8 ANTI-MÜLLERIAN HORMONE AT WEANING AND BREEDING AS A PREDICTOR OF BEEF HEIFER FERTILITY

2017 ◽  
Vol 29 (1) ◽  
pp. 111
Author(s):  
R. W. Rorie ◽  
H. R. Newberry ◽  
T. D. Lester ◽  
M. Acharya ◽  
C. R. Hansen
Keyword(s):  
Breeding Season ◽  
Prostaglandin F2α ◽  
Oestrous Cycle ◽  
Transrectal Ultrasonography ◽  
Beef Heifers ◽  
Subsequent Fertility ◽  
Chi Squared ◽  
Fetal Size ◽  
Beef Heifer ◽  
Conception Date

This study examined the relationship between serum anti-Müllerian hormone (AMH) concentration at weaning and breeding to determine if either or both measures could predict subsequent fertility of beef heifers. Blood was collected from 71 Angus based heifers at ~7 and 14 months of age and serum stored frozen (−20°C) until analysis for AMH, using bovine AMH ELISA kits (AL115; Ansh Laboratories, Webster, TX, USA). Before breeding, transrectal ultrasonography was used to determine cyclic status of heifers. Heifers received 25 mg of prostaglandin F2α (PGF) and those detected in oestrus were AI ~12 h after detected oestrus. Heifers not detected in oestrus after 7 days received a second PGF injection and oestrus detection and AI continued for 4 days. Ten days later, the heifers were exposed to fertile bulls for a 45-days breeding season. At 50 to 60 days after insemination, transrectal ultrasonography was used to identify pregnant heifers and to confirm conception date based on fetal size. At 60 days after bull removal, ultrasonography was performed to determine pregnancy in heifers conceiving during the breeding season and confirm continuing pregnancy in heifers previously identified as pregnant. Based on fetal size at ultrasonography, the oestrous cycle after initiation of breeding when conception occurred was estimated. For comparison, AI pregnancies were considered cycle 1, and pregnancies initiated during subsequent 21-day intervals of the breeding season were classified as cycles 2, 3, and 4, respectively. For analysis, frequency distribution was used to assign serum AMH concentration measured at weaning, breeding, and the change from weaning to breeding, into quartiles. Comparisons were then made via Chi-squared analysis for heifers in each quartile that were cyclic at synchronization, detected in oestrus, conceived after AI, pregnant at the end of the breeding season, and the estimated cycle that conception occurred. Comparison of heifers based on serum AMH quartiles at weaning failed (P ≥ 0.35) to detect any effect of AMH on subsequent heifer cyclicity at breeding, oestrous response after synchronization, AI conceptions, overall pregnancy rate, or estimated oestrous cycle of the breeding season when conception occurred. Based on AMH concentration at breeding, heifers in the lowest quartile (Q1) had a lower (10%; P = 0.02) AI conception rate than heifers in other quartiles (43 to 73%), and conceived at a later oestrous cycle (P = 0.03) in the breeding season. Comparison of heifers based on the change in AMH concentrations from weaning to breeding revealed that none of the heifers in the lowest quartile (Q1) conceived after AI, compared with 80% in the highest quartile (Q4; P < 0.001). Heifers in the lowest quartile also conceived at a later oestrous cycle in the breeding season than heifers in the other quartiles (P = 0.01). Results indicate that either AMH concentration at breeding or the change in AMH from weaning to breeding can identify beef heifers more likely to conceive to AI and to conceive early in the breeding season.

scholarly journals Beef heifer fertility: importance of management practices and technological advancements

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Sarah E. Moorey ◽  
Fernando H. Biase
Keyword(s):  
Breeding Season ◽  
Production Systems ◽  
Transcript Abundance ◽  
Antral Follicle ◽  
Economic Losses ◽  
Beef Heifers ◽  
Management Procedures ◽  
Beef Heifer ◽  
Fertility Potential ◽  
Cow Calf

Abstract The development of replacement heifers is at the core of cow-calf beef production systems. In 2020, the USDA, National Agricultural Statistics Service reported 5.771 million beef heifers, 500 pounds and over, are under development for cow replacement. A compilation of data from several studies indicate that between 85% and 95% of these heifers will become pregnant in their first breeding season. Several thousands of heifers being raised for replacement may not deliver a calf on their first breeding season and result in economic losses to cow-calf producers. Many management procedures have been developed to maximize the reproductive potential of beef heifers. Such approaches include, but are not limited to the following: nutritional management for controlled weight gain, identification of reproductive maturity by physiological and morphological indicators, and the implementation of an estrous synchronization program. The implementation of management strategies has important positive impact(s) on the reproductive efficiency of heifers. There are limitations, however, because some heifers deemed ready to enter their first breeding season do not become pregnant. In parallel, genetic selection for fertility-related traits in beef heifers have not promoted major genetic gains on this particular area, most likely due to low heritability of female fertility traits in cattle. Technologies such as antral follicle counting, DNA genotyping and RNA profiling are being investigated as a means to aid in the identification of heifers of low fertility potential. To date, many polymorphisms have been associated with heifer fertility, but no DNA markers have been identified across herds. Antral follicle count is an indication of the ovarian reserve and is an indicator of the reproductive health of a heifer. We have been working on the identification of transcriptome profiles in heifers associated with pregnancy outcome. Our current investigations integrating protein-coding transcript abundance and artificial intelligence have identified the potential for bloodborne transcript abundance to be used as indicators of fertility potential in beef heifers. In summary, there is an ongoing pressure for reducing costs and increasing efficiency in cow-calf production systems, and new technologies can help reduce the long-standing limitations in beef heifer fertility.

12 Artificial insemination and embryo transfer results in ewes during a long daylength period

2021 ◽  
Vol 33 (2) ◽  
pp. 113
Author(s):  
T. Mittleider ◽  
S. Collins ◽  
P. Gibbons ◽  
J. Gibbons
Keyword(s):  
Embryo Transfer ◽  
Field Trial ◽  
Prostaglandin F2α ◽  
Late Summer ◽  
Fertilization Rate ◽  
Pregnancy Rates ◽  
Transrectal Ultrasonography ◽  
Chi Squared ◽  
Reproductive Techniques ◽  
Successful Approach

Sheep are polyestrous, short-day breeders (∼11h of daylength), and exhibit oestrus approximately every 16–17 days during the breeding season, usually in late September to late December in the Northern Hemisphere. Progressive sheep producers often use assisted reproductive techniques such as laparoscopic AI and ovarian hyper-stimulation, embryo collection, and embryo transfer (ET) to increase genetic gain, and strive to have early December lambs to target specific show markets, which dictates AI or ET during the late summer. This field trial compared pregnancy rates following AI or ET in July and August (∼14h of daylength) in southwest Virginia (36–38′12″ N). Ewes (AI, n=83; ET recipients, n=33) were synchronized using a modified Ovsynch protocol involving intravaginal progesterone implants for 14 days, prostaglandin F2α (intramuscular) 48h before expected oestrus, and PG600 (IM) and gonadotrophin-releasing hormone (intramuscular) 52 to 54h and 16h before AI, respectively. Ewes were subjected to AI (frozen/thawed semen) regardless of whether they displayed signs of oestrus, and ewes selected as embryo recipients were subjected to a similar protocol but instead received 1 or 2 embryos (based upon the number of viable embryos produced per embryo donor) 6 days following the AI of the embryo donors. Ovarian hyper-stimulation of the embryo donors (n=13) was enabled by twice-daily FSH injections [totalling 350–455IU of Folltropin V (10–13mL)] for the 4 days before AI. Six days following AI, embryos were recovered surgically from the embryo donors (n=13) and yielded an average (±s.e.m.) of 6.6±1.2 total ova, 4.7±1.1 transferable quality embryos, and 1.9±0.8 unfertilized ova per collection. Pregnancy was detected using transrectal ultrasonography at ∼30 days of gestation and the pregnancy rates were analysed using Chi-squared. There was a tendency (P=0.092) for more pregnancies to be established following ET (22/33; 66%) compared with AI (41/83; 49%). There was no statistical relationship between AI ewes or ET recipient ewes that became pregnant relative to whether they displayed signs of oestrus or not. Embryo transfer was a more successful approach to produce pregnancies in ewes compared with AI during long daylength periods in this field trial. Further, ova from hyper-stimulated embryo donor ewes experienced a very high fertilization rate. Future studies will evaluate the ova capability directly via laparoscopic aspiration of ovarian follicles and IVF and evaluation of hyper- and non-hyper-stimulated follicular waves (using transrectal ultrasonography) and endocrine dynamics during different long and short daylength periods. Extending the opportunity to generate embryos and offspring regardless of daylength will assist aggressive sheep producers in reaching their reproductive, financial, and genetic goals.

scholarly journals Effects of prolonging the interval from progestin removal to prostaglandin F2α injection from 16 to 17 days in a long-term estrus synchronization protocol in beef heifers

2021 ◽  
Author(s):  
N Oosthuizen ◽  
G D Melo ◽  
G E Seidel ◽  
R L Stewart ◽  
L Rowden ◽  
...  
Keyword(s):  
Prostaglandin F2α ◽  
Fixed Time ◽  
Transrectal Ultrasonography ◽  
Dominant Follicle ◽  
Beef Heifers ◽  
Treatment Groups ◽  
Follicle Diameter ◽  
Randomized Design ◽  
Angus Heifers

Abstract To determine effects of delaying the injection of prostaglandin F2α (PGF) and fixed-time artificial insemination (TAI) in the 14-d CIDR-PG protocol, 1,049 Angus heifers at six locations were enrolled in a completely randomized design. Within location heifers were randomly assigned to one of two treatment groups: 1) PG16 (n = 518), heifers received a controlled internal drug release (CIDR) insert on d 0 for 14 d, a 25-mg injection of PGF 16 d after CIDR removal (Day 30), and a 100-µg injection of gonadotropin-releasing hormone concurrent with TAI 66 ± 2 h later; or 2) PG17 (n = 531), heifers were treated the same as PG16, however, PGF was administered 17 d after CIDR removal (Day 31), and heifers were TAI 66 ± 2 h later. Estrus detection patches were applied to a subset (n = 482) of heifers at the time of PGF administration and were examined for activation at TAI. Dominant follicle diameter was determined via transrectal ultrasonography at PGF administration and TAI in a subset of heifers (n = 116). Transrectal ultrasonography was performed to determine pregnancy rates to TAI (PR/AI) between 30 and 45 d after TAI. Estrus expression prior to TAI differed by treatment where PG17 heifers had greater (P &lt; 0.01) expression of estrus than PG16 heifers (57.8 ± 6.1 vs. 43.4 ± 6.1%, respectively). Nevertheless, dominant follicle diameters at PGF and at TAI were similar (P ≥ 0.59) between PG16 and PG17 heifers. In addition, PR/AI did not differ (P = 0.29) between PG16 and PG17 treatments (50.5 ± 3.2 vs. 45.7 ± 3.1%, respectively). Results of this experiment indicate that delaying the injection of PGF and TAI in the 14-day CIDR-PG protocol increased estrus expression prior to TAI yet did not improve fertility in beef heifers.

scholarly journals PSII-29 Effects of delayed injection of prostaglandin F2α and TAI in the 14-d CIDR-PG & TAI protocol in replacement beef heifers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 375-376
Author(s):  
Nicky Oosthuizen ◽  
Gabriela Melo ◽  
Lawton Stewart ◽  
George Seidel ◽  
Graham Cliff Lamb ◽  
...  
Keyword(s):  
Prostaglandin F2α ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Transrectal Ultrasonography ◽  
Dominant Follicle ◽  
Beef Heifers ◽  
Treatment Groups ◽  
Follicle Diameter ◽  
Randomized Design ◽  
Angus Heifers

Abstract To determine the effects of delaying the injection of prostaglandin F2α (PGF) and fixed-time artificial insemination (TAI) in the 14-d CIDR-PG & TAI protocol, 911 Angus heifers at 5 locations were enrolled in a completely randomized design. Within location heifers were randomly assigned to 1 of 2 treatment groups: 1) PG16 (n = 452), heifers received a CIDR insert on d 0 for 14 d, a 25-mg injection of PGF 16 d after CIDR removal [d 30], and a 100-µg injection of gonadotropin-releasing hormone concurrently with TAI 66 ± 2 h later; or 2) PG17 (n = 459), heifers were treated the same as PG16, however, PGF was administered 17 d after CIDR removal [d 31], and heifers were TAI 66 ± 2 h later. Estrus detection patches were applied to heifers at the time of PGF administration and were examined for activation at TAI. Dominant follicle diameter was determined via transrectal ultrasonography at PGF administration and TAI in a subset of heifers (n = 171). Furthermore, transrectal ultrasonography was performed to determine pregnancy rates to TAI (PR/AI) between 30 and 45 d after TAI. Estrus expression prior to TAI was similar (P = 0.50) between treatments (48.9 vs. 52.0%, respectively). Moreover, dominant follicle diameter at PGF and TAI was similar (P ≥ 0.43) between PG16 and PG17 heifers. Pregnancy rates to TAI did not differ (P = 0.48) between treatment groups (46.4 vs. 48.9%, respectively). The results of this experiment indicate that delaying the injection of PGF from d 30 to d 31 along with TAI in the 14-day CIDR-PG & TAI protocol had no effects on fertility parameters in beef heifers. In conclusion, the PGF injection and TAI in the 14-d CIDR-PG & TAI protocol may be delayed, providing more flexibility in scheduling without negatively affecting fertility.

233 Estrus Response and Pregnancy Rates of Beef Replacement Heifers Enrolled in Two Fixed-time Artificial Insemination Protocols, with or Without Pre-synchronization

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 125-126
Author(s):  
Vitor R G Rodrigues Gomes Mercadante ◽  
G Cliff C Lamb ◽  
Nicky Oosthuizen ◽  
Nicholas W Wege Dias ◽  
Stefania Pancini ◽  
...  
Keyword(s):  
Body Weight ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Prolonged Exposure ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Transrectal Ultrasonography ◽  
Beef Heifers ◽  
Estrus Response ◽  
Exogenous Progesterone

Abstract Pre-synchronization strategies with prolonged exposure to exogenous progesterone have the potential to alter estrus expression and improve fertility in replacement beef heifers. The objective of this study was to evaluate the estrus response and pregnancy rate of beef heifers enrolled in two fixed-time artificial insemination (TAI) protocols. A total of 306 heifers (14 ± 2 months of age; 372 ± 24 kg body weight [BW]) at three locations were enrolled in the study. Within location, heifers were stratified by age and BW and randomly assigned to one of two treatments: 1) PG-CIDR54 (n = 152), heifers were administered prostaglandin F2α (PGF; 25 mg) and a controlled internal drug releasing (CIDR) insert (Day -14), an injection of GnRH [100 μg (Day -7)], PGF injection and CIDR removal (Day 0), and a second injection of GnRH concurrently with TAI 54 ± 2 h later (Day 2); 2) 7-day CO-Synch+CIDR (CTRL; n = 154), heifers were administered GnRH (100 μg) and a CIDR insert (Day -7), PGF (25 mg) injection and CIDR removal (Day 0), and a second injection of GnRH concurrently with TAI 54 ± 2 h later (Day 2). All heifers received an estrus alert patch on Day 0 and were evaluated for patch activation at TAI on Day 2. Pregnancy was diagnosed by transrectal ultrasonography between 35 and 55 days after TAI. The percentage of heifers exhibiting estrus between Day 0 and 2 was greater (P &lt; 0.01) in PG-CIDR54 compared with CTRL (77.0 and 39.6%, respectively). In addition, pregnancy rate to TAI tended (P = 0.08) to be greater for PG-CIDR54 compared with CTRL (48.0 and 38.3%, respectively). We conclude that beef heifers enrolled in the PG-CIDR54 protocol had increased estrus expression and tended to have greater pregnancy rate when compared with heifers enrolled in the 7-day CO-Synch+CIDR protocol.

scholarly journals PSV-18 The role of leptin in feed efficiency and behavior attributes of commercial beef heifers

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 162-162
Author(s):  
Nayan Bhowmik ◽  
Kris A Ringwall ◽  
Carl R Dahlen ◽  
Kendall C Swanson ◽  
Jeffrey A Clapper ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Breeding Season ◽  
Feed Intake ◽  
Feed Efficiency ◽  
Repeated Measures ◽  
Fixed Effects ◽  
Beef Heifers ◽  
Frame Size ◽  
And Behavior ◽  
Plasma Leptin

Abstract Leptin is a 16-kDa-peptide hormone product of the leptin gene (LEP) that is predominantly synthesized by adipose tissues and has known involvement in regulation of feed intake, energy expenditure, reproduction, and immune functions. The objective was to determine the association of leptin genotype (LEP c.73C &gt;T), leptin diplotype (LEPD) and plasma leptin hormone (LEPH) concentration with growth, feed intake, feed efficiency, and behavior characteristics in developing beef heifers. A total of 336 commercial beef heifers were genotyped for the LEP c.73C &gt;T marker. Four single nucleotide polymorphism markers including LEP c.73C &gt;T, ARS-BFGL-NGS-59298 (intron 1), BovineHD0400026029 (upstream) and BovineHD0400026063 (downstream) of LEP were considered for haplotype analysis. Eleven LEPD were identified for this study using parsimony-based analyses. Circulating levels of LEPH were measured on 333 heifers prior to their first breeding season and used to assign low or high concentration group based on population median. Data were analyzed using the mixed procedure of SAS for repeated measures, fixed effects of ancestral breed group (n = 4), dam age category (n = 4), frame size group (n = 4), project cycle nested within birth year (n = 6), week of feed trial × year, LEP (n = 3) or LEPD (n = 11) or LEPH (n = 2), as well as week × LEPH (only for LEPH). There were no significant differences between LEP genotypes (P ≥ 0.11) or LEPD (P ≥ 0.33) for any of the studied traits. Heifers with low LEPH ate fewer meals per day (P = 0.02) and had more DMI per meal (P = 0.04) compared to those with high plasma leptin. Further research is needed to better understand plasma LEPH concentration and its role in feeding behavior attributes. However, circulatory LEPH before the breeding season may serve as a predictor for feeding behavior.

Seasonal and steroid-dependent effects on the modulation of LH secretion in the ewe by intracerebroventricularly administered β-endorphin or naloxone

1989 ◽  
Vol 122 (2) ◽  
pp. 509-517 ◽  
Author(s):  
R. J. E. Horton ◽  
H. Francis ◽  
I. J. Clarke
Keyword(s):  
Breeding Season ◽  
Luteal Phase ◽  
Pulse Frequency ◽  
Opioid Peptide ◽  
Follicular Phase ◽  
Oestrous Cycle ◽  
Opioid Antagonist ◽  
Endogenous Opioid ◽  
Endogenous Opioid Peptide ◽  
Lh Secretion

ABSTRACT The natural opioid ligand, β-endorphin, and the opioid antagonist, naloxone, were administered intracerebroventricularly (i.c.v.) to evaluate effects on LH secretion in ovariectomized ewes and in ovariectomized ewes treated with oestradiol-17β plus progesterone either during the breeding season or the anoestrous season. Ovary-intact ewes were also studied during the follicular phase of the oestrous cycle. Jugular blood samples were taken at 10-min intervals for 8 h and either saline (20–50 μl), 100 μg naloxone or 10 μg β-endorphin were injected i.c.v. after 4 h. In addition, luteal phase ewes were injected i.c.v. with 25 μg β-endorphin(1–27), a purported endogenous opioid antagonist. In ovariectomized ewes, irrespective of season, saline and naloxone did not affect LH secretion, but β-endorphin decreased the plasma LH concentrations, by reducing LH pulse frequency. The effect of β-endorphin was blocked by administering naloxone 30 min beforehand. Treating ovariectomized ewes with oestradiol-17β plus progesterone during the breeding season reduced plasma LH concentrations from 6–8 μg/l to less than 1 μg/l. In these ewes, saline did not alter LH secretion, but naloxone increased LH pulse frequency and the plasma concentrations of LH within 15–20 min. During anoestrus, the combination of oestradiol-17β plus progesterone to ovariectomized ewes reduced the plasma LH concentrations from 3–5 μg/l to undetectable levels, and neither saline nor naloxone affected LH secretion. During the follicular phase of the oestrous cycle, naloxone enhanced LH pulse frequency, which resulted in increased plasma LH concentrations; saline had no effect. In these sheep, β-endorphin decreased LH pulse frequency and the mean concentrations of LH, and this effect was prevented by the previous administration of naloxone. The i.c.v. administration of β-endorphin(1–27) to luteal phase ewes did not affect LH secretion. These data demonstrate the ability of a naturally occurring opioid peptide to inhibit LH secretion in ewes during the breeding and non-breeding seasons, irrespective of the gonadal steroid background. In contrast, whilst the gonadal steroids suppress LH secretion in ovariectomized ewes during both seasons, they only appear to activate endogenous opioid peptide (EOP)-mediated inhibition of LH secretion during the breeding season. Furthermore, these data support the notion that LH secretion in ovariectomized ewes is not normally under the control of EOP, so that naloxone has no effect. Journal of Endocrinology (1989) 122, 509–517

scholarly journals Effect of Diet on Replacement Beef Heifer Growth and Reproductive Performance

2017 ◽  
Vol 5 (2) ◽  
pp. 26
Author(s):  
Janice G. P. Bowman ◽  
Bok F. Sowell
Keyword(s):  
Reproductive Performance ◽  
Average Daily Gain ◽  
Average Weight ◽  
Beef Heifers ◽  
Roller Mill ◽  
Feed Cost ◽  
Alfalfa Hay ◽  
Grass Hay ◽  
Beef Heifer ◽  
Daily Gain

One hundred-one crossbred beef heifers (average weight 256.1 kg) were used to evaluate the effects of diet during an 84-day period, on growth and reproductive performance. The four treatments were: 1) grass hay; 2) grass hay + 0.9 kg/day barley; 3) grass hay + 1.8 kg/day barley; and 4) alfalfa hay. Heifers were assigned randomly to 12 pens by weight (three pens/treatment). Prior to feeding, barley was cracked through a roller-mill. Heifers consuming alfalfa hay or grass hay + 1.8 kg barley gained 39% faster (0.68 and 0.74 kg/day, respectively; P = 0.01) than heifers consuming grass hay alone (0.51 kg/day). Heifers consuming grass hay + 0.9 kg barley were intermediate in average daily gain (ADG; 0.63 kg/day). Hay intake was 16% greater (P < 0.10) by heifers consuming alfalfa hay, grass hay, and grass hay + 0.9 kg barley compared with those consuming grass hay + 1.8 kg barley (average 8.1 vs 7.0 kg/day). Using costs (USD) of $0.066/kg, $0.099/kg, and $0.0935/kg for grass hay, alfalfa hay, and barley, respectively, feed cost/day was greatest (P < 0.01) for alfalfa hay ($0.81/day), intermediate for the grass hay + barley diets (average $0.63/day), and lowest for grass hay ($0.53/day). Cost/kg gain was less (P < 0.10) for grass hay + 1.8 kg barley compared with alfalfa hay. No diet differences (P > 0.70) were found in reproductive performance of the heifers. Feeding replacement heifers alfalfa hay resulted in performance equivalent to feeding grass hay + 1.8 kg barley, but the alfalfa hay diet had a higher cost.

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