Comparative secretome analysis of Staphylococcus aureus strains with different within-herd intramammary infection prevalence

SummaryFifty-seven inoculations of a strain of Staphylococcus aureus were made into the outer 4 mm of the teat ducts of 8 cows. The inocula ranged from 10 to 600 colony-forming units (CFU) and were made with a stainless steel instrument designed to overcome the disadvantages of glass rods and cotton swabs hitherto used.A single inoculation resulted in either no colonization of the duct, in temporary colonization for up to 7 days, or in colonization followed by intramammary infection.The recovery of the organisms depended on the size of the inoculum. When 70–100 CFU were placed in the teat duct no organisms were recovered from 12 of 24 quarters after the 1st post-inoculation milking. When 500–600 CFU were used, organisms were recovered from all of 31 quarters for at least 3 milkings, and from 61% for 6 milkings or more, in spite of dipping the teats in a strong disinfectant twice daily.Intramammary infection developed in 1 of 12 quarters (8%) inoculated in the teat duct with about 600 CFU when the animals were milked twice daily, and in 5 of 19 (23%) quarters if the 1st post-inoculation milking was omitted.There was no evidence of sensitization resulting from previous infection.

Download Full-text

Experimental intramammary infection of ewes with Staphylococcus aureus subsp anaerobius

Research in Veterinary Science ◽

10.1016/0034-5288(93)90061-j ◽

1993 ◽

Vol 54 (2) ◽

pp. 221-226 ◽

Cited By ~ 5

Author(s):

R. De la Fuente ◽

J.A. Ruiz Santa Quiteria ◽

D. Cid ◽

M. Domingo ◽

G. Suarez

Keyword(s):

Staphylococcus Aureus ◽

Intramammary Infection

Download Full-text

The effect of intramuscular and intramammary vaccination of cows on antibody levels and resistance to intramammary infection by Staphylococcus aureus

Research in Veterinary Science ◽

10.1016/s0034-5288(18)33525-2 ◽

1975 ◽

Vol 19 (2) ◽

pp. 152-158 ◽

Cited By ~ 24

Author(s):

J.H. Brock ◽

E.D. Steel ◽

B. Reiter

Keyword(s):

Staphylococcus Aureus ◽

Intramammary Infection ◽

Antibody Levels

Download Full-text

Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows

Journal of Dairy Research ◽

10.1017/s0022029919001018 ◽

2020 ◽

Vol 87 (1) ◽

pp. 82-88

Author(s):

Cecilia M. Camussone ◽

Ana I. Molineri ◽

Marcelo L. Signorini ◽

Verónica E. Neder ◽

Carlos A. Vitulich ◽

...

Keyword(s):

Risk Factors ◽

Staphylococcus Aureus ◽

Management Practices ◽

Molecular Level ◽

Univariate Analysis ◽

Multivariable Analysis ◽

Cow Milk ◽

Intramammary Infection ◽

Dairy Heifers ◽

Lactating Cows

AbstractThe aims of the research reported here were to identify potential risk factors associated with the presence of Staphylococcus aureus intramammary infection (IMI) in pre partum dairy heifers on 17 dairy farms from three provinces of Argentina and to characterize, at molecular level, isolates from those heifers and lactating cows from two selected herds. A total of 1474 heifers and 4878 lactating cows were studied. The prevalence of Staphylococcus aureus IMI in the heifers, heifers at quarter level and lactating cow mammary quarters was 14.41, 4.82, and 14.65%, respectively. Univariate analysis showed the key variables associated with S. aureus IMI presence in the heifers were: S. aureus IMI prevalence in cows of the lactating herd, the time calves stayed with their dam after birth, the calf rearing system, the place of rearing (own farm or other dairy farm) and fly control on the farm. None of the variables included in the multivariable analysis was associated with the presence of S. aureus IMI in the pre partum heifers, probably due to low variability among management practices used by the farms for rearing the heifer calves. At the molecular level, S. aureus isolates were grouped into three main PFGE clusters and several genotypes within the clusters. Isolates from mammary secretion of pre partum heifers and milk of lactating cows comprised different PFGE clusters in both herds, although two exceptions occurred. The absence of gene fnbpB, which codifies for a virulence factor protein involved in cell invasion by S. aureus, was significantly more frequent in pre partum heifer secretion isolates than in isolates from lactating cow milk. These results suggest that, under these management conditions, isolates from mammary secretions of pre partum heifers do not originate from the milk of lactating cows, but rather other sources to which the heifer is exposed.

Download Full-text

Draft Genome Sequences of 27 Staphylococcus aureus Strains and 3 Staphylococcus Species Strains Isolated from Bovine Intramammary Infections

Microbiology Resource Announcements ◽

10.1128/mra.00300-20 ◽

2020 ◽

Vol 9 (19) ◽

Author(s):

Soyoun Park ◽

Dongyun Jung ◽

Simon Dufour ◽

Jennifer Ronholm

Keyword(s):

Staphylococcus Aureus ◽

Draft Genome ◽

Bovine Mastitis ◽

Holstein Cows ◽

Whole Genome ◽

Genome Sequences ◽

Content Type ◽

Intramammary Infections ◽

Intramammary Infection ◽

Etiological Agents

Staphylococcus aureus is one of the most common etiological agents responsible for contagious bovine mastitis. Here, we report the draft whole-genome sequences, with annotations, of 27 S. aureus strains and 3 Staphylococcus species strains that were isolated from Holstein cows with intramammary infection in Canada.

Download Full-text

Enzyme-Linked Immunosorbent Assay and Microbiologic Culture for Diagnosis of Staphylococcus aureus Intramammary Infection in Cows

Journal of Food Protection ◽

10.4315/0362-028x-59.1.6 ◽

1996 ◽

Vol 59 (1) ◽

pp. 6-10 ◽

Cited By ~ 3

Author(s):

PAUL C. BARTLETT ◽

RONALD J. ERSKINE ◽

PATRICK GASTON ◽

PHILIP M. SEARS ◽

HENDRICUS WILHELMUS HOUDIJK

Keyword(s):

Staphylococcus Aureus ◽

Sensitivity And Specificity ◽

Immunosorbent Assay ◽

Relative Sensitivity ◽

Elisa Test ◽

Enzyme Linked Immunosorbent Assay ◽

Infection Status ◽

Intramammary Infection ◽

Previous Infection ◽

Current Infection

Recent reports have indicated that the relative sensitivity and specificity of the ELISA test for detection of intramammary infection of cows with Staphylococcus aureus is not as high as originally reported. It has been suggested that antibodies measured by enzyme-linked immunosorbent assay (ELISA) more closely reflect previous infection status rather than current infection status, and that the delay in antibody formation following infection and the persistence of antibodies after elimination of infection may be responsible for some of the discrepancy observed between ELISA and bacterial culture results conducted on the same milk sample. This study (n = 209 cows) was undertaken to determine if an ELISA for S. aureus intramammary infection more closely reflects previous infection status than it does current infection status, and to ascertain whether correction of this time-delay factor substantially improves calculated values of ELISA relative sensitivity and specificity. Receiver-operator curves were constructed to compare different time-related definitions of microbiologic culture results used for comparison with ELISA results. A greater degree of curvature in receiver-operator curves indicated that ELISA results did more closely reflect culture results performed on milk samples taken 1 and 3 weeks previously. Insignificant improvement in sensitivity and specificity occurred when the database was limited to cows (n = 140) with milk production greater than 13.6 kg/day. However, values of sensitivity were all less than or equal to 90%, and values of specificity were all less than 54%.

Download Full-text