Alternative culture media and cold-drying for obtaining high biological value Arthrospira platensis (Cyanobacteria)

Phycologia ◽  
2021 ◽  
pp. 1-10
Author(s):  
Elia Bari ◽  
Federico Castillo Cascino ◽  
Luciano Foglio ◽  
Lorenzo Proietti ◽  
Sara Perteghella ◽  
...  
Keyword(s):  
Culture Media ◽  
Arthrospira Platensis ◽  
Biological Value ◽  
Alternative Culture

Six Sigma Methodology in Increasing Spirulina Production

Minerva ◽  
2020 ◽  
Vol 1 (1) ◽  
pp. 23-28
Author(s):  
Daniel Freire ◽  
Omar Flor ◽  
Gabriela Alvarez
Keyword(s):  
Six Sigma ◽  
Culture Media ◽  
Arthrospira Platensis ◽  
Lean Six Sigma ◽  
Growth Monitoring ◽  
Current State ◽  
Physical And Chemical Variables ◽  
Six Sigma Methodology ◽  
Physical And Chemical

This work presents results of improvement in the productivity of Arthrospira platensis (spirulina) in a company dedicated to its production. The six sigma methodology was applied in production processes that require the use of bioreactors. Starting from the analysis of the current state, aspects, physical and chemical variables that directly influence the productivity achieved were identified. Various culture media were tested and subsequently scaled for industrial production. In addition, the incorporation of carbon into the culture medium was controlled, optimizing the range of potential hydrogen pH. The identified parameters were measured and six sigma methodology strategies were assigned. An improvement in productivity corresponding to 66% was verified with the same quality of final product. Keywords: Six sigma, Bioreactors, Productivity, Arthrospira platensis. References [1]E. Ariawan and A. Makalew, “Smart Micro Farm: Sustainable Algae Spirulina Growth Monitoring System” in 10th International Conference on Information Technology and Electrical Engineering (ICITEE), Bali, 2018, pp.1-4. [2]L. Socconini and C. Reato, Lean six sigma: sistema de gestión para liderar empresas. Primera edición. Barcelona: Marge Books, 2019. [3]H. Gutiérrez, Calidad and productividad. Cuarta edición. México D.F.: McGraw-Hill Interamericana, 2014. [4]G. Usharani, P. Saranraj and D. Kanchana, “Spirulina Cultivation: A Review” in International Journal of Pharmaceutical & Biological Archives, vol. 3, no. 6, pp. 1327-1336, December 2012. [5]J. Udin, O. Gani, A. Mahato, I. Sakib and M. Rakiuzzaman, SPIRULINA (Spirulina platensis) PRODUCTION IN DIFFERENT PHOTOBIOREACTORS ON ROOFTOP, International Journal of Business, Social and Scientific Research, vol. 8, no. 1, pp. 15-19, January 2020. [6]M. Arredondo, Contabilidad y análisis de costos. Primera edición. México D.F.: Grupo Editorial Patria, 2015. [7]J. García, Contabilidad de costos. Cuarta edición. México D.F.: McGraw-Hill Interamericana, 2014. [8]L. Socconini, Certificación Lean Six Sigma Green Belt para la excelencia en los negocios. Primera edición. Barcelona: Marge Books, 2015. [9]A. Vian, Introducción a la Química Industrial. Segunda edición. Buenos Aires: Reverté, 2012. [10]S. Milton, Estadística para Biología y Ciencias de la Salud. Tercera edición. Madrid: McGraw-Hill Interamericana, 2014.

scholarly journals Comparison of photoautotrophic and mixotrophic cultivation of microalgae Messastrum gracile (Chlorophyceae) in alternative culture media

2020 ◽  
Vol 80 (4) ◽  
pp. 914-920 ◽  
Author(s):  
L. H. Sipaúba-Tavares ◽  
B. Scardoeli-Truzzi ◽  
D. C. Fenerick ◽  
M. G. Tedesque
Keyword(s):  
Eichhornia Crassipes ◽  
Culture Medium ◽  
Culture Media ◽  
Lemna Minor ◽  
Dry Weight ◽  
Commercial Culture ◽  
Mixotrophic Cultivation ◽  
Lipid Contents ◽  
Alternative Culture ◽  
Chemical Conditions

Abstract Growth and biological conditions of Messastrum gracile were evaluated to compare the effect of photoautotrophic and mixotrophic cultivation on the increase of biomass production and chemical conditions cultured in macrophyte and commercial culture media. The growth rate (k) of M. gracile was different in the culture media, higher in mixotrophic cultivation for Lemna minor culture medium, whilst to Eichhornia crassipes and NPK culture media were higher in photoautotrophic cultivation. Mean lipid contents in photoautotrophic cultivation were 8.2% biomass dry weight, whereas they reached 19% biomass dry weight in mixotrophic cultivation. Protein contents were below 48% biomass dry weight in photoautotrophic cultivation and 30% biomass dry weight in mixotrophic cultivation. Messastrum gracile cultured in macrophyte culture media (E. crassipes and L. minor) and NPK culture medium provided satisfactory results with regard to lipid and protein contents in mixotrophic and photoautotrophic cultivations, respectively. Lipid and protein contents in alternative media were higher or similar to the CHU12 commercial culture medium.

scholarly journals Urban secondary sewage: an alternative medium for the culture of Tetraselmis chuii (Prasinophyceae) and Dunaliella viridis (Chlorophyceae)

2004 ◽  
Vol 47 (3) ◽  
pp. 451-459 ◽  
Author(s):  
Rauquírio André Albuquerque Marinho da Costa ◽  
Maria Luise Koening ◽  
Sílvio José de Macedo
Keyword(s):  
Culture Media ◽  
Chicken Manure ◽  
Dunaliella Viridis ◽  
Chi Square ◽  
Nutritive Medium ◽  
Tetraselmis Chuii ◽  
Alternative Culture ◽  
Chi Square Test ◽  
Cell Densities ◽  
Intensive Aquaculture

The effect of different concentrations (10, 20, 30 and 40%) of urban secondary sewage on the growth of Tetraselmis chuii (Prasinophyceae) and Dunaliella viridis (Chlorophyceae) was examined to verify the possibility of its use as an alternative culture medium for these species. Stocks and 700mL duplicate batch cultures were maintained under controlled laboratory conditions. 30% and 40% additions were the most efficient for the growth of both species. Statistical analysis of the adjusted growth (cell.mL-1) and biomass (µg chlorophyll-a.mL-1) curves performed using the Chi-square test (p<0.05) demonstrated significant differences between the nutritive medium f/2 and the 40% addition used for these two species. The results suggested that some of the concentrations tested here yielded satisfactory cell densities and growth rates when compared with other culture media (macroalgae, bovine and chicken manure extracts). Thus it could be used by semi intensive aquaculture systems.

scholarly journals Low sensitivity of conventional fungal agars in fungemia by Rhodotorula mucilaginosa: description of two cases

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Carmen Alicia Garcia-Gutiérrez ◽  
María Soledad Cuétara-García ◽  
María Dolores Moragues ◽  
Jorge Ligero ◽  
Sara María Quevedo ◽  
...  
Keyword(s):  
Culture Media ◽  
Early Recognition ◽  
Correct Diagnosis ◽  
Common Species ◽  
Diagnostic Tools ◽  
Rhodotorula Mucilaginosa ◽  
Alternative Culture ◽  
Panfungal Pcr ◽  
Low Sensitivity ◽  
Specific Symptoms

Abstract Background Although most bloodstream yeast infections are caused by Candida spp., infections by rare or less common species have increased in recent years. Diagnosis of infections caused by these species is difficult due to the lack of specific symptoms and adequate diagnostic tools. Cases presentation We describe two cases of fungemia by Rhodotorula mucilaginosa within a few months of each other, in a secondary Spanish hospital. In both cases, diagnosis was challenging. Blood subcultures in conventional fungal media were persistently negatives and the use of non-conventional fungal media was essential for isolating the yeasts and achieving a correct diagnosis. 1–3 beta-d-glucan detection and a panfungal PCR assay were helpful techniques to confirm the diagnosis Conclusion It is highly important to establish an early diagnosis for fungemia. The process is challenging because often non-specific symptoms are presents. When yeasts grow in blood cultures other genera than Candida spp. could be the cause of infection. Patient risk factors should be assessed to incorporate alternative culture media and the available rapid diagnostic test, in order to provide an early recognition of the pathogen.

scholarly journals Effects of Liquid Digestate Treatment on Sustainable Microalgae Biomass Production

2021 ◽  
Author(s):  
Marta Kisielewska ◽  
Marcin Dębowski ◽  
Marcin Zieliński ◽  
Joanna Kazimierowicz ◽  
Piera Quattrocelli ◽  
...  
Keyword(s):  
Growth Medium ◽  
Biomass Production ◽  
Culture Medium ◽  
Culture Media ◽  
Biomass Concentration ◽  
Arthrospira Platensis ◽  
Microalgal Biomass ◽  
Liquid Digestate ◽  
Microalgae Biomass ◽  
High Treatment

AbstractThe aim of the study was to investigate the potential of microalgal cultivation on anaerobic liquid digestate as a growth medium. The two methods of liquid digestate treatment including centrifugation and distillation and the two algal strains (Chlorella vulgaris and Arthrospira platensis) were compared. Additionally, the volume of the liquid digestate used to prepare the culture medium constituted from 10 to 50% of the medium volume. The study demonstrated that the highest C. vulgaris and A. platensis biomass productions of 2490 mg TS/L and 2990 mg/L, respectively, were obtained by adding 50% of distilled digestate to a growth medium. Regarding centrifuged liquid digestate, only 10% dilution was required to obtain the maximum final biomass concentration. A. platensis removed 81.1% and 66.4% of the total nitrogen from medium prepared on distilled and centrifuged digestate, respectively, while C. vulgaris ensured 64.1% and 47.1% of removal, respectively. The phosphorus removal from both culture media was higher than 94.2% with A. platensis, while it was 70.4% from distilled and 87.4% from centrifuged media with C. vulgaris. The study confirmed a great potential of microalgal biomass production on anaerobic liquid digestate with a high treatment efficiency of digestate.

scholarly journals Estandarización de un medio de cultivo complejo para la multiplicación de la cepa C50 de Rhizobium sp.

2009 ◽  
Vol 10 (1) ◽  
pp. 70 ◽  
Author(s):  
Daniel Fernando Rojas T. ◽  
María Fernanda Garrido R. ◽  
Ruth Rebeca Bonilla B.
Keyword(s):  
Yeast Extract ◽  
Agricultural Production ◽  
Sustainable Management ◽  
Culture Media ◽  
Media Culture ◽  
Traditional Media ◽  
Yeast Extract Mannitol ◽  
Alternative Culture ◽  
Statistical Designs ◽  
Complex Culture

<p>El uso indiscriminado de fertilizantes químicos y su proceso de obtención y aplicación ha incrementado los costos de producción agrícola y los problemas ambientales debido a la contaminación del aire, el suelo y las aguas. Se ha planteado como alternativa la aplicación de fertilizantes biológicos como una herramienta económica y limpia para el manejo sostenible de los ecosistemas. Sin embargo, en los procesos de escalamiento de un biofertilizante pueden incrementarse los costos de producción debido al tipo de formulación y a los medios de cultivo empleados para la multiplicación de las bacterias. Esta investigación, con base en el uso secuencial de diseños estadísticos, presenta la estandarización de un medio de cultivo económico para la multiplicación de la cepa C50 de <em>Rhizobium </em>sp. De ocho fuentes nutricionales se seleccionaron cinco, teniendo como criterios los menores costos económicos y la disponibilidad de dichas fuentes; de estas cinco, tres influyeron significativamente sobre el desarrollo de la cepa. La composición optimizada del medio alterno incluyó glicerol, melaza, glutamato, extracto de levadura y sales. No se presentaron diferencias significativas en el crecimiento de la cepa C50 en el medio alterno comparado con el tradicional (levadura-manitol), ni en la viabilidad de la cepa crecida en el medio tradicional respecto al alterno, cuando se inoculó sobre turba. El inoculante conservó su calidad en refrigeración durante 30 días. Las cepas J01, T14 y C2 mostraron buen índice de crecimiento sobre el medio alterno; no se presentaron diferencias significativas en los recuentos entre las cepas J01 y T14, mientras que la cepa C2 creció mejor en el medio alterno.  </p><p><strong><br /></strong></p><p><strong>Standardization of a complex culture media for multiplication of C50 <em>Rhizobium </em>sp. strain</strong> </p>The indiscriminate use of chemical fertilizers, its process of production and its application had increased the costs of agricultural production and the environmental problems because of the pollution of air, soil and water sources. The alternative is the use of biological fertilizers as an economical and clean tool in order to sustainable management of ecosystems. However, in the escalation processes of a biofertilizer the costs of production could increase due to the type of formulation and the culture media used for the multiplication of the bacteria. This research, by using statistical designs sequentially, presents the standardization of an economical culture media used to multiply the strain C50 of <em>Rhizobium </em>sp. From eight nutritional sources, five were selected taking as criteria the low economical costs and the availability of above mentioned nutritional sources; from these five sources, three of them influenced the development of the strain significantly. The optimized composition of the alternative culture media included glycerol, molasses, glutamate, yeast extract and salts. There were no significantly differences in the growth of the strain C50 in the alternative culture media compared with the traditional one (yeast extract – mannitol); nor in viability of the strain growth on traditional media culture compared with the alternative one, when it was inoculated on peat. The inoculants conserved its quality in refrigeration during 30 days. The strains J01, T14 and C2 showed a better rate of growth on the alternative culture media; it was not show significant differences in the counts between the strains J01 and T14; whereas, the strain C2 growth better in the alternative culture media.

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