scholarly journals Efficient and specific DNA oligonucleotide rRNA probe-based rRNA removal in Talaromyces marneffei

2022 ◽  
pp. 1-13
Author(s):  
Xueyan Hu ◽  
Yun Zhang ◽  
Minghao Du ◽  
Ence Yang
Keyword(s):  
Talaromyces Marneffei

scholarly journals The novel Dbl homology/BAR domain protein, MsgA, of Talaromyces marneffei regulates yeast morphogenesis during growth inside host cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Harshini Weerasinghe ◽  
Hayley E. Bugeja ◽  
Alex Andrianopoulos
Keyword(s):  
Pathogenic Fungi ◽  
Host Cells ◽  
Microbial Pathogens ◽  
Mammalian Host ◽  
Host Immune System ◽  
Nucleotide Exchange ◽  
Phagocytic Cells ◽  
Macrophage Infection ◽  
Bar Domain ◽  
Talaromyces Marneffei

AbstractMicrobial pathogens have evolved many strategies to evade recognition by the host immune system, including the use of phagocytic cells as a niche within which to proliferate. Dimorphic pathogenic fungi employ an induced morphogenetic transition, switching from multicellular hyphae to unicellular yeast that are more compatible with intracellular growth. A switch to mammalian host body temperature (37 °C) is a key trigger for the dimorphic switch. This study describes a novel gene, msgA, from the dimorphic fungal pathogen Talaromyces marneffei that controls cell morphology in response to host cues rather than temperature. The msgA gene is upregulated during murine macrophage infection, and deletion results in aberrant yeast morphology solely during growth inside macrophages. MsgA contains a Dbl homology domain, and a Bin, Amphiphysin, Rvs (BAR) domain instead of a Plekstrin homology domain typically associated with guanine nucleotide exchange factors (GEFs). The BAR domain is crucial in maintaining yeast morphology and cellular localisation during infection. The data suggests that MsgA does not act as a canonical GEF during macrophage infection and identifies a temperature independent pathway in T. marneffei that controls intracellular yeast morphogenesis.

A norbisabolane and an arabitol benzoate from Talaromyces marneffei, an endophytic fungus of Epilobium angustifolium

Fitoterapia ◽  
2021 ◽  
pp. 104948
Author(s):  
Zhong-Duo Yang ◽  
Xu-Dong Zhang ◽  
Xing Yang ◽  
Xiao-Jun Yao ◽  
Zong-Mei Shu
Keyword(s):  
Endophytic Fungus ◽  
Talaromyces Marneffei ◽  
Epilobium Angustifolium

scholarly journals HIV-negative case of Talaromyces marneffei pulmonary infection with a TSC2 mutation

2021 ◽  
Vol 49 (5) ◽  
pp. 030006052110167
Author(s):  
Qian Shen ◽  
Lingyan Sheng ◽  
Jianying Zhou
Keyword(s):  
Pulmonary Infection ◽  
Pathogenic Fungus ◽  
Critical Role ◽  
Lavage Fluid ◽  
Tsc2 Gene ◽  
Current Case ◽  
Negative Patient ◽  
Talaromyces Marneffei ◽  
Negative Case ◽  
Hiv Negative

Talaromyces marneffei is a rare dimorphic pathogenic fungus that can induce severe infections in human immunodeficiency virus (HIV)-infected patients. However, such infections have also been reported in non-HIV hosts. This current case report describes a very rare case of a T. marneffei pulmonary infection in an HIV-negative patient with a mutation in the tuberous sclerosis complex subunit 2 ( TSC2) gene. A 24-year-old male patient presented with cough and expectoration for 6 months. Computed tomography showed multiple ground-glass opacities and cystic and cavitated lesions in both lungs. Next generation sequencing (NGS) of the bronchoalveolar lavage fluid was performed to confirm T. marneffei pulmonary infection. The results were further verified using bronchoscopy specimen cultures. This was an HIV-negative patient without a travel history to endemic zones and his blood exon sequencing results showed a mutation in the TSC2 gene. To date, he has recovered well with voriconazole therapy. In summary, patients with TSC2 mutations that induce bronchopulmonary dysplasia may be potential hosts for T. marneffei. Early and timely diagnosis is important for improving prognosis. NGS plays a critical role in the diagnosis of T. marneffei pulmonary infection.

scholarly journals Talaromikosis Marneffei

2019 ◽  
Vol 12 (2) ◽  
pp. 72
Author(s):  
Sem S. Surja ◽  
Robiatul Adawiyah ◽  
Retno Wahyuningsih
Keyword(s):  
Endemic Area ◽  
Clinical Manifestations ◽  
Its Region ◽  
Clinical Suspicion ◽  
Cross Wall ◽  
Respiratory Disorder ◽  
Fungal Culture ◽  
Talaromyces Marneffei ◽  
Mycotic Disease ◽  
Histopathology Examination

Talaromycosis marneffei is a mycotic disease caused by Talaromyces marneffei which primarily infectimmunocompromised patient. This disease is endemic in Southeast Asia, and Indonesia consider as endemic area dueto a case of tourist who got the infection after visiting Indonesia. Diagnosis was made based on clinical suspicion withlaboratory confirmation. Clinical manifestations are not specific, such as fever, anemia, weight loss, lymphadenopathy,hepatomegaly, splenomegaly, respiratory disorder, and cutaneous manifestation. Histopathology and culture are themost common examination performed for diagnosis. In histopathology examination, fungi appear as fission arthroconidiacells which shape round to oval, with cross wall formation inside or outside macrophage and histiocyte. Thermaldimorphism characteristic can be observed in fungal culture. Fungi grows as mold at 25oC-30oC and as yeast at 37oC.Definitive identification of T. marneffei was done by molecular examination using primers derived from ITS region asprimary marker and beta tubulin region as secondary marker. Talaromyces marneffei is sapronosis, that transmitted viainhalation of conidia from environment.

scholarly journals Innate Immunity Acts as the Major Regulator in Talaromyces marneffei Coinfected AIDS Patients: Cytokine Profile Surveillance During Initial 6-Month Antifungal Therapy

2019 ◽  
Vol 6 (6) ◽  
Author(s):  
Rong-Jing Dong ◽  
Yun-Gui Zhang ◽  
Lei Zhu ◽  
Heng-Li Liu ◽  
Jun Liu ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Antifungal Therapy ◽  
Immune Cell ◽  
Host Immune System ◽  
Aids Patients ◽  
Fungus Infection ◽  
Talaromyces Marneffei ◽  
Tnf Α ◽  
Laboratory Examinations ◽  
Poor Outcomes

Abstract Background Talaromycosis caused by Talaromyces marneffei infection is a fatal systemic mycosis in immunosuppressed individuals, such as patients with AIDS. Cytokines and immunocytes play a central role against fungus infection. However, how the host immune system responds to infection and treatment has not been reported to date. Methods Forty-one Talaromyces marneffei coinfected AIDS patients were followed up, their immunocytes and cytokine profiles were obtained at different antifungal treatment stages, and data on clinical features and laboratory examinations were collected. Correlation analysis was used to identify factors associated with host immunity against Talaromyces marneffei infection in AIDS patients. Results Common diseases and conditions of these 41 patients were lymphadenopathy, hepatomegaly, and splenomegaly. CD4+ T cells were extremely low in all of them. Moreover, significant increases of proinflammatory cytokines (IL-12, IL-17A, TNF-α, IFN-γ, IL-18, and IL-1β), anti-inflammatory cytokines (IL-10), and chemokines (IP-10) were observed in talaromycosis before treatment (P < .05), comparing to both AIDS patients and healthy controls. The cytokines IL-6, IL-8, TNF-α, IL-18, IL-17A, IL-7, IP-10, and IL-1β reached peak levels 3 days after initial antifungal therapy, and then gradually decreased. The symptoms of the patients gradually decreased. Furthermore, patients who died showed the highest levels of IL-6, TNF-α, IL-8, IL-1β, and IP-10, which were 1.4- to 164-fold higher than in surviving patients. Conclusions Our findings indicate that innate immune-cell-derived cytokines are critical for host defense against AIDS-associated Talaromyces marneffei infection; furthermore, excessive inflammatory cytokines are associated with poor outcomes.

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