Dietary Intake of Flaxseed Oil since Early Stages of Life Promotes Femur Quality in Male Rats

2021 ◽  
pp. 1-6
Author(s):  
Aline D’Avila Pereira ◽  
Danielle Cavalcante Ribeiro ◽  
Letícia Monteiro da Fonseca Cardoso ◽  
Gabrielle Gracio Ribeiro ◽  
Bruna da Costa Rodrigues Quintes ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Flaxseed Oil ◽  
Male Rats ◽  
Early Stages

scholarly journals Effect of exogenous melatonin and flaxseed oil on the expression state of MT1 receptors in rat skin under light deprivation

2021 ◽  
Vol 27 (2) ◽  
pp. 30-38
Author(s):  
I.S. Sobolevskaya ◽  
M.I. Krasnobaeva ◽  
O.D. Myadelets
Keyword(s):  
Flaxseed Oil ◽  
Hair Follicles ◽  
Melatonin Receptors ◽  
Male Rats ◽  
Target Cells ◽  
Special Role ◽  
Negative Consequences ◽  
Light Deprivation ◽  
Exogenous Melatonin ◽  
Corrective Effect

Most of the skin cells have their own autonomous functional circadian system, which is able to control physiological and biochemical processes in the general integument. A special role in these processes is assigned to the “clock” hormone of the pineal gland, melatonin, which acts on target cells through specific receptors (MT1, MT2, MT3 and RORα). Any disturbance of circadian rhythms can lead to rearrangements (disturbances) in the receptor apparatus of the cells of the general cover, which require a certain correction. Consequently, there is a need to search for effective and reliable drugs that will prevent the negative consequences caused by chronodestruction. In the present work, we studied the effectiveness of the effect of exogenous melatonin and flaxseed oil on the expression of MT1 receptors in the general coat of rats under light deprivation. An experimental study was carried out on 130 white outbred male rats (170-220 g), which were randomly divided into 5 groups: intact, light deprivation animals, light deprivation animals, which were injected intragastrically with flaxseed oil and melatonin. On days 7, 14 and 21, histological material was taken (fragments of the skin of the interscapular region of the back). For immunohistochemical studies, serial sections were stained using MTNR1A polyclonal antibodies. For morphometric data analysis, the Image Scope Color and ImageJ computer programs were used. All statistical data processing was performed using the Statistica 10.0 software. Differences were considered significant at a significance level of less than 0.01 (p <0.01). In the course of the experiment, it was found that light deprivation contributes to a change in the activity of expression of the MT1 melatonin receptors in the epidermis, sebaceous glands and hair follicles. Studies have shown that the administration of flaxseed oil, melatonin, and their combination to rats with desynchronosis is accompanied by the leveling of the adverse effect of desynchronosis on the studied parameters of MT1 receptors. The most pronounced corrective effect on the expression of MT1 receptors is observed with the introduction of exogenous melatonin on the 21st day of the experiment.

Body composition in male rats subjected to early weaning and treated with diet containing flour or flaxseed oil after 21 days until 60 days

2015 ◽  
Vol 6 (6) ◽  
pp. 553-557 ◽  
Author(s):  
B. Ferolla da Camara Boueri ◽  
C. Ribeiro Pessanha ◽  
L. Rodrigues da Costa ◽  
M. R. Ferreira ◽  
H. Saldanha Melo ◽  
...  
Keyword(s):  
Body Composition ◽  
Body Mass ◽  
Control Diet ◽  
Flaxseed Oil ◽  
Male Rats ◽  
Bone Area ◽  
Early Weaning ◽  
Mineral Density ◽  
X Ray ◽  
Flaxseed Flour

The aim of this study was analyzed if the flour or flaxseed oil treatment contributes to body composition in male rats subjected to early weaning. Pups were weaned for separation from mother at 14 (early weaning, EW) and 21 days (control, C). At 21 days, part of the pups was evaluated (C21 v. EW21). After 21 days, control (C60) was fed with control diet. EW was divided in control (EWC60); flaxseed flour (EWFF60); flaxseed oil (EWFO60) diets until 60 days. Body mass, length and body composition by dual-energy X-ray absorptiometry were determined. EW21 (v. C21) and EWC60 (v. C60 and EWFF60) showed lower (P<0.05) mass, length and body composition. EWFO60 (v. C60 and EWFF60) showed lower (P<0.05) body mass and length, body and trunk lean mass, bone mineral density and content and bone area. Flaxseed flour, in comparison with flaxseed oil, contributes to recovery of body composition after early weaning.

Brain development in male rats subjected to early weaning and treated with diet containing flour or flaxseed oil after 21 days until 60 days

2015 ◽  
Vol 6 (4) ◽  
pp. 268-271 ◽  
Author(s):  
C. R. Pessanha ◽  
B. Ferolla da Camara Boueri ◽  
L. Rodrigues da Costa ◽  
M. Rocha Ferreira ◽  
H. Saldanha Melo ◽  
...  
Keyword(s):  
Brain Development ◽  
Developmental Plasticity ◽  
Prime Factor ◽  
Control Diet ◽  
Flaxseed Oil ◽  
Male Rats ◽  
Lower Body ◽  
Early Weaning ◽  
Brain Mass ◽  
Flaxseed Flour

The precocious interruption of lactation is a prime factor for developmental plasticity. Here we analyzed whether flour or flaxseed oil treatment contributes to body and brain mass in male rats subjected to early weaning. Pups were weaned for separation from their mother at 14 (early weaning, EW) and 21 days (control, C). At 21 days, some of the pups were evaluated (C21 v. EW21). After 21 days, control pups (C60) were fed a control diet. EW pups were divided into those fed a control diet (EWC60), those given flaxseed flour (EWFF60), and those given flaxseed oil (EWFO60) until 60 days. EW21 showed lower body and absolute brain mass and higher relative brain mass. At 60 days, EWC60 and EWFO60 had lower body mass. With regard to relative brain mass, EWC60 was heavier; EWFO60 had lower values compared with EWC60 and higher values compared with C60 and EWFF60. These results indicated that flaxseed flour, in comparison with flaxseed oil, contributes to brain development after EW.

scholarly journals Role of Flaxseed Oil Against Lead Toxicity in Liver of Male Rats

2020 ◽  
Vol 5 (1) ◽  
pp. 84-96
Author(s):  
Aljedaani HM ◽  
Shaikh Omar AKM ◽  
Elnaggar MHR
Keyword(s):  
Lead Toxicity ◽  
Flaxseed Oil ◽  
Male Rats

scholarly journals Early Long-Term Memory Impairment and Changes in the Expression of Synaptic Plasticity-Associated Genes, in the McGill-R-Thy1-APP Rat Model of Alzheimer's-Like Brain Amyloidosis

2021 ◽  
Vol 12 ◽  
Author(s):  
Martín Habif ◽  
Sonia Do Carmo ◽  
María Verónica Báez ◽  
Natalia Claudia Colettis ◽  
Magalí Cecilia Cercato ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Learning And Memory ◽  
Memory Deficits ◽  
Male Rats ◽  
Long Term Memory ◽  
Aβ Oligomers ◽  
Term Memory ◽  
Early Stages ◽  
Human Pathology

Accruing evidence supports the hypothesis that memory deficits in early Alzheimer Disease (AD) might be due to synaptic failure caused by accumulation of intracellular amyloid beta (Aβ) oligomers, then secreted to the extracellular media. Transgenic mouse AD models provide valuable information on AD pathology. However, the failure to translate these findings to humans calls for models that better recapitulate the human pathology. McGill-R-Thy1-APP transgenic (Tg) rat expresses the human amyloid precursor protein (APP751) with the Swedish and Indiana mutations (of familial AD), leading to an AD-like slow-progressing brain amyloid pathology. Therefore, it offers a unique opportunity to investigate learning and memory abilities at early stages of AD, when Aβ accumulation is restricted to the intracellular compartment, prior to plaque deposition. Our goal was to further investigate early deficits in memory, particularly long-term memory in McGill-R-Thy1-APP heterozygous (Tg+/–) rats. Short-term- and long-term habituation to an open field were preserved in 3-, 4-, and 6-month-old (Tg+/–). However, long-term memory of inhibitory avoidance to a foot-shock, novel object-recognition and social approaching behavior were seriously impaired in 4-month-old (Tg+/–) male rats, suggesting that they are unable to either consolidate and/or evoke such associative and discriminative memories with aversive, emotional and spatial components. The long-term memory deficits were accompanied by increased transcript levels of genes relevant to synaptic plasticity, learning and memory processing in the hippocampus, such as Grin2b, Dlg4, Camk2b, and Syn1. Our findings indicate that in addition to the previously well-documented deficits in learning and memory, McGill-R-Thy1-APP rats display particular long-term-memory deficits and deep social behavior alterations at pre-plaque early stages of the pathology. This highlights the importance of Aβ oligomers and emphasizes the validity of the model to study AD-like early processes, with potentially predictive value.

scholarly journals Darapladib inhibits atherosclerosis development in type 2 diabetes mellitus Sprague-Dawley rat model

2018 ◽  
Vol 52 (2) ◽  
pp. 69-75 ◽  
Author(s):  
Titin Andri Wihastuti ◽  
Teuku Heriansyah ◽  
Hanifa Hanifa ◽  
Sri Andarini ◽  
Zuhrotus Sholichah ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Rat Model ◽  
Foam Cells ◽  
Male Rats ◽  
Sprague Dawley ◽  
Early Stages ◽  
Sprague Dawley Rat

AbstractObjective. Increase in the low-density lipoprotein (LDL) level in diabetes mellitus and atherosclerosis is related to lipoprotein associated phospholipase A2 (Lp-PLA2). Lp-PLA2 is an enzyme that produces lysophosphatidylcholine (LysoPC) and oxidized nonesterified fatty acids (oxNEFA). LysoPC regulates inflammation mediators, including intra-cellular adhesion molecule-1 (ICAM-1). Darapladib is known as a Lp-PLA2 specific inhibitor. The aim of this study was to reveal the effect of darapladib on the foam cell number, inducible nitric oxide synthase (iNOS), and ICAM-1 expression in aorta at early stages of the atherosclerosis in type 2 diabetes mellitus Sprague-Dawley rat model.Methods. Thirty Sprague-Dawley male rats were divided into 3 main groups: control, rats with type 2 diabetes mellitus (T2DM), and T2DM rats treated with darapladib (T2DM-DP). Each group was divided into 2 subgroups according the time of treatment: 8-week and 16-week treatment group. Fasting blood glucose, insulin resistance, and lipid profile were measured and analyzed to ensure T2DM model. The foam cells number were detected using hematoxylin-eosin (HE) staining and the expression of iNOS and ICAM-1 was analyzed using double immunofluorescence staining.Results. Induction of T2DM in male Sprague-Dawley rats after high fat diet and streptozotocin injection was confirmed by elevated levels of total cholesterol and LDL and increased fasting glucose and insulin levels compared to controls after both times of treatment. Moreover, T2DM in rats induced a significant increase (p<0.05) in the foam cells number and iNOS and ICAM-1 expression in aorta compared to controls after both treatment times. Darapladib treatment significantly reduced (p<0.05) foam cells number as well as iNOS expression in aorta in rats with T2DM after both treatment times. A significant decrease (p<0.05) in ICAM-1 expression in aorta was observed after darapladib treatment in rats with T2DM only after 8 weeks of treatment.Conclusion. Our data indicate that darapladib can decrease the foam cells number, iNOS, and ICAM-1 expression in aorta at the early stages of atherosclerosis in T2DM rat model.

scholarly journals PROFILE OF SEX STEROIDS AT EARLY STAGES OF LIVER METASTASIS FROM SARCOMA 45 IN MALE RATS

2017 ◽  
Vol 4 (1) ◽  
pp. 55-62
Author(s):  
I. V. Kaplieva ◽  
E. M. Frantsiyants ◽  
L. K. Trepitaki ◽  
N. D. Cheryarina
Keyword(s):  
Liver Metastasis ◽  
Sex Steroids ◽  
Male Rats ◽  
Early Stages ◽  
Sarcoma 45

Anti-inflammatory therapy in acute periods of chronic ischemic retinal injury in rats

2017 ◽  
pp. 51-57
Author(s):  
Е.М. Клочихина ◽  
С.А. Гаврилова
Keyword(s):  
Arachidonic Acid ◽  
Vascular Occlusion ◽  
Ischemic Injury ◽  
Retinal Ischemia ◽  
Male Rats ◽  
Arachidonic Acid Cascade ◽  
Ischemic Disease ◽  
Early Stages ◽  
Retinal Injury ◽  
Retinal Vascular Occlusion

Ишемия сетчатки возникает вследствие окклюзии сосудов сетчатки или как осложнение при таких заболеваниях, как диабет, глаукома, и при других патологиях. Ишемическое повреждение связано с развитием воспалительного ответа и активацией каскада арахидоновой кислоты. Цель настоящей работы состояла в изучении влияния блокады каскада арахидоновой кислоты на развитие ишемического повреждения сетчатки глаза крысы. Методы. Ишемию сетчатки моделировали путем двусторонней окклюзии внутренних сонных артерий (ВСА) у крыс-самцов линии Вистар. Через 15 минут после окклюзии ВСА вводили интравитреально (по 2 мкл) лорноксикам (неселективный блокатор циклооксигеназ), триамцинолон (блокатор фосфолипазы А2) и физиологический раствор. Кроме того, через 24 и 48 ч после окклюзии ВСА лорноксикам вводили внутрибрюшинно (230 мкг/кг) и внутримышечно триамцинолон (571 мкг/кг). Офтальмоскопию для оценки состояния глазного дна проводили до операции, через 7, 14, 28, 56 и 180 суток после окклюзии артерий. В аналогичные сроки осуществляли энуклеацию глаз для гистологического исследования. Результаты исследования показали, что лорноксикам обладает пролонгированным протекторным эффектом на ишемизированную сетчатку, в частности, замедляет истончение сетчатки и ограничивает увеличение площади неперфузируемых капиллярных зон. В то же время эффекты триамцинолона были неоднозначными, кратковременными и развивались на фоне ухудшения общего самочувствия животных. Заключение. Полученные данные свидетельствуют о возможности применения блокаторов синтеза простагландинов для терапии ишемических заболеваний сетчатки глаза. Background. Retinal ischemia results from retinal vascular occlusion or develops as a complication of diabetes mellitus, glaucoma, and various neovascular diseases. Ischemic injury is definitely related with an inflammatory response and activation of the arachidonic acid cascade. For this reason, the aim of this study was to examine the course of changes in ischemic injury of rat retina and the effect of blocking the arachidonic acid cascade at early stages. Methods. Retinal ischemia was simulated by bilateral occlusion of internal carotid arteries (ICA) in Wistar male rats. Lornoxicam (non-selective cyclooxygenase inhibitor), triamcinolone (phospholipase A2 inhibitor), and saline were injected intravitreally at 15 min following ICA bilateral occlusion and intraperitoneally at 24 h and 48 h. The fundus was examined using ophthalmoscopy before the operation and at 7, 14, 28, 56, and 180 days following the occlusion. Enucleation was conducted for histological analysis on the same days. Results. Lornoxicam administered at early stages exerted a long-term protective effect on the ischemic retina. Effects of triamcinolone were controversial, brief, and associated with impaired general state of animals. Therefore, prostaglandin synthesis inhibitors might be used as a therapy for retinal ischemic disease.

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