Effect of Tagetes minuta oil on larval morphology of Plutella xylostella through scanning electron microscopy and mechanism of action by enzyme assay

Toxin Reviews ◽  
2021 ◽  
pp. 1-8
Author(s):  
Shudh Kirti Dolma ◽  
C. S. Jayaram ◽  
Nandita Chauhan ◽  
S. G. Eswara Reddy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Mechanism Of Action ◽  
Plutella Xylostella ◽  
Enzyme Assay ◽  
Larval Morphology ◽  
Tagetes Minuta ◽  
Scanning Electron

scholarly journals Pathogenicity of Aspergillus westerdijkiae to females and oothecae of Periplaneta Americana

2015 ◽  
Vol 46 (1) ◽  
pp. 20-25 ◽  
Author(s):  
Mariah Valente Baggio ◽  
Marcelo da Costa Ferreira ◽  
Antonio Carlos Monteiro ◽  
Walter Maldonado Junior ◽  
Manoel Victor Franco Lemos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Mechanism Of Action ◽  
Entomopathogenic Fungi ◽  
Periplaneta Americana ◽  
Selective Pressure ◽  
Aspergillus Westerdijkiae ◽  
Scanning Electron ◽  
Methods Of Control

ABSTRACT: Cockroach control is performed by the application of chemical insecticides which exert high selective pressure on populations and introduces synthetic substances in the environment, motivating the search for other methods of control such as entomopathogenic fungi. The objectives of this study were to investigate the pathogenicity of the JAB 42 Aspergillus westerdijkiae to females and oothecae of Periplaneta americana and to demonstrate its mechanism of action on oothecae. Suspensions containing 106 to 108 conidia/ml were used to infect females and oothecae. Mortality and other variables such as scanning electron microscopy were used to demonstrate the mechanism of action of the fungus. The isolated JAB 42 A. westerdijkiae is pathogenic to oothecae of P. americana, with low capacity to kill females. Adhesion, germination, penetration and extrusion of the fungus on the cockroach oothecae were observed.

Testing species delimitation with larval morphology: scanning electron microscopy analysis of protonymphon larvae of two closely related sea spiders, Pallenopsis patagonica (Hoek) and Pallenopsis yepayekae Weis

2017 ◽  
Vol 31 (4) ◽  
pp. 363 ◽  
Author(s):  
Jeremy Hübner ◽  
Philipp Wagner ◽  
Tobias Lehmann ◽  
Roland R. Melzer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Imaging Techniques ◽  
Species Group ◽  
Larval Morphology ◽  
Close Relationship ◽  
The Status ◽  
Electron Microscopy Analysis ◽  
Species Specific ◽  
Scanning Electron

We used scanning electron microscopy (SEM) to establish species-specific sets of characters for protonymphon larvae of two representatives of the ‘patagonica’ species group of Pallenopsis, P. patagonica and P. yepayekae. The larvae of both species are ‘typical’ protonymphon larvae sensu Bain (2003). Despite the close relationship of the two species, we observed numerous features that allow for differential diagnosis, e.g. general habitus, the number, arrangement and branching type of setules, the armature of the movable and immovable chelifore fingers, and the shape of the dactylus and setules of appendages II and III. SEM is particularly suitable for visualising these features. Our results further support the idea that protonymphon larvae can be identified to species level when adequate imaging techniques are used, as is also the case for larvae of other arthropods. Moreover, the status of the two studied species of Pallenopsis is fully supported by protonymphon larval morphology.

Terminal-instar larval morphology and systematics of Eulophidae and Eupelmidae species (Hymenoptera, Chalcidoidea) parasitoids of gall wasps (Hymenoptera, Cynipidae) communities from Europe

2017 ◽  
Vol 48 (3) ◽  
pp. 257-284 ◽  
Author(s):  
Jose F. Gómez ◽  
Nieves-Aldrey Jose Luis
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Body Shape ◽  
Mediterranean Area ◽  
Head Capsule ◽  
Larval Morphology ◽  
Larval Body ◽  
Gall Wasps ◽  
First Time ◽  
Scanning Electron

As part of a wider study on the morphology and systematics of the terminal-instar larvae of Chalcidoidea parasitoids of gall wasps (Hym., Cynipidae) in Europe, a comparative study of the larval morphology of three species of Eulophidae, Aprostocetus eurytomae (Nees), Aulogymnus skianeuros (Ratzeburg) and Dichatomus acerinus Förster; and three species of Eupelmidae, Eupelmus aseculatus (Kalina), E. cerris Förster and E. microzonus Förster is presented. The studied taxa are key components of the communities associated with different galls on herbs, shrubs and oaks in different ecosystems of Mediterranean area. Main characters of interest found on the studied species were larval body shape, head capsule structure, mouthparts, mandibles and chaetotaxy pattern of body and head. Diagnostic characters of the families and species are described and illustrated for the first time with scanning electron microscopy (SEM). Information on the biology of each species is discussed and illustrated with photographs, including new rearing records.

scholarly journals Larval morphology, development and forensic importance ofSynthesiomyia nudiseta(Diptera: Muscidae) in Europe: a rare species or just overlooked?

2012 ◽  
Vol 103 (1) ◽  
pp. 98-110 ◽  
Author(s):  
Y. Velásquez ◽  
T. Ivorra ◽  
A. Grzywacz ◽  
A. Martínez-Sánchez ◽  
C. Magaña ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Life Cycle ◽  
Rare Species ◽  
Southern Europe ◽  
Larval Morphology ◽  
The World ◽  
Morphology Development ◽  
First Time ◽  
Scanning Electron

AbstractThe muscidSynthesiomyia nudiseta(van der Wulp, 1883) is a species with forensic importance in tropical and subtropical regions of the world. This fly has recently been introduced into southern Europe and, until now, had not been recorded in forensic cases in this region. Here, morphology of all larval instars ofS. nudisetais documented in detail by using a combination of light and scanning electron microscopy. Literature data concerning larval morphology are revised and characters allowing identification from other forensically important Muscidae are listed. The life cycle of this species was studied at four constant temperatures: 15, 20, 25 and 30 °C. Total development varied between 46.50 ± 0.97 days at 15 °C and 15.39 ± 0.32 days at 30 °C. Moreover, we report this species breeding in human corpses, for the first time in Europe, in forensic cases from autopsies at the Anatomical Forensic Institute of Madrid and the Institute of Legal Medicine of Alicante, Spain.

scholarly journals Description and illustration of the discotelic larvae of Pachyteles vignai Deuve, 1999 and P. digiulioi Deuve, 1999 from the Otonga cloud forest (Ecuador) (Coleoptera, Carabidae, Ozaenini)

2021 ◽  
pp. 47-81
Author(s):  
Andrea Di Giulio ◽  
Maurizio Muzzi ◽  
Simone Fattorini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nature Reserve ◽  
Cloud Forest ◽  
Image Acquisition ◽  
The Other ◽  
Larval Morphology ◽  
Diagnostic Characters ◽  
Scanning Electron

The peculiar morphology of the discotelic larvae of three species of Pachyteles (Coleoptera, Carabidae, Paussinae, ozaenini) collected in an Andean cloud forest in Ecuador (otonga nature Reserve, Cotopaxi) is here analyzed. All instars of Pachyteles vignai Deuve, 1999, 2nd and 3rd instars of P. digiulioi Deuve, 1999, and the 3rd instar of another undetermined Pachyteles species are described. A particular emphasis on the illustration of the aberrant larval morphology is given by using both traditional drawing techniques and macro multifocal image acquisition, combined with scanning electron microscopy (SEM) for analyzing the details. The diagnostic characters of these larvae are compared to those of the other known larvae of ozaenini.

Third instar larva morphology of Hydrotaea cyrtoneurina (ZETTERSTEDT, 1845) (Diptera: Muscidae) – a species of forensic interest

2013 ◽  
Vol 82 (4) ◽  
pp. 303-315 ◽  
Author(s):  
Andrzej Grzywacz
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Instar Larva ◽  
Palaearctic Species ◽  
Larval Morphology ◽  
The Third ◽  
Third Instar Larva ◽  
Scanning Electron ◽  
Third Instar Larvae

ABSTRACT The muscid Hydrotaea cyrtoneurina (ZETTERSTEDT, 1845) is a Palaearctic species whose adults are known for their forensic importance. The third instar larva morphology of H. cyrtoneurina, hitherto unknown, is described herein with the application of light and scanning electron microscopy. The characters of larval morphology enabling this species to be distinguished from other forensically important third instar larvae of Muscidae are discussed as well. Some details of the cephaloskeleton are discussed, and modifications in terminology are proposed based on the literature data and the results of this study.

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