Cytotoxic Effects ofCombretum platypetalumWelw. ex M.A. Lawson subsp. oatesii (Rolfe) Exell (Combretaceae) Leaf Extracts on Jurkat T-cells and Reversal of Effects by Reduced Glutathione

2016 ◽  
Vol 6 (3) ◽  
pp. 250-265
Author(s):  
Orbert Chiramba ◽  
Stanley Mukanganyama
Keyword(s):  
T Cells ◽  
Reduced Glutathione ◽  
Leaf Extracts ◽  
Jurkat T Cells ◽  
Cytotoxic Effects

scholarly journals Antiproliferative Activity of the Isofuranonaphthoquinone Isolated fromBulbine frutescensagainst Jurkat T Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Penelope Tambama ◽  
Berhanu Abegaz ◽  
Stanley Mukanganyama
Keyword(s):  
T Cells ◽  
Anticancer Drug ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Synergistic Effects ◽  
Drug Efflux ◽  
Cellular Mechanisms ◽  
Jurkat T Cells ◽  
Public Health Burden ◽  
Drug Efflux Pumps

Cancer is a major public health burden in both developed and developing countries. The quinone moiety has been shown to possess antitumor activity and several cancer drugs in clinical use contain this entity. The effect of isofuranonaphthoquinone isolated fromBulbine frutescenson Jurkat T cells was determined. Cells were exposed to the isofuranonaphthoquinone (IFNQ) at different concentrations. Significant antiproliferative effects were observed which were comparable to that of the anticancer drug 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU). A combination of IFNQ with BCNU produced synergistic effects which were observed after 72 hrs. It was also observed that combining IFNQ with reduced glutathione abolished the anticancer activity of the compound. It is, therefore, proposed that the isofuranonaphthoquinone may exert part of its effect by producing reactive oxygen species resulting in death of the cells as the effects of this compound were antagonized by reduced glutathione. An investigation on the effects of isofuranonaphthoquinone on glutathione transferase (GST) activity and drug efflux pumps showed that this compound exhibited inhibitory effects on both the GST and the drug efflux pumping activities. Thus, the isofuranonaphthoquinone showed cytotoxicity, works through inhibition of some cellular mechanisms, and could present a potential source of lead compounds for anticancer drug development.

scholarly journals Anti-allergic and Cytotoxic Effects of Sesquiterpenoids and Phenylpropanoids Isolated from Magnolia biondii

2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Thi Tuyet Mai Nguyen ◽  
Thi Thu Nguyen ◽  
Hyun-Su Lee ◽  
Bomi Lee ◽  
Byung Sun Min ◽  
...  
Keyword(s):  
T Cells ◽  
Optical Rotation ◽  
Interleukin 2 ◽  
2D Nmr ◽  
Inhibitory Effect ◽  
2D Nmr Spectroscopy ◽  
Jurkat T Cells ◽  
Cytotoxic Effects ◽  
D 12 ◽  
Mcf 7

Ten sesquiterpenoids (1-10) and six phenylpropanoid derivatives (11-16) were isolated from the flower buds of Magnolia biondii Pamp. Their structures were determined by 1D- and 2D-NMR spectroscopy, mass spectrometry, and optical rotation. To evaluate their anti-allergic properties, the inhibitory effect of each isolate (1-16) on interleukin-2 (IL-2) gene expression was examined in Jurkat T cells. Among the isolated compounds, three sesquiterpenoids (2, 5, and 7) and two monoterpenoids (12 and 13) strongly inhibited IL-2 production in Jurkat T cells. Four compounds, (-)-parthenolide (2), eudesm-4(15)-eno-1β,6α-diol (5), biondinin D (12), and tiliroside (16), showed cytotoxicity against Jurkat T cells. In addition, (-)-parthenolide (2) exhibited cytotoxicity against the human cervical cancer HeLa cell line, the human breast cancer MCF-7, and human promyelocytic leukemia HL-60 cell lines.

scholarly journals Phytochemical Fingerprinting and Activity of Extracts from the Leaves of Dolichos kilimandscharicus (Fabaceae) on Jurkat-T Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Simbarashe Sithole ◽  
Paul Mushonga ◽  
Llewellyn N. R. Nhamo ◽  
Godloves Fru Chi ◽  
Stanley Mukanganyama
Keyword(s):  
T Cells ◽  
Ethyl Acetate ◽  
Prescription Drugs ◽  
Raw 264.7 Cells ◽  
Supporting Evidence ◽  
Leaf Extracts ◽  
Jurkat T Cells ◽  
Trypan Blue Exclusion ◽  
Antiproliferative Effects

Plants are a source of over a quarter of the prescription drugs currently in use worldwide. Zimbabwe has a rich plant biodiversity with only a limited number reported for the treatment of cancer. The leaf extracts of Dolichos kilimandscharicus were selected for the screening of their antiproliferative efficacy and cytotoxicity effects. This plant has increasingly been used by local folk as a treatment for cancer or cancer-related symptoms though its bioactivity has not been scientifically determined. This investigation also sought to identify constituent compounds in the crude extract preparations responsible for their antiproliferative efficacy. The antiproliferative effects of six-leaf extracts on Jurkat-T in vitro were investigated using the Trypan blue exclusion assay. The extracts were tested with increasing concentration, using chlorambucil as a standard anticancer drug. Cytotoxicity of extracts was determined against RAW 264.7 cells using a colorimetric tetrazolium-based assay. In additionthe ability of the extracts to induce apoptosis was determined for the most potent leaf extracts. The order of potency of the leaf extracts of D. kilimandscharicus against Jurkat-T cell line was found to be MeOH < Ethyl Acetate < DCM: MeOH < EtOH with IC50s of 33.56, 30.44, 22.93, and 21.59 μg/mL, respectively. Furthermore, the most potent extracts exhibited very low cytotoxicity against all the tested cells. D. kilimandscharicus leaf extracts induced apoptosis in the Jurkat-T cells as was shown by DNA fragmentation. UPLC-MS analysis of crude extracts led to the identification of 23 compounds from the ethanol extract and these may be responsible for the observed antiproliferative effects. Rutin, quercetin, luteolin, apigenin, hispidulin, kaempferol derivatives, as well as caffeoylquinic acid are some of the compounds identified in the extracts. The results of this study showed that the ethanol and ethyl acetate leaf extracts of D. kilimandscharicus have antiproliferative activity against Jurkat-T cells and may act by inducing apoptosis.. The current findings offer supporting evidence for the use of these plant species in the treatment of cancer in ethnomedicinal practices.

scholarly journals Antiproliferative Effect of Green Papaya on Lymphocytic Leukemic Cells

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Zainab Allqmani ◽  
Alexus Greene ◽  
Haiwen Li ◽  
Rafie Reza ◽  
Andrea R Beyer ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cells ◽  
Lymphocytic Leukemia ◽  
New Drugs ◽  
Gastric Ulcers ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Jurkat T Cells ◽  
Oxidation Activity ◽  
Water Extracts

Numerous edible plants have been reported to interfere with the carcinogenic process, and therefore, the regular consumption of these plant products may reduce the risk of developing cancer. We investigated the effect of papaya fruit and leaves on the cell proliferation of Jurkat T-lymphocytic and Daudi B-lymphocytic leukemia cells. Cells were treated with aqueous or methanolic extracts from leaves, skin, pulp, and seeds from green papaya. The papaya fractions were tested for total phenolic content, total flavonoids content, and anti-oxidation activity using chemical assays. Cell proliferation was measured using a WST-1 assay. Our data indicate that methanol and water extracts of seeds and leaves contained higher concentrations of total phenolic compounds and higher anti-oxidation activity than that of extracts from skin and pulp. Both methanol and water extracts from leaves and skin potently inhibited the proliferation of leukemic Jurkat T-cells and Daudi B-cells. However, the effect was more potent on Jurkat T-cells, and the leaf extracts were more effective than that of skin extracts. None of the pulp or seed extracts showed inhibitory activity on leukemic cell proliferation. Although papaya leaves are not consumed as a food, leaf extracts have been used for the treatment of various conditions, including dengue and malaria fevers, gastric ulcers, low platelet counts, and cancers of the breast, lung, and cervix. Our data suggest that the consumption of papaya leaf extracts may also be beneficial in preventing and/or treating lymphocytic cancer. Isolation of active compounds from papaya leaves will also help in developing new drugs for cancer treatment.

scholarly journals The Effects of Combining Cancer Drugs with Compounds Isolated from Combretum zeyheri Sond. and Combretum platypetalum Welw. ex M.A. Lawson (Combretaceae) on the Viability of Jurkat T Cells and HL-60 Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Morris Wende ◽  
Simbarashe Sithole ◽  
Godloves Fru Chi ◽  
Marc Y. Stevens ◽  
Stanley Mukanganyama
Keyword(s):  
T Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mammalian Cells ◽  
Cancer Cell Lines ◽  
Jurkat T Cells ◽  
Cytotoxic Effects ◽  
Peritoneal Cells

Combretum zeyheri and Combretum platypetalum have been shown to have anticancer, antibacterial, antituberculosis, and antifungal effects in both in vivo and in vitro studies. This study sought to evaluate the antiproliferative effects of compounds isolated from C. zeyheri and C. platypetalum on Jurkat T and HL-60 cancer cell lines in combination with doxorubicin and/or chlorambucil. At their GI50 concentrations, the isolated compounds were combined with the corresponding GI50 of chlorambucil and doxorubicin. The cytotoxic effects of the combined compounds were determined on BALB/c mouse peritoneal cells. All the 4 isolated compounds had significant cytotoxic effects on Jurkat T cells. Compounds CP 404 (1), CP 409 (2), CZ 453 (3), and CZ 455 (4) had GI50s on Jurkat T cells of 3.98, 19.33, 6.82, and 20.28 μg/ml, respectively. CP 404 (1), CP 409 (2), CZ 453 (3), and CZ 455 (4) showed GI50s of 14.18, 28.69, 29.87, and 16.46 μg/ml on HL-60 cancer cell lines, respectively. The most potent combination against Jurkat T cells was found to be CP 404 (1) and chlorambucil. This combination showed no cytotoxic effects when tested on BALB/c mouse peritoneal cells. It was concluded that the compounds extracted from C. zeyheri and C. platypetalum inhibit the growth of Jurkat T cells in vitro. The combination of the compounds with anticancer drugs enhanced their anticancer effects. The combination of CP 404 (1) and chlorambucil was found not to be toxic to normal mammalian cells. Therefore, CP 404 (1), 3-O-β-L-rrhamnopyranosyl-5,7,3 ′ 4 ′ ,5 ′ -pentahydroxyflavone, has the potential to be a source of lead compounds that can be developed for anticancer therapy. Further structure-activity relationship studies on this compound are warranted.

The role of cbl family of ubiquitin ligases in gastric cancer exosome-induced apoptosis of Jurkat T cells

2009 ◽  
pp. 1-8
Author(s):  
Jing-Lei Qu ◽  
Xiu-Juan Qu ◽  
Ming-Fang Zhao ◽  
Yue-E Teng ◽  
Ye Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
T Cells ◽  
Ubiquitin Ligases ◽  
Jurkat T Cells ◽  
Induced Apoptosis

Indicators of the antioxidant system in the long-term period after acute mercury nitrate poisoning in the experiment

2020 ◽  
pp. 36-41
Author(s):  
E. G. Batotsyrenova ◽  
O. A. Vakunenkova ◽  
E. A. Zolotoverkhaya ◽  
V. A. Kashuro ◽  
T. A. Kostrova ◽  
...  
Keyword(s):  
Antioxidant System ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Antioxidant Defense System ◽  
Mercury Content ◽  
The State ◽  
Cytotoxic Effects ◽  
Entire Study ◽  
Mercury Nitrate

The article presents experimental data on the state of the antioxidant system in red blood cells of white outbred rats 1 and 3 months after acute mercury nitrate poisoning with a semilethal dose. It has been established that this form of intoxication is accompanied by pronounced changes in the state of the antioxidant defense system in erythrocytes of poisoned animals (a decrease in the concentration of reduced glutathione, a decrease in the activity of superoxide dismutase and glutathione peroxidase, and an increase in the concentration of lipid peroxidation products).It has been shown that the mercury content in the blood of experimental animals remains elevated during the entire study period.The results obtained indicate the importance of impaired functioning of the antioxidant system in the implementation of long-term consequences of acute mercury poisoning. The reasons for the occurrence of these biochemical shifts and their role in the development of the long-term cytotoxic effects of mercury nitrate are discussed.

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