scholarly journals Brassinosteroid signaling integrates multiple pathways to release apical dominance in tomato

2021 ◽  
Vol 118 (11) ◽  
pp. e2004384118
Author(s):  
Xiaojian Xia ◽  
Han Dong ◽  
Yanling Yin ◽  
Xuewei Song ◽  
Xiaohua Gu ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Apical Dominance ◽  
Plant Architecture ◽  
Response Regulator ◽  
Axillary Buds ◽  
Deficient Mutant ◽  
Type B ◽  
Apical Bud ◽  
Brassinosteroid Signaling ◽  
Bud Removal

The control of apical dominance involves auxin, strigolactones (SLs), cytokinins (CKs), and sugars, but the mechanistic controls of this regulatory network are not fully understood. Here, we show that brassinosteroid (BR) promotes bud outgrowth in tomato through the direct transcriptional regulation of BRANCHED1 (BRC1) by the BR signaling component BRASSINAZOLE-RESISTANT1 (BZR1). Attenuated responses to the removal of the apical bud, the inhibition of auxin, SLs or gibberellin synthesis, or treatment with CK and sucrose, were observed in bud outgrowth and the levels of BRC1 transcripts in the BR-deficient or bzr1 mutants. Furthermore, the accumulation of BR and the dephosphorylated form of BZR1 were increased by apical bud removal, inhibition of auxin, and SLs synthesis or treatment with CK and sucrose. These responses were decreased in the DELLA-deficient mutant. In addition, CK accumulation was inhibited by auxin and SLs, and decreased in the DELLA-deficient mutant, but it was increased in response to sucrose treatment. CK promoted BR synthesis in axillary buds through the action of the type-B response regulator, RR10. Our results demonstrate that BR signaling integrates multiple pathways that control shoot branching. Local BR signaling in axillary buds is therefore a potential target for shaping plant architecture.

ZmCLA4 regulates leaf angle through multiple plant hormone-mediated signal pathways in maize

2020 ◽  
Author(s):  
Dandan Dou ◽  
Shengbo Han ◽  
Lixia Ku ◽  
Huafeng Liu ◽  
Huihui Su ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Plant Architecture ◽  
Plant Hormone ◽  
Auxin Transport ◽  
Regulatory Mechanism ◽  
Affinity Purification ◽  
Leaf Angle ◽  
Signal Pathways ◽  
Brassinosteroid Signaling ◽  
Ear Motif

AbstractLeaf angle in cereals is an important agronomic trait contributing to plant architecture and grain yield by determining the plant compactness. Although ZmCLA4 was identified to shape plant architecture by affecting leaf angle, the detailed regulatory mechanism of ZmCLA4 in maize remains unclear. ZmCLA4 was identified as a transcriptional repressor using the Gal4-LexA/UAS system and transactivation analysis in yeast. The DNA affinity purification (DAP)-seq assay showed that ZmCLA4 not only acts as a repressor containing the EAR motif (CACCGGAC), but was also found to have two new motifs, CCGARGS and CDTCNTC. On analyzing the ZmCLA4-bound targeted genes, we found that ZmCLA4, as a cross node of multiple plant hormone-mediated pathways, directly bound to ARF22 and IAA26 to regulate auxin transport and mediated brassinosteroid signaling by directly binding to BZR3 and 14-3-3. ZmCLA4 bound two WRKY genes involved with abscisic acid, two genes (CYP75B1, CYP93D1) involved with jasmonic acid, B3 involved in the response to ethylene, and thereby negatively regulated leaf angle formation. We built a new regulatory network for the ZmCLA4 gene controlling leaf angle in maize, which contributed to the understanding of ZmCLA4’s regulatory mechanism and will improve grain yields by facilitating optimization of plant architecture.

scholarly journals Release of Apical Dominance in Potato Tuber Is Accompanied by Programmed Cell Death in the Apical Bud Meristem

2012 ◽  
Vol 158 (4) ◽  
pp. 2053-2067 ◽  
Author(s):  
Paula Teper-Bamnolker ◽  
Yossi Buskila ◽  
Yael Lopesco ◽  
Shifra Ben-Dor ◽  
Inbal Saad ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Potato Tuber ◽  
Apical Dominance ◽  
Apical Bud

scholarly journals The Relationship between AGAMOUS and Cytokinin Signaling in the Establishment of Carpeloid Features

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 827
Author(s):  
Andrea Gómez-Felipe ◽  
Daniel Kierzkowski ◽  
Stefan de Folter
Keyword(s):  
Regulatory Network ◽  
Dependent Manner ◽  
Mads Box ◽  
Type B ◽  
Cytokinin Signaling ◽  
Reproductive Structures ◽  
Carpel Development ◽  
Gene Regulatory ◽  
The Relationship ◽  
Gynoecium Development

Gynoecium development is dependent on gene regulation and hormonal pathway interactions. The phytohormones auxin and cytokinin are involved in many developmental programs, where cytokinin is normally important for cell division and meristem activity, while auxin induces cell differentiation and organ initiation in the shoot. The MADS-box transcription factor AGAMOUS (AG) is important for the development of the reproductive structures of the flower. Here, we focus on the relationship between AG and cytokinin in Arabidopsis thaliana, and use the weak ag-12 and the strong ag-1 allele. We found that cytokinin induces carpeloid features in an AG-dependent manner and the expression of the transcription factors CRC, SHP2, and SPT that are involved in carpel development. AG is important for gynoecium development, and contributes to regulating, or else directly regulates CRC, SHP2, and SPT. All four genes respond to either reduced or induced cytokinin signaling and have the potential to be regulated by cytokinin via the type-B ARR proteins. We generated a model of a gene regulatory network, where cytokinin signaling is mainly upstream and in parallel with AG activity.

The single-domain response regulator LerC functions as a connector protein in theLegionella pneumophilaeffectors regulatory network

2018 ◽  
Vol 110 (5) ◽  
pp. 741-760 ◽  
Author(s):  
Yaron S. Feldheim ◽  
Tal Zusman ◽  
Anya Kapach ◽  
Gil Segal
Keyword(s):  
Regulatory Network ◽  
Response Regulator ◽  
Single Domain

scholarly journals The Type-B Cytokinin Response Regulator ARR1 Inhibits Shoot Regeneration in an ARR12-Dependent Manner in Arabidopsis

2020 ◽  
Vol 32 (7) ◽  
pp. 2271-2291 ◽  
Author(s):  
Zhenhua Liu ◽  
Xuehuan Dai ◽  
Juan Li ◽  
Na Liu ◽  
Xiangzheng Liu ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Response Regulator ◽  
Dependent Manner ◽  
Type B ◽  
Cytokinin Response

scholarly journals Abiotic stress upregulated TaZFP34 represses the expression of type-B response regulator and SHY2 genes and enhances root to shoot ratio in wheat

Plant Science ◽  
2016 ◽  
Vol 252 ◽  
pp. 88-102 ◽  
Author(s):  
Hongping Chang ◽  
Dandan Chen ◽  
Jason Kam ◽  
Terese Richardson ◽  
Janneke Drenth ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Response Regulator ◽  
Type B ◽  
Shoot Ratio ◽  
Root To Shoot Ratio

scholarly journals Manipulating osa-MIR156f Expression by D18 Promoter to Regulate Plant Architecture and Yield Traits both in Seasonal and Ratooning Rice

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Qing Liu ◽  
Yi Su ◽  
Yunhua Zhu ◽  
Keqin Peng ◽  
Bin Hong ◽  
...  
Keyword(s):  
Plant Architecture ◽  
Transgenic Line ◽  
Axillary Buds ◽  
Yield Potential ◽  
Yield Traits ◽  
Yield Increase ◽  
Basal Node ◽  
Novel Strategy ◽  
Rice Genetic ◽  
Economical Alternative

Abstract Background Rice (Oryza sativa L.) feeds more than half of the world’s population. Ratooning rice is an economical alternative to the second seasonal rice, thus increasing the yield of ratooning rice is highly important. Results Here we report an applicable transgenic line constructed through the manipulation of osa-MIR156f expression in rice shoot using the OsGA3ox2 (D18) promoter. In seasonal rice, the D18–11 transgenic line showed moderate height and more effective tillers with normal panicle. In ratooning rice, axillary buds outgrew from the basal node of the D18–11 transgenic line before the harvest of seasonal rice. More effective tillers produced by the outgrowth of axillary buds contributed to the plant architecture improvement and yield increase. Additionally, it was found that osa-miR156f down-regulated the expression of tillering regulators, such as TEOSINTE BRANCHED1 (TB1) and LAX PANICLE 1 (LAX1). The expression of DWARF10, DWARF27 and DWARF53, three genes being involved in the biosynthesis and signaling of strigolactone (SL), decreased in the stem of the D18–11 transgenic line. Conclusion Our results indicated that the manipulation of osa-MIR156f expression may have application significance in rice genetic breeding. This study developed a novel strategy to regulate plant architecture and grain yield potential both in the seasonal and ratooning rice.

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