Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract

Aim. Cytokines and chemokines may be involved in the onset of oral ulcer in Behcet’s disease. The aim of our study is to assess the cytotoxic effects of proinflammatory cytokines and chemokines on reconstructed oral mucosal cell line (TR146) when treated with different concentrations of neutrophil elastase (NE). Objective. For this purpose, a culture of the oral mucosal model (OMM) prepared from a cell line derived from an oral squamous cell carcinoma of buccal mucosa (TR146) is treated with different concentrations of neutrophil elastase. The cultures were incubated for 4- and 24-hour intervals and designed as follows: culture + artificial saliva served as the negative control; culture + 0.01% SLS (sodium lauryl sulphate) served as the positive control; and culture + NE (10, 50, 100, and 200 nM) served as the treated group. Materials and Methods. We used sandwich ELISA technique to isolate IL-1β (interleukin 1β), IL-8, and TNF-α (tumor necrosis factor). Results. We found no significant level of IL-8 and TNF-α when treated with different concentrations of neutrophil elastase after 4- and 24-hour incubation. The IL-1β level was slightly higher when treated with 100 and 200 nM NE after 24 hours of incubation although a significantly high level was observed at 100 nM NE after 4 hours of incubation. Hence, we found an increase in the level of IL-1β when stimulating the reconstructed oral mucosal model (OMM) with different concentrations of NE. This is a preliminary in vitro study; however, further research is required to evaluate the cytotoxic effects of cytokines and chemokines released when treated with NE. Moreover, high concentrations of NE are recommended to stimulate the release of cytokines and chemokines against the OMM.

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Phytochemical Analysis and Wound-Healing Activity of Noni (Morinda Citrifolia) Leaf Extract

Journal of Herbs Spices & Medicinal Plants ◽

10.1080/10496475.2020.1748159 ◽

2020 ◽

Vol 26 (4) ◽

pp. 379-393

Author(s):

Hai Trieu Ly ◽

Minh Trang Pham Nguyen ◽

Thi Kim Oanh Nguyen ◽

Thi Phuong Quynh Bui ◽

Xu Ke ◽

...

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Morinda Citrifolia ◽

Phytochemical Analysis ◽

Wound Healing Activity

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Uji aktivitas gel ekstrak daun pohpohan (Pilea trinervia W.) terhadap penyembuhan luka bakar pada kelinci (Oryctolagus cuniculus)

Kartika Jurnal Ilmiah Farmasi ◽

10.26874/kjif.v5i2.120 ◽

2017 ◽

Vol 5 (2) ◽

pp. 75

Author(s):

Via Fitria ◽

Rafiki Fahrul Arifin ◽

Nia Kurniasih

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Positive Control ◽

Test Results ◽

Activity Test ◽

Pressure Test ◽

Spreading Test ◽

Wound Healing Activity ◽

Non Parametric

Abstrak Masyarakat Indonesia mengenal dan memanfaatkan tanaman berkhasiat obat sebagai salah satu upaya dalam penanggulangan masalah kesehatan. Salah satu tanaman berkhasiat untuk menyembuhkan luka bakar adalah daun pohpohan (Pilea trinervia W.). Penelitian ini bertujuan menguji aktivitas gel ekstrak daun pohpohan (Pilea trinervia W.) terhadap penyembuhan luka bakar pada kelinci. Pembuatan gel dilakukan pada beberapa konsentrasi ekstrak daun pohpohan, yaitu 0,5, 1, dan 2%, Setelah dilakukan pembuatan gel, maka dilakukan evaluasi gel untuk mengetahui kualitas sediaan, meliputi uji organoleptis, homogenitas, uji pH, uji daya sebar, uji daya lekat, dan uji aktivitas luka bakar. Uji aktivitas luka bakar dilakukan pada 3 ekor kelinci jantan dengan 5 sisi perlakuan pada tiap kelinci, dan diinduksi dengan logam panas dengan alat uji Rafiky’sTool Pressure Test. Sebagai pembanding digunakan Bioplacenton®. Pemberian gel pada pengobatan luka bakar dilakukan 2 kali sehari. Parameter yang diukur adalah diameter luka bakar selama 10 hari. Data hasil uji aktivitas luka bakar diuji statistik menggunakan uji non parametric Kruskal-Wallis dan uji lanjut Mann Whitney. Hasil evaluasi gel menunjukkan bahwa gel yang mengandung ekstrak daun pohpohan konsentrasi 0,5, 1, dan 2% memenuhi persyaratan homogenitas, pH, daya penyebaran dan daya lekat. Hasil uji aktivitas luka bakar menunjukkan bahwa gel dengan konsentrasi ekstrak pohpohan 0,5, 1, dan 2% mampu menurunkan diameter luka bakar lebih cepat dibandingkan kontrol. Kelompok ekstrak daun pohpohan 2% memiliki aktivitas penyembuhan luka bakar paling cepat dan hampir sama dengan kontrol positif Bioplacenton®. Dapat disimpulkan bahwa gel yang mengandung ekstrak pohpohan mempunyai aktivitas menyembuhkan luka bakar. Kata kunci: Daun pohpohan (Pilea trinervia W.), Gel, Luka bakar Wound healing activity of pohpohan (Pilea trinervia W.) extract gel on rabbit Abstract Indonesian people recognize and utilize nutritious crops as one of the efforts in the prevention of health problems. One of the nutritious plants to heal burns is the leaves of pohpohan (Pilea trinervia W.). This study aims to test the activity of pohpohan leaf extract (Pilea trinervia W.) on the healing of burns in rabbits. Preparation of gel is done on some concentration of pohpohan leaf extract, that is 0,5, 1, and 2%. After gel making, gel evaluation is done to know the quality of preparation, including organoleptic test, homogeneity, pH test, spreading test, sticky, and burn activity test. Burning activity test was performed on 3 male rabbits with 5 treatment sides on each rabbit, and induced with hot metal by Rafiky'sTool Pressure Test. For comparison use Bioplacenton®. Gels on the treatment of burns done 2 times a day. Parameters measured were burn diameter for 10 days. Data on burn activity test results were tested statistically using non-parametric Kruskal-Wallis test and further test of Mann Whitney. The results of gel evaluation showed that the gel containing pohpohan leaves extract concentration of 0.5, 1, and 2% fulfilled the homogeneity, pH, dispersion and stickiness requirements. The result of burn activity test showed that gel with concentration of 0,5, 1, and 2% extract pohpohan able to reduce burn burn diameter faster than control. The 2% pohpohan leaf extract group has the fastest burn healing activity and almost equal to the positive control of Bioplacenton®. It can be concluded that gel containing pohpohan extract has activity to heal burns. Keywords: Pohpohan (Pilea trinervia W.), Gel, Wound healing

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Antimicrobial and Wound Healing Activity of Ixora coccinea Leaf Extract in Hydrogel Formulation

Acta Medica Philippina ◽

10.47895/amp.vi0.1975 ◽

2021 ◽

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Hydrogel Formulation ◽

Ixora Coccinea ◽

Wound Healing Activity

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Uji Perbandingan Aktivitas Penyembuhan Luka Sayat Fraksi n-Heksan, Metanol, dan Ekstrak Daun Talas (Colocasia esculenta (L.) Schott)

Prosiding Seminar Nasional Kesehatan ◽

10.48144/prosiding.v1i.714 ◽

2021 ◽

Vol 1 ◽

pp. 562-569

Author(s):

Dita Maharani ◽

W Wirasti ◽

S Slamet ◽

Urmatul Waznah

Keyword(s):

Wound Healing ◽

New Zealand ◽

Leaf Extract ◽

Treatment Method ◽

Negative Control ◽

Hexane Fraction ◽

Anova Analysis ◽

Methanol Fraction ◽

Wound Healing Activity ◽

New Zealand Rabbits

AbstractTalas or taro leaf contains active compounds in the form of flavonoids and saponins that can accelerate the process of epidermal tissue re-epitalization and infiltration of inflammatory cells in the wound area. The purpose of this study was to compare the healing activities of the n-hexane fraction, methanol fraction, and taro leaf extract in New Zealand rabbits. The extraction method using maceration, the fractionation method used liquid-liquid partition with a separating funnel and the treatment method on test animals used was the application of the n-hexane fraction, methanol fraction, and taro leaf extract on open wounds of New Zealand rabbits with positive control and negative control. The data obtained is in the form of the value of open wound narrowing on the rabbit’s back. The result of the data were analyzed by ANOVA (Analysis Of Variant) followed by the LSD (Least Significant Different) test to see which treatment gave different effect in each group. The results of the research or testing for 14 days showed that the extract, n-hexane fraction, and methanol fraction had wound healing activity on the rabbit back with statistical data showing significant differences between treatment groups. The extract had the most optimal wound healing activity compared to other treatment groups.Keywords: talas leaf, extract, fraction, wound AbstrakDaun talas mengandung senyawa aktif berupa flavonoid dan saponin yang mampu mempercepat proses reepitalisasi jaringan epidermis dan infiltrasi sel-sel radang pada daerah luka. Tujuan dari penelitian ini yaitu mengetahui perbandingan aktivitas penyembuhan luka terbuka fraksi n-heksan, fraksi metanol, dan ekstrak daun talas pada kelinci New Zealand. Metode ekstraksi menggunakan maserasi, metode fraksinasi menggunakan partisi cair-cair dengan corong pisah dan metode perlakuan pada hewan uji yang digunakan adalah pengolesan fraksi n-heksan, fraksi metanol, dan ekstrak daun talas pada luka terbuka kelinci New Zealand dengan kontrol positif dan kontrol negatif. Data yang didapatkan adalah berupa nilai penyempitan luka terbuka pada punggung kelinci. Hasil data dianalisis dengan ANOVA(Analysis Of Variant) yang dilanjutkan dengan uji LSD (Least Significant Different) untuk melihat perlakuan mana yang memberikan efek yang berbeda ditiap kelompok. Hasil penelitian atau pengujian selama 14 hari menunjukkan bahwa ekstrak, fraksi metanol, dan fraksi n-heksan mempunyai aktivitas penyembuhan luka pada punggung kelinci dengan data statistik yang menunjukkan adanya perbedaan signifikan antar kelompok perlakuan. Ekstrak mempunyai aktivitas daya sembuh luka yang paling optimal dibandingkan dengan kelompok perlakuan lain.Kata kunci : daun talas; ekstrak; fraksi; luka

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WOUND HEALING ACTIVITY OF THE LEAVES OF WRIGHTIA TINCTORIA (ROXB) R. BR

INDIAN DRUGS ◽

10.53879/id.49.03.p0040 ◽

2012 ◽

Vol 49 (03) ◽

pp. 40-46

Author(s):

M. C. Divakar ◽

◽

S Lakshmi Devi

Keyword(s):

Wound Healing ◽

Tensile Strength ◽

Wistar Rats ◽

Leaf Extract ◽

Excision Wound ◽

Wound Model ◽

Methanolic Extracts ◽

Wrightia Tinctoria ◽

Incision Wound ◽

Wound Healing Activity

The study was aimed to evaluate wound healing activity of 70% ethanolic and methanolic extracts of leaves of Wrightia tinctoria (Roxb) R. Br (Apocynaceae) using incision and excision wound models on Wistar rats. Wound contraction and period of epithelization were assessed in excision wound model whereas wound tensile strength was determined in case of incision wound model. 4% Wrightia tinctoria methanolic leaf extract phytosome exhibited significant wound healing potential when compared with standard 0.2% nitrofurazone ointment.

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AN in vitro STUDY OF WOUND HEALING ACTIVITY OF Ficus deltoidea LEAF EXTRACT

Jurnal Teknologi ◽

10.11113/jt.v77.6008 ◽

2015 ◽

Vol 77 (3) ◽

Author(s):

Nurul Ain Aqilla Wan Mustaffa ◽

Rosnani Hasham ◽

Mohamad Roji Sarmidi

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Wound Closure ◽

In Vitro Study ◽

Human Skin Fibroblast ◽

Dependent Manner ◽

Ficus Deltoidea ◽

Migration Assay ◽

Wound Healing Activity

Ficus deltoidea (Mas Cotek) is one of the popular herbs that has been used traditionally to alleviate illnesses. The present study aimed to investigate the in vitro wound healing activity of F. deltoidea leaf extract on skin cell. Cell proliferative and migration assay were done on the Human Skin Fibroblast cell (HSF 1184) which were treated with different concentrations of F. deltoidea leaf extract. The data of wound closure were collected at time intervals of 0, 6, 12 and 24 hours and analyzed using ImageJ™ software. MTT assay revealed that the F. deltoidea leaf extract could induce cell proliferation at a dose dependent manner. F. deltoidea leaf extract significantly accelerated the wound closure process in comparison to cells treated with ascorbic acid and untreated cells in scratch assay. Following 24 hours of incubation, cells treated with 50 mg/mL of F. deltoidea leaf extract showed remarkable proliferative and wound closure effect with 143.67% and 5.96%, respectively as compared to other concentrations. Collectively, these findings suggested that F. deltoidea leaf extract possesses wound healing potential and may be useful for the development of efficient wound healing drug.

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