Chemometric profile & antimicrobial activities of leaf extract of Calotropis procera and Calotropis gigantea

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

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Antimicrobial of nyirih (Xylocarpus granatum) against pathogens of tiger shrimp post-larvae

F1000Research ◽

10.12688/f1000research.16653.1 ◽

2019 ◽

Vol 8 ◽

pp. 63 ◽

Cited By ~ 2

Author(s):

Gina Saptiani ◽

A. Syafei Sidik ◽

Fikri Ardhani

Keyword(s):

Leaf Extract ◽

Vibrio Harveyi ◽

Antimicrobial Activities ◽

Coastal Communities ◽

Distilled Water ◽

Leaf Extracts ◽

Potential Source ◽

Xylocarpus Granatum ◽

Tiger Shrimp ◽

Negative Controls

Background: Xylocarpus granatum has been used as a medicinal plant by coastal communities, which may indicate that this plant is a potential source of pharmaceuticals. Methods: Xylocarpus granatum leaf extract was tested as an antimicrobial agent for pathogens infecting tiger shrimp post-larvae. Of the treatments applied to the post-larvae, 25 were crudely extracted with ethanol, distilled water, and seawater solvent given by immersion. Vibrio harveyi and Saprolegnia sp. were microbial species used for the test. Results: X. granatum extract had the potential to inhibit V. harveyi and Saprolegnia sp., reducing infection and improving the survival of shrimp. Shrimp soaked with X. granatum extract had a total Vibrio count ranging from 14.67x103 to 22.67x103 CFU/ml. The survival rate of shrimp was recorded as 53.33% to 78.67% and 54.67% to 76.00% due to V. harveyi, and Saprolegnia sp infection, respectively. The relative percentage of the survival of shrimp protected from V. harveyi and Saprolegnia sp infection in treatments compared to negative controls ranged from 40.61% to 72.89% and 35.84% to 66.12%, respectively. Conclusions: Leaf extracts of X. granatum, which might have better antimicrobial activities to prevent tiger shrimp from pathogenetic infection, were consecutively extracted ethanol at 800-1,000 ppm, distilled water at 800-1,000 ppm, and seawater at 1,000 ppm.

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Corrigendum to “Investigation of the Antidiarrheal and Antimicrobial Activities of 80% Methanolic Leaf Extract of Discopodium penninervum (Hochst.)”

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/4915802 ◽

2018 ◽

Vol 2018 ◽

pp. 1-1 ◽

Cited By ~ 1

Author(s):

Dagninet Derebe ◽

Mohammedbrhan Abdulwuhab ◽

Muluken Wubetu ◽

Faiz Mohammed

Keyword(s):

Leaf Extract ◽

Antimicrobial Activities

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Facile single step preparations of phyto-nanoparticles of iron in Calotropis procera leaf extract to evaluate their antifungal potential against Alternaria alternata

Current Plant Biology ◽

10.1016/j.cpb.2020.100157 ◽

2020 ◽

Vol 23 ◽

pp. 100157 ◽

Cited By ~ 1

Author(s):

Musrat Ali ◽

Urooj Haroon ◽

Maria Khizar ◽

Hassan Javed Chaudhary ◽

Muhammad Farooq Hussain Munis

Keyword(s):

Alternaria Alternata ◽

Leaf Extract ◽

Single Step ◽

Calotropis Procera ◽

Antifungal Potential

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Biosynthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from leaf extract of Glycosmis pentaphylla (Retz.) DC

Microbial Pathogenesis ◽

10.1016/j.micpath.2018.01.003 ◽

2018 ◽

Vol 116 ◽

pp. 44-48 ◽

Cited By ~ 34

Author(s):

S. Vijayakumar ◽

C. Krishnakumar ◽

P. Arulmozhi ◽

S. Mahadevan ◽

N. Parameswari

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Leaf Extract ◽

Antimicrobial Activities ◽

Oxide Nanoparticles

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Essential Oil of Calotropis procera: Comparative Chemical Profiles, Antimicrobial Activity, and Allelopathic Potential on Weeds

Molecules ◽

10.3390/molecules25215203 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5203

Author(s):

Saud L. Al-Rowaily ◽

Ahmed M. Abd-ElGawad ◽

Abdulaziz M. Assaeed ◽

Abdelbaset M. Elgamal ◽

Abd El-Nasser G. El Gendy ◽

...

Keyword(s):

Antimicrobial Activity ◽

Biological Activities ◽

Principal Component ◽

Secondary Compounds ◽

Antimicrobial Activities ◽

Climatic Conditions ◽

Calotropis Procera ◽

Bidens Pilosa ◽

Allelopathic Potential ◽

Chemical Profiles

Plants are considered green resources for thousands of bioactive compounds. Essential oils (EOs) are an important class of secondary compounds with various biological activities, including allelopathic and antimicrobial activities. Herein, the present study aimed to compare the chemical profiles of the EOs of the widely distributed medicinal plant Calotropis procera collected from Saudi Arabia and Egypt. In addition, this study also aimed to assess their allelopathic and antimicrobial activities. The EOs from Egyptian and Saudi ecospecies were extracted by hydrodistillation and analyzed via GC-MS. The correlation between the analyzed EOs and those published from Egypt, India, and Nigeria was assessed by principal component analysis (PCA) and agglomerative hierarchical clustering (AHC). The allelopathic activity of the extracted EOs was tested against two weeds (Bidens pilosa and Dactyloctenium aegyptium). Moreover, the EOs were tested for antimicrobial activity against seven bacterial and two fungal strains. Ninety compounds were identified from both ecospecies, where 76 compounds were recorded in Saudi ecospecies and 33 in the Egyptian one. Terpenes were recorded as the main components along with hydrocarbons, aromatics, and carotenoids. The sesquiterpenes (54.07%) were the most abundant component of EO of the Saudi sample, while the diterpenes (44.82%) represented the mains of the Egyptian one. Hinesol (13.50%), trans-chrysanthenyl acetate (12.33%), 1,4-trans-1,7-cis-acorenone (7.62%), phytol (8.73%), and myristicin (6.13%) were found as the major constituents of EO of the Saudi sample, while phytol (38.02%), n-docosane (6.86%), linoleic acid (6.36%), n-pentacosane (6.31%), and bicyclogermacrene (4.37%) represented the main compounds of the Egyptian one. It was evident that the EOs of both ecospecies had potent phytotoxic activity against the two tested weeds, while the EO of the Egyptian ecospecies was more effective, particularly on the weed D. aegyptium. Moreover, the EOs showed substantial antibacterial and antifungal activities. The present study revealed that the EOs of Egyptian and Saudi ecospecies were different in quality and quantity, which could be attributed to the variant environmental and climatic conditions. The EOs of both ecospecies showed significant allelopathic and antimicrobial activity; therefore, these EOs could be considered as potential green eco-friendly resources for weed and microbe control, considering that this plant is widely grown in arid habitats.

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