scholarly journals Estandarización de un medio de cultivo complejo para la multiplicación de la cepa C50 de Rhizobium sp.

2009 ◽  
Vol 10 (1) ◽  
pp. 70 ◽  
Author(s):  
Daniel Fernando Rojas T. ◽  
María Fernanda Garrido R. ◽  
Ruth Rebeca Bonilla B.
Keyword(s):  
Yeast Extract ◽  
Agricultural Production ◽  
Sustainable Management ◽  
Culture Media ◽  
Media Culture ◽  
Traditional Media ◽  
Yeast Extract Mannitol ◽  
Alternative Culture ◽  
Statistical Designs ◽  
Complex Culture

<p>El uso indiscriminado de fertilizantes químicos y su proceso de obtención y aplicación ha incrementado los costos de producción agrícola y los problemas ambientales debido a la contaminación del aire, el suelo y las aguas. Se ha planteado como alternativa la aplicación de fertilizantes biológicos como una herramienta económica y limpia para el manejo sostenible de los ecosistemas. Sin embargo, en los procesos de escalamiento de un biofertilizante pueden incrementarse los costos de producción debido al tipo de formulación y a los medios de cultivo empleados para la multiplicación de las bacterias. Esta investigación, con base en el uso secuencial de diseños estadísticos, presenta la estandarización de un medio de cultivo económico para la multiplicación de la cepa C50 de <em>Rhizobium </em>sp. De ocho fuentes nutricionales se seleccionaron cinco, teniendo como criterios los menores costos económicos y la disponibilidad de dichas fuentes; de estas cinco, tres influyeron significativamente sobre el desarrollo de la cepa. La composición optimizada del medio alterno incluyó glicerol, melaza, glutamato, extracto de levadura y sales. No se presentaron diferencias significativas en el crecimiento de la cepa C50 en el medio alterno comparado con el tradicional (levadura-manitol), ni en la viabilidad de la cepa crecida en el medio tradicional respecto al alterno, cuando se inoculó sobre turba. El inoculante conservó su calidad en refrigeración durante 30 días. Las cepas J01, T14 y C2 mostraron buen índice de crecimiento sobre el medio alterno; no se presentaron diferencias significativas en los recuentos entre las cepas J01 y T14, mientras que la cepa C2 creció mejor en el medio alterno.  </p><p><strong><br /></strong></p><p><strong>Standardization of a complex culture media for multiplication of C50 <em>Rhizobium </em>sp. strain</strong> </p>The indiscriminate use of chemical fertilizers, its process of production and its application had increased the costs of agricultural production and the environmental problems because of the pollution of air, soil and water sources. The alternative is the use of biological fertilizers as an economical and clean tool in order to sustainable management of ecosystems. However, in the escalation processes of a biofertilizer the costs of production could increase due to the type of formulation and the culture media used for the multiplication of the bacteria. This research, by using statistical designs sequentially, presents the standardization of an economical culture media used to multiply the strain C50 of <em>Rhizobium </em>sp. From eight nutritional sources, five were selected taking as criteria the low economical costs and the availability of above mentioned nutritional sources; from these five sources, three of them influenced the development of the strain significantly. The optimized composition of the alternative culture media included glycerol, molasses, glutamate, yeast extract and salts. There were no significantly differences in the growth of the strain C50 in the alternative culture media compared with the traditional one (yeast extract – mannitol); nor in viability of the strain growth on traditional media culture compared with the alternative one, when it was inoculated on peat. The inoculants conserved its quality in refrigeration during 30 days. The strains J01, T14 and C2 showed a better rate of growth on the alternative culture media; it was not show significant differences in the counts between the strains J01 and T14; whereas, the strain C2 growth better in the alternative culture media.

scholarly journals Desarrollo y evaluación de un medio de cultivo alternativo para la multiplicación de Azospirillum brasilense C16 mediante diseños estadísticos secuenciados

2013 ◽  
Vol 13 (2) ◽  
pp. 201 ◽  
Author(s):  
Andrés Moreno-Galván ◽  
Daniel F. Rojas-Tapias ◽  
Ruth Bonilla
Keyword(s):  
Yeast Extract ◽  
Azospirillum Brasilense ◽  
Culture Medium ◽  
Medium Composition ◽  
High Yield ◽  
Steepest Ascent ◽  
Statistical Experiments ◽  
Alternative Culture ◽  
Plant Growth Promoting ◽  
Statistical Designs

<p>Para la producción masiva de inoculantes basados en bacterias promotoras de crecimiento vegetal (PGPR), es fundamental un medio de cultivo de alto rendimiento. La aplicación secuenciada de diseños estadísticos fue usada para optimizar la producción de biomasa de Azospirillum brasilense C16, seis fuentes nutricionales (glicerol, glutamato, manitol, acido cítrico, extracto de levadura y K2HPO4 3H2O) y tres fuentes minerales (MgSO4 7H2O, FeCl3 y NaCl) fueron evaluadas mediante cinco experimentos estadísticos - Placket-Burman, factorial fraccionado, diseño de paso ascendente, análisis de superficie de respuesta y screening mineral, para tal efecto. La composición optimizada del medio (g L-1) fue: 28,33 glutamato, 2,92 extracto de levadura, 1,34 K2HPO4 3H2O, 0,5 MgSO4 7H2O y 0,02 FeCl3, la cual luego de 24 h de incubación permitió producir una cantidad de proteína (32,04 μg) y biomasa seca (1,51 g L-1) del 1,72 y 1,68 veces más alta, respectivamente, en relación al medio de cultivo convencional. </p><p> </p><p><strong>Development and evaluation of an alternative culture medium for mass cultivation of <em>Azospirillum brasilense </em>C16 using sequential statistical Designs </strong></p><p>For mass plant growth-promoting inoculant production, a high-yield culture medium is fundamental. Sequential application of statistical designs was used to optimize <em>Azospirillum brasilense </em>C16 biomass production. Six nutritional (glycerol, glutamate, mannitol, citric acid, yeast extract and K2HPO4 3H2O) and three mineral sources (MgSO4 7H2O, FeCl3 and NaCl) were evaluated using five statistical experiments Placket-Burman, factorial design, steepest ascent, response surface analysis, and mineral screening. The optimum medium composition (g L-1) was as follows: 28.33 glutamate, 2.92 yeast extract, 1.34 K2HPO4 3H2O, 0.5 MgSO4 7H2O and 0.02 FeCl3. After 24 hours of incubation, protein (32.04 μg) and dry biomass (1.51 g L-1) were 1.72 and 1.68 times higher than in conventional growth medium. </p>

Roquefortine C Occurrence in Blue Cheese

2001 ◽  
Vol 64 (2) ◽  
pp. 246-251 ◽  
Author(s):  
CARLO FINOLI ◽  
ANGELA VECCHIO ◽  
ANTONIETTA GALLI ◽  
IVAN DRAGONI
Keyword(s):  
Yeast Extract ◽  
Culture Media ◽  
Penicillium Roqueforti ◽  
Blue Cheese ◽  
Penicillium Crustosum ◽  
Sucrose Medium ◽  
Low Toxicity ◽  
Yeast Extract Sucrose ◽  
Roquefortine C

Several strains of Penicillium are used for the production of mold-ripened cheeses, and some of them are able to produce mycotoxins. The aims of the research were the determination of roquefortine C and PR toxin in domestic and imported blue cheeses, the identification of the penicillia used as starter, and the investigation of their capacity for producing toxins in culture media. Roquefortine C was always found in the cheeses at levels ranging from 0.05 to 1.47 mg/kg, whereas the PR toxin was never found. The identification of the fungal strains present in the domestic cheeses included Penicillium glabrum, Penicillium roqueforti, and Penicillium cyclopium in the Gorgonzola “dolce” and Penicillium roqueforti in the Gorgonzola “naturale”; in one case, the presence of Penicillium crustosum was observed. The strains isolated from the foreign cheeses belonged to P. roqueforti. The strains were able to produce between 0.18 and 8.44 mg/liter of roquefortine in yeast extract sucrose medium and between 0.06 and 3.08 mg/liter and less than 0.05 mg/liter when inoculated in milk at 20°C for 14 days and 4°C for 24 days, respectively. Linear relations between production of roquefortine in culture media and cheeses did not emerge. PR toxin ranged from less than 0.05 to 60.30 mg/liter in yeast extract sucrose medium and was produced in milk at 20°C from only one strain. The low levels and the relatively low toxicity of roquefortine make the consumption of blue cheese safe for the consumer.

Breaking limitations of complex culture media: Functional non-viral miRNA delivery into pharmaceutical production cell lines

2013 ◽  
Vol 168 (4) ◽  
pp. 589-600 ◽  
Author(s):  
Simon Fischer ◽  
Andreas Wagner ◽  
Aron Kos ◽  
Armaz Aschrafi ◽  
René Handrick ◽  
...  
Keyword(s):  
Cell Lines ◽  
Culture Media ◽  
Complex Culture ◽  
Pharmaceutical Production ◽  
Production Cell

Studies on growth and symbiotic nitrogen fixation of Rhizobium of Vigna radiata under stress conditions

1984 ◽  
Vol 102 (2) ◽  
pp. 399-404
Author(s):  
R. Rai ◽  
V. Prasad
Keyword(s):  
Nitrogen Fixation ◽  
Yeast Extract ◽  
Vigna Radiata ◽  
Symbiotic Nitrogen Fixation ◽  
Alkaline Media ◽  
Growth Behaviour ◽  
Soil Stress ◽  
Wide Ph Range ◽  
Yeast Extract Mannitol ◽  
Ph Range

SummaryOne fast-growing acid-producing Rhizobium strain 995 of Vigna radiata was screened for growth behaviour in acid, saline and alkaline media. It grew well in yeast-extract mannitol broth of wide pH range as well as varying concentrations of NaCl, Na2SO4 and MnCl2. Variation in nodulation and N2-fixation efficiency occurred on different varieties of green gram, while strain characteristics were not affected by soil stress.

scholarly journals Use of extruded hard-to-cook bean flour in substitution of yeast extract and peptone in microbiological culture media

2009 ◽  
Vol 25 ◽  
pp. S232-S233 ◽  
Author(s):  
K.F. Fernandes ◽  
K.A. Batista ◽  
I.T.N. Campos ◽  
L.A. Bataus
Keyword(s):  
Yeast Extract ◽  
Culture Media ◽  
Microbiological Culture ◽  
Bean Flour

Production and characterization of biomass and exopolysaccharides obtained in submerged culture under different initial pHs used in the cultivation of Colletotrichum gloeosporioides and Rhizopus stolonifer

2020 ◽  
pp. 628
Author(s):  
Juan Diego Valenzuela Cobos ◽  
René Oscar Rodríguez-Grimón ◽  
Ana Grijalva-Endara ◽  
Raúl Marcillo-Vallejo ◽  
Onay Adonys Mercader-Camejo
Keyword(s):  
Yeast Extract ◽  
Submerged Culture ◽  
Liquid Culture ◽  
Colletotrichum Gloeosporioides ◽  
Culture Media ◽  
Mycelial Biomass ◽  
Rhizopus Stolonifer

Colletotrichum gloeosporioides (GC003) and Rhizopus stolonifer (RS001) were cultivated in two different liquid culture media: LC1 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 2 g L-1) and LC2 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 10 g L-1) for the production of mycelial biomass and exopolysaccharides (EPS). By using the liquid culture (LC2) under pH of 4.5 presented the highest biomass content (15.73 g L-1) in the propagation of Rhizopus stolonifer. The highest production of exopolysaccharides (1.74 g L-1) was obtained by the liquid culture (LC2) under pH of 4.5 in the cultivation of Colletotrichum gloeosporioides. The results presented that the production of biomass and exopolysaccharides (EPS) is directly related with the pHs values and the strain used in the cultivation.

scholarly journals Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa

2012 ◽  
Vol 17 (1) ◽  
pp. 43 ◽  
Author(s):  
Jimena Paola Angulo-Cortés ◽  
Anamaría García-Díaz ◽  
Aura Marina Pedroza ◽  
María Mercedes Martínez-Salgado ◽  
Viviana Gutiérrez-Romero
Keyword(s):  
Phosphatase Activity ◽  
Microbial Growth ◽  
Culture Media ◽  
Biomass Concentration ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Strains ◽  
Box Behnken Design ◽  
Phosphate Solubilizing ◽  
Complex Culture

<strong>Objective</strong>. To design a complex culture media for the production of biomass and acid phosphatases from phosphate-solubilizing bacteria isolated from soil. <strong>Materials</strong> <strong>and methods</strong>. Phosphate-solubilizing bacteria were isolated from oil palm crop soil samples and selected on SMRS1 agar, which were then assessed with antagonism tests to verify their aptitude to form a co-culture. A Box-Behnken experimental design was applied to<br />evaluate the effect of each one of the culture media components on the production of biomass and phosphatase enzymes at a laboratory scale. Finally, microbial growth and enzyme production curves were carried out in order to determine their production times. <strong>Results</strong>. Five phosphate-solubilizing bacterial strains were isolated and three of them were selected based on their solubilization indices.These Gram negative strains with bacillus morphology were identified as A, B and C; their solubilization indices were 2.03, 2.12, and 2.83, respectively. According to the ANOVA analyses for the Box-Behnken design, the only factor which had a significant effect on the phosphatase activity (p&lt;0.01) was hydrolyzed yeast, and the formulation that generated the highest biomass concentration and phosphatase activity (p&lt;0.01) contained 10, 15 and 2.5 gL-1 of phosphoric rock, sucrose and hydrolyzed yeast, respectively. After 24 hours of incubation at 100 rpm, the highest values of biomass and phosphatase activity were obtained: 11.8 logarithmic units of CFU and 12.9 phosphatase units. <strong>Conclusion</strong>. We determined that the culture media based on phosphoric rock 10 gL-1, hydrolyzed yeast 2.5 gL-1 and commercial sucrose 15 gL-1 was ideal for the production of biomass and phosphatases by the strains evaluated; likewise, we proved that the hydrolyzed yeast was the only factor significantly influential for the production of phosphatases.<br /><br /><strong>Key words</strong>: bio-inoculants, phosphate solubilizing microorganisms, phosphatase activity, Box Behnken design.

scholarly journals Spirulina Culture in Bangladesh XII. Effects of Different Culture Media, Different Culture Vessels and Different Cultural Conditions on Coiled and Straight Filament Characteristics of Spirulina

1970 ◽  
Vol 43 (3) ◽  
pp. 369-376 ◽  
Author(s):  
Parvin Noor ◽  
Nasima Akhtar ◽  
John Liton Munshi ◽  
Sajeda Begum
Keyword(s):  
Key Words ◽  
Pilot Plant ◽  
Culture Media ◽  
Media Culture ◽  
Cultural Condition ◽  
Stock Culture ◽  
Cultural Conditions ◽  
Survival Capacity ◽  
Food Value

Effects of different culture media (Bd-2, Bd-3, Bd-4 and IFP), different culture vessels (conical flasks, plastic bowls and earthen pots) and different cultural conditions (stock, pilot plant and shade culture) on coiled and straight filament characteristics of Spirulina were studied. Stock culture maintained under special conditions, retained coiled filament characteristics even after 17 years, while Spirulina filaments in pilot ponds lost their coiled characteristics. Food value of the straight filaments was acceptable and their survival capacity was more than that of coiled filaments. Key words: Spirulina, Media culture, Vessels culture, Cultural condition, Filaments character.  DOI = 10.3329/bjsir.v43i3.1152Bangladesh J. Sci. Ind. Res. 43(3), 369- 376, 2008

Simultaneous Growth of Listeria monocytogenes and Listeria innocua

1993 ◽  
Vol 56 (7) ◽  
pp. 616-618 ◽  
Author(s):  
RUTH L. PETRAN ◽  
KATHERINE M. J. SWANSON
Keyword(s):  
Listeria Monocytogenes ◽  
Yeast Extract ◽  
Environmental Samples ◽  
Culture Media ◽  
Listeria Innocua ◽  
Enrichment Broth ◽  
University Of Vermont ◽  
Simultaneous Growth

Listeria spp. have been isolated from a wide variety of sources, and in many situations Listeria innocua is more commonly found than Listeria monocytogenes. Growth of three L. monocytogenes strains was studied when inoculated simultaneously with a rhamnose negative L. innocua strain into culture media and cheese sauce. Fraser broth (FB), Trypticase™ soy broth plus 0.6% yeast extract (TSB-YE), University of Vermont medium (UVM) modified Listeria enrichment broth, and cheese sauce were inoculated (ca. 102 cells per ml) and incubated for 24 h; FB, TSB-YE, and cheese sauce at 35°C, UVM at 30°C. Growth of four rhamnose-positive, L. innocua strains was also studied in culture media. Growth of L. monocytogenes was similar to that for L. innocua in TSB-YE or cheese sauce. However, in FB and UVM, L. innocua populations were significantly higher than L. monocytogenes. This occurred when media were inoculated individually or simultaneously. This may explain in part why L. innocua is isolated more frequently than L. monocytogenes from foods and environmental samples.

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