scholarly journals Urban secondary sewage: an alternative medium for the culture of Tetraselmis chuii (Prasinophyceae) and Dunaliella viridis (Chlorophyceae)

2004 ◽  
Vol 47 (3) ◽  
pp. 451-459 ◽  
Author(s):  
Rauquírio André Albuquerque Marinho da Costa ◽  
Maria Luise Koening ◽  
Sílvio José de Macedo
Keyword(s):  
Culture Media ◽  
Chicken Manure ◽  
Dunaliella Viridis ◽  
Chi Square ◽  
Nutritive Medium ◽  
Tetraselmis Chuii ◽  
Alternative Culture ◽  
Chi Square Test ◽  
Cell Densities ◽  
Intensive Aquaculture

The effect of different concentrations (10, 20, 30 and 40%) of urban secondary sewage on the growth of Tetraselmis chuii (Prasinophyceae) and Dunaliella viridis (Chlorophyceae) was examined to verify the possibility of its use as an alternative culture medium for these species. Stocks and 700mL duplicate batch cultures were maintained under controlled laboratory conditions. 30% and 40% additions were the most efficient for the growth of both species. Statistical analysis of the adjusted growth (cell.mL-1) and biomass (µg chlorophyll-a.mL-1) curves performed using the Chi-square test (p<0.05) demonstrated significant differences between the nutritive medium f/2 and the 40% addition used for these two species. The results suggested that some of the concentrations tested here yielded satisfactory cell densities and growth rates when compared with other culture media (macroalgae, bovine and chicken manure extracts). Thus it could be used by semi intensive aquaculture systems.

137 EFFECT OF THE ADDITION OF FOLIC ACID TO MATURATION AND CULTURE MEDIA ON DEVELOPMENT OF THE BOVINE BLASTOCYST AND ITS SURVIVAL RATE AFTER FREEZE-THAWING

2017 ◽  
Vol 29 (1) ◽  
pp. 177
Author(s):  
S. Sato ◽  
O. Dochi ◽  
K. Imai
Keyword(s):  
Folic Acid ◽  
Survival Rate ◽  
Culture Media ◽  
Cell Damage ◽  
Calf Serum ◽  
Cleavage Rate ◽  
Chi Square ◽  
Exact Test ◽  
Chi Square Test

Reactive oxygen species (ROS) are the main causes of cell damage in bovine embryos in vitro. Folic acid (FA) is an antioxidant that protects cells from ROS. We studied the effect of the addition of FA to maturation and culture media on development of bovine blastocysts and their survival rate after freeze-thawing. Cell-oocyte complexes (COC) were allowed to mature in HEPES (25 mM)-buffered TCM199 (TCM199) supplemented with 5% calf serum (CS), 0.02 AU mL−1 of FSH, and FA (0, 2.5, 25, and 50 µM) for 20 hours (20–25 COC/100-µL droplet of the medium). After 6 hours of gamete co-culture (5 × 106 sperm/mL), presumptive zygotes were cultured in CR1aa medium supplemented with 5% CS and FA (0, 2.5, 25, and 50 μM) for 9 days (day of fertilization = Day 0). Expanded blastocysts that developed from Day 7 to 9 were frozen for further study. Each embryo was frozen in Dulbecco’s PBS (D-PBS) supplemented with 20% CS, 1.5 M ethylene glycol (EG), and 0.1 M sucrose (SUC). Embryos were equilibrated with their freezing medium for 15 min and loaded individually into a 0.25-mL straw. These straws were put into the cooling chamber of a programmable freezer precooled at −7°C. After 2 min, straws were seeded and held for 13 min at −7°C. Next, straws were cooled to −30°C at −0.3°C/min before being plunged into liquid nitrogen. Frozen embryos were thawed by allowing straws to stand in air for 7 s and warming them in a 30°C water bath for 20 s. Thawed embryos were washed twice with D-PBS supplemented with 20% fetal calf serum (FCS), which was warmed to 38°C. They were immersed into the same medium at 38°C for 10 min, and each embryo was cultured in a 20-μL droplet of TCM199 supplemented with 10% FCS and 0.1 mM β-mercaptoethanol (TCM-199-βME) for 72 h. Embryo cleavage rate was observed at 55 h post-insemination. Blastocyst rates were analysed at 9 days post-insemination. Rates of embryos developing into reexpanded, hatching, and hatched blastocyst stages were determined after 72 h of thawing. All data were analysed by the chi-square test and Fisher’s exact test. Cleavage and blastocyst rates after insemination at 55 hours and 9 days, respectively, were not significantly different among media containing 0 μM (n = 278; 74.1% and 39.9%), 2.5 μM (n = 260; 74.2% and 45.8%), 25 μM (n = 258; 75.6% and 45.7%), and 50 μM (n = 253; 76.3% and 42.7%) FA. Survival and hatching rates of frozen and thawed expanded blastocysts after 72 h in culture were 62.5% and 56.3%, respectively, in 0 μM FA (n = 16); 85.2% and 74.1% in 2.5 μM FA (n = 27); 66.7% and 62.5% in 25 μM FA (n = 24); and 68.0% and 64.0% in 50 μM FA (n = 25). Blastocysts cultured in media containing 2.5 μM FA tended to have a higher survival rate than those cultured in media containing 0 μM FA, although this difference was not significant (P = 0.09). Inclusion of FA did not appear to influence development or post-thaw survival of bovine blastocysts produced in vitro.

125 A COMPARISON OF SEQUENTIAL MEDIUM WITH NON-SEQUENTIAL MEDIUM: DO SOME PATIENTS' EMBRYOS CULTURE BETTER IN ONE THAN IN THE OTHER?

2009 ◽  
Vol 21 (1) ◽  
pp. 162 ◽  
Author(s):  
K. O. Pomeroy ◽  
S. Foley ◽  
B. Faber ◽  
D. V. Moffitt ◽  
M. D. Johnson
Keyword(s):  
Culture Media ◽  
Pregnancy Rates ◽  
The Other ◽  
Test Results ◽  
Chi Square ◽  
Treatment Development ◽  
Chi Square Test ◽  
Split Treatment ◽  
Implantation Rates

The development of sequential media is often reported as one of the reasons why human IVF pregnancy rates have increased over the last 10 years. The objective was to determine if a single culture medium (global) is as effective in human embryo culture as a sequential medium (G series) and whether embryos of some patients prefer one medium over the other. Patients (n = 144) were placed into one of three treatment arms. Treatment 1, sequential: culture in G Series medium. Treatment 2, nonsequential: culture in global medium. Treatment 3, split: After fertilization, a patient’s embryos are split between the above two treatments. All patients with embryos from April 2007 to January of 2008 (PGD patients were excluded) were allocated to one of the three treatments. Eggs were collected in and fertilized in vitro Life media (GMOPS and GFert, respectively). In treatment 1, patients’ embryos (n = 346) were cultured in sequential media. In treatment 2, patients’ embryos (n = 482) are cultured in nonsequential media. In treatment 3, embryos (n = 420) from each patient are split between sequential and nonsequential media after rinsing in the treatment medium. Patients whose embryos met the criteria (at least 2 embryos, >6 cells, grade excellent to good) were transferred on day 5. All embryos remaining after embryo transfer were frozen on day 5. A maximum of 2 embryos was transferred per patient. Frequencies were compared with a chi square test. Results: The percentage of cases that achieved transfers on Day 5 (an indication of embryo quality) were 44.0%, 50.0% and 68.4% for sequential, nonsequential and split, respectively. Split was significantly different from the sequential (P < 0.05). The percentage of transferrable blastocysts was 27% for sequential, 43% for nonsequential and 39% for split (difference between sequential and the other two was significant at P < 0.0005). Pregnancy rates and implantation rates were 32%/19.0%, 36%/22.5% and 50%/32.2% for sequential, nonsequential and split. (Only implantation rates between split and sequential were significant P < 0.05). For embryos in the split treatment, development to transferable blastocysts was 37.0% in sequential media v. 41.6% in nonsequential medium (not significant). In conclusion, a single medium (nonsequential) for culture to blastocyst yields results similar to embryos cultured in the more complex sequential medium. There is some evidence that a benefit can be gained by splitting embryos between two culture media to increase implantation rates. Further studies need to be done to see if this will result in increased pregnancy rates.

scholarly journals DESIGN AND ANALYSIS OF COCONUT EMBRYO CULTURE EXPERIMENTS

CORD ◽  
2003 ◽  
Vol 19 (01) ◽  
pp. 34
Author(s):  
Anitha Karun ◽  
K. Muralidharan ◽  
K. K. Sajini ◽  
V.A. Parthasarathy
Keyword(s):  
Embryo Culture ◽  
Culture Media ◽  
Zygotic Embryo ◽  
Experimental Unit ◽  
Chi Square ◽  
Planning Stage ◽  
Zygotic Embryo Culture ◽  
Plot Size ◽  
Chi Square Test ◽  
Experimental Errors

The experimental design and data analysis procedures followed for standardizing the CPCRI protocol of coconut zygotic embryo culture are described. The availability of human resources (for initiating the cultures as well as periodic sub-culturing), laboratory space for maintaining culture, infrastructure for preparation of adequate culture media etc, are the important factors to be considered at the planning stage. Since the response of interest per experimental unit is discrete (germinated or not), it is desired to have a “plot size” of 20. Based on the experimental errors obtained for various treatments, attempts were made to obtain the replication size for a specified value of treatment difference to be tested. In most of the experiments, per cent germination was not following the assumption of ANOVA , even after transformation. In such situation, the chi-square test was employed to study the influence of treatments on germination. The coefficient of variation in most of the experiments was below 20 %.

Hubungan Antara Menyusui Dengan Involusi Uterus Pada Ibu Nifas Fisiologis Di RSIA Aura Syifa Kabupaten Kediri

1970 ◽  
Vol 5 (2) ◽  
pp. 64
Author(s):  
Eny Sendra ◽  
Dewi Indriani
Keyword(s):  
Breast Feeding ◽  
Sampling Technique ◽  
Chi Square ◽  
Cross Sectional ◽  
Fisher Test ◽  
Key Word ◽  
Chi Square Test ◽  
The Moment

Breast feeding is giving milk to be drunk to the baby from the breast. Uterus involution is a process how the uterus return to the condition back, before pregnanting after bearing. At the moment of suckling, happens a stimulus and brings the hormones out, such as oksitosin uses not only to stimulate some muscles constraction but also to stimulate the uterus, so that the process of uterus involution happens foster. According to the explanations above, the research aimed to know about the correlation between breast feeding and uterus involution. This research’s design was, cross sectional by the population of all childbirth mothers approximately 50 persons / month. By using accidental sampling technique we got 21 sample respondents. The place of research in RSIA Aura Syifa in Kediri Regency on 16th until 22nd of June 2009. From this research’s result, we got 14 persons (66,67%) with normal uterus involution, suckled in a good way, one person (4,67%) with normal uterus involution, suckled in a wrong way, 2 persons (9,52%) with abnormal uterus involution, suckled in a good way and 4 persons (19,05%) with abnormal uterus involution, suckled in a wrong way. Statistic test which used chi-square test, counted the probability frequency in advance, from that we got 3 columns with the score, less than 5, so that chi-square can not be continued and by doing exact fisher test, the score was 0,001. Because P with the grade mistake 0,05 smaller, so the conclusion was “Ho” is rejected, it meant “there was correlation between suckling and uterus involution”. Key Word : Breast feeding, uterus involution

FAKTOR PERSONAL YANG BERHUBUNGAN DENGAN KOMITMEN ORGANISASI PERAWAT RSUD EMBUNG FATIMAH BATAM

2020 ◽  
Vol 5 (3) ◽  
pp. 875
Author(s):  
Asfri Sri Rahmadeni
Keyword(s):  
Organizational Commitment ◽  
Turnover Rate ◽  
Sampling Technique ◽  
Hospital Nurses ◽  
Test Results ◽  
Personal Factors ◽  
High Turnover ◽  
Chi Square ◽  
Cross Sectional ◽  
Chi Square Test

<p class="Normal1"><em>Commitment is the attitude or behavior of likes or dislikes shown by someone against the organization at work. Indonesia is recorded as a nurse who has a low organizational commitment (76%) so it harms services. This phenomenon in the hospital can be seen from the indicator of the high turnover rate of nurses which reaches 30.9% which exceeds the standard &lt;5%. Many factors affect organizational commitment. The purpose of this study was to analyze the Personal Factors Associated with Organizational Commitment of Hospital Nurses. This study is a cross-sectional analytic study and purposive sampling technique with a sample of 59 nurses. Chi-Square test results obtained personal factors that have a relationship with organizational commitment Nurse Hospital is age with p = 0,000 and education with p = 0,000, personal factors not related to organizational commitment are gender p = 1,053 and years of service with p = 1,147. Hospitals should conduct research/surveys on Organizational Commitment periodically as an evaluation material in the context of the organization's development and development in the future</em>.</p>

GAMBARAN INDEKS LARVA AEDES AEGYPTI DI BUFFER WILAYAH KERJA BANDARA SEPINGGAN BALIKPAPAN

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Abdurrakhman abdurrakhman Abdurrakhman
Keyword(s):  
Aedes Aegypti ◽  
Buffer Zone ◽  
Chi Square ◽  
Cross Sectional ◽  
Breeding Sites ◽  
Dengue Disease ◽  
Vector Borne Diseases ◽  
Transportation Equipment ◽  
Chi Square Test ◽  
Larvae Density

ABSTRACT : The House index and Container Index in the buffer area of ​​the working area of ​​Balikpapan Sepinggan Airport is still above 1%, so the potential for the spread of dengue disease. Mobilization of people, goods and transportation equipment will increasingly affect the transmission of disease in ports and airports, especially for vector-borne diseases. This study aims to analyze the risk factors associated with larvae density of Aedes aegypti and describe the larvae index in the buffer zone of the Sepinggan Balikpapan Airport This study was a descriptive study with a cross sectional design. The sample in this study was 121 houses with a proportionate stratified random sampling, the research location was in the buffer zone of Sepinggan Balikpapan Airport in November 2018. The variables studied were houses with positive larvae containers, breeding sites and PSN behavior and larvasidation. The data was analyzed using the chi square test. There was a relationship between houses with larvae positive Aedes aegypti, behavior of Mosquito Nest Eradication (PSN) and larvasidation with larvae density of Aedes aegypti but not for breeding sites (p = 0.00 and 95% CI = 0.64), and   (p = 0.00 and 95% CI = 0.34). The description of several Aedes aegypti larvae index, namely House Index (HI) = 57.02%, Container Index (CI) = 24.36%, Bruteau Index (BI) = 148.76, and Flick Free Numbers (ABJ) = 42.98 %. Houses with larvae of Aedes aegypti larvae and PSN and larvasidation behavior were associated with larvae density of Aedes aegypti. The index of HI, CI and BI larvae is of high value so there is a risk of DBD transmission

The Influence of Age and Gender on Mandibular Indices among the Libyan Population

2019 ◽  
Vol 3 (2) ◽  
Keyword(s):  
Negative Correlation ◽  
Bone Mineralization ◽  
Age Groups ◽  
T Test ◽  
Chi Square ◽  
Age And Gender ◽  
Significant Difference ◽  
Chi Square Test ◽  
And Gender ◽  
Libyan Population

Radiographic Mandibular Indices serve as easy and relatively cheap tools for evaluating bone mineralization. Objectives: To examine the effect of age and gender on three mandibular indices: the panoramic mandibular index (PMI), the mandibular ratio (MR) and the mandibular cortical index (MCI), among Libyan population. Methods: The three indices were measured on 317 digital (OPGs) of adult humans (155 males, 162 females). The sample was divided into six age groups (from 18-25 years through 56-65 years). The measurements were analyzed for interactions with age and sex, using SPSS (Statistical Package for Social Studies) software version no. 22. The tests employed were two way ANOVA, the unpaired T-test and chi-square test. Results: The mean PMI fluctuated between 0.37 s.d. 0.012 and 0.38 s.d. 0.012. among the sixth age groups. One-way ANOVA statistical test revealed no significant of age on PMI. On the other hand gender variation has effect on PMI, since independent sample t-test disclosed that the difference between the male and female PMI means statistically significant. ANOVA test showed that the means of MR among age groups showed a negative correlation i.e. MR mean declined from 3.01 in 18-25 age groups to 2.7 in 55-65 age groups. In contrary, the gender showed no effect on MR according two sample t-test at p> 0.05. In regards with MCI, statistical analysis showed that it affected by age that is C1 was decreasing by age while C2 and C3 were increased by age. Using chi square test the result indicated that there is a significant difference among the different age group and the two genders in MCI readings. Conclusion: PMI was influenced significantly by age but minimally by the gender. MR is not affected by gender but has a negative correlation with age. MCI is affected by both age and gender

IDENTIFIKASI SERTA HUBUNGAN KONSTRUKSI SUMUR GALI TERHADAP KANDUNGAN COLIFORM PADA AIR SUMUR GALI DI DUSUN JIKEN KABUPATEN LOMBOK TIMUR

2019 ◽  
Vol 7 (2) ◽  
pp. 115
Author(s):  
Dini Yuliansari
Keyword(s):  
Cross Sectional Study ◽  
Coliform Bacteria ◽  
Well Water ◽  
Chi Square ◽  
Cross Sectional ◽  
Content Variable ◽  
Chi Square Test ◽  
Dug Wells ◽  
Dug Well ◽  
The Relationship

The well is one of the source water used for bathing, washing, and drinking by the citizens in Jiken Hamlet, Rarang Village, Terara Sub-District. Poor dug well construction conditions can affect the amount of coliform bacteria contained in the dug well water. The purpose of this study is to identify the construction of dug wells and the content of coliform bacteria in dug well water and determine the relationship between dug well construction and the content of coliform bacteria. This research is analytic with a cross sectional study design. The results were obtained from 11 samples of dug wells which were observed in construction and the coliform content was known that all samples did not requirements as good dug well construction. Coliform content test results showed that as many as 5 dug well water samples did not pass the clean water quality standards. The results of data analysis with the chi-square test variable construction of dug wells with coliform content variable  showed 0,172 > 0,05, then the concluded is the dug well not suitable to use by people in that area as a source of water for daily needs.

Hubungan antara Luas Lesi pada Foto Toraks Penderita TB Paru Dewasa yang Memiliki Riwayat Diabetes Melitus dengan Indeks Massa Tubuh di Rumah Sakit Hasan Sadikin Bandung

2016 ◽  
Vol 1 (3) ◽  
pp. 138-144
Author(s):  
Ina Edwina ◽  
Rista D Soetikno ◽  
Irma H Hikmat
Keyword(s):  
Chest Radiograph ◽  
Chi Square ◽  
Pulmonary Tb ◽  
X Ray ◽  
Consecutive Sampling ◽  
Close Relationship ◽  
Chi Square Test ◽  
Chest X Ray ◽  
The Relationship ◽  
Diabetes Melitus

Background: Tuberculosis (TB) and diabetes mellitus (DM) prevalence rates are increasing rapidly, especially in developing countries like Indonesia. There is a relationship between TB and DM that are very prominent, which is the prevalence of pulmonary TB with DM increased by 20 times compared with pulmonary TB without diabetes. Chest X-ray picture of TB patients with DM is atypical lesion. However, there are contradictories of pulmonary TB lesion on chest radiograph of DM patients. Nutritional status has a close relationship with the morbidity of DM, as well as TB.Objectives: The purpose of this study was to determine the relationship between the lesions of TB on the chest radiograph of patients who su?er from DM with their Body Mass Index (BMI) in Hasan Sadikin Hospital Bandung.Material and Methods: The study was conducted in Department of Radiology RSHS Bandung between October 2014 - February 2015. We did a consecutive sampling of chest radiograph and IMT of DM patients with clinical diagnosis of TB, then the data was analysed by Chi Square test to determine the relationship between degree of lesions on chest radiograph of pulmonary TB on patients who have DM with their BMI.Results: The results showed that adult patients with active pulmonary TB with DM mostly in the range of age 51-70 years old, equal to 62.22%, with the highest gender in men, equal to 60%. Chest radiograph of TB in patients with DM are mostly seen in people who are obese, which is 40% and the vast majority of lesions are minimal lesions that is equal to 40%.Conclusions: There is a signifcant association between pulmonary TB lesion degree with BMI, with p = 0.03

Analisis Faktor Determinan Pelaksanaan Inisiasi Menyusu Dini Oleh Bidan Di Rumah Sakit Umum Anutapura Palu

2018 ◽  
Vol 1 (1) ◽  
pp. 34
Author(s):  
Ni Nyoman Novita ◽  
Gusman Arsyad
Keyword(s):  
Emergency Room ◽  
Peer Support ◽  
Statistical Tests ◽  
Bivariate Analysis ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Implementation Processes ◽  
Chi Square Test ◽  
The Government

Implementation of IMD in hospitals has decreased from the previous year and has not reached the target set by the government. Some IMD implementation processes have not been carried out according to applicable standards. So that babies do not get an IMD in accordance with existing SOPs. The purpose of this study was to determine the determinant factors associated with the implementation of the IMD by midwives in the Midwifery and Maternity Room Emergency Room (IGD) at the Anutapura General Hospital in Palu. This research method is analytical with cross sectional approach. The population of this study was that all midwives in the obstetrics emergency room and maternity room at Anutapura Palu Hospital were 37 respondents. The sample in this study is total sampling. The analysis used was univariate, and bivariate analysis using the chi square test with a confidence level of 95% (α = 0.05). The results of statistical tests on variable knowledge of midwives with the implementation of IMD p value: 0.018 (p value <0.05). APN training with the implementation of IMD p value: 0.697 (p value> 0.05). length of work with the implementation of IMD p value: 0.029 (p value <0.05). and peer support with the implementation of IMD p value: 0.007 (p value <0.05). Conclusions there is a relationship between knowledge, length of work, peer support with the implementation of the IMD, and training factors that have nothing to do with IMD implementation. The strongest factor in the relationship is peer support. It is recommended that the Anutarapura Palu Hospital be able to motivate midwives so that they can further enhance their role in the implementation and provide support to their colleagues so that the implementation of the IMD can be carried out in accordance with applicable standards.Keywords: Knowledge, APN Training, Duration of work, Implementation of IMD

Sign in / Sign up

Export Citation Format

Share Document