scholarly journals Comparison of photoautotrophic and mixotrophic cultivation of microalgae Messastrum gracile (Chlorophyceae) in alternative culture media

2020 ◽  
Vol 80 (4) ◽  
pp. 914-920 ◽  
Author(s):  
L. H. Sipaúba-Tavares ◽  
B. Scardoeli-Truzzi ◽  
D. C. Fenerick ◽  
M. G. Tedesque
Keyword(s):  
Eichhornia Crassipes ◽  
Culture Medium ◽  
Culture Media ◽  
Lemna Minor ◽  
Dry Weight ◽  
Commercial Culture ◽  
Mixotrophic Cultivation ◽  
Lipid Contents ◽  
Alternative Culture ◽  
Chemical Conditions

Abstract Growth and biological conditions of Messastrum gracile were evaluated to compare the effect of photoautotrophic and mixotrophic cultivation on the increase of biomass production and chemical conditions cultured in macrophyte and commercial culture media. The growth rate (k) of M. gracile was different in the culture media, higher in mixotrophic cultivation for Lemna minor culture medium, whilst to Eichhornia crassipes and NPK culture media were higher in photoautotrophic cultivation. Mean lipid contents in photoautotrophic cultivation were 8.2% biomass dry weight, whereas they reached 19% biomass dry weight in mixotrophic cultivation. Protein contents were below 48% biomass dry weight in photoautotrophic cultivation and 30% biomass dry weight in mixotrophic cultivation. Messastrum gracile cultured in macrophyte culture media (E. crassipes and L. minor) and NPK culture medium provided satisfactory results with regard to lipid and protein contents in mixotrophic and photoautotrophic cultivations, respectively. Lipid and protein contents in alternative media were higher or similar to the CHU12 commercial culture medium.

scholarly journals Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte

2017 ◽  
Vol 29 (0) ◽  
Author(s):  
Lúcia Helena Sipaúba-Tavares ◽  
Alexandra Maria Donadon Lusser Segali ◽  
Flavia Almeida Berchielli-Morais ◽  
Bruno Scardoeli-Truzzi
Keyword(s):  
Growth Rate ◽  
Eichhornia Crassipes ◽  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
Nutritional Composition ◽  
Inorganic Fertilizer ◽  
Lipid Contents ◽  
Commercial Medium ◽  
The Media

Abstract Aim: The influence of three different culture media on the growth and biochemical composition of Ankistrodemsus gracilis microalgae is evaluated. Methods Microalgae were placed in a bath culture during 28 days, with inorganic fertilizer (NPK) and macrophyte Eichhornia crassipes as culture medium to compare the growth of microalgae and commercial medium CHU12. Protein and lipid contents, water conditions of the culture medium and physiological parameters were assessed weekly. Results Growth rate, macro-minerals, micro-nutrients and nutritional values were similar to commercial culture medium in the media with inorganic fertilizer and macrophyte. The biological indexes of microalgae were not influenced by weekly differences during the study period. Although lipid contents, nitrogen and growth rate of A. gracilis were higher in inorganic fertilizer and macrophyte media; Mn, B, Cu, S, dry matter, ash content, cell volume, TOC and total length were similar among the media used. The same high cell density 25 x 105 cells.mL-1 was obtained in NPK and in CHU12 medium respectively after 11 and 14 days cultivation. Conclusion Culture media inorganic fertilizer (NPK) and macrophyte (Eichhornia crassipes), were adequate and may replace the commercial medium CHU12 for the cultivation of microalgae A. gracilis, resulting in high nutritional composition, high biomass, high nutrients and low-cost.

scholarly journals MODIFICAÇÕES NO MEIO DE CULTURA, FOTOPERÍODO E TEMPO DE CULTIVO AFETAM O ALONGAMENTO E ENRAIZAMENTO IN VITRO DE BANANEIRA CV. PACOVAN

Nativa ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Marcos Vinícius Marques Pinheiro ◽  
Ana Cristina Portugal Pinto De Carvalho ◽  
Fabrina Bolzan Martins
Keyword(s):  
Plant Height ◽  
Culture Medium ◽  
Culture Media ◽  
Dry Weight ◽  
Salt Mixture ◽  
Musa Spp ◽  
Fresh Biomass ◽  
Shoot Dry Weight ◽  
Number Of Leaves

No intuito de elevar as taxas de sobrevivência durante a etapa de aclimatização e posterior plantio a campo, avaliou-se o enraizamento in vitro de bananeira cv. Pacovan, em diferentes concentrações de sais MS e de sacarose. Utilizou-se DIC, esquema fatorial (6x2x3), com seis meios de cultura [sendo três concentrações de nutrientes do meio MS (100%; 50% de macronutrientes; e 50% dos sais macro e micronutrientes), e duas concentrações de sacarose (1,5/3,0%)], dois fotoperíodos (12/16 h) e três tempos de cultivo (21, 28 ou 35 dias) e seis repetições/tratamento. Analisaram-se: altura da planta, número de folhas/planta, massas frescas e secas das partes aérea e radicular. Para altura da planta, massa fresca da parte aérea e radicular, o meio MS 50% dos sais + sacarose (1,5%) com fotoperíodo de 16 h e tempo de cultivo de 35 dias foi satisfatório. Para massa seca da parte aérea foi MS 50% de sais + sacarose (3%), e para massa seca da parte radicular, MS 100% + sacarose (3%) (em 12hs/28 dias e 16hs/21 dias). Para o alongamento/enraizamento in vitro da bananeira cv. Pacovan sugere-se MS 50% de sais (macro e micronutrientes), redução ou manutenção de sacarose (1,5 ou 3%) em 16h/35 dias de cultivo.Palavra-chave: Musa spp., propagação in vitro, sistema radicular. CHANGES IN CULTURE MEDIUM, PHOTOPERIOD AND TIME OF CULTIVATION AFFECT THE IN VITRO ELONGATION AND ROOTING OF BANANA CV. PACOVAN ABSTRACT:In order to achieve high rates of survival during the acclimatization and later planting in the field, was evaluated the in vitro of banana cv. Pacovan plants under different concentrations of sucrose and MS basal salt mixture. The experiment was assembled in a DIC, in 6x2x3, six different culture media [three different MS salt mixture concentrations (100%; 50% of macronutrients; and 50% of macro/micronutrients) and two sucrose concentrations (1.5/3%)], two photoperiods (12/16 hours) and three cultivation times (21, 28 or 35 days). Each treatment was composed by 6 replicates. Plant height, number of leaves/plant, fresh and dry weight of roots and shoots, were analyzed. Satisfactory results for plant height and shoot and root fresh biomass were observed in MS with macro/micronutrients (50%) + sucrose (3%), 16 hours/35 days. The highest values of shoot dry weight were observed in MS with macro/micronutrients (50%) + sucrose (3%); the highest root dry weight was achieved with MS 100% + sucrose (3%) (12hs/28 and 16hs/21 days). The suggested medium for the in vitro elongation and rooting stage of banana cv. Pacovan is the MS with 50% of salts (macro and micronutrients), reduction or maintenance of sucrose (1.5 or 3%) in 16h/35 days of cultivation.Keywords: Musa spp., in vitro propagation, root system. DOI:

scholarly journals Preparation of an experimental low-fluoride diet from single-cell organisms for rats and mice

1980 ◽  
Vol 44 (3) ◽  
pp. 371-380 ◽  
Author(s):  
S. A. Khalawan ◽  
J. C. Elliott ◽  
R. W. Fearnhead
Keyword(s):  
Single Cell ◽  
Culture Medium ◽  
Culture Media ◽  
Dry Weight ◽  
Chlorella Pyrenoidosa ◽  
Distilled Water ◽  
Yeast Biomass ◽  
Two Generations ◽  
Rats And Mice ◽  
Maize Oil

1. A method for producing a standard low-fluoride diet from a green alga and yeast is described. Chlorella pyrenoidosa was grown in a culture medium prepared with distilled water and analytical grade chemical salts. The spent culture medium from the alga culture was reclaimed and replenished with salts and sucrose for the production of yeast, Saccharomyces cerevisiae.2. The single-cell organisms were separated by centrifugation from their culture media and the dried cells were blended with sucrose, maize oil, cellulose and a salt mix to produce diet pellets for rats and mice.3. The diet was readily accepted as food by rats and mice and it was found to contain 100–300 μg fluoride/kg dry weight. Two generations of rats and four generations of mice were bred on this diet.4. The use of hydroxyapatite to reduce the fluoride content of the chemicals used in the production of the alga and yeast biomass was investigated. Diet pellets prepared with this biomass contained 45–60 μg fluoride/kg dry weight.

scholarly journals Short communication: The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis

2018 ◽  
Vol 26 (3) ◽  
pp. eSC05 ◽  
Author(s):  
Catia Pereira ◽  
Itziar A. Montalbán ◽  
Tomás Goicoa ◽  
M. Dolores Ugarte ◽  
Sandra Correia ◽  
...  
Keyword(s):  
Culture Medium ◽  
Short Communication ◽  
Pinus Halepensis ◽  
Culture Media ◽  
Aleppo Pine ◽  
Embryogenic Cell ◽  
Plantlet Production ◽  
Embryogenic Cell Lines ◽  
Chemical Conditions ◽  
Physical And Chemical

Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis.Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain).Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage.Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages.Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability. 

scholarly journals Aquaculture biological waste as culture medium to cultivation of Ankistrodesmus gracilis (Reinsch) Korshikov

2017 ◽  
Vol 78 (3) ◽  
pp. 579-587 ◽  
Author(s):  
L. H. Sipaúba-Tavares ◽  
T. Florêncio ◽  
B. Scardoeli-Truzzi
Keyword(s):  
Chlorophyll A ◽  
Cell Density ◽  
Total Phosphorus ◽  
Biochemical Composition ◽  
Eichhornia Crassipes ◽  
Culture Medium ◽  
Culture Media ◽  
Conventional Culture ◽  
Significant Difference ◽  
Biological Wastes

Abstract Current study investigated the effectiveness of different macrophytes as culture media for Ankistrodesmus gracilis in laboratory conditions. Significant difference (p < 0.05) was reported in cell density with regard to conventional culture medium (CHU12) and macrophytes culture media. Mean cell density in NPK, Eichhornia crassipes and E. azurea media was higher (p < 0.05) than in conventional culture medium. Chlorophyll-a was higher than 1 g.L-1, except in CHU12 (0.7 ± 0.4 g.L-1) and T. domingensis (0.8 ± 0.3 g.L-1) media. Nitrate decreased sharply as from the 7th-day of the experiment. Ammonium and total phosphorus were highest in culture media and ranged between 0.4 g.L-1 (P. cordata medium) and 1.7 g.L-1 (CHU12 medium) for ammonium, and between 0.8 g.L-1 (CHU12 medium) and 1.9 g.L-1 (T. domingensis medium) for total phosphorus. Results revealed inorganic fertilizer and macrophytes combined with vitamins may be effective as culture media and strongly supports the growth of Ankistrodesmus gracilis, since cell density and biochemical composition are similar to or higher than conventional culture medium (CHU12). Macrophyte is a tool for aquaculture since biological wastes may be used with nutrients to improve the cultivation of microalgae.

scholarly journals Orchid in vitro growth as affected by nitrogen levels in the culture medium

2012 ◽  
Vol 30 (1) ◽  
pp. 119-124 ◽  
Author(s):  
Armando R Tavares ◽  
Jorge Luiz M Young ◽  
Sandra S Ori ◽  
Shoey Kanashiro ◽  
Giuseppina PP Lima ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Nitrogen Concentration ◽  
Dry Weight ◽  
In Vitro Cultivation ◽  
Ionic Balance ◽  
In Vitro Growth ◽  
Nitrogen Levels ◽  
Nitrogen Concentrations

In vitro cultivation is the main propagation method for the family Orchidaceae, whereas nitrogen is the most important nutrient in the culture media. This work was carried out to study the influence of different nitrogen concentrations on the in vitro growth of the orchid Phalaenopsis amabilis. Nitrogen concentrations varied by altering the ionic balance of the Murashige & Skoog (MS) culture medium. Plants, 360 days old, were cultivated in liquid MS, modified with 7.5, 15, 30, 45, and 60 mM N. After 180 days, we assessed plant and root length, number of leaves and roots, and fresh and dry weight of leaves, roots and plants. Treatments were assigned to completely randomized plots, with four replications. Plots consisted of five three-plant flasks. The lowest nitrogen level (7.5 mM) in the medium induced root development in length, number, and fresh and dry weight. The concentration 30 mM N stimulated both emission and dry weight accumulation of leaves. The original nitrogen concentration in the MS medium (60 mM) was excessive for the in vitro growth of P. amabilis.

BIOSYNTHESIS OF RIBOFLAVIN BY EREMOTHECIUM ASHBYII: IX. GROWTH AND RIBOFLAVIN FORMATION AND THEIR RELATION TO THE UTILIZATION AND ASSIMILATION OF THE CONSTITUENTS OF THE LIQUID CULTURE MEDIA

1965 ◽  
Vol 11 (4) ◽  
pp. 625-628 ◽  
Author(s):  
H. G. Osman ◽  
M. S. Chenouda
Keyword(s):  
Carbon Source ◽  
Total Nitrogen ◽  
Lipid Content ◽  
Liquid Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Total Lipid Content ◽  
Dry Weight ◽  
Mature Stage ◽  
Lactic Acids

The major amount of riboflavin is formed when the mycelia reach a mature stage and the major carbon source is almost exhausted. While the riboflavin is being synthesized in larger quantities, the mycelial dry weight, the total nitrogen, and total lipid content decrease. The mobilized cell reserves may be those components which call upon the biosynthesis of the major amount of the vitamin. At the stage of growth where glucose is almost completely utilized an increase in the excretion of pyruvic and lactic acids from the mycelia into the culture medium occurs. This may partly explain the increase in the acidity of the culture medium at that stage.

scholarly journals Manganese (Mn) and Lead (Pb) Content in Lemna Culture Fertilized by Bioslurry

2020 ◽  
pp. 1-6
Author(s):  
Iskandar . ◽  
Yuli Andriani ◽  
Irfan Zidni
Keyword(s):  
Experimental Method ◽  
Culture Medium ◽  
Culture Media ◽  
Lemna Minor ◽  
Small Water ◽  
Randomized Design ◽  
Water Plant ◽  
Mn Content ◽  
Completely Randomized Design ◽  
Experimental Pond

Lemna minor is a small water plant that floats on water and has the potential to be fresh feed for fish. Bioslurry is a by-product of the management of biogas from livestock manure which is often used as fertilizer for plants because it contains nutrients in a readily available form. Among the contents contained in bioslurry, manganese and lead are important things related to their use for plants and fish. This study aims to determine the mineral (Mn) and lead (Pb) content in the medium of lemna cultivation which is fertilized with various doses of bioslurry. The study was conducted at the Ciparanje Experimental Pond, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Indonesia. This study used an experimental method with Completely Randomized Design (CRD) with four treatments with three replications. The treatment in the form of adding bioslurry to the lemna culture media was 0.00%, 0.25%, 1.00% and 2.50% of the volume of water. The minor density of Lemna used is 800 g/m2. The culture was carried out in 4 cycles for 7 days. The parameters observed were Mn and Pb content in the lemna culture medium shortly after the addition of Bioslurry and after 7 days of lemna cultivation. Based on the results of the study, the use of 2.5% bioslurry concentration can produce the highest Mn content with a range of 2.0-4.6 ppm and 2.92 ppm Pb content after 7 days of lemna cultivation. In addition, the use of 2.5% bioslurry concentration resulted in Mn and Pb values in the culture medium shortly after stirring bioslurry in the lemna culture media. Based on the research results, the treatment of adding 2.5% bioslurry to the lemna culture media is the best dose for produced Mn for fish, as well as the safest Pb for aquaculture activities.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

Eliminating Thrips in Microshoot Cultures

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506d-506
Author(s):  
Robert R. Tripepi ◽  
Holly J. Schwager ◽  
Mary W. George ◽  
Joseph P. McCaffrey
Keyword(s):  
Betula Pendula ◽  
Culture Media ◽  
Dry Weight ◽  
Thrips Tabaci ◽  
Shoot Cultures ◽  
Onion Thrips ◽  
Ulmus Americana ◽  
Shoot Height ◽  
Tissue Culture Media ◽  
Plant Tissue Cultures

Two insecticides, acephate or azadirachtin, were added to tissue culture media to determine their effectiveness in controlling onion thrips (Thrips tabaci Lindeman.) and to determine if these insecticides could damage the plant shoot cultures. To test for insecticide phytotoxicity, microshoots from European birch (Betula pendula), American elm (Ulmus americana), `Pink Arola' chrysanthemum (Dendranthema grandiflora), `America' rhododendron (Rhododendron catawbiense), `Golden Emblem' rose (Rosa hybrida), and `Gala' apple (Malus domestica) were placed in 130-ml baby food jars containing 25 ml of medium supplemented with 6.5, 13, or 26 mg/l Orthene® (contained acephate) or 0.55, 1.1, or 2.2 ml/l Azatin® (contained azadirachtin). Control jars lacked insecticide. To test for thrips control, 13 mg/l Orthene® or 0.55 ml/l Azatin® was added to Murashige and Skoog medium, and 10 thrips were placed on `Gala' apple microshoots in each jar. Jars were sealed with plastic wrap. In both studies, microshoot dry weight and heights were determined. In the second study, the total number of thrips per jar was also determined 3 weeks after inoculation. Microshoots on Orthene®-treated media lacked phytotoxicity symptoms, regardless of the concentration used. In contrast, Azatin® hindered plant growth, decreasing shoot height or dry weight by up to 85% depending on the species. Both insecticides prevented thrips populations from increasing, since less than 10 thrips were found in jars with insecticide-treated medium. Control jars, however, contained an average of almost 70 thrips per jar. This study demonstrated that both Orthene® and Azatin® were effective for eradicating thrips from plant tissue cultures, but Orthene® should probably be used because Azatin® was phytotoxic to all species tested.

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