Transcriptome Analysis of Active Axillary Buds from Narrow-crown and Broad-crown Poplars Provides Insight into the Phytohormone Regulatory Network for Branching Angle

2021 ◽  
Author(s):  
Yanting Tian ◽  
Jinnan Wang ◽  
Haoping Guo ◽  
Kai Qu ◽  
Dong Xu ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Regulatory Network ◽  
Axillary Buds ◽  
Branching Angle ◽  
Insight Into

scholarly journals Brassinosteroid signaling integrates multiple pathways to release apical dominance in tomato

2021 ◽  
Vol 118 (11) ◽  
pp. e2004384118
Author(s):  
Xiaojian Xia ◽  
Han Dong ◽  
Yanling Yin ◽  
Xuewei Song ◽  
Xiaohua Gu ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Apical Dominance ◽  
Plant Architecture ◽  
Response Regulator ◽  
Axillary Buds ◽  
Deficient Mutant ◽  
Type B ◽  
Apical Bud ◽  
Brassinosteroid Signaling ◽  
Bud Removal

The control of apical dominance involves auxin, strigolactones (SLs), cytokinins (CKs), and sugars, but the mechanistic controls of this regulatory network are not fully understood. Here, we show that brassinosteroid (BR) promotes bud outgrowth in tomato through the direct transcriptional regulation of BRANCHED1 (BRC1) by the BR signaling component BRASSINAZOLE-RESISTANT1 (BZR1). Attenuated responses to the removal of the apical bud, the inhibition of auxin, SLs or gibberellin synthesis, or treatment with CK and sucrose, were observed in bud outgrowth and the levels of BRC1 transcripts in the BR-deficient or bzr1 mutants. Furthermore, the accumulation of BR and the dephosphorylated form of BZR1 were increased by apical bud removal, inhibition of auxin, and SLs synthesis or treatment with CK and sucrose. These responses were decreased in the DELLA-deficient mutant. In addition, CK accumulation was inhibited by auxin and SLs, and decreased in the DELLA-deficient mutant, but it was increased in response to sucrose treatment. CK promoted BR synthesis in axillary buds through the action of the type-B response regulator, RR10. Our results demonstrate that BR signaling integrates multiple pathways that control shoot branching. Local BR signaling in axillary buds is therefore a potential target for shaping plant architecture.

MYB44 competitively inhibits the formation of the MYB340-bHLH2-NAC56 complex to regulate anthocyanin biosynthesis in purple-fleshed sweet potato

2020 ◽  
Author(s):  
Zeng-Zheng Wei ◽  
Kang-Di Hu ◽  
Dong-Lan Zhao ◽  
Jun Tang ◽  
Zhong-Qin Huang ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Regulatory Network ◽  
Anthocyanin Biosynthesis ◽  
Expression System ◽  
Firefly Luciferase ◽  
Anthocyanin Accumulation ◽  
Tuberous Roots ◽  
Regulatory Complex ◽  
Transcriptional Complex ◽  
Insight Into

Abstract Background: Anthocyanins, which have important biological functions and have a beneficial effect on human health, notably account for pigmentation in purple-fleshed sweet potato tuberous roots. Individual regulatory factors of anthocyanin biosynthesis have been identified; however, the regulatory network of anthocyanin biosynthesis in purple-fleshed sweet potato is unclear. Results: We functionally determined that IbMYB340 cotransformed with IbbHLH2 in tobacco and strawberry receptacles induced anthocyanin accumulation, and the addition of IbNAC56a or IbNAC56b caused increased pigmentation. Furthermore, we confirmed the interaction of IbMYB340 with IbbHLH2 and IbNAC56a or IbNAC56b via yeast two-hybrid and firefly luciferase complementation assays; these proteins could form a MYB340-bHLH2-NAC56a or MYB340-bHLH2-NAC56b transcriptional complex to regulate anthocyanin biosynthesis by binding to the IbANS promoter rather than the IbUFGT promoter. Furthermore, it was found by a transient expression system in tobacco leaves that IbMYB44 could decrease anthocyanin accumulation. Moreover, the interaction of IbMYB44 with IbMYB340 and IbNAC56a or IbNAC56b was verified. This result suggested that IbMYB44 acts as a repressor of anthocyanin in sweet potato.Conclusions: The repressor IbMYB44 affected anthocyanin biosynthesis by competitively inhibiting the IbMYB340-IbbHLH2-IbNAC56a or IbMYB340-IbbHLH2-IbNAC56b regulatory complex formation. Overall, the present study proposed a novel regulatory network whereby several vital TFs play key roles in regulating anthocyanin biosynthesis, and it provides strong insight into the potential mechanism underlying anthocyanin biosynthesis in sweet potato tuberous roots with purple color.

scholarly journals Transcriptome analysis reveals insight into molecular hydrogen-induced cadmium tolerance in alfalfa: the prominent role of sulfur and (homo)glutathione metabolism

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Weiti Cui ◽  
Ping Yao ◽  
Jincheng Pan ◽  
Chen Dai ◽  
Hong Cao ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Molecular Hydrogen ◽  
Glutathione Metabolism ◽  
Cadmium Tolerance ◽  
Insight Into

scholarly journals Transcriptome Analysis and Identification of a Transcriptional Regulatory Network in the Response to H2O2

2019 ◽  
Vol 180 (3) ◽  
pp. 1629-1646 ◽  
Author(s):  
Ayaka Hieno ◽  
Hushna Ara Naznin ◽  
Keiko Inaba-Hasegawa ◽  
Tomoko Yokogawa ◽  
Natsuki Hayami ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Regulatory Network ◽  
Transcriptional Regulatory Network ◽  
Transcriptional Regulatory

scholarly journals Unique MicroRNA and mRNA Interactions in EGFR-Mutated Lung Adenocarcinoma

2018 ◽  
Vol 7 (11) ◽  
pp. 419 ◽  
Author(s):  
Sophia Subat ◽  
Kentaro Inamura ◽  
Hironori Ninomiya ◽  
Hiroko Nagano ◽  
Sakae Okumura ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Mrna Expression ◽  
Regulatory Network ◽  
Egfr Mutation ◽  
Expression Profiles ◽  
Integrative Analysis ◽  
Future Research ◽  
Mutation Status ◽  
Egfr Mutated ◽  
Insight Into

The EGFR gene was one of the first molecules to be selected for targeted gene therapy. EGFR-mutated lung adenocarcinoma, which is responsive to EGFR inhibitors, is characterized by a distinct oncogenic pathway in which unique microRNA (miRNA)–mRNA interactions have been observed. However, little information is available about the miRNA–mRNA regulatory network involved. Both miRNA and mRNA expression profiles were investigated using microarrays in 155 surgically resected specimens of lung adenocarcinoma with a known EGFR mutation status (52 mutated and 103 wild-type cases). An integrative analysis of the data was performed to identify the unique miRNA–mRNA regulatory network in EGFR-mutated lung adenocarcinoma. Expression profiling of miRNAs and mRNAs yielded characteristic miRNA/mRNA signatures (19 miRNAs/431 mRNAs) in EGFR-mutated lung adenocarcinoma. Five of the 19 miRNAs were previously listed as EGFR-mutation-specific miRNAs (i.e., miR-532-3p, miR-500a-3p, miR-224-5p, miR-502-3p, and miR-532-5p). An integrative analysis of miRNA and mRNA expression revealed a refined list of putative miRNA–mRNA interactions, of which 63 were potentially involved in EGFR-mutated tumors. Network structural analysis provided a comprehensive view of the complex miRNA–mRNA interactions in EGFR-mutated lung adenocarcinoma, including DUSP4 and MUC4 axes. Overall, this observational study provides insight into the unique miRNA–mRNA regulatory network present in EGFR-mutated tumors. Our findings, if validated, would inform future research examining the interplay of miRNAs and mRNAs in EGFR-mutated lung adenocarcinoma.

Insight into Catechins Metabolic Pathways of Camellia sinensis Based on Genome and Transcriptome Analysis

2018 ◽  
Vol 66 (16) ◽  
pp. 4281-4293 ◽  
Author(s):  
Wenzhao Wang ◽  
Yihui Zhou ◽  
Yingling Wu ◽  
Xinlong Dai ◽  
Yajun Liu ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Transcriptome Analysis ◽  
Metabolic Pathways ◽  
Insight Into
Sign in / Sign up

Export Citation Format

Share Document