RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN WILD AND CULTIVATED TETRAPLOID WHEAT

1999 ◽  
Vol 47 (4) ◽  
pp. 213-224 ◽  
Author(s):  
Li Huang ◽  
Eitan Millet ◽  
Junkang Rong ◽  
Jonathan F. Wendel ◽  
Yehoshua Anikster ◽  
...  

RFLP diversity in the nuclear genome was estimated within and among Israeli populations of wild emmer wheat (Triticum turgidumvar.dicoccoides) from a long-term study site at Ammiad (NE Israel), and from several other geographical locations. Using 55 enzyme-probe combinations, high levels of genetic diversity were revealed in wild emmer in general and within the Ammiad site. In spite of high diversity, observed heterozygosity was low and populations consisted of a patchwork of alternate multilocus homozygotes, consistent with the reproductive biology of a predominant self-fertilizing species. Retention of genetic diversity in wild emmer may be promoted by large population sizes, microhabitat diversity, and occasional gene flow through both pollen and seed. Population genetic structure in wild emmer appears to have been influenced by historical founder events as well as selective factors. Multivariate analyses indicated that individuals tend to cluster together according to their population of origin, and that there is little geographical differentiation among populations. Sampling of 12 domesticated land-races and both primitive and modern cultivars ofT. turgidumrevealed high levels of diversity and a large number of alleles that were not detected in the wild emmer populations. This may reflect a long-term domestication process in which wild, semi-domesticated, and domesticated types grew sympatrically, continuing introgression from wild populations, and perhaps also gene flow from trans-specific sources.

2015 ◽  
Vol 63 (4) ◽  
pp. 279 ◽  
Author(s):  
Josef Krawiec ◽  
Siegfried L. Krauss ◽  
Robert A. Davis ◽  
Peter B. S. Spencer

Populations in fragmented urban remnants may be at risk of genetic erosion as a result of reduced gene flow and elevated levels of inbreeding. This may have serious genetic implications for the long-term viability of remnant populations, in addition to the more immediate pressures caused by urbanisation. The population genetic structure of the generalist skink Ctenotus fallens was examined using nine microsatellite markers within and among natural vegetation remnants within a highly fragmented urban matrix in the Perth metropolitan area in Western Australia. These data were compared with samples from a large unfragmented site on the edge of the urban area. Overall, estimates of genetic diversity and inbreeding within all populations were similar and low. Weak genetic differentiation, and a significant association between geographic and genetic distance, suggests historically strong genetic connectivity that decreases with geographic distance. Due to recent fragmentation, and genetic inertia associated with low genetic diversity and large population sizes, it is not possible from these data to infer current genetic connectivity levels. However, the historically high levels of gene flow that our data suggest indicate that a reduction in contemporary connectivity due to fragmentation in C. fallens is likely to result in negative genetic consequences in the longer term.


Author(s):  
Richard Frankham ◽  
Jonathan D. Ballou ◽  
Katherine Ralls ◽  
Mark D. B. Eldridge ◽  
Michele R. Dudash ◽  
...  

Most species now have fragmented distributions, often with adverse genetic consequences. The genetic impacts of population fragmentation depend critically upon gene flow among fragments and their effective sizes. Fragmentation with cessation of gene flow is highly harmful in the long term, leading to greater inbreeding, increased loss of genetic diversity, decreased likelihood of evolutionary adaptation and elevated extinction risk, when compared to a single population of the same total size. The consequences of fragmentation with limited gene flow typically lie between those for a large population with random mating and isolated population fragments with no gene flow.


2011 ◽  
Vol 279 (1733) ◽  
pp. 1560-1566 ◽  
Author(s):  
Lee Koren ◽  
Shinichi Nakagawa ◽  
Terry Burke ◽  
Kiran K. Soma ◽  
Katherine E. Wynne-Edwards ◽  
...  

Potential mechanistic mediators of Darwinian fitness, such as stress hormones or sex hormones, have been the focus of many studies. An inverse relationship between fitness and stress or sex hormone concentrations has been widely assumed, although empirical evidence is scarce. Feathers gradually accumulate hormones during their growth and provide a novel way to measure hormone concentrations integrated over time. Using liquid chromatography–tandem mass spectrometry, we measured testosterone, corticosterone and cortisol in the feathers of house sparrows ( Passer domesticus ) in a wild population which is the subject of a long-term study. Although corticosterone is considered the dominant avian glucocorticoid, we unambiguously identified cortisol in feathers. In addition, we found that feathers grown during the post-nuptial moult in autumn contained testosterone, corticosterone and cortisol levels that were significantly higher in birds that subsequently died over the following winter than in birds that survived. Thus, feather steroids are candidate prospective biomarkers to predict the future survival of individuals in the wild.


2015 ◽  
Vol 71 ◽  
pp. 69-79 ◽  
Author(s):  
Martijn Hammers ◽  
Sjouke A. Kingma ◽  
Kat Bebbington ◽  
Janske van de Crommenacker ◽  
Lewis G. Spurgin ◽  
...  

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Perla Farhat ◽  
Sonja Siljak-Yakovlev ◽  
Nicolas Valentin ◽  
Carlos Fabregat ◽  
Silvia Lopez-Udias ◽  
...  

Abstract Background Gene flow and polyploidy have been found to be important in Juniperus evolution. However, little evidence has been published elucidating the association of both phenomena in juniper taxa in the wild. Two main areas were studied in Spain (Eastern Iberian Range and Sierra de Baza) with both diploid and tetraploid taxa present in sympatry. Gene flow and ploidy level were assessed for these taxa and the resulted offspring. Results Twenty-two allo-triploid hybrids between J. sabina var. sabina and J. thurifera were found in the Eastern Iberian Range population. However, in the Sierra de Baza population no triploids were found. Instead, 18 allo-tetraploid hybrids between two tetraploid taxa: J. sabina var. balkanensis and J. thurifera were discovered. High genetic diversity was exhibited among the tetraploid hybrids at Sierra de Baza, in contrast to the genetically identical triploid hybrids at the Eastern Iberian Range; this suggests meiotic difficulties within the triploid hybrids. In addition, unidirectional gene flow was observed in both studied areas. Conclusion Polyploidy and hybridization can be complementary partners in the evolution of Juniperus taxa in sympatric occurrences. Juniperus was shown to be an ideal coniferous model to study these two phenomena, independently or in concert.


Parasitology ◽  
2015 ◽  
Vol 142 (8) ◽  
pp. 1033-1043 ◽  
Author(s):  
M. ZYLBERBERG ◽  
E. P. DERRYBERRY ◽  
C. W. BREUNER ◽  
E. A. MACDOUGALL-SHACKLETON ◽  
J. M. CORNELIUS ◽  
...  

SUMMARYThe impact of haematozoan infection on host fitness has received substantial attention since Hamilton and Zuk posited that parasites are important drivers of sexual selection. However, short-term studies testing the assumption that these parasites consistently reduce host fitness in the wild have produced contradictory results. To address this complex issue, we conducted a long-term study examining the relationship between naturally occurring infection withHaemoproteusandPlasmodium, and lifetime reproductive success and survival of Mountain White-crowned Sparrows. Specifically, we tested the hypothesis that birds infected with haematozoan parasites have reduced survival (as determined by overwinter return rates) and reproductive success. Contrary to expectation, there was no relationship betweenHaemoproteusandPlasmodiuminfection and reproduction or survival in males, nor was there a relationship betweenPlasmodiuminfection and reproduction in females. Interestingly,Haemoproteus-infected females had significantly higher overwinter return rates and these females fledged more than twice as many chicks during their lifetimes as did uninfected females. We discuss the impact of parasitic infections on host fitness in light of these findings and suggest that, in the case of less virulent pathogens, investment in excessive immune defence may decrease lifetime reproduction.


1998 ◽  
Vol 120 (1) ◽  
pp. 81-86 ◽  
Author(s):  
LIAN-CHEN WANG

Parasitic infections have been reported to be relatively common among the Southeast Asian labourers in Taiwan. This study, conducted in 1992–6, was designed to determine the temporal changes of the prevalence. Faecal specimens were examined by the formalin-ethyl acetate sedimentation technique and blood samples screened using the quantitative buffy coat technique and confirmed by Giemsa stained blood smear. The overall prevalence of intestinal parasitic infections was 10·3%. The annual prevalence decreased from 33·3% in 1992–3 to 4·6% in 1995–6. The Thai (12·0%) and Indonesian (11·1%) had a higher prevalence than the Malaysian (6·7%) and Filipinos (5·9%). Opisthorchis viverrini was the most important parasite in the Thai and Trichuris trichiura in the remaining groups. Moreover, no blood parasites were found in the labourers. The dramatic temporal decline in the intestinal parasitic infections suggests that limiting the entry of infected persons, periodic follow-ups, and immediate treatment of sporadic cases are necessary in preventing transmission of non-indigenous parasites through large population change.


Agronomy ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 107 ◽  
Author(s):  
Anssi Vuorinen ◽  
Ruslan Kalendar ◽  
Tzion Fahima ◽  
Helena Korpelainen ◽  
Eviatar Nevo ◽  
...  

2008 ◽  
Vol 105 (40) ◽  
pp. 15464-15469 ◽  
Author(s):  
Nikos Poulakakis ◽  
Scott Glaberman ◽  
Michael Russello ◽  
Luciano B. Beheregaray ◽  
Claudio Ciofi ◽  
...  

Giant tortoises, a prominent symbol of the Galápagos archipelago, illustrate the influence of geological history and natural selection on the diversification of organisms. Because of heavy human exploitation, 4 of the 15 known species (Geochelone spp.) have disappeared. Charles Darwin himself detailed the intense harvesting of one species, G. elephantopus, which once was endemic to the island of Floreana. This species was believed to have been exterminated within 15 years of Darwin's historic visit to the Galápagos in 1835. The application of modern DNA techniques to museum specimens combined with long-term study of a system creates new opportunities for identifying the living remnants of extinct taxa in the wild. Here, we use mitochondrial DNA and microsatellite data obtained from museum specimens to show that the population on Floreana was evolutionarily distinct from all other Galápagos tortoise populations. It was demonstrated that some living individuals on the nearby island of Isabela are genetically distinct from the rest of the island's inhabitants. Surprisingly, we found that these “non-native” tortoises from Isabela are of recent Floreana ancestry and closely match the genetic data provided by the museum specimens. Thus, we show that the genetic line of G. elephantopus has not been completely extinguished and still exists in an intermixed population on Isabela. With enough individuals to commence a serious captive breeding program, this finding may help reestablish a species that was thought to have gone extinct more than a century ago and illustrates the power of long-term genetic analysis and the critical role of museum specimens in conservation biology.


Energies ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5786
Author(s):  
Filipe Quintal ◽  
Daniel Garigali ◽  
Dino Vasconcelos ◽  
Jonathan Cavaleiro ◽  
Wilson Santos ◽  
...  

This paper presents the development and evaluation of EnnerSpectrum, a platform for electricity monitoring. The development was motivated by a gap between academic, fully custom-made monitoring solutions and commercial proprietary monitoring approaches. EnnerSpectrum is composed of two main entities, the back end, and the Gateway. The back end is a server comprised of flexible entities that can be configured to different monitoring scenarios. The Gateway interacts with equipment at a site that cannot interact directly with the back end. The paper presents the architecture and configuration of EnnerSpectrum for a long-term case study with 13 prosumers of electricity for approximately 36 months. During this period, the proposed system was able to adapt to several building and monitoring conditions while acquiring 95% of all the available consumption data. To finalize, the paper presents a set of lessons learned from running such a long-term study in the real world.


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