scholarly journals Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii

2016 ◽  
Vol 291 (33) ◽  
pp. 17478-17487 ◽  
Author(s):  
Viviana Correa-Galvis ◽  
Petra Redekop ◽  
Katharine Guan ◽  
Annika Griess ◽  
Thuy B. Truong ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Chlamydomonas Reinhardtii ◽  
Conformational Changes ◽  
Light Exposure ◽  
Complex Stress ◽  
High Light ◽  
Photochemical Quenching ◽  
Light Illumination ◽  
Non Photochemical Quenching ◽  
Psbs Protein

Non-photochemical quenching of excess excitation energy is an important photoprotective mechanism in photosynthetic organisms. In Arabidopsis thaliana, a high quenching capacity is constitutively present and depends on the PsbS protein. In the green alga Chlamydomonas reinhardtii, non-photochemical quenching becomes activated upon high light acclimation and requires the accumulation of light harvesting complex stress-related (LHCSR) proteins. Expression of the PsbS protein in C. reinhardtii has not been reported yet. Here, we show that PsbS is a light-induced protein in C. reinhardtii, whose accumulation under high light is further controlled by CO2 availability. PsbS accumulated after several hours of high light illumination at low CO2. At high CO2, however, PsbS was only transiently expressed under high light and was degraded after 1 h of high light exposure. PsbS accumulation correlated with an enhanced non-photochemical quenching capacity in high light-acclimated cells grown at low CO2. However, PsbS could not compensate for the function of LHCSR in an LHCSR-deficient mutant. Knockdown of PsbS accumulation led to reduction of both non-photochemical quenching capacity and LHCSR3 accumulation. Our data suggest that PsbS is essential for the activation of non-photochemical quenching in C. reinhardtii, possibly by promoting conformational changes required for activation of LHCSR3-dependent quenching in the antenna of photosystem II.

scholarly journals The role of LHCBM1 in non-photochemical quenching in Chlamydomonas reinhardtii

2022 ◽  
Author(s):  
Xin Liu ◽  
Wojciech J Nawrocki ◽  
Roberta Croce
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Protein Complexes ◽  
Ph Sensor ◽  
High Light ◽  
Photochemical Quenching ◽  
Knock Out ◽  
Photosynthetic Organisms ◽  
Pigment Protein Complexes ◽  
Non Photochemical Quenching

Non-photochemical quenching (NPQ) is the process that protects photosynthetic organisms from photodamage by dissipating the energy absorbed in excess as heat. In the model green alga Chlamydomonas reinhardtii, NPQ was abolished in the knock-out mutants of the pigment-protein complexes LHCSR3 and LHCBM1. However, while LHCSR3 was shown to be a pH sensor and switching to a quenched conformation at low pH, the role of LHCBM1 in NPQ has not been elucidated yet. In this work, we combine biochemical and physiological measurements to study short-term high light acclimation of npq5, the mutant lacking LHCBM1. We show that while in low light in the absence of this complex, the antenna size of PSII is smaller than in its presence, this effect is marginal in high light, implying that a reduction of the antenna is not responsible for the low NPQ. We also show that the mutant expresses LHCSR3 at the WT level in high light, indicating that the absence of this complex is also not the reason. Finally, NPQ remains low in the mutant even when the pH is artificially lowered to values that can switch LHCSR3 to the quenched conformation. It is concluded that both LHCSR3 and LHCBM1 need to be present for the induction of NPQ and that LHCBM1 is the interacting partner of LHCSR3. This interaction can either enhance the quenching capacity of LHCSR3 or connect this complex with the PSII supercomplex.

scholarly journals Identification of parallel pH- and zeaxanthin-dependent quenching of excess energy in LHCSR3 in Chlamydomonas reinhardtii

2020 ◽  
Author(s):  
Julianne M. Troiano ◽  
Federico Perozeni ◽  
Raymundo Moya ◽  
Luca Zuliani ◽  
Kwangryul Baek ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Light Harvesting ◽  
Complex Stress ◽  
Excess Energy ◽  
Photochemical Quenching ◽  
Light Harvesting Complexes ◽  
Light Harvesting Complex ◽  
Non Photochemical Quenching

AbstractUnder high light conditions, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating excess absorbed energy, which is called non-photochemical quenching (NPQ). In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via pH and serves as a quenching site. However, the mechanisms by which LHCSR3 functions have not been determined. Using a combined in vivo and in vitro approach, we identify two parallel yet distinct quenching processes, individually controlled by pH and carotenoid composition, and their likely molecular origin within LHCSR3 from Chlamydomonas reinhardtii. The pH-controlled quenching is removed within a mutant LHCSR3 that lacks the protonable residues responsible for sensing pH. Constitutive quenching in zeaxanthin-enriched systems demonstrates zeaxanthin-controlled quenching, which may be shared with other light-harvesting complexes. We show that both quenching processes prevent the formation of damaging reactive oxygen species, and thus provide distinct timescales and mechanisms of protection in a changing environment.

Photosynthesis, light energy partitioning, and photoprotection in the shade-demanding species Panax notoginseng under high and low level of growth irradiance

2016 ◽  
Vol 43 (6) ◽  
pp. 479 ◽  
Author(s):  
Jun-Wen Chen ◽  
Shuang-Bian Kuang ◽  
Guang-Qiang Long ◽  
Sheng-Chao Yang ◽  
Zhen-Gui Meng ◽  
...  
Keyword(s):  
Panax Notoginseng ◽  
High Light ◽  
Light Energy ◽  
Quantum Yields ◽  
Photochemical Quenching ◽  
Light Illumination ◽  
Low Light ◽  
Psii Photochemistry ◽  
Growth Irradiance ◽  
Non Photochemical Quenching

Partitioning of light energy into several pathways and its relation to photosynthesis were examined in a shade-demanding species Panax notoginseng (Burkill) F.H.Chen ex C.Y.Wu & K.M.Feng grown along a light gradient. In fully light-induced leaves, the actual efficiency of PSII photochemistry (ΔF/Fmʹ), electron transport rate (ETR), non-photochemical quenching (NPQ) and photochemical quenching (qP) were lower in low-light-grown plants; this was also the case in fully dark-adapted leaves under a simulated sunfleck. In response to varied light intensity, high-light-grown plants showed greater quantum yields of light-dependent non-photochemical quenching (ΦNPQ) and PSII photochemistry (ΦPSII) and smaller quantum yields of fluorescence and constitutive thermal dissipation (Φf,d). Under the simulated sunfleck, high-light-grown plants showed greater ΦPSII and smaller Φf,d. There were positive relationships between net photosynthesis (Anet) and ΦNPQ+f,d and negative relationships between Anet and ΦPSII in fully light-induced leaves; negative correlations of Anet with ΦNPQ+f,d and positive correlations of Anet with ΦPSII were observed in fully dark-adapted leaves. In addition, more nitrogen was partitioned to light-harvesting components in low-light-grown plants, whereas leaf morphology and anatomy facilitate reducing light capture in high-light-grown plants. The pool of xanthophyll pigments and the de-epoxidation state was greater in high-light-grown plants. Antioxidant defence was elevated by increased growth irradiance. Overall, the evidences from P. notoginseng suggest that in high-light-grown shade-demanding plants irradiated by high light more electrons were consumed by non-net carboxylative processes that activate the component of NPQ, that low-light-grown plants correspondingly protect the photosynthetic apparatus against photodamage by reducing the efficiency of PSII photochemistry under high light illumination, and that during the photosynthetic induction, the ΔpH-dependent (qE) component of NPQ might dominate photoprotection, but the NPQ also depresses the enhancement of photosynthesis via competition for light energy.

scholarly journals Combined chlorophyll fluorescence techniques to study environmental impact on the mountain moss Polytrichum commune

2021 ◽  
Vol 11 (1) ◽  
pp. 161-173
Author(s):  
Gabriella Nora Maria Giudici
Keyword(s):  
Chlorophyll Fluorescence ◽  
Conformational Changes ◽  
Heat Dissipation ◽  
Fluorescence Induction ◽  
High Light ◽  
Chlorophyll Fluorescence Induction ◽  
Photochemical Quenching ◽  
Light Harvesting Complexes ◽  
Polytrichum Commune ◽  
Non Photochemical Quenching

Two chlorophyll fluorescence (ChlF) methods were used to study the effects of high light (photoinhibition) and dehydration, common stressors of the alpine environment, on primary photosynthetic processes in the moss Polytrichum commune from the Czech Republic, the Jeseníky Mountains. Photoinhibition (PI) was studied in fully hydrated thalli of P. commune and during the period of spontaneous desiccation. Time courses of Kautsky kinetics (KK) of ChlF and derived parameters: maximum quantum yield (FV/FM), effective quantum yeld (ΦPSII), and non-photochemical quenching parameters, were measured before and after the samples were treated with high light (1500 µmol m-2 s-1 PAR) for 60 min. Dehydration effects were tested in two sets of experiments with a Pulse-Amplitude-Modulation fluorometry (PAM) and Fast Chlorophyll Fluorescence induction curve (OJIP) techniques. In PAM tests, the desiccating samples were exposed to saturating light pulses every 10 min. in order to obtain ΦPSII and non-photochemical quenching (NPQ). In the second dehydration experiment, OJIP transients of ChlF were repeatedly recorded, OJIP-derived ChlF parameters were plotted against relative water content (RWC) monitored during desiccation. Combined ChF techniques provided insights into the mechanisms activated during P. commune desiccation, such as dissipation of excess absorbed energy through heat dissipation, and conformational changes or destructions of the light harvesting complexes. Combination of stressors resulted in amplified interference with the photosynthetic machinery, even when the added stressor (dehydration) was applied in low dose.

scholarly journals The non-photochemical quenching protein LHCSR3 prevents oxygen-dependent photoinhibition in Chlamydomonas reinhardtii

2020 ◽  
Vol 71 (9) ◽  
pp. 2650-2660 ◽  
Author(s):  
Thomas Roach ◽  
Chae Sun Na ◽  
Wolfgang Stöggl ◽  
Anja Krieger-Liszkay
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Colony Growth ◽  
High Light ◽  
Photochemical Quenching ◽  
Oxygen Species ◽  
Wild Type ◽  
Psii Activity ◽  
High O2 ◽  
Non Photochemical Quenching ◽  
Repair Cycle

Abstract Non-photochemical quenching (NPQ) helps dissipate surplus light energy, preventing formation of reactive oxygen species (ROS). In Chlamydomonas reinhardtii, the thylakoid membrane protein LHCSR3 is involved in pH-dependent (qE-type) NPQ, lacking in the npq4 mutant. Preventing PSII repair revealed that npq4 lost PSII activity faster than the wild type (WT) in elevated O2, while no difference between strains was observed in O2-depleted conditions. Low Fv/Fm values remained 1.5 h after moving cells out of high light, and this qH-type quenching was independent of LHCSR3 and not accompanied by losses of maximum PSII activity. Culturing cells in historic O2 atmospheres (30–35%) increased the qE of cells, due to increased LHCSR1 and PsbS levels, and LHCSR3 in the WT, showing that atmospheric O2 tensions regulate qE capacity. Colony growth of npq4 was severely restricted at elevated O2, and npq4 accumulated more reactive electrophile species (RES) than the WT, which could damage PSI. Levels of PsaA (PSI) were lower in npq4 grown at 35% O2, while PsbA (PSII) levels remained stable. We conclude that even at high O2 concentrations, the PSII repair cycle is sufficient to maintain net levels of PSII. However, LHCSR3 has an important function in protecting PSI against O2-mediated damage, such as via RES.

scholarly journals The carotenoid pathway: what is important for excitation quenching in plant antenna complexes?

2017 ◽  
Vol 19 (34) ◽  
pp. 22957-22968 ◽  
Author(s):  
Kieran F. Fox ◽  
Vytautas Balevičius ◽  
Jevgenij Chmeliov ◽  
Leonas Valkunas ◽  
Alexander V. Ruban ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Excitation Energy ◽  
Light Harvesting ◽  
High Light ◽  
Photochemical Quenching ◽  
Non Photochemical Quenching ◽  
Carotenoid Pathway

Plant light-harvesting is regulated by the Non-Photochemical Quenching (NPQ) mechanism involving the slow trapping of excitation energy by carotenoids in the Photosystem II (PSII) antenna in response to high light.

scholarly journals Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis

2021 ◽  
Author(s):  
Iva Ilíková ◽  
Petr Ilík ◽  
Monika Opatíková ◽  
Rameez Arshad ◽  
Lukáš Nosek ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Light Harvesting ◽  
Light Exposure ◽  
Land Plants ◽  
Photochemical Quenching ◽  
Functional Changes ◽  
Electron Microscopy Analysis ◽  
Function Characterization ◽  
Microscopy Analysis ◽  
Non Photochemical Quenching

Abstract The largest stable photosystem II (PSII) supercomplex in land plants (C2S2M2) consists of a core complex dimer (C2), two strongly (S2) and two moderately (M2) bound light-harvesting protein (LHCB) trimers attached to C2 via monomeric antenna proteins LHCB4–6. Recently, we have shown that LHCB3 and LHCB6, presumably essential for land plants, are missing in Norway spruce (Picea abies), which results in a unique structure of its C2S2M2 supercomplex. Here, we performed structure–function characterization of PSII supercomplexes in Arabidopsis (Arabidopsis thaliana) mutants lhcb3, lhcb6, and lhcb3 lhcb6 to examine the possibility of the formation of the “spruce-type” PSII supercomplex in angiosperms. Unlike in spruce, in Arabidopsis both LHCB3 and LHCB6 are necessary for stable binding of the M trimer to PSII core. The “spruce-type” PSII supercomplex was observed with low abundance only in the lhcb3 plants and its formation did not require the presence of LHCB4.3, the only LHCB4-type protein in spruce. Electron microscopy analysis of grana membranes revealed that the majority of PSII in lhcb6 and namely in lhcb3 lhcb6 mutants were arranged into C2S2 semi-crystalline arrays, some of which appeared to structurally restrict plastoquinone diffusion. Mutants without LHCB6 were characterized by fast induction of non-photochemical quenching and, on the contrary to the previous lhcb6 study, by only transient slowdown of electron transport between PSII and PSI. We hypothesize that these functional changes, associated with the arrangement of PSII into C2S2 arrays in thylakoids, may be important for the photoprotection of both PSI and PSII upon abrupt high-light exposure.

Light Absorption and Partitioning in Response to Nitrogen in Apple Leaves

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 541a-541
Author(s):  
Lailiang Cheng ◽  
Leslie H. Fuchigami ◽  
Patrick J. Breen
Keyword(s):  
High Light ◽  
Thermal Dissipation ◽  
Photochemical Quenching ◽  
Light Conditions ◽  
Psii Efficiency ◽  
Fluorescence Measurements ◽  
Free Radical Formation ◽  
Highly Correlated ◽  
Non Photochemical Quenching ◽  
Leaf N

Bench-grafted Fuji/M26 apple trees were fertigated with different concentrations of nitrogen by using a modified Hoagland solution for 6 weeks, resulting in a range of leaf N from 1.0 to 4.3 g·m–2. Over this range, leaf absorptance increased curvilinearly from 75% to 92.5%. Under high light conditions (1500 (mol·m–2·s–1), the amount of absorbed light in excess of that required to saturate CO2 assimilation decreased with increasing leaf N. Chlorophyll fluorescence measurements revealed that the maximum photosystem II (PSII) efficiency of dark-adapted leaves was relatively constant over the leaf N range except for a slight drop at the lower end. As leaf N increased, non-photochemical quenching under high light declined and there was a corresponding increase in the efficiency with which the absorbed photons were delivered to open PSII centers. Photochemical quenching coefficient decreased significantly at the lower end of the leaf N range. Actual PSII efficiency increased curvilinearly with increasing leaf N, and was highly correlated with light-saturated CO2 assimilation. The fraction of absorbed light potentially used for free radical formation was estimated to be about 10% regardless of the leaf N status. It was concluded that increased thermal dissipation protected leaves from photo-oxidation as leaf N declined.

scholarly journals Gradual Response of Cyanobacterial Thylakoids to Acute High-Light Stress—Importance of Carotenoid Accumulation

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1916
Author(s):  
Myriam Canonico ◽  
Grzegorz Konert ◽  
Aurélie Crepin ◽  
Barbora Šedivá ◽  
Radek Kaňa
Keyword(s):  
Single Cell ◽  
Thylakoid Membrane ◽  
Intermediate Phase ◽  
Light Stress ◽  
Fast Response ◽  
High Light ◽  
Photochemical Quenching ◽  
Second Phase ◽  
Ros Scavenging ◽  
Non Photochemical Quenching

Light plays an essential role in photosynthesis; however, its excess can cause damage to cellular components. Photosynthetic organisms thus developed a set of photoprotective mechanisms (e.g., non-photochemical quenching, photoinhibition) that can be studied by a classic biochemical and biophysical methods in cell suspension. Here, we combined these bulk methods with single-cell identification of microdomains in thylakoid membrane during high-light (HL) stress. We used Synechocystis sp. PCC 6803 cells with YFP tagged photosystem I. The single-cell data pointed to a three-phase response of cells to acute HL stress. We defined: (1) fast response phase (0–30 min), (2) intermediate phase (30–120 min), and (3) slow acclimation phase (120–360 min). During the first phase, cyanobacterial cells activated photoprotective mechanisms such as photoinhibition and non-photochemical quenching. Later on (during the second phase), we temporarily observed functional decoupling of phycobilisomes and sustained monomerization of photosystem II dimer. Simultaneously, cells also initiated accumulation of carotenoids, especially ɣ–carotene, the main precursor of all carotenoids. In the last phase, in addition to ɣ-carotene, we also observed accumulation of myxoxanthophyll and more even spatial distribution of photosystems and phycobilisomes between microdomains. We suggest that the overall carotenoid increase during HL stress could be involved either in the direct photoprotection (e.g., in ROS scavenging) and/or could play an additional role in maintaining optimal distribution of photosystems in thylakoid membrane to attain efficient photoprotection.

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