Agronomic evaluation of seedlings from crosses between the main Spanish olive cultivar ‘Picual’ and two wild olive trees

2014 ◽  
Vol 89 (5) ◽  
pp. 508-512 ◽  
Author(s):  
Tatjana Klepo ◽  
Amani Toumi ◽  
RaÚl De La Rosa ◽  
Lorenzo LeÓn ◽  
Angjelina Belaj
Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1304
Author(s):  
Francisco Espínola ◽  
Alfonso M. Vidal ◽  
Juan M. Espínola ◽  
Manuel Moya

Wild olive trees have important potential, but, to date, the oil from wild olives has not been studied significantly, especially from an analytical point of view. In Spain, the wild olive tree is called “Acebuche” and its fruit “Acebuchina”. The objective of this work is to optimize the olive oil production process from the Acebuchina cultivar and characterize the oil, which could be marketed as healthy and functional food. A Box–Behnken experimental design with five central points was used, along with the Response Surface Methodology to obtain a mathematical experimental model. The oils from the Acebuchina cultivar meet the requirements for human consumption and have a good balance of fatty acids. In addition, the oils are rich in antioxidants and volatile compounds. The highest extraction yield, 12.0 g oil/100 g paste, was obtained at 90.0 min and the highest yield of phenolic compounds, 870.0 mg/kg, was achieved at 40.0 °C, and 90.0 min; but the maximum content of volatile compounds, 26.9 mg/kg, was obtained at 20 °C and 30.0 min. The oil yield is lower than that of commercial cultivars, but the contents of volatile and phenolic compounds is higher.


1985 ◽  
Vol 35 ◽  
pp. 93-97 ◽  
Author(s):  
Stephen Hill

The ruins at Yanıkhan form the remains of a Late Roman village in the interior of Rough Cilicia some 8 kilometres inland from the village of Limonlu on the road to Canbazlı (see Fig. 1). The site has not been frequently visited by scholars, and the first certain reference to its existence was made by the late Professor Michael Gough after his visit on 2 September 1959. Yanıkhan is now occupied only by the Yürüks who for years have wintered on the southern slopes of Sandal Dağ. The ancient settlement at Yanıkhan consisted of a village covering several acres. The remains are still extensive, and some, especially the North Basilica, are very well preserved, but there has been considerable disturbance in recent years as stone and rubble have been removed in order to create small arable clearings. The visible remains include many domestic buildings constructed both from polygonal masonry without mortar and from mortar and rubble with coursed smallstone facing. There are several underground cisterns and a range of olive presses. The countryside around the settlement has been terraced for agricultural purposes in antiquity, and is, like the settlement itself, densely covered with scrub oak and wild olive trees. The most impressive remains are those of the two basilical churches which are of little artistic pretension, but considerable architectural interest. The inscription which forms the substance of this article was found on the lintel block of the main west entrance of the South Basilica.


Plant Disease ◽  
2008 ◽  
Vol 92 (8) ◽  
pp. 1252-1252 ◽  
Author(s):  
J. Moral ◽  
R. De la Rosa ◽  
L. León ◽  
D. Barranco ◽  
T. J. Michailides ◽  
...  

Traditional olive orchards in Spain have been planted at a density of 70 to 80 trees per ha with three trunks per tree. During the last decade, the hedgerow orchard, in which planting density is approximately 2,000 trees per ha, was developed. In 2006 and 2007, we noted a severe outbreak of fruit rot in FS-17, a new cultivar from Italy, in an experimental hedgerow planting in Córdoba, southern Spain. The incidence of fruit rot in ‘FS-17’ was 80% in January of 2006 and 24% in January of 2007. Cvs. Arbosana, IRTA-i18 (a selected clone from ‘Arbequina’), and Koroneiki had no symptoms in either year of the study. Disease incidence in ‘Arbequina’ was <0.1% only in 2006. Affected fruits were soft with gray-white skin and they eventually mummified. Black-green sporodochia were observed on the surface of diseased fruits. A fungus was isolated from diseased fruits on potato dextrose agar (PDA) and incubated at 22 to 26°C with a 12-h photoperiod. After 8 days of growing on PDA, fungal colonies formed conidial chains having a main axis with up to 10 conidia and secondary and tertiary short branches with two to four conidia. Conidia were obpyriform, ovoid, or ellipsoidal, without a beak or with a short beak, had up to four transverse septa, and measured 11.7 to 24.7 (mean 19.6) μm long and 7.7 to 13.0 (mean 9.6) μm wide at the broadest part of the conidium. The length of the beak of conidia was variable, ranging from 0 to 28.6 (mean 5.5) μm. The fungus was identified as Alternaria alternata (1). Pathogenicity tests were performed by spraying 40 mature fruits of ‘FS-17’ with a spore suspension (1 × 106 spores per ml). The same number of control fruits was treated with water. After 21 days, inoculated fruit developed symptoms that had earlier been observed in the field. A. alternata was reisolated from lesions on all infected fruits. The fungus was not isolated from any of the control fruits. The experiment was performed twice. The new growing system and the high susceptibility of some olive cultivars, such as FS-17, may result in a high incidence of disease caused by a pathogen that is generally characterized as weakly virulent. To our knowledge, this is the first report of A. alternata causing a severe outbreak of fruit rot on olive trees in the field. References: (1) B. M. Pryor and T. J. Michailides. Phytopathology 92:406, 2002.


Pathogens ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 35 ◽  
Author(s):  
Marzia Vergine ◽  
Joana B. Meyer ◽  
Massimiliano Cardinale ◽  
Erika Sabella ◽  
Martin Hartmann ◽  
...  

Xylella fastidiosa is a highly virulent pathogen that causes Olive Quick Decline Syndrome (OQDS), which is currently devastating olive plantations in the Salento region (Apulia, Southern Italy). We explored the microbiome associated with X. fastidiosa-infected (Xf-infected) and -uninfected (Xf-uninfected) olive trees in Salento, to assess the level of dysbiosis and to get first insights into the potential role of microbial endophytes in protecting the host from the disease. The resistant cultivar “Leccino” was compared to the susceptible cultivar “Cellina di Nardò”, in order to identify microbial taxa and parameters potentially involved in resistance mechanisms. Metabarcoding of 16S rRNA genes and fungal ITS2 was used to characterize both total and endophytic microbiota in olive branches and leaves. “Cellina di Nardò” showed a drastic dysbiosis after X. fastidiosa infection, while “Leccino” (both infected and uninfected) maintained a similar microbiota. The genus Pseudomonas dominated all “Leccino” and Xf-uninfected “Cellina di Nardò” trees, whereas Ammoniphilus prevailed in Xf-infected “Cellina di Nardò”. Diversity of microbiota in Xf-uninfected “Leccino” was higher than in Xf-uninfected “Cellina di Nardò”. Several bacterial taxa specifically associated with “Leccino” showed potential interactions with X. fastidiosa. The maintenance of a healthy microbiota with higher diversity and the presence of cultivar-specific microbes might support the resistance of “Leccino” to X. fastidiosa. Such beneficial bacteria might be isolated in the future for biological treatment of the OQDS.


Plant Disease ◽  
2012 ◽  
Vol 96 (9) ◽  
pp. 1378-1378 ◽  
Author(s):  
J. R. Úrbez-Torres ◽  
F. Peduto ◽  
W. D. Gubler

The California olive industry produces 99% of the U.S. olive crop, which represented a value of over $113 million in 2010. During the 2008 and 2009 growing seasons, decline of young super-high-density olive cvs. Arbequina, Arbosana, and Koroneiki trees (<4 years old) was observed in orchards throughout Glenn, Yolo, and San Joaquin Counties. Symptomatic trees showed stunted growth and chlorotic leaves with roots having black, sunken, necrotic lesions, which frequently prolonged into the base and crown of the tree. Twenty-five trees were collected from different orchards and necrotic roots as well as infected trunk tissue were plated onto potato dextrose agar amended with 0.01% tetracycline hydrochloride. Cultures were incubated at room temperature (23 ± 2°C) until fungal colonies were observed. In 17 out of 25 trees collected (68%), light yellow fungal colonies were observed from the symptomatic tissue after 7 to 10 days. Colonies turned dark yellow to orange with age and showed an orange-dark brown reverse. Both microconidia (hyaline, ellipsoidal to ovoidal and aseptate (n = 60) (6.5) 11.5 to 13.5 (17.1) × (3) 3.4 to 4.5 (5.6) μm) and macroconidia (hyaline, cylindrical, straight and/or slightly curved with one, two or three septa (n = 60) (12.5) 26.5 to 38.5 (44.1) × (4) 5.5 to 7.5 (8.5) μm) were observed. Culture and conidial morphology were in concordance with previous published description of Ilyonectria macrodidyma (Halleen, Schroers & Crous) P. Chaverri & C. Salgado (1,3,4). Identification to species level was confirmed by sequence comparison of four Californian isolates (UCCE958, UCCE959, UCCE960, and UCCE961) with sequences available in GenBank using the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rDNA (primers ITS1/ITS4), a portion of the β-tubulin gene (BT1a/BT1b), and a partial sequence of the mitochondrial small subunit rDNA (NMS1/NMS2) (4). Fungal sequences of isolates from olive from California (GenBank JQ868543 to JQ868554) showed 99 to 100% homology with previously identified and deposited I. macrodidyma isolates in Genbank for all three genes. Pathogenicity of I. macrodidyma in olive cvs. Arbequina, Arbosan, and Koroneiki was investigated using two fungal isolates (UCCE958 and UCCE960) as reported by Petit and Gubler (4). The roots of 10 1-year-old trees per fungal isolate for each olive cultivar were individually inoculated with 25 ml of a 106 conidia/ml spore suspension and placed in a lath house at the UC Davis field station. Additionally, 10 trees per cultivar were inoculated with sterile water as controls. Six months after inoculation, most of the inoculated olive plants showed chlorotic leaves similar to those observed in commercial orchards. Root necrosis for each cv. was expressed as the percentage of root length having lesions (2). No significant difference was observed between isolates and average root necrosis was 29.4, 35.6, and 38.3% in Koroniki, Arbosana, and Arbequina, respectiveley. I. macrodidyma was recovered from symptomatic roots in each of the cvs. and identified based on morphology. No root rot symptoms were observed in the controls. To our knowledge, this is the first report of I. macrodidyma causing root rot of olive trees not only in California but anywhere in the world. References: (1) P. Chaverri et al. Stud. Mycol. 68:57, 2011. (2) M. Giovanetti and B. Mosse. New Phytol. 84:489, 1980. (3) F. Halleen et al. Stud. Mycol. 50:421, 2004. (4) E. Petit and W. D. Gubler. Plant Dis. 89:1051, 2005.


2019 ◽  
Vol 20 (3) ◽  
pp. 350-370 ◽  
Author(s):  
Hédia Hannachi ◽  
Walid Elfalleh ◽  
Majda Laajel ◽  
Imen Ennajeh ◽  
Ridha Fethi Mechlouch ◽  
...  

2020 ◽  
Vol 41 (2) ◽  
pp. 163-172
Author(s):  
Hédia Hannachi ◽  
Mohamed Ali Benabderrahim ◽  
Walid Elfalleh ◽  
Rongchun Wang ◽  
Ma Ying

Few studies have reported the relationship between wild (Olea europaea L. subsp. europaea var. sylvestris) and cultivated (Olea europaea L. subsp. europaea var. europaea) olive trees by using diverse markers. Herein, the amino and fatty acids composition of stones from wild and cultivated olives were assessed respectively using amino acids analyzer and gas chromatography coupled with mass spectrometry. Stones of 24 Tunisian olive samples including twelve cultivated trees and twelve wild trees were obtained from olives harvested at ripe stage. Results showed that 17 amino acids (with eight essential amino acids) and 15 fatty acids (eight saturated and seven unsaturated) were detected in the both olive taxa. Statistically, significant differences among wild and cultivated stones were observed for amino and fatty acids contents. Based on the major fatty acids and the essential amino acids, multivariate analyses classified olive varieties into three groups showing a close relationship between some wild and cultivated olive trees. Results were useful to distinguish some interest wild olive genotypes having stones richer in essential amino acids and monounsaturated fatty acids. Wild olive trees would constitute a genetic pool of interest criteria. These data would be used as complementary tool to morphological traits and molecular markers studies providing a relationship between the cultivated and wild olive trees.


2013 ◽  
Vol 16 (6) ◽  
pp. 1271-1283 ◽  
Author(s):  
Hédia Hannachi ◽  
Nizar Nasri ◽  
Walid Elfalleh ◽  
Nizar Tlili ◽  
Ali Ferchichi ◽  
...  

2013 ◽  
Vol 54 (2) ◽  
pp. 1965-1971 ◽  
Author(s):  
Pervez Anwar ◽  
Alessandra Bendini ◽  
Muhammad Gulfraz ◽  
Rahmatullah Qureshi ◽  
Enrico Valli ◽  
...  
Keyword(s):  

Plant Disease ◽  
2005 ◽  
Vol 89 (2) ◽  
pp. 208-208 ◽  
Author(s):  
M. A. Romero ◽  
M. E. Sánchez ◽  
A. Trapero

Botryosphaeria spp. are known to produce cankers and dieback in several woody hosts. Since 2001, a severe disease resulting in the dieback of branches was observed in olive (Olea europaea) orchards in Andalucía, southern Spain, where the only cultivar affected was Gordal de Sevilla. The affected trees showed abundant dead twigs, and wilted leaves remained attached. These symptoms were similar to those caused by Resseliella oleisuga, a bark borer insect common in the region. Closer inspection showed no evidence of insects, but revealed reddish brown bark lesions that girdled the affected branches. When the outer bark was removed, the affected tissue appeared dark brown, in contrast to the yellowish green of healthy inner bark. A Fusicoccum sp. was consistently isolated from the necrotic bark. Conidia produced in black pycnidia were hyaline, aseptate, fusoid, with a truncate base averaging 19.9 × 6.7 μm, becoming pale brown with all cells the same color, and developing one or two septa when germinated. These characteristics conform to Fusicoccum ribis (1), the anamorph of B. ribis. Pathogenicity of two isolates from symptomatic branches was determined by stem inoculations of 4-year-old cv. Gordal de Sevilla grafted on wild olive trees growing in plastic pots containing a sand/lime/peat soil mixture. Both isolates were inoculated by three techniques. In the first, 7-mm-diameter agar plugs bearing actively growing mycelium were applied to 7-mm-diameter bark wounds made with a cork borer on the middle point of stems (averaging 10 mm in diameter). In the second technique, the same type of inoculum was applied directly to the bark without any wounding. For the third technique, 100 μl of conidial suspension in water (2 × 105 conidia per ml) was applied to bark wounds. The inoculated stem sections were wrapped in Parafilm to retain moisture. All inoculated and control plants (only sterile agar medium or sterile water added) were kept in a greenhouse and watered as needed. There were three replicate plants per isolate and inoculation technique. After 3 months, all plants inoculated with mycelium applied to wounds showed girdling and apical death with browning and wilting of leaves above the inoculation point. In the treatments where mycelium was applied to unwounded bark or where conidial suspensions were applied to wounds, small bark cankers developed to 56 mm long, but neither girdling nor foliar wilting were observed. No symptoms developed in the control plants. There were no significant differences in pathogenicity between the two F. ribis isolates. F. ribis was recovered from necrotic bark from every inoculated plant. These results demonstrate that B. ribis is pathogenic on cv. Gordal de Sevilla olives, and they indicate that bark wounding favors lesion development but is not needed for infection. Reference: (1) B. Slippers et al. Mycologia 96:83, 2004.


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