scholarly journals Riboflavin deficiency in man: effects on haemoglobin and reduced glutathione in erythrocytes of different ages

1981 ◽  
Vol 46 (2) ◽  
pp. 257-266 ◽  
Author(s):  
Hilary J. Powers ◽  
D. I. Thurnham
Keyword(s):  
Glutathione Reductase ◽  
Density Gradient ◽  
Aspartate Aminotransferase ◽  
Reduced Glutathione ◽  
Riboflavin Deficiency ◽  
Different Ages ◽  
Riboflavin Deficient

1. Erythrocytes (RBC) from control and marginally riboflavin-deficient subjects were fractionated into nine fractions using a discrete density gradient.2. Glutathione reductase (NAD(P)H: glutathione oxidoreductase;EC1.6.4.2) activity and aspartate aminotransferase (EC2.6.1.1) activity (with and without the appropriate co-enzymes) reduced glutathione, methaemoglobin, sulphaemoglobin and oxyhaemoglobin and susceptibility to peroxide were measured in RBC in the different fractions.3. Glutathione reductase and aspartate aminotransferase activities and concentrations of reduced glutathione and oxyhaemoglobin all declined with age, while melhaemoglobin, sulphaemoglobin and susceptibility to peroxide increased with age.4. The only significant differences noted in the RBC from marginally-riboflavin-deficient subjects by comparison with controls, were lower glutathione reductase activities and higher concentrations of methaemoglobin.5. The role of riboflavin in those sytems controlling RBC integrity is discussed.

scholarly journals Glutathione reductase deficiency in Saudi Arabia

1999 ◽  
Vol 5 (6) ◽  
pp. 1208-1212
Author(s):  
A. S. Warsy ◽  
M. A. El Hazmi
Keyword(s):  
Glutathione Reductase ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Normal Functioning ◽  
Riboflavin Deficiency ◽  
Males And Females ◽  
Presence And Absence ◽  
Glutathione Reductase Deficiency ◽  
The Stability ◽  
Riboflavin Deficient ◽  
Reduced Nicotinamide Adenine Dinucleotide

Glutathione reductase [GR]is a ubiquitous enzyme required for the conversion of oxidized glutathione [GSSG] to reduced glutathione [GSH] concomitantly oxidizing reduced nicotinamide adenine dinucleotide phosphate [NADPH]in a reaction essential for the stability and integrity of red cells. Mutations in the GR gene and nutritional deficiency of riboflavin, a co-factor required for the normal functioning of GR, can cause GR deficiency. We conducted a study on 1691 Saudi individuals to determine the overall frequency of GR deficiency and to identify whether the deficiency results from genetic or acquired causes or both. The activity of GR was measured in freshly prepared red cell haemolysate in the presence and absence of flavin adenine dinucleotide [FAD]and the activity coefficient [AC] was determined. Samples with low GR activity [>2.0 IU/g haemoglobin] both in the presence and absence of FAD and an AC between 0.9 and 1.2 were considered GR-deficient. Samples with AC >/= 1.3 were considered riboflavin-deficient. The overall frequency of partial GR deficiency was 24.5% and 20.3% in males and females respectively. In addition, 17.8% of males and 22.4% of females suffered from GR deficiency due to riboflavin deficiency. This could be easily corrected by dietary supplementation with riboflavin. No cases of severe GR deficiency were identified

Bergenin Exhibits Hepatoprotective Activity Against Ethanol-Induced Oxidative Stress in ICR Mice

2019 ◽  
Vol 18 (4) ◽  
pp. 297-302
Author(s):  
Sriset Yollada ◽  
Chatuphonprasert Waranya ◽  
Jarukamjorn Kanokwan
Keyword(s):  
Reactive Oxygen Species ◽  
Gallic Acid ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Hepatic Injury ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Hepatoprotective Activity ◽  
Oxygen Species ◽  
Hepatic Glutathione

Bergenin is a C-glucoside derivative of gallic acid but its antioxidant and hepatoprotective effects have not previously been compared with gallic acid. Male ICR mice were administered bergenin (10, 50, and 250 mg/kg/day) or gallic acid (100 mg/kg/day) for 7 consecutive days before a single administration of ethanol (5 g/kg). Liver sections were histopathologically examined. Aspartate aminotransferase, alanine aminotransferase, reactive oxygen species, and malondialdehyde levels were determined in plasma. Total glutathione, reduced glutathione, and oxidized glutathione levels were determined in liver homogenates. Ethanol induced hepatic injury with prominent histopathological markers including nuclear pyknosis and necrotic areas and this accorded with increases in the plasma levels of aspartate aminotransferase, alanine aminotransferase, reactive oxygen species, and malondialdehyde. Moreover, ethanol disturbed hepatic glutathione homeostasis by reducing glutathione stores. Hepatic injury in the ethanol-induced mice was prevented with bergenin and gallic acid by significant decreases in plasma aspartate aminotransferase, alanine aminotransferase, reactive oxygen species, and malondialdehyde levels and restoration of the hepatic glutathione profile through an increase in the reduced glutathione/oxidized glutathione ratio. Bergenin at 10 mg/kg/day showed comparable hepatoprotective activity to gallic acid in an ethanol-induced mouse model of oxidative stress. Therefore, bergenin might be a promising candidate for further development as a novel hepatoprotective product.

scholarly journals Transkingdom interactions between Lactobacilli and hepatic mitochondria attenuate western diet-induced diabetes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Richard R. Rodrigues ◽  
Manoj Gurung ◽  
Zhipeng Li ◽  
Manuel García-Jaramillo ◽  
Renee Greer ◽  
...  
Keyword(s):  
Functional Role ◽  
Reduced Glutathione ◽  
Western Diet ◽  
Data Driven ◽  
Data Driven Approach ◽  
Probiotic Strains ◽  
Host Metabolism ◽  
Harmful Effects

AbstractWestern diet (WD) is one of the major culprits of metabolic disease including type 2 diabetes (T2D) with gut microbiota playing an important role in modulating effects of the diet. Herein, we use a data-driven approach (Transkingdom Network analysis) to model host-microbiome interactions under WD to infer which members of microbiota contribute to the altered host metabolism. Interrogation of this network pointed to taxa with potential beneficial or harmful effects on host’s metabolism. We then validate the functional role of the predicted bacteria in regulating metabolism and show that they act via different host pathways. Our gene expression and electron microscopy studies show that two species from Lactobacillus genus act upon mitochondria in the liver leading to the improvement of lipid metabolism. Metabolomics analyses revealed that reduced glutathione may mediate these effects. Our study identifies potential probiotic strains for T2D and provides important insights into mechanisms of their action.

The Role of Solid Springer in Masonry Vault

2010 ◽  
Vol 133-134 ◽  
pp. 349-354 ◽  
Author(s):  
Vittorio Ceradini ◽  
Michele Candela ◽  
Roberta Fonti
Keyword(s):  
Scientific Research ◽  
Evolution Process ◽  
Limit Case ◽  
Structural Configuration ◽  
Structural Role ◽  
Different Ages ◽  
Building Component ◽  
And Function ◽  
Technical Rules

During a scientific research, directed to understand the structural role of some particular masonry elements, noticeable in covering structures like vault and dome, we searched the technical rules and function of these elements. We verified that in literature there is no specific documentation about these elements and its mechanic purposes. The study was directed to recognize the most representatives architectures in different ages, and to identify the construction technique’s evolution process of this particular arc-double or thickening of arc that we arrived to identify as a necessary building component to give balance in particular structural configuration. This process put down roots from the roman ancient age, until baroque age, where the most original applications of this regulation were placed. From Pantheon to the limit case of St. Filippo Neri chapel, the covers’ structures springer angle studied was analyzed together with its relation to plan, sections and elevation of all buildings. Therefore, if these elements are well-performed, they follow precise constructive patterns that this article would like to identify and show.

Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells

2013 ◽  
Vol 34 (24) ◽  
pp. 3305-3314 ◽  
Author(s):  
Chiao-Yuan Fan ◽  
Hsiu-Chuan Chou ◽  
Yi-Wen Lo ◽  
Yueh-Feng Wen ◽  
Yi-Chih Tsai ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Glutathione Reductase ◽  
Cancer Cells ◽  
Human Lung ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Human Lung Cancer Cells ◽  
Proteomic Study

Elucidation of the role of glutathione reductase using transgenic plants

1994 ◽  
Vol 22 (4) ◽  
pp. 931-936 ◽  
Author(s):  
Philip Mullineaux ◽  
Gary Creissen ◽  
Pamela Broadbent ◽  
Helen Reynolds ◽  
Baldeep Kular ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Glutathione Reductase

Effect of Riboflavin Deficiency on the Metabolism of Oxypurines in Chicks

1971 ◽  
Vol 49 (12) ◽  
pp. 1059-1062 ◽  
Author(s):  
S. T. Chou
Keyword(s):  
Uric Acid ◽  
Acid Synthesis ◽  
Xanthine Dehydrogenase ◽  
Broiler Chicks ◽  
Uric Acid Concentration ◽  
Riboflavin Deficiency ◽  
Deficient Diet ◽  
High Incidence ◽  
Liver And Kidney ◽  
Riboflavin Deficient

Day-old broiler chicks of both sexes were used in three experiments to determine the effect of riboflavin deficiency on oxypurine metabolism catalyzed by xanthine dehydrogenase, a riboflavin-containing enzyme. Chicks fed a riboflavin-deficient diet (1.38 mg/kg) for 3 weeks exhibited depressed growth and a high incidence of curled-toe paralysis (higher than 80%) as compared to control chicks (15.1 mg riboflavin per kilogram diet; no incidence of curled-toe paralysis). In addition, the precursors of uric acid, hypoxanthine and/or xanthine, accumulated in the liver and kidney of deficient chicks showing curled-toe paralysis. These observations show that dietary riboflavin being incorporated into xanthine dehydrogenase is essential for oxypurine metabolism. Moreover in the chick, the liver and the kidney may be important sites of uric acid synthesis. The low uric acid concentration in the plasma of the deficient chicks appeared to be indicative of a disturbance in uric acid synthesis in the liver and kidney.

Sign in / Sign up

Export Citation Format

Share Document