, 1–14 years). No ectatic aortas expanded to ≥ 5.0 cm within the first 4 years of surveillance. Therefore, the minimum follow-up interval was set at 4 years and this threshold was then used for further analysis. The mean growth rate in Group 3a (< 5.0 cm at last scan) was 1.33 mm/year (95% CI, 1.23–1.44 mm/year) with a mean follow-up of 7 years compared to Group 3b (≥ 5.0 cm at last scan) with the mean growth rate of 3.33 mm/year (95% CI 3.05–3.61 mm/year) and a mean follow-up of 8 years. The comparison of mean growth rates between Groups 3a and 3b is statistically significant (t-test; T = 13.00; P < 0.001). CONCLUSIONS One-third of patients undergoing AAA screening will have ectatic aortas (2.6–2.9 cm) and at least 13% of these will expand to a size of ≥ 5.0 cm over a follow-up of 4–14 years. A threshold diameter of 2.6 cm for defining AAAs in a screening programme is recommended and ectatic aortas detected at age 65 years can be re-screened at 4 years after the initial scan. A statistically significant difference was found in the growth rates of ectatic aortas with minimum 4 years follow-up, expanding to ≥ 5.0 cm compared to those less than 5.0 cm at last surveillance scan. Further studies are required to test the hypothesis of whether growth rate over the first 4 years of surveillance will identify those who are most likely to expand to a clinically significant size (> 5.0 cm).


2018 ◽  
Vol 59 (1) ◽  
pp. 97-110 ◽  
Author(s):  
Mado Kotsiri ◽  
Ioannis E. Batjakas

The otoliths of the Atlantic bonito, Sarda sarda (Bloch, 1793), were examined with the aim to estimate the age and growth of the species in the eastern Mediterranean Sea and to reveal possible relationships between otolith shape or size and age. All specimens used in this study, ranging from 7.2 to 70.4 cm in fork length and from 20 to 4889 g in total weight, were caught in the Aegean and Ionian Seas during the period 1997-2010. Otolith morphometry was studied using image analysis techniques for all intact sagittae ranging in weight from 0.6 to 11.3 mg and four shape indices were calculated. No statistical significant differences between left and right otolith morphometric variables were found. The age of fish was estimated by counting the pairs of opaque and translucent bands in transversal thin sections of otoliths. The estimated ages ranged from 0+ to 7 years and the von Bertalanffy growth parameters were determined \((L∞=79.9 cm, k=0.261 and to=-1.230 years)\). The examination of the type of growth bands at the outside margin of each otolith per month showed that one translucent band is formed annually during the cold season. The results revealed statistically significant relationships between otolith morphometric variables and fish length or age. Among the variables, otolith weight was the one that showed the highest correlation with age (R=0.77). Therefore, otolith weight could represent a valuable criterion for age estimation in Atlantic bonito that is objective, economic and easy to perform compared to annuli counting method in hard parts.


Sign in / Sign up

Export Citation Format

Share Document