scholarly journals A quantitative method to detect non-antithrombin-binding 3-O-sulfated units in heparan sulfate

2020 ◽  
pp. jbc.RA120.015864
Author(s):  
Hideo Mochizuki ◽  
Hideyuki Futatsumori ◽  
Eriko Suzuki ◽  
Koji Kimata
Keyword(s):  
Heparan Sulfate ◽  
Fluorescent Labeling ◽  
P19 Cells ◽  
Reaction Products ◽  
Animal Cells ◽  
New Approach ◽  
Physiological Processes ◽  
Before And After ◽  
Compositional Changes ◽  
Labeling System

Heparan sulfate is synthesized by most animal cells and interacts with numerous proteins via specific sulfation motifs to regulate various physiological processes. Various 3-O-sulfated motifs are considered to be key in controlling the binding specificities to the functional proteins. One such motif, synthesized by 3-O-sulfotransferase-1 (3OST-1) serves as a binding site for antithrombin (AT), and has been thoroughly studied because of its pharmacological importance. However, the physiological roles of 3-O-sulfates produced by other 3OST isoforms, which do not bind AT, remain obscure, in part due to the lack of a standard method to analyze this rare modification. This study aims to establish a method for quantifying 3-O-sulfated components of heparan sulfate, focusing on non-AT-binding units. We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases. In this study, we prepared these five components as a standard saccharide for HPLC analysis. Together with eight non-3-O-sulfated disaccharides, a standard mixture of thirteen units was prepared. Using reverse-phase ion-pair HPLC with a post-column fluorescent labeling system, the separation conditions were optimized to quantify the thirteen units. Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation. We successfully detected the 3-O-sulfated units specifically expressed in the differentiated neurons. This is the first report that shows the quantification of three non-AT-binding 3-O-sulfated units, and establishes a new approach to explore the physiological functions of 3-O-sulfate.

Localization of Adenosine Triphosphatase Activity in the Phleom of Nicotiana Tabacum

1972 ◽  
Vol 30 ◽  
pp. 230-231
Author(s):  
James Cronshaw ◽  
Jamison E. Gilder
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Higher Plants ◽  
High Surface ◽  
Root Tip ◽  
Animal Cells ◽  
Physiological Processes ◽  
Tip Cells ◽  
Atpase Localization

Adenosine triphosphatase (ATPase) activity has been shown to be associated with numerous physiological processes in both plants and animal cells. Biochemical studies have shown that in higher plants ATPase activity is high in cell wall preparations and is associated with the plasma membrane, nuclei, mitochondria, chloroplasts and lysosomes. However, there have been only a few ATPase localization studies of higher plants at the electron microscope level. Poux (1967) demonstrated ATPase activity associated with most cellular organelles in the protoderm cells of Cucumis roots. Hall (1971) has demonstrated ATPase activity in root tip cells of Zea mays. There was high surface activity largely associated with the plasma membrane and plasmodesmata. ATPase activity was also demonstrated in mitochondria, dictyosomes, endoplasmic reticulum and plastids.

A new approach to treatment of stomatitis in experiments on white rats (experimental research)

2018 ◽  
pp. 168-173
Author(s):  
Е.Б. Ганина ◽  
Ю.В. Червинец ◽  
Н.В. Грудинин ◽  
В.Г. Шестакова ◽  
В.М. Червинец ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Oral Cavity ◽  
Experimental Research ◽  
Quantitative Composition ◽  
Oral Diseases ◽  
New Approach ◽  
Highly Active ◽  
White Rats ◽  
Before And After ◽  
Intact Rats

Цель исследования - охарактеризовать микробиологическую картину стоматита до и после его лечения высокоактивными культурами лактобацилл, дать оценку эффективности их применения при экспериментальном стоматите. Методика. Опыты проводились на 32 самках беспородных белых крыс массой 230 г. Моделирование стоматита включало 2 этапа: моделирование травматического стоматита и воспроизведение бактериального стоматита на базе травматического. У крыс контрольной и опытных серий на всех этапах эксперимента брали мазки с поверхности десен для характеристики микробиоценоза ротовой полости. Результаты. После обработки слизистой оболочки полости рта (СОПР) культурой патогенного штамма Staphylococcus aureus существенно снизились частота встречаемости и количество условно-патогенных микроорганизмов. Лечение стоматита у крыс культурами лактобацилл ( Lactobacillus 11 зв., Lactobacillus 2 п.рта, Lactobacillus 24 д.ст.) и их комбинацией приводило к снижению распространенности S. aureus вплоть до его исчезновения, а также к нормализации количества условно-патогенной микробиоты. Заключение. Исследования показали эффективность использования отдельных высокоактивных штаммов лактобацилл или их комбинации при лечении заболеваний СОПР, в частности бактериального стоматита. Aim. To characterize the microbiological picture of stomatitis in intact rats before and after the treatment with highly active cultured lactobacilli and to evaluate the effectiveness of this treatment in experiments on white rats. Methods. Experiments were carried out on 32 mongrel female white rats weighing 230 g. Smears were taken from the gum surface of control and experimental rats at all stages of the experiment to characterize the oral cavity microbiocenosis. Results. After treatment of the oral cavity with cultured Staphylococcus aureus , the occurrence and quantity of opportunistic microorganisms significantly decreased. The treatment of stomatitis in rats with cultured lactobacillus ( Lactobacillus 11 zv., Lactobacillus 2 p.r., Lactobacillus 24 d.st. and their combination) led to a decrease in S. aureus prevalence up to its extinction and to normalization of the quantitative composition of opportunistic microbiota. Conclusions. The study showed the effieacy of highly active lactobacillus strains individually or their combinations in the treatment of oral diseases, in particular, bacterial stomatitis.

Percentile transformation and recalibration functions allow harmonization of thyroid-stimulating hormone (TSH) immunoassay results

2020 ◽  
Vol 58 (10) ◽  
pp. 1663-1672 ◽  
Author(s):  
Andrea Padoan ◽  
Aldo Clerico ◽  
Martina Zaninotto ◽  
Tommaso Trenti ◽  
Renato Tozzoli ◽  
...  
Keyword(s):  
Robust Regression ◽  
Reference Intervals ◽  
Thyroid Stimulating Hormone ◽  
Validation Dataset ◽  
New Approach ◽  
Coefficients Of Variation ◽  
Significant Difference ◽  
Before And After ◽  
Roche Diagnostics ◽  
Stimulating Hormone

AbstractBackgroundThe comparability of thyroid-stimulating hormone (TSH) results cannot be easily obtained using SI-traceable reference measurement procedures (RPMs) or reference materials, whilst harmonization is more feasible. The aim of this study was to identify and validate a new approach for the harmonization of TSH results.MethodsPercentile normalization was applied to 125,419 TSH results, obtained from seven laboratories using three immunoassays (Access 3rd IS Thyrotropin, Beckman Coulter Diagnostics; Architect System, Abbott Diagnostics and Elecsys, Roche Diagnostics). Recalibration equations (RCAL) were derived by robust regressions using bootstrapped distribution. Two datasets, the first of 119 EQAs, the second of 610, 638 and 639 results from Access, Architect and Elecsys TSH results, respectively, were used to validate RCAL. A dataset of 142,821 TSH values was used to derive reference intervals (RIs) after applying RCAL.ResultsAccess, Abbott and Elecsys TSH distributions were significantly different (p < 0.001). RCAL intercepts and slopes were −0.003 and 0.984 for Access, 0.032 and 1.041 for Architect, −0.031 and 1.003 for Elecsys, respectively. Validation using EQAs showed that before and after RCAL, the coefficients of variation (CVs) or among-assay results decreased from 10.72% to 8.16%. The second validation dataset was used to test RCALs. The median of between-assay differences ranged from −0.0053 to 0.1955 mIU/L of TSH. Elecsys recalibrated to Access (and vice-versa) showed non-significant difference. TSH RI after RCAL resulted in 0.37–5.11 mIU/L overall, 0.49–4.96 mIU/L for females and 0.40–4.92 mIU/L for males. A significant difference across age classes was identified.ConclusionsPercentile normalization and robust regression are valuable tools for deriving RCALs and harmonizing TSH values.

scholarly journals Preparation and Electron-Beam Surface Modification of Novel TiNi Material for Medical Applications

2021 ◽  
Vol 11 (10) ◽  
pp. 4372
Author(s):  
Sergey G. Anikeev ◽  
Anastasiia V. Shabalina ◽  
Sergei A. Kulinich ◽  
Nadezhda V. Artyukhova ◽  
Daria R. Korsakova ◽  
...  
Keyword(s):  
Electron Beam ◽  
Material Surface ◽  
High Current ◽  
New Materials ◽  
New Approach ◽  
Pulsed Electron Beam ◽  
Beam Surface ◽  
Compositional Changes ◽  
Unmodified Sample ◽  
Mcf 7

A new approach to fabricate TiNi surfaces combining the advantages of both monolithic and porous materials for implants is used in this work. New materials were obtained by depositing a porous TiNi powder onto monolithic TiNi plates followed by sintering at 1200 °C. Then, further modification of the material surface with a high-current-pulsed electron beam (HCPEB) was carried out. Three materials obtained (one after sintering and two after subsequent beam treatment by 30 pulses with different pulse energy) were studied by XRD, SEM, EDX, surface profilometry, and by means of electrochemical measurements, including OCP and EIS. Structural and compositional changes caused by HCPEB treatment were investigated. Surface properties of the samples during their storage in saline for 10 days were studied and a model experiment with cell growth (MCF-7) was carried out for the unmodified sample with an electron beam to detect cell appearance on different surface locations.

Aanalysis of springback phenomenon in pipe bending

2014 ◽  
Vol 11 (3) ◽  
pp. 229-232
Author(s):  
Rahul Hingole ◽  
Vilas Nandedkar
Keyword(s):  
Sheet Metal ◽  
Research Work ◽  
Deep Understanding ◽  
Beam Theory ◽  
New Approach ◽  
Metal Parts ◽  
Sheet Metal Parts ◽  
Before And After ◽  
Forming Tools ◽  
Pipe Bending

The term springback is defined as the change in geometry of a component after forming, when the forces are removed from forming tools. As springback affects the final shape of the part, it can lead to significant difficulties in the assembly of component when springback is not proper. This problem leads to fabrication of inconsistent sheet metal parts; the elastic strain recovery in the material after the tooling is removed. Bendingis the plastic deformation of metals about a linear axis called the bending axis with little or no change in the surface area. Bending types of forming operations have been used widely in sheet metal forming industries to produce structural stamping parts such as braces, brackets, supports, hinges, angles, frames, channel and other nonsymmetrical sheet metal parts. Among them, quite a few efforts have been made to obtain a deep understanding of the springback phenomenon. The beam theory has been applied to formulate the curvature before and after loading of pipe. This research work has focused on study effect of springback effect with a new approach. The ANSYS software is used to analyze spring back effect. The detail study of this springback effect is presented in this paper.

scholarly journals Preparation and Electron-Beam Surface Modification of Novel TiNi Material for Medical Applications

2021 ◽  
Author(s):  
Sergey G. Anikeev ◽  
Anastasiia V. Shabalina ◽  
Sergei A. Kulinich ◽  
Nadezhda V. Artyukhova ◽  
Darya R. Korsakova ◽  
...  
Keyword(s):  
Electron Beam ◽  
Model Experiment ◽  
Material Surface ◽  
High Current ◽  
New Materials ◽  
New Approach ◽  
Pulsed Electron Beam ◽  
Beam Surface ◽  
Compositional Changes ◽  
Mcf 7

A new approach to fabricate TiNi surfaces combining the advantages of both monolithic and porous materials for implants is used in this work. New materials were obtained by depositing a porous TiNi powder onto monolithic TiNi plates followed by sintering at 1200°C. Then, further modification of the material surface with a high-current-pulsed electron beam (HCPEB) was carried out. Three materials obtained (one after sintering and two after subsequent beam treatment by 20 and 30 pulses, respectively) were studied by XRD, SEM, EDX, EIS methods, profilometry and OCP measurements. Structural and compositional changes caused by HCPEB treatment were investigated. Surface properties of the samples during their storage in saline for 10 days were studied and a model experiment with cell growth (MCF-7) was carried out for the sample unmodified with electron beam to detect cell appearance on different surface locations.

scholarly journals Laser Irradiation of a Bio-Waste Derived Carbon Unlocks Performance Enhancement in Secondary Lithium Batteries

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3183
Author(s):  
Mariangela Curcio ◽  
Sergio Brutti ◽  
Lorenzo Caripoti ◽  
Angela De Bonis ◽  
Roberto Teghil
Keyword(s):  
Laser Irradiation ◽  
Performance Enhancement ◽  
Laser Source ◽  
Thermal Treatments ◽  
Carbon Particles ◽  
Nanosecond Pulsed Laser ◽  
Negative Electrodes ◽  
Before And After ◽  
Hazelnut Shell ◽  
Compositional Changes

Pyrolyzed carbons from bio-waste sources are renewable nanomaterials for sustainable negative electrodes in Li- and Na-ion batteries. Here, carbon derived from a hazelnut shell has been obtained by hydrothermal processing of the bio-waste followed by thermal treatments and laser irradiation in liquid. A non-focused nanosecond pulsed laser source has been used to irradiate pyrolyzed carbon particles suspended in acetonitrile to modify the surface and morphology. Morphological, structural, and compositional changes have been investigated by microscopy, spectroscopy, and diffraction to compare the materials properties after thermal treatments as well as before and after the irradiation. Laser irradiation in acetonitrile induces remarkable alteration in the nanomorphology, increase in the surface area and nitrogen enrichment of the carbon surfaces. These materials alterations are beneficial for the electrochemical performance in lithium half cells as proved by galvanostatic cycling at room temperature.

scholarly journals Modifications of lampbrush chromosome structure of the European domestic goose <i>Anser anser</i>

2012 ◽  
Vol 55 (1) ◽  
pp. 84-96
Author(s):  
K. Andraszek ◽  
E. Smalec
Keyword(s):  
Transcriptional Activity ◽  
Morphological Structure ◽  
Reproductive Period ◽  
Lampbrush Chromosomes ◽  
Anser Anser ◽  
Transcription Activity ◽  
Physiological Processes ◽  
Before And After ◽  
Regulation Changes ◽  
Model Structures

Abstract. Lampbrush chromosomes (LBCs) represent a new model in avian cytogenetics and are increasingly more often used in poultry chromosome analyses. Additionally, lampbrush chromosomes are considered as model structures in the study of transcription regulation. Changes in transcription activity are reflected as modifications of LBC morphological structure and associated with physiological processes in the organism. The aim of the present study was to compare transcriptional activity of the first five lampbrush macrochromosomes and ZW sex lampbrush bivalents sampled from the oocytes of geese prior to and after the reproductive period. The respective bivalents sampled before and after reproduction have similar sizes but differ in morphological structure. Side loops of lampbrush chromosomes are sites of transcription activity. The activity varies according to the loop size. As the loops become more prominent, the activity grows and vice versa. Lampbrush chromosomes sampled after reproduction have smaller side loops. On the other hand, inactive chromomeres become prominent in the chromosomes. Marker loops are the last structures to be degraded after the end of reproduction. Consequently, they are used for identifying particular bivalents at different stages of cellular transcriptional activity.

scholarly journals On a new approach to assessing the energy characteristics of substances

2019 ◽  
Vol 124 ◽  
pp. 01017
Author(s):  
O. S. Sirotkin ◽  
A. M. Pavlova ◽  
R. O. Sirotkin ◽  
A. E. Buntin
Keyword(s):  
Energy Production ◽  
Chemical Interaction ◽  
Heat Of Formation ◽  
Redox Properties ◽  
Chemical Components ◽  
Reaction Products ◽  
Energy Characteristics ◽  
New Approach ◽  
Production Technologies ◽  
The Difference

Within the unified model of chemical bonding and methods of quantitative assessment of components of mixed chemical interaction between the elements in compounds, developed by the authors, a new approach was developed to assess the structural and energy characteristics of substances and fuels. It comprises establishing a correlation between the difference of bonds’ chemical components of reactants and end products. Changes in the chemical bond components affect such characteristics of chemical reactions as the heat of formation of the reaction products, their redox properties, whether reaction is endoor exothermic, as well as the heat of fuel combustion reactions. This approach is an additional reserve for improving the methods for assessing the energy characteristics of fuels and increasing the efficiency of energy production technologies.

scholarly journals EEG Signals Analysis Using Multiscale Entropy for Depth of Anesthesia Monitoring during Surgery through Artificial Neural Networks

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Quan Liu ◽  
Yi-Feng Chen ◽  
Shou-Zen Fan ◽  
Maysam F. Abbod ◽  
Jiann-Shing Shieh
Keyword(s):  
Time Scales ◽  
Multiscale Entropy ◽  
Expert Assessment ◽  
Data Series ◽  
Depth Of Anesthesia ◽  
Eeg Signals ◽  
New Approach ◽  
Before And After ◽  
Artificial Neural ◽  
Different Time Scales

In order to build a reliable index to monitor the depth of anesthesia (DOA), many algorithms have been proposed in recent years, one of which is sample entropy (SampEn), a commonly used and important tool to measure the regularity of data series. However, SampEn only estimates the complexity of signals on one time scale. In this study, a new approach is introduced using multiscale entropy (MSE) considering the structure information over different time scales. The entropy values over different time scales calculated through MSE are applied as the input data to train an artificial neural network (ANN) model using bispectral index (BIS) or expert assessment of conscious level (EACL) as the target. To test the performance of the new index’s sensitivity to artifacts, we compared the results before and after filtration by multivariate empirical mode decomposition (MEMD). The new approach via ANN is utilized in real EEG signals collected from 26 patients before and after filtering by MEMD, respectively; the results show that is a higher correlation between index from the proposed approach and the gold standard compared with SampEn. Moreover, the proposed approach is more structurally robust to noise and artifacts which indicates that it can be used for monitoring the DOA more accurately.

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