Reduction of hepatorenal and pancreatic damage by Ferula elaeochytris extract in STZ induced diabetic rats

AbstractBackgroundDiplotaenia turcica has been used traditionally to diabetes treatment. In this study, the effects of D. turcica root extract (DT) on diabetes mellitus induced by streptozotocin (STZ) were investigated.Materials and methodsIn this study, 78 male rats were used, rats were divided into 9 groups randomly. In diabetic groups, STZ was given a single dose of 45 mg/kg by intraperitoneally. DT (50, 100 and 200 mg/kg) and glibenclamide (5 mg/kg) were given by orally. Blood and pancreas tissue samples were taken for biochemical and pathological tests.ResultsIt was found that glucose levels decreased, and insulin levels increased in the treatment groups compared with the diabetes group. In addition, only in 200 mg/kg DT dose group was found to decrease HbA1c levels. Pancreatic tissue analysis showed that MDA levels decreased and GSH levels and CAT, SOD, GSH-Px and GSH-R activities increased in diabetic rats treated with DT. Histopathological and immunohistochemical examinations of the pancreas showed significant improvements in the treatment with DT.ConclusionThese results clearly show the antioxidant property of DT. The findings of this study showed that increased doses of DT may have a therapeutic effect on STZ-induced pancreatic damage.

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Leaf Extracts of Anchomanes difformis Ameliorated Kidney and Pancreatic Damage in Type 2 Diabetes

Plants ◽

10.3390/plants10020300 ◽

2021 ◽

Vol 10 (2) ◽

pp. 300

Author(s):

Toyin Dorcas Alabi ◽

Nicole L. Brooks ◽

Oluwafemi O Oguntibeju

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Microvascular Complications ◽

Diabetic Rats ◽

Leaf Extracts ◽

Pancreatic Damage ◽

Body Weights ◽

Male Wistar Rats ◽

End Stage

Kidney disease in diabetes is one of the common microvascular complications of diabetes mellitus implicated in end-stage renal failure. This study explored the ability of Anchomanes difformis to ameliorate kidney and pancreatic damage in type 2 diabetes mellitus using male Wistar rats. Two weeks of fructose (10%) administration followed by streptozotocin (40 mg/kg) were used to induce type 2 diabetes. Leaf extract (aqueous) of Anchomanes difformis (200 mg and 400 mg/kgBW) was administered orally for six weeks. Body weights were monitored, urea and creatinine were measured. Interleukins (IL)-1β, IL-6, IL-10, IL-18, and TNFα were measured in the kidney lysate. CAT, SOD, ORAC, FRAP, and MDA levels were also evaluated in the kidney. Transcription factors (Nrf2 and NF-ĸB/p65) and apoptotic markers (Bcl2 and caspase 3) were investigated in the kidney. Histological sections of the pancreas and kidney tissues were examined for any visible pathology. Supplementation with Anchomanesdifformis enhanced antioxidant status, modulated inflammatory response, and reduced apoptosis in the kidney. It also restored the kidney and pancreatic histoarchitecture of the treated diabetic rats. The pathophysiology associated with diabetic nephropathy and pancreatic damage showcase the importance of exploring the use of antidiabetic, nephroprotective agents such as Anchomanes difformis to kidney damage in type 2 diabetes.

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Effect of Katakakhadiradi Kashayam on Lipid Profile and Pancreatic Damage in Type II Diabetes Mellitus

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i28b31547 ◽

2021 ◽

pp. 135-141

Author(s):

Angelie Jessica Subbiah ◽

M. Kavimani ◽

Mukilan Ramadoss ◽

Mudiganti Ram Krishna Rao ◽

K. Prabhu

Keyword(s):

Lipid Profile ◽

Tissue Damage ◽

Serum Lipid ◽

Total Cholesterol Level ◽

Hdl Cholesterol ◽

Pancreatic Tissue ◽

Diabetic Rats ◽

Serum Lipid Profile ◽

Standard Drug ◽

Pancreatic Damage

The lipid pattern and levels in diabetic patient are the same as those for subjects with cardiovascular disease. The tactic underlying the oral hypoglycemic agent is to adjust the lipid profile; which can be achieved by herbal therapy. The use of herbs and formulations for attenuation of hyperglycemia and to aid protection against the pancreatic damage is clinically very important. This study was intended to find the efficacy of Katakakhadiradi Kashayam(KKK)on lipid profile and pancreatic tissue damage in streptozotocin-nicotinamide (SN) induced diabetic rats. The diabetic rats were treated with Katakakhadiradi Kashyam orally at doses of 100, 200 and 300 mg/kg/bw. For 28 days and compared with the standard drug Glibenclamide. After the kashayam treatment triglyceride, HDL-cholesterol and total cholesterol level were assayed and pancreatic tissue damage caused by streptozotocin was analysed by histology study.Katakakhadiradi kashayam could restore the serum lipid profile by controlling the blood glucose level and reduce the pancreatic injury in diabetic rats. Supplementation of Katakakhadiradi kashayam showed a significant improvement in the serum lipid profile thus helping in retarding the secondary complications.

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Combination of Obestatin and Bone Marrow Mesenchymal Stem Cells Prevents Aggravation of Endocrine Pancreatic Damage in Type II Diabetic Rats

International Journal of Stem Cells ◽

10.15283/ijsc17035 ◽

2017 ◽

Vol 10 (2) ◽

pp. 129-143 ◽

Cited By ~ 4

Author(s):

Noha I Hussien ◽

Nesrine Ebrahim ◽

Ola M Mohammed ◽

Dina Sabry

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Diabetic Rats ◽

Type Ii ◽

Pancreatic Damage ◽

Marrow Mesenchymal Stem Cells

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Evaluation of anti-hyperglycemic and anti-hyperlipidemic effects of Naravelia zeylanica in streptozotocin- induced diabetic rats

International Journal of Phytomedicine ◽

10.5138/09750185.1825 ◽

2017 ◽

Vol 8 (4) ◽

pp. 482

Author(s):

Rajakalanithi A ◽

Swasthika P ◽

Sujatha S

Keyword(s):

Blood Glucose ◽

Skin Diseases ◽

Serum Insulin ◽

Diabetic Rats ◽

Metabolic Enzyme ◽

Standard Drug ◽

Pancreatic Damage ◽

Diabetic Model ◽

Treatment Of Diabetes ◽

Carbohydrate Enzymes

Naravelia zeylanica DC (Ranunculaceae), a woody climber, have been used from ancient times to treat various ailments like rheumatoid arthritis, skin diseases, wound and ulcer. The present study was designed to investigate the anti-hyperglycemic activity of methanolic extract of Naravelia zeylanica (NZYM) using experimental diabetic model. Diabetes was induced in wistar rats by a single dose of streptozotocin (55 mg/kg i.p.) (STZ) and treated with NZYM at doses of 100 and 200 mg/kg for 45 days. Glibenclamide (5 mg/kg b.w.) was used as standard drug. Blood glucose and body weight were monitored at regular intervals and the levels of serum insulin, lipid and the carbohydrate metabolic enzyme in the liver were measured at the end of the study. Oral administration of NZYM and glibenclamide significantly reduced the blood glucose level (p<0.05), with increased serum insulin and significant alteration in lipid profiles and liver carbohydrate enzymes (p<0.05) after 45 days. Furthermore, the biochemical parameters correlated with the histopathological changes in the pancreas of STZ-induced diabetic rats, which structurally proved the efficacy of NZYM. The findings suggest that NZYM possess anti-hyperglycemic activity and anti-hyperlipidemic properties and restored STZ-induced pancreatic damage in diabetic rats. NZYM might therefore have a beneficial effect in treatment of diabetes mediated through regulation of carbohydrate metabolic enzyme activities.

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Sites of Insulin Action Using Ferritin Labeling

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066759 ◽

1971 ◽

Vol 29 ◽

pp. 540-541

Author(s):

Burton B. Silver ◽

Ronald S. Nelson

Keyword(s):

Electron Microscopy ◽

Binding Sites ◽

Anterior Pituitary ◽

Insulin Action ◽

Diabetic Rats ◽

Insulin Antibody ◽

Fat Pad ◽

Target Organs ◽

Uranium Salts ◽

Sugar Levels

Some investigators feel that insulin does not enter cells but exerts its influence in some manner on the cell surface. Ferritin labeling of insulin and insulin antibody was used to determine if binding sites of insulin to specific target organs could be seen with electron microscopy.Alloxanized rats were considered diabetic if blood sugar levels were in excess of 300 mg %. Test reagents included ferritin, ferritin labeled insulin, and ferritin labeled insulin antibody. Target organs examined were were diaphragm, kidney, gastrocnemius, fat pad, liver and anterior pituitary. Reagents were administered through the left common carotid. Survival time was at least one hour in test animals. Tissue incubation studies were also done in normal as well as diabetic rats. Specimens were fixed in gluteraldehyde and osmium followed by staining with lead and uranium salts. Some tissues were not stained.

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