Chemical Composition, Antibacterial Activity using Micro-broth Dilution Method and Antioxidant Activity of Essential Oil and Water Extract from Aerial Part of Tunisian Thymus algeriensis Boiss. & Reut.

2022 ◽  
pp. 1-16
Author(s):  
Amal Ghorbel ◽  
Jawhar Fakhfakh ◽  
Vincent Brieudes ◽  
Maria Halabalaki ◽  
Stéphane Fontanay ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Aerial Part ◽  
Water Extract ◽  
Dilution Method ◽  
Thymus Algeriensis ◽  
Broth Dilution

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

scholarly journals Chemical Composition and Antibacterial and Antioxidant Activity of a Citrus Essential Oil and Its Fractions

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2888
Author(s):  
Carmen M. S. Ambrosio ◽  
Gloria L. Diaz-Arenas ◽  
Leidy P. A. Agudelo ◽  
Elena Stashenko ◽  
Carmen J. Contreras-Castillo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Biological Activities ◽  
E Coli ◽  
Beneficial Bacterium ◽  
Minor Compounds ◽  
Antibacterial And Antioxidant Activity ◽  
High Relative Amount

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.

Chemical composition, antioxidant and antimicrobial activities of essential oil of Thymus algeriensis wild-growing in Libya

2013 ◽  
Vol 8 (5) ◽  
pp. 504-511 ◽  
Author(s):  
Abdulhmid Giweli ◽  
Ana Džamić ◽  
Marina Soković ◽  
Mihailo Ristić ◽  
Petar Marin
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Activities ◽  
Practical Application ◽  
Dpph Assay ◽  
High Antimicrobial Activity ◽  
Thymus Algeriensis ◽  
Antioxidant And Antimicrobial Activities

AbstractThe composition of essential oil isolated from Thymus algeriensis growing wild in Libya was analyzed by GC and GC-MS. The essential oil was characterized with thymol (38.50%) as the major component. The oil was screened for antioxidant activity using DPPH assay, and compared to thymol and carvacrol. Antioxidant activity was high, with the IC50 of 0.299 mg/ml, compared to 0.403 and 0.105 mg/ml for thymol and carvacrol, and 0.0717 mg/ml for BHA. In addition, antimicrobial activity was tested against eight bacteria and eight fungi. T. algeriensis oil showed inhibitory activity against tested bacteria at 0.001–0.05 mg/ml, while bactericidal activity (MBC) was achieved at 0.0025–0.05 mg/ml. For antifungal activity MICs ranged 0.0005–0.025 mg/ml and MFC 0.001–0.05 mg/ml. High antimicrobial activity against the fungi in particular suggests that the essential oil of Thymus algeriensis could have a useful practical application.

Chemical Composition, Antioxidant, Antibacterial, Cytotoxicity, and Hemolyses Activity of Essential Oils from Flower of Matricaria chamomilla var. Chamomilla

2020 ◽  
Vol 18 (3) ◽  
pp. 224-232
Author(s):  
Behnam Mahdavi ◽  
Fereshteh Ghorat ◽  
Mahda S. Nasrollahzadeh ◽  
Mahmood Hosseyni-Tabar ◽  
Hassan Rezaei-Seresht
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Human Cancer ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Matricaria Chamomilla ◽  
Human Cancer Cells ◽  
Concentration Method

Introduction: In this research, we have reported the chemical composition and bioactivity of the essential oil from flowers of Matricaria chamomilla var. Chamomilla (MCCO) for the first time. The essential oil was extracted using a Clevenger-type apparatus. The chemical composition of the essential oil was identified by chromatography methods. DPPH Radical Scavenging Activity (RSA), β-carotene bleaching (BCB), and Ferrous Ion Chelating ability (FIC) were chosen to evaluate the MCCO antioxidant activity. Disc diffusion assay and Mínimum Inhibitory Concentration method (MIC) were selected to investigate antibacterial activity of MCCO. Hemolytic activity of MCCO on Red Blood Cells (RBCs) was measured through optical density. Methods: MTT method was used to determine the cytotoxicity effects of MCCO on human cáncer cells. MCCO was dominated byoxygenated sesquiterpenes (59.01%). α-Bisabolone oxide A (35.74%), α- bisabolol oxide A (19.07%), (Z)-β-farnesene (6.63%), and chamazulene (6.46%)wasfound as themajorcomponents oftheessentialoil. The oil represented an acceptable antioxidant activity. For antibacterial activity, MCCO prevented the growth of all selected microorganisms. The oil can be considered as a strong antibacterial agent as well as anantioxidant. Results: MCCO showed a low hemolytic rate (below 1.5%) on RBCs. The safety of MCCO as a food additive or other uses was suggested by the hemolysis result. Conclusions: MCCO was identified as a weak agent in the cytotoxicity assay.

scholarly journals Chemical Composition and Biological Activity of Salvia Verbenaca Essential Oil

2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Marisa Canzoneri ◽  
Maurizio Bruno ◽  
Sergio Rosselli ◽  
Alessandra Russo ◽  
Venera Cardile ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Octadecenoic Acid ◽  
Perennial Herb ◽  
Dilution Method ◽  
In Vitro Activity ◽  
Hexadecanoic Acid ◽  
Aerial Parts ◽  
Broth Dilution

Salvia verbenaca L. (syn. S. minore) is a perennial herb known in the traditional medicine of Sicily as “spaccapetri” and is used to resolve cases of kidney stones, chewing the fresh leaves or in decoction. The chemical composition of the essential oil obtained from aerial parts of S. verbenaca collected in Piano Battaglia (Sicily) on July 2009, was analyzed by GC and GC-MS. The oil was strongly characterized by fatty acids (39.5%) and carbonylic compounds (21.2%), with hexadecanoic acid (23.1%), ( Z)-9-octadecenoic acid (11.1%) and benzaldehyde (7.3%) as the main constituents. The in vitro activity of the essential oil against some microorganisms in comparison with chloramphenicol by the broth dilution method was determined. The oil exhibited a good activity as inhibitor of growth of Gram + bacteria.

scholarly journals Chemical Characterization and In vitro Antimicrobial Activity of Caralluma europaea Essential Oil and Its Synergistic Potential with Conventional Antibiotics

2019 ◽  
pp. 1-11 ◽  
Author(s):  
Loubna Ait Dra ◽  
Abdellah Aghraz ◽  
Brahim Boualy ◽  
Saadia Oubaassine ◽  
Mustapha Barakate ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Aerial Part ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Acid Oxidation ◽  
Pharmaceutical Industries

Aim: This study was carried out to investigate the antioxidant and antimicrobial activities of essential oil from the aerial part of Caralluma europaea and to evaluate the synergistic potential between essential oil and antibiotics. Methodology: The chemical composition, antioxidant, antimicrobial activities and synergetic interaction between antimicrobial agents and essential oil isolated by hydrodistillation from the aerial part of C. europaea were evaluated. The chemical composition was analyzed by a Gas chromatography/mass spectrometry (GC/MS) system. Antioxidant activity was measured employing three methods: scavenging of free radical DPPH, reducing power assay and the inhibition of linoleic acid oxidation. The antimicrobial activity of essential oil against microbial strains was qualitatively and quantitatively assessed by the presence or absence of inhibition zones diameters, and MIC values. The in vitro association between essential oil and some commercial antibiotics was also investigated. Results: The GC/MS analysis shows that a total of 21 constituents were identified and the main compounds were Terpinolene (19.5%), α-Terpinene (16.2%) and Linalool (15.3%). Antioxidant study showed that essential oil exhibited antioxidant activity with IC50 values ranging from 0.32 mg/ml to 1.45 mg/ml. The results of antimicrobial activity showed that the essential oil had an inhibitory effect against the majority of tested microorganisms except K. pneumonia and  P. aeruginosa. Gram-positive bacteria were found to be more sensitive than Gram-negative ones. Furthermore, essential oil approved an interesting antifungal activity against yeast species. Out of 25 combinations tested 64% showed total synergism, 20% had a partial synergistic interaction and 16% showed no effect. The best synergistic effect was obtained with the combination essential oil-gentamycin. Conclusion: Our results are of a great importance and suggest that C. europaea essential oil contain bioactive compounds with antioxidant and antimicrobial properties with possible applications in the food and pharmaceutical industries.

scholarly journals Antioxidant and antibacterial activities of Ischaemum indicum leaves extracted using different solvents

2019 ◽  
Vol 15 (3) ◽  
pp. 436-440
Author(s):  
Fithrotul Auwaliyah ◽  
Hamdan Dwi Rizqi ◽  
Adi Setyo Purnomo ◽  
Sri Fatmawati
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Potential Candidate ◽  
Dpph Assay ◽  
Abts Assay ◽  
Water Extracts ◽  
Broth Dilution

The aim of this study was to evaluate antioxidant and antibacterial activities of Ischaemum indicum leaves extracts based on different polarity solvents: water, methanol, ethyl acetate, dichloromethane, and n-hexane. The antioxidant activity was evaluated using ABTS and DPPH methods, while the antibacterial activity was determined by broth dilution method against Pseudomonas aeruginosa and Bacillus subtilis. The results showed that water and methanol extracts of I. indicum gave the highest antioxidant activity in ABTS assay with an IC50 73.80 and 46.32 µg/mL, respectively. On the other hand, The IC50 on DPPH assay for  water extracts was 117.51 µg/mL, this value was higher than that of methanol extract with no inhibition. Methanol and water extracts showed the antibacterial activity against B. subtilis with inhibition of 48.62 and 45.11%, whereas inhibition of P. aeruginosa were 31.95 and 30.31%, respectively. This study denoted that I. indicum is a new potential candidate as antioxidant source.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil and Extracts of Citharexylum spinosum Flowers from Thailand

2014 ◽  
Vol 9 (5) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Ae Mar ◽  
Patcharee Pripdeevech
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
The Other ◽  
Total Phenol Content ◽  
Chemical Compositions ◽  
Phenol Content ◽  
Bacterial Strains

The chemical composition and antibacterial and antioxidant activities of the essential oil and various solvent extracts of Citharexylum spinosum flowers are reported. The chemical compositions were determined by GC-MS with 151 volatile constituents identified. Methyl benzoate, piperitone, maltol, and maple furanone were the major constituents. All extracts were tested for their antibacterial activity against eight microorganisms. The flower oil had the greatest antibacterial activity against all bacterial strains (MIC values of 31.2 μg/mL), while the other solvent extracts had MIC values ranging from 31.2 to 1000 μg/mL. The essential oil had the highest antioxidant activity and total phenol content with IC50 values of 62.7 and 107.3 μg/mL, respectively.

Sign in / Sign up

Export Citation Format

Share Document