ARF small GTPases in the developmental function mediated by ARF regulators GNOM and VAN3

ARF small GTPases are molecular switches acting in intracellular trafficking. Their cycles of activity are controlled by regulators, ARF Guanine nucleotide Exchange Factors (ARF-GEFs) and ARF GTPase Activating Proteins (ARF-GAPs). The ARF-GEF GNOM (GN) and the ARF-GAP VAN3 share a prominent function in auxin-mediated developmental patterning, but the ARFs which they might control were not identified. We conducted a loss-of-function and localization-based screening of the ARF/ARF-LIKE gene family in Arabidopsis thaliana with the primary aim of identifying functional partners of GN and VAN3, while extending the limited understanding of this gene group as a whole. We identified a function of ARLA1 in branching angle control. Mutants lacking the variably localized ARLB1, ARFB1, ARFC1, ARFD1, and ARF3, even in high order combinations, do not exhibit any evident phenotypes. Loss of function arfa1 phenotypes support a major role of ARFA1 in growth and development overall, but patterning defects typical to gn loss of function are not found. ARFA1 are not localized at the plasma membrane, where GN and VAN3 carry out developmental patterning function according to current models. Taken together, putative ARF partners of GN and VAN3 in developmental patterning cannot be conclusively identified.

Investigation of Root Morphological Traits Using 2D-Imaging among Diverse Soybeans (Glycine max L.)

Roots are the most important plant organ for absorbing essential elements, such as water and nutrients for living. To develop new climate-resilient soybean cultivars, it is essential to know the variation in root morphological traits (RMT) among diverse soybean for selecting superior root attribute genotypes. However, information on root morphological characteristics is poorly understood due to difficulty in root data collection and visualization. Thus, to overcome this problem in root research, we used a 2-dimensional (2D) root image in identifying RMT among diverse soybeans in this research. We assessed RMT in the vegetative growth stage (V2) of 372 soybean cultivars propagated in polyvinyl chloride pipes. The phenotypic investigation revealed significant variability among the 372 soybean cultivars for RMT. In particular, RMT such as the average diameter (AD), surface area (SA), link average length (LAL), and link average diameter (LAD) showed significant variability. On the contrary RMT, as with total length (TL) and link average branching angle (LABA), did not show differences. Furthermore, in the distribution analysis, normal distribution was observed for all RMT; at the same time, difference was observed in the distribution curve depending on individual RMT. Thus, based on overall RMT analysis values, the top 5% and bottom 5% ranked genotypes were selected. Furthermore, genotypes that showed most consistent for overall RMT have ranked accordingly. This ultimately helps to identify four genotypes (IT 16538, IT 199127, IT 165432, IT 165282) ranked in the highest 5%, whereas nine genotypes (IT 23305, IT 208266, IT 165208, IT 156289, IT 165405, IT 165019, IT 165839, IT 203565, IT 181034) ranked in the lowest 5% for RMT. Moreover, principal component analysis clustered cultivar 2, cultivar 160, and cultivar 274 into one group with high RMT values, and cultivar 335, cultivar 40, and cultivar 249 with low RMT values. The RMT correlation results revealed significantly positive TL and AD correlations with SA (r = 0.96) and LAD (r = 0.85), respectively. However, negative correlations (r = −0.43) were observed between TL and AD. Similarly, AD showed a negative correlation (r = −0.22) with SA. Thus, this result suggests that TL is a more vital factor than AD for determining SA compositions.

Comparitive Study of the Geomorphological Characteristics of Valley Networks between Mars and the Qaidam Basin

The complex valley networks that cross the Martian surface offer geomorphologic evidence of the presence of liquid water at some point in its history. However, the derivation of both temporal and hydrological dimensions of this climate phase is far from settled. Studies comparing terrestrial fluvial networks of known formation environments with those on Mars can be used as a key to unlock the past. This work represents an analogy study and comparison between the river networks in the Qaidam Basin and those on Mars. As the Martian valley networks formed in different geologic periods with characteristic and unique features, three cases from the Noachian to the Amazonian were selected to be compared with streams in the Mangya area, where the climate is extremely arid. In terms of the maturity of the dendritic river system, shape, concave index, and branching angle (BA), the valley network in the Mangya area is comparable to Naktong Vallis, dated to the Hesperian. We also calculated throughout the valley networks on Mars the parameters of the BA and the concave index, both of which are important climatic indicators. The results show that the climate on Mars became progressively more arid, starting from the Noachian up to the Amazonian.

Retinal microvasculature and time to pregnancy in a multi-ethnic pre-conception cohort in Singapore

STUDY QUESTION Can abnormalities in retinal microvasculature representing adverse microcirculatory perfusion and inflammation shed light on the pathophysiology of female fecundability? SUMMARY ANSWER In our prospective study, abnormalities in retinal vascular geometric morphology (i.e. sparser arteriolar fractal and larger venular bifurcation) during pre-conception phase are temporarily associated with a prolonged time-to-pregnancy (TTP). WHAT IS KNOWN ALREADY Suboptimal retinal microcirculatory morphology has been associated with obesity, psychological stress and hypertension, all of which are known risk factors for reduced female fecundability. STUDY DESIGN, SIZE, DURATION A total of 652 women of Chinese, Malay or Indian ethnicity 18–45 years of age and planning to conceive spontaneously within the next 12 months were recruited during the pre-conception period into the Singapore PREconception Study of long-Term maternal and child Outcomes (S-PRESTO), from February 2015 to October 2017. PARTICIPANTS/MATERIALS, SETTING, METHODS During recruitment, we collected information on socio-demographic factors, menstrual characteristics and lifestyle behaviors and made anthropometric measurements. We assessed the following retinal microvascular features: caliber, branching angle and fractal dimension. We conducted follow-up telephone surveys to track each participant’s pregnancy status at 6, 9 and 12 months after enrolment. We ascertained clinical pregnancies via ultrasonography, with TTP measured by the number of menstrual cycles required to achieve a clinical pregnancy over a 1-year follow-up. Then, we performed discrete-time proportional hazards models to estimate the fecundability odds ratio (FOR) and 95% CI for each retinal microvascular feature in association with TTP, after adjusting for major confounders, including body mass index and fasting glycemic level at study entry. MAIN RESULTS AND THE ROLE OF THE CHANCE Among 652 recruited women, 276 (42.3%) successfully conceived within 1 year of follow-up. The mean (and SD) was 1.24 (0.05) Df for retinal arteriolar dimension fraction and 78.45 (9.79) degrees for retinal venular branching angle, respectively. Non-linear relationship testing was performed before multiple adjustment in all associations and a non-monotonic association was detected between retinal venular branching angle and TTP. Compared with women in the highest tertile of retinal arteriolar fractal dimension, women in the second tertile had a prolonged TTP (FOR: 0.68; 95% CI: 0.51–0.92), as did women in the lowest tertile (FOR: 0.73; 95% CI: 0.55–0.98). Compared with women in the middle tertile of retinal venular branching angle, women in the highest tertile had a borderline prolonged TTP (FOR: 0.75; 95% CI: 0.56–1.02). No other retinal vascular features were significantly associated with TTP. LIMITATIONS, REASONS FOR CAUTION We were unable to adjust for other potential confounding factors such as female sexual function (e.g. frequency of sexual intercourse), which might introduce a residual bias. Moreover, even though this is a prospective cohort design, our findings can identify the temporal relationship but not necessarily infer a causal relationship between maternal microvasculature and TTP. Lastly, our study involving mainly Chinese, Malay and Indian ethnicities might not be generalizable to other races or ethnicities. WIDER IMPLICATIONS OF THE FINDINGS Suboptimal microcirculation may lead to reduced female fecundability. In the future, in addition to conventional ultrasonographic evaluation of ovarian and uterine physiological function, assessing the retinal microvasculature might be useful for assessment of ovarian age, fertility prediction and endometrial evaluation before assisted reproductive techniques for fertility treatments. STUDY FUNDING/COMPETING INTEREST(S) This research is supported by the Singapore National Research Foundation (NRF) under its Translational and Clinical Research (TCR) Flagship Programme and administered by the Singapore Ministry of Health’s National Medical Research Council (NMRC) (Singapore-NMRC/TCR/004-NUS/2008; NMRC/TCR/012-NUHS/2014) and Singapore National Medical Research Council Transition Award (NMRC TA/0027/2014). The authors have no competing interests to declare. TRIAL REGISTRATION NUMBER ClinicalTrials.gov, NCT03531658.

Forced Convective Flow of Bingham Plastic Fluids in a Branching Channel With the Effect of T-Channel Branching Angle

This work aims to explore the T-channel momentum and heat transfer characteristics with the combined effect of Bingham plastic fluids (0.01 ≤ Bn ≤ 20) behavior and geometrical variation in terms of branching angle (30 deg ≤ α ≤ 90 deg). The problem has been solved over a wide range of Reynolds number (50 ≤ Re ≤ 300) and Prandtl number (10 ≤ Pr ≤ 50). For the momentum flow, qualitative and quantitative features are analyzed in terms of streamlines, structure of yielded/unyielded regions, shear rate contours, plug width and length variation, and local pressure coefficient. These features have been represented in terms of isotherm patterns, temperature profile, Nusselt number, and its asymptotic value for heat transfer characteristics. The recirculating flows have been presented here in the vicinity of T-junction, which promote mixing and heat transfer. Broadly, the size of this zone bears a positive dependence on Re and α. However, fluid yield stress tends to suppress it. The critical Reynolds and Bingham numbers were found to be strong functions of the pertinent parameters like α. The inclination angle exerts only a weak effect on the yielded/unyielded regions and on the recirculation length of main branch. Results show a strong relationship of the plug width and length with key parameters and branches. The Nusselt number exhibits a positive relationship with α, Bn, and Re but for lower Pr in the T-junction vicinity for both branches. Such length indicates the required optimum channel length for thermal mixing.

Simulation of vortex characteristics in a planar ψ-shaped branching microchannel with lattice Boltzmann method

The vortex in the branching microchannel enhances the mixing and heat transfer performance. To investigate the vortex intensity quantitatively, a lattice Boltzmann model for incompressible power-law fluid is developed by setting the range of lattice viscosity (0.001 [Formula: see text] 1). The validation of the current model is carried out by modeling the vortex in a T-shaped branching channel and the Poiseuille flow of power-law fluids. Then the vortex intensity in the [Formula: see text]-shaped microchannel is numerically studied in terms of Reynolds number, branching angle and power-law index. The result indicates that both the recirculation length and height increase with the increase of the Reynolds number. The branching angle has a negative impact on the recirculation length, and it has little effect on the recirculation height. The influence of the power-law index on recirculation length and height depends on the Reynolds number.