scholarly journals Uji Perbandingan Aktivitas Penyembuhan Luka Sayat Fraksi n-Heksan, Metanol, dan Ekstrak Daun Talas (Colocasia esculenta (L.) Schott)

2021 ◽  
Vol 1 ◽  
pp. 562-569
Author(s):  
Dita Maharani ◽  
W Wirasti ◽  
S Slamet ◽  
Urmatul Waznah
Keyword(s):  
Wound Healing ◽  
New Zealand ◽  
Leaf Extract ◽  
Treatment Method ◽  
Negative Control ◽  
Hexane Fraction ◽  
Anova Analysis ◽  
Methanol Fraction ◽  
Wound Healing Activity ◽  
New Zealand Rabbits

AbstractTalas or taro leaf contains active compounds in the form of flavonoids and saponins that can accelerate the process of epidermal tissue re-epitalization and infiltration of inflammatory cells in the wound area. The purpose of this study was to compare the healing activities of the n-hexane fraction, methanol fraction, and taro leaf extract in New Zealand rabbits. The extraction method using maceration, the fractionation method used liquid-liquid partition with a separating funnel and the treatment method on test animals used was the application of the n-hexane fraction, methanol fraction, and taro leaf extract on open wounds of New Zealand rabbits with positive control and negative control. The data obtained is in the form of the value of open wound narrowing on the rabbit’s back. The result of the data were analyzed by ANOVA (Analysis Of Variant) followed by the LSD (Least Significant Different) test to see which treatment gave different effect in each group. The results of the research or testing for 14 days showed that the extract, n-hexane fraction, and methanol fraction had wound healing activity on the rabbit back with statistical data showing significant differences between treatment groups. The extract had the most optimal wound healing activity compared to other treatment groups.Keywords: talas leaf, extract, fraction, wound AbstrakDaun talas mengandung senyawa aktif berupa flavonoid dan saponin yang mampu mempercepat proses reepitalisasi jaringan epidermis dan infiltrasi sel-sel radang pada daerah luka. Tujuan dari penelitian ini yaitu mengetahui perbandingan aktivitas penyembuhan luka terbuka fraksi n-heksan, fraksi metanol, dan ekstrak daun talas pada kelinci New Zealand. Metode ekstraksi menggunakan maserasi, metode fraksinasi menggunakan partisi cair-cair dengan corong pisah dan metode perlakuan pada hewan uji yang digunakan adalah pengolesan fraksi n-heksan, fraksi metanol, dan ekstrak daun talas pada luka terbuka kelinci New Zealand dengan kontrol positif dan kontrol negatif. Data yang didapatkan adalah berupa nilai penyempitan luka terbuka pada punggung kelinci. Hasil data dianalisis dengan ANOVA(Analysis Of Variant) yang dilanjutkan dengan uji LSD (Least Significant Different) untuk melihat perlakuan mana yang memberikan efek yang berbeda ditiap kelompok. Hasil penelitian atau pengujian selama 14 hari menunjukkan bahwa ekstrak, fraksi metanol, dan fraksi n-heksan mempunyai aktivitas penyembuhan luka pada punggung kelinci dengan data statistik yang menunjukkan adanya perbedaan signifikan antar kelompok perlakuan. Ekstrak mempunyai aktivitas daya sembuh luka yang paling optimal dibandingkan dengan kelompok perlakuan lain.Kata kunci : daun talas; ekstrak; fraksi; luka

Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity
Keyword(s):  
New Zealand ◽  
Intramuscular Injection ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Blood And Urine Samples ◽  
Group 2 ◽  
New Zealand Rabbits ◽  
Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

scholarly journals Promotion of Dental Pulp Wound Healing in New Zealand White Rabbits’ Teeth by Thai Propolis Product

2018 ◽  
Vol 36 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Nattriya Likitpongpipat ◽  
Somboon Sangmaneedet ◽  
Poramaporn Klanrit ◽  
Rajda Noisombut ◽  
Suttichai Krisanaprakornkit ◽  
...  
Keyword(s):  
Wound Healing ◽  
New Zealand ◽  
Calcium Hydroxide ◽  
Median Number ◽  
Positive Control ◽  
Negative Control ◽  
Histological Evaluation ◽  
Dentinal Tubules ◽  
Pulp Capping ◽  
New Zealand White Rabbits

This study examined and compared wound healing between Thai propolis product and calcium hydroxide paste as pulp-capping agents after partial pulpotomy in New Zealand white rabbits. Forty incisor teeth from 10 rabbits were treated. Thirty-six teeth received class V cavity preparations with partial pulpotomy and application of either propolis or calcium hydroxide paste. Similar cavity preparations were performed in 2 teeth without any capping material as a positive control, whereas 2 teeth without the cavity preparation served as a negative control. Histological evaluation showed that both groups had dentin bridge formation. Dentinal tubules in the dentin bridge were more orderly arranged in the Thai propolis group than in the calcium hydroxide group. Wound healing and the median number of hyperemic blood vessels were not statistically significant different between the 2 groups. Thai propolis product may be used as a pulp-capping agent.

Phytochemical Analysis and Wound-Healing Activity of Noni (Morinda Citrifolia) Leaf Extract

2020 ◽  
Vol 26 (4) ◽  
pp. 379-393
Author(s):  
Hai Trieu Ly ◽  
Minh Trang Pham Nguyen ◽  
Thi Kim Oanh Nguyen ◽  
Thi Phuong Quynh Bui ◽  
Xu Ke ◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Morinda Citrifolia ◽  
Phytochemical Analysis ◽  
Wound Healing Activity

scholarly journals Preparation, Characterization, and Wound Healing Activity of Papaya Leaves Extract on Spray Gel

2020 ◽  
Vol 25 (2) ◽  
pp. 103
Author(s):  
Dina Permata Wijaya ◽  
Herlina Herlina ◽  
Najma Annuria Fitri ◽  
Mardiyanto Mardiyanto ◽  
Mustikasanti Mustikasanti ◽  
...  
Keyword(s):  
Wound Healing ◽  
Positive Control ◽  
Negative Control ◽  
Gellan Gum ◽  
Gelling Agent ◽  
Carbopol 940 ◽  
Ph Range ◽  
Wound Healing Activity

Papaya leaves have been using for wound healing that contains flavonoids, saponins, phenolics, chymopapain, and papain enzymes. The aim of this research were preparation, characterization, and wound healing activity of papaya leaves extract on spray gel. Spray gel was formulated with variation of gelling agent such as carbopol 940, HPMC, gellan gum, and hydroxyethylcellulose. The spray gel were characterizated by organoleptic, pH, stickiness test, viscosity, homogeneity, weight, and wound healing activity in rats. The results showed were all of formula spray gel have brown and homogeneous, pH between 5,947-6,347 within pH range of skin, stickiness test between 1,92-8,12 s, viscosity between 880-1740 cPs. Papaya leaves extract on spray gel has wound healing activity in rats faster than extract and positive control that is 16 days. The wound healing of papaya leaves extract on spray gel exhibited significantly different (p<0,05) than negative control.

scholarly journals Burn Wound Healing Activity of Ethanolic Extract of Acalypha indica in Oinment Formulated against Rabbits (Ocyctagus caniculus)

2020 ◽  
Vol 8 (3) ◽  
pp. 18-20
Author(s):  
Khairani Fitri ◽  
Tetty Noverita Khairani ◽  
Fajar Apollo Sinaga ◽  
Amenia Gracia Talunohi
Keyword(s):  
Wound Healing ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Burn Wound ◽  
Leaf Extracts ◽  
Laboratory Design ◽  
Burn Wound Healing ◽  
Healing Phase ◽  
Wound Healing Activity ◽  
Acalypha Indica

Objectives: The purpose of this study was to determine burn wound healing activity of Acalypha indica L. in oinment formulated. Design: This study uses an experimental laboratory design. This study used rabbits as test animals induced by burns with hot iron plates on the back. Extract ointment applied to the wound then observed the development of healing. Interventions: The extract was formulated in ointments with concentrations of 3, 5 and 7%. positive control used was ointment containing sesame oil and negative control used is an ointment base. Main outcome measure: The results showed a concentration of 3% had a slow and low effect, at a concentration of 5% it had a moderate healing phase, and a concentration of 7% had a good healing phase. Conclusion: The ointments containing Acalypha indica leaf extracts have good activity in healing burns in rabbits.    

scholarly journals Uji aktivitas gel ekstrak daun pohpohan (Pilea trinervia W.) terhadap penyembuhan luka bakar pada kelinci (Oryctolagus cuniculus)

2017 ◽  
Vol 5 (2) ◽  
pp. 75
Author(s):  
Via Fitria ◽  
Rafiki Fahrul Arifin ◽  
Nia Kurniasih
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Positive Control ◽  
Test Results ◽  
Activity Test ◽  
Pressure Test ◽  
Spreading Test ◽  
Wound Healing Activity ◽  
Non Parametric

<p align="center"><strong>Abstrak</strong></p><p align="center"><strong> </strong></p><p>Masyarakat Indonesia mengenal dan memanfaatkan tanaman berkhasiat obat sebagai salah satu upaya dalam penanggulangan masalah kesehatan. Salah satu tanaman berkhasiat untuk menyembuhkan luka bakar adalah daun pohpohan (<em>Pilea trinervia </em>W.). Penelitian ini bertujuan menguji aktivitas gel ekstrak daun pohpohan (<em>Pilea trinervia W</em>.) terhadap penyembuhan luka bakar pada kelinci. Pembuatan gel dilakukan pada beberapa konsentrasi ekstrak daun pohpohan, yaitu 0,5, 1, dan 2%, Setelah dilakukan pembuatan gel, maka dilakukan evaluasi gel untuk mengetahui kualitas sediaan, meliputi uji organoleptis, homogenitas, uji pH, uji daya sebar, uji daya lekat, dan uji aktivitas luka bakar. Uji aktivitas luka bakar dilakukan pada 3 ekor kelinci jantan dengan 5 sisi perlakuan pada tiap kelinci, dan diinduksi dengan logam panas dengan alat uji Rafiky’sTool Pressure Test. Sebagai pembanding digunakan Bioplacenton®. Pemberian gel pada pengobatan luka bakar dilakukan 2 kali sehari. Parameter yang diukur adalah diameter luka bakar selama 10 hari. Data hasil uji aktivitas luka bakar diuji statistik menggunakan uji non parametric Kruskal-Wallis dan uji lanjut Mann Whitney. Hasil evaluasi gel menunjukkan bahwa gel yang mengandung ekstrak daun pohpohan konsentrasi 0,5, 1, dan 2% memenuhi persyaratan homogenitas, pH, daya penyebaran dan daya lekat. Hasil uji aktivitas luka bakar menunjukkan bahwa gel dengan konsentrasi ekstrak pohpohan 0,5, 1, dan 2% mampu menurunkan diameter luka bakar lebih cepat dibandingkan kontrol. Kelompok ekstrak daun pohpohan 2% memiliki aktivitas penyembuhan luka bakar paling cepat dan hampir sama dengan kontrol positif Bioplacenton®. Dapat disimpulkan bahwa gel yang mengandung ekstrak pohpohan mempunyai aktivitas menyembuhkan luka bakar.</p><p> </p><p><strong>Kata kunci:</strong>     Daun pohpohan (<em>Pilea trinervia</em> W.), Gel, Luka bakar</p><p> </p><p align="center"><strong><em>Wound healing activity of pohpohan </em></strong><strong>(<em>Pilea trinervia </em>W.) </strong><strong><em>extract gel on rabbit</em></strong></p><p align="center"><strong><em> </em></strong></p><p align="center"><strong><em>Abstract</em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Indonesian people recognize and utilize nutritious crops as one of the efforts in the prevention of health problems. One of the nutritious plants to heal burns is the leaves of pohpohan (Pilea trinervia W.). This study aims to test the activity of pohpohan leaf extract (Pilea trinervia W.) on the healing of burns in rabbits. Preparation of gel is done on some concentration of pohpohan leaf extract, that is 0,5, 1, and 2%. After gel making, gel evaluation is done to know the quality of preparation, including organoleptic test, homogeneity, pH test, spreading test, sticky, and burn activity test. Burning activity test was performed on 3 male rabbits with 5 treatment sides on each rabbit, and induced with hot metal by Rafiky'sTool Pressure Test. For comparison use Bioplacenton®. Gels on the treatment of burns done 2 times a day. Parameters measured were burn diameter for 10 days. Data on burn activity test results were tested statistically using non-parametric Kruskal-Wallis test and further test of Mann Whitney. The results of gel evaluation showed that the gel containing pohpohan leaves extract concentration of 0.5, 1, and 2% fulfilled the homogeneity, pH, dispersion and stickiness requirements. The result of burn activity test showed that gel with concentration of 0,5, 1, and 2% extract pohpohan able to reduce burn burn diameter faster than control. The 2% pohpohan leaf extract group has the fastest burn healing activity and almost equal to the positive control of Bioplacenton®. It can be concluded that gel containing pohpohan extract has activity to heal burns.</em></p><p><em> </em></p><p><strong><em>Keywords:</em></strong><em>  P</em><em>ohpohan (Pilea trinervia W.), Gel, Wound healing</em></p>

scholarly journals LETHAL ENCEPHALITOZOONOSIS IN CYCLOPHOSPHAMIDE-TREATED RABBITS

1999 ◽  
Vol 47 (1) ◽  
pp. 85-93 ◽  
Author(s):  
L'. Leng ◽  
M. Štefkovič ◽  
Viera Révajová ◽  
Halanová ◽  
M. Horváth
Keyword(s):  
New Zealand ◽  
Clinical Signs ◽  
Experimental Period ◽  
Peripheral Blood Cell ◽  
Negative Control ◽  
Blood Cell Count ◽  
Clinical Form ◽  
Experimental Animals ◽  
New Zealand Rabbits ◽  
Positive Controls

Encephalitozoonosis is an opportunistic infection in animals and humans. Its clinical form is observed in immunosuppressed hosts. We studied the occurrence of the manifest form of rabbit microsporidiosis under cyclophosphamide immunomodulation in 40 New Zealand rabbits. The experimental animals were intraperitoneally infected with 5 Ã 107Encephalitozoon cuniculispores. Two weeks after infection the animals were treated intraperitoneally with cyclophosphamide, first with 50 mg/kg and then with 15 mg/kg weekly during the 12-week experimental period. Positive controls were eitherE. cuniculi-infected or cyclophosphamide-immunosuppressed animals. The negative control rabbits remained untreated. Both clinical signs of encephalitozoonosis and depression of peripheral blood cell count developed between weeks 4 and 6 in the experimental animals which died during week 6 of the experiment. No clinical signs compatible with encephalitozoonosis were observed in any of the controls. The results suggest that immunosuppression induced by cyclophosphamide can give rise to a lethal form of encephalitozoonosis.

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