scholarly journals Effects of Liquid Digestate Treatment on Sustainable Microalgae Biomass Production

2021 ◽  
Author(s):  
Marta Kisielewska ◽  
Marcin Dębowski ◽  
Marcin Zieliński ◽  
Joanna Kazimierowicz ◽  
Piera Quattrocelli ◽  
...  
Keyword(s):  
Growth Medium ◽  
Biomass Production ◽  
Culture Medium ◽  
Culture Media ◽  
Biomass Concentration ◽  
Arthrospira Platensis ◽  
Microalgal Biomass ◽  
Liquid Digestate ◽  
Microalgae Biomass ◽  
High Treatment

AbstractThe aim of the study was to investigate the potential of microalgal cultivation on anaerobic liquid digestate as a growth medium. The two methods of liquid digestate treatment including centrifugation and distillation and the two algal strains (Chlorella vulgaris and Arthrospira platensis) were compared. Additionally, the volume of the liquid digestate used to prepare the culture medium constituted from 10 to 50% of the medium volume. The study demonstrated that the highest C. vulgaris and A. platensis biomass productions of 2490 mg TS/L and 2990 mg/L, respectively, were obtained by adding 50% of distilled digestate to a growth medium. Regarding centrifuged liquid digestate, only 10% dilution was required to obtain the maximum final biomass concentration. A. platensis removed 81.1% and 66.4% of the total nitrogen from medium prepared on distilled and centrifuged digestate, respectively, while C. vulgaris ensured 64.1% and 47.1% of removal, respectively. The phosphorus removal from both culture media was higher than 94.2% with A. platensis, while it was 70.4% from distilled and 87.4% from centrifuged media with C. vulgaris. The study confirmed a great potential of microalgal biomass production on anaerobic liquid digestate with a high treatment efficiency of digestate.

scholarly journals Sustainable Production of Monoraphidium Microalgae Biomass as a Source of Bioenergy

Energies ◽  
2020 ◽  
Vol 13 (22) ◽  
pp. 5975
Author(s):  
Małgorzata Hawrot-Paw ◽  
Adam Koniuszy ◽  
Małgorzata Gałczyńska
Keyword(s):  
Phosphorus Content ◽  
Biomass Concentration ◽  
Biomass Productivity ◽  
Ash Content ◽  
Saline Wastewater ◽  
Microalgal Biomass ◽  
Recirculating Aquaculture ◽  
Microalgae Biomass ◽  
The Individual ◽  
Tubular Photobioreactors

Microalgae are a renewable source of unconventional biomass with potential application in the production of various biofuels. The production of carbon-neutral fuels is necessary for protecting the environment. This work determined the possibility of producing biomass of microalgae belonging to Monoraphidium genus using saline wastewater resulting from proecological salmon farming in the recirculating aquaculture system. The tests were carried out in tubular photobioreactors using LED light. As a part of the analyses, the growth and productivity of microalgal biomass, cell density in culture, and lipid concentration and ash content in biomass were determined. In addition, the concentration of selected phosphorus and nitrogen forms present in wastewater corresponding to the degree of their use by microalgae as a nutrient substrate was determined. The biomass concentration estimated in the tests was 3.79 g·L−1, while the maximum biomass productivity was 0.46 g·L−1·d−1. The cells’ optical density in culture measured at 680 nm was 0.648. The lipid content in biomass was 18.53% (dry basis), and the ash content was 32.34%. It was found that microalgae of the genus Monoraphidium effectively used the nitrogen as well as phosphorus forms present in the wastewater for their growth. The total nitrogen content in the sewage decreased by 82.62%, and total phosphorus content by over 99%. The analysis of the individual forms of nitrogen showed that N-NO3 was reduced by 85.37% and N-NO2 by 78.43%, while orthophosphate (V) dissolved in water was reduced by 99%. However, the content of N-NH4 in wastewater from the beginning till the end of the experiment remained <0.05 mg·L−1.

Metal-metabolomics of microalga Chlorella sorokiniana growing in selenium- and iodine-enriched media

2012 ◽  
Vol 66 (9) ◽  
Author(s):  
Veronica Gómez-Jacinto ◽  
Tamara García-Barrera ◽  
Ines Garbayo-Nores ◽  
Carlos Vilchez-Lobato ◽  
José-Luis Gómez-Ariza
Keyword(s):  
Molecular Mass ◽  
Inductively Coupled Plasma ◽  
Culture Medium ◽  
Culture Media ◽  
Sodium Dodecyl ◽  
Biomass Concentration ◽  
Chlorella Sorokiniana ◽  
Hot Water ◽  
Size Exclusion ◽  
Icp Ms

AbstractThe microalga Chlorella sorokiniana has been used to accumulate selenium and iodine from culture media enriched with these elements as a first stage in the production of supplemented foods. The microalgal colony was grown in a conventional culture medium containing iodine (KI) at concentrations in the range of 150–4000 μg mL−1. Similar experiments were performed with selenium (SeO42−) at concentrations in the range of 20–500 μg mL−1. The concentration of iodine and selenium in the culture medium was analytically monitored daily and the viability of the colony was checked by biomass concentration measurement and by evaluation of the total content of chlorophyll and carotenoids. In addition, photosynthetic activity and the number of cells were also monitored. Iodine accumulation in the algal biomass increased rapidly with time and reached a steady state after 4 h of exposure. With Se exposure the colony viability decreased, although the culture grew well with concentrations of the element of 50 μg mL−1 in the culture medium; this experiment produced Se-enrichment in the alga (3 μg g−1) within 100 h. Sequential extraction of an algal pellet was performed in order to separate Se compounds according to their affinity with the following solvents: hot water to recover low molecular mass Se species, enzymatic extraction with driselase for species associated with the cell wall, sodium dodecyl sulphate (SDS) for water insoluble selenoproteins and, finally, enzymolysis with lipase and pronase that release and fragment residual selenoproteinsproducing compounds with low molecular mass. Size-exclusion chromatography (SEC) coupled with an ICP-MS detector showed the preponderance of Se-containing molecules with low molecular mass, possibly seleno-amino acids. Only a peak of low intensity located at 10 min was observed in the SDS extract that could be associated with a protein with molecular mass of 67 kDa. Finally, analysis of the aqueous extract of alga by reverse-phase chromatography with inductively-coupled plasma mass-spectrometry (RPC-ICP-MS) detection revealed the presence of selenocysteine (SeCys2), selenomethylselenocysteine (SeMetSeCys), selenomethionine (SeMet), and Se(VI), particularly the last two species.

Modified equation of chlorophyll content in microalgal biomass under light limitation

2020 ◽  
pp. 17-24
Author(s):  
Alexander S. Lelekov ◽  
Rudolf P. Trenkenshu ◽  
Tatyana M. Novikova
Keyword(s):  
Chlorophyll Concentration ◽  
Arthrospira Platensis ◽  
Light Limitation ◽  
Specific Rate ◽  
Microalgal Biomass ◽  
Basic Model ◽  
Microalgae Biomass ◽  
Rate Of Photosynthesis ◽  
Species Specific ◽  
Irradiation Parameter

The paper presents a modified mathematical model of light-dependent chlorophyll concentration in the microalgae biomass. The basic model is based on the concept of biomass as a sum of reserve and structural components. Considering that part of the structural biomass can turn into a reserve, the minimum irradiation parameter (“photosynthesis compensation point”) is added to the basic equation. Verification of the model on experimental data for turbidostat cultures of Tetraselmis viridis and Arthrospira platensis allowed us to evaluate species-specific coefficients. The obtained coefficient values are similar for both species. The share of chlorophyll in structural biomass is about 2 %, the maximum share of structural forms of biomass is 84 %, the economic conversion factor of reserve biomass to structural is 80–90 %, and the maximum specific rate of photosynthesis is 14–20 times higher than the rate of endogenous consumption of biomass.

Optimization of an effective growth medium for culturing probiotic bacteria for applications in strict vegetarian food products

2012 ◽  
Vol 2 (10) ◽  
pp. 369 ◽  
Author(s):  
Manju Pathak ◽  
Danik Martirosyan
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Growth Medium ◽  
Culture Medium ◽  
Culture Media ◽  
Plant Seed ◽  
Meat Extract ◽  
Colony Forming Units ◽  
Lactobacillus Lactis ◽  
De Man

Background: This study aimed to modify de Man Rogosa Sharpe culture medium (termed MRS) for selective cultivation of probiotics strain for the consumption by the strictly vegetarian human population. Vegetarian probiotic foods by definition must be free from all animal-derived ingredients. This not only includes the product ingredients but the probiotic inoculum as well. Probiotic starter cultures are traditionally grown and stored in media containing milk or meat-derived ingredients. The presence of these ingredients makes the probiotic cell concentrates unsuitable for use in vegetarian products and thus creates the need for a growth medium which is free from animal-derived ingredients. Present study investigated the growth of a strain of Lactobacillus lactis in MRS. The present invention relates in general to a bacterial culture media, and more specifically a complex microbial culture media, based on plant seed powder extract in place of animal extract for probiotic bacterial growth.Methods: Lactobacillus lactis, a probiotic, was grown in standard MRS culture medium as well as in our various test media (TM) containing various vegetal source in place of beef extract, yeast extract and peptone as in case of MRS. The inoculated culture mediums were incubated at 37C for 72 hours and growth of probiotic is recorded at regular intervals. The growth was recorded as Colony Forming Units (CFUs).Results: The best growth of probiotic is observed in TM 2. TM 2 is the leguminous seed extract. Starter culture mediums for probiotics or other bacteria primarily contain protein from animal source. The possibility of using vegetal protein from TM 2 extract in place of peptones and meat extract for the nitrogen supplementation of culture media for the growth of lactic acid bacteria has been demonstrated. Conclusion: The absolute vegetarian culture medium containing TM 2 is better than standard MRS for the growth of probiotics.Abbreviations: de Man Rogosa Sharpe (MRS), Colony Forming Units (CFU), test media (TM), National Dairy Research Institute (NDRI), Tamarind seed powder (TSP), solid-state fermentation (SSF), Lactobacillus casei Shirota (LcS)Keywords: probiotics, lactic acid bacteria, vegetarian

scholarly journals Effectiveness of Scenedesmus sp. Biomass Grow and Nutrients Removal from Liquid Phase of Digestates

Energies ◽  
2020 ◽  
Vol 13 (6) ◽  
pp. 1432 ◽  
Author(s):  
Marta Kisielewska ◽  
Marcin Zieliński ◽  
Marcin Dębowski ◽  
Joanna Kazimierowicz ◽  
Zdzisława Romanowska-Duda ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Organic Compounds ◽  
Growth Medium ◽  
Ammonia Nitrogen ◽  
Phosphorus Concentration ◽  
Limiting Factor ◽  
Cultivation Medium ◽  
Initial Concentration ◽  
Liquid Digestate ◽  
Microalgae Biomass

One of the most important factors in determining the profitable production of microalgae biomass is the use of a cost effective growth medium that is rich in nutrients. The objective of the study was to determine the possibility of using digestates from anaerobic digestion of different feedstock mixtures as the media for Scenedesmus sp. cultivation. A different liquid digestate composition was obtained in terms of organic compounds, phosphorus, and nitrogen concentrations, depending on the substrates used in the anaerobic digestion. It was found that the highest biomass production was obtained when using digestate from anaerobic digestion of the feedstock mainly composed of microalgae biomass, which was characterized by low organic compounds concentration. In this case, the average biomass concentration reached 2382 mg total solids/L. A lower Scenedesmus sp. biomass yield was obtained using digestate from anaerobic digester processing feedstock based on maize silage and cattle menure. In the variants of the study, it was also found that the increase in the initial concentration of ammonia nitrogen in the growth medium up to 160 mg/L significantly reduced the growth of Scenedesmus sp. The results indicated the possibility of a high ammonia nitrogen and orthophosphates removal from anaerobic digestates by Scenedesmus sp. microalgae. Phosphorus concentration in the cultivation medium is a limiting factor for the growth of Scenedesmus sp., thus phosphorus supplementation should be considered when using liquid digestate as the culture medium. The optimization model indicated that the volume of liquid digestate that was used for preparing the cultivation medium, the initial concentration of organic compounds, and the initial concentration of ammonia nitrogen had a significant impact on the production of Scenedesmus sp. biomass.

scholarly journals The Cultivation of Lipid-Rich Microalgae Biomass as Anaerobic Digestate Valorization Technology—A Pilot-Scale Study

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 517 ◽  
Author(s):  
Marcin Dębowski ◽  
Marcin Zieliński ◽  
Marta Kisielewska ◽  
Joanna Kazimierowicz ◽  
Magda Dudek ◽  
...  
Keyword(s):  
Culture Medium ◽  
Biomass Concentration ◽  
Pilot Scale ◽  
Dairy Wastewater ◽  
Small Scale ◽  
Treatment Efficiency ◽  
Average Growth Rate ◽  
Average Growth ◽  
Bio Oil ◽  
Microalgae Biomass

The aim of the study was to determine the use of digestate from anaerobic digestion of dairy wastewater as a culture medium for microalgae to obtain bio-oil. The experiments were conducted at a small scale in a closed raceway pond. The efficiency of the microalgae biomass production, the digestate treatment efficiency as well as the content and properties of the bio-oil obtained from the microalgal cells were analyzed. The produced biomass concentration was about 3000 ± 10.5 mg dry biomass/L, with an average growth rate of 160 ± 6.6 mgdm/L·d. The efficiency of organic compound and nutrient removal was above 90%. The bio-oil content in the biomass was about 20%. Based on the results of the study, a concept for technical-scale technology was developed.

scholarly journals Characterization of Arthrospira platensis cultured in wastewater of Clarias catfish farming media: DNA barcode, helical form, growth, and phycocyanin

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Marini Wijayanti ◽  
Mochamad Syaifudin ◽  
Yulisman Yulisman ◽  
Yully Nurianti ◽  
Anita Hidayani ◽  
...  
Keyword(s):  
Growth Rate ◽  
Phylogenetic Trees ◽  
Culture Medium ◽  
Culture Media ◽  
Dna Barcode ◽  
Arthrospira Platensis ◽  
Rrna Gene ◽  
Randomized Design ◽  
Density Growth

Abstract. Wijayanti M, Syafudin M, Yulisman, Nurianti Y, Hidayani A, Gofar N. 2020. Characterization of Arthrospira platensis cultured in wastewater of Clarias catfish farming media: DNA barcode, helical form, growth, and phycocyanin. Biodiversitas 21: 5872-5883. Arthrospira production technology in catfish waste media can be an alternative to reduce environmental pollution. However, some environmental factors such as nutrition, light, and water content can influence characterization of Arthrospira at the genetic and physiologic level. Arthrospira platensis is one of the phycocyanin-producing cyanobacteria and can be cultured using catfish culture wastewater. Water quality especially pH and salinity can effect of growth rate and residue of phycocyanin from Arthrospira platensis. This study aimed to identify the species and morphological forms of Arthrospira cultured using technical fertilizer and waste media, as well as to know the phylogenetic trees between species in this study and the GeneBank based on the 16S rRNA gene, and determine the optimum of pH and salinity required in the medium of catfish culture wastewater to phycocyanin maximum production of Arthrospira. The optimation of pH and salinity method used Completely Randomized Design (CRD) factorial with 2 factors consisting of the first factor with 3 treatments and the second factor with 4 treatments and 3 replications. The first factor was pH of culture medium i.e. pH 6.5 ± 0.2, pH 8.5 ± 0.2 and pH 10.5 ± 0.2. The second factor was salinity of culture medium, that was salinity 0 ppt (parts per thousand/‰), 10 ppt, 20 ppt, and 30 ppt. Parameters observed in Arthrospira include density, growth rate, rendement of phycocyanin, and decreased total nitrogen and phosphate content in culture media. The results showed that morphology Arthrospira cultured on technical fertilizer media (AF) had a longer and helix filament compared to Arthrospira cultured on waste media (AW) which showed several linear and shorter filaments. Both samples have a genetic distance of 0.068 (6.8%). Phylogenetic trees indicated that AF had a close relationship with Arthrospira platensis petH from Japan (bootstrap value 95%). While AW formed a separate sub-cluster of AF isolates and Arthrospira platensis petH from Japan (bootstrap value of 85%). The best treatment in this study was P2S3 (pH 8.5 ± 0.2 with salinity 20 ppt), which produced 0.867 grams maximum density, growth rate of 22.026 %.day-1 and 11.347 mg.g1 rendement of phycocyanin.

Sign in / Sign up

Export Citation Format

Share Document